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Biological warfare, also known as germ warfare, is the use of biological toxins or infectious agents such as
bacteria Bacteria (; : bacterium) are ubiquitous, mostly free-living organisms often consisting of one Cell (biology), biological cell. They constitute a large domain (biology), domain of Prokaryote, prokaryotic microorganisms. Typically a few micr ...
,
virus A virus is a submicroscopic infectious agent that replicates only inside the living Cell (biology), cells of an organism. Viruses infect all life forms, from animals and plants to microorganisms, including bacteria and archaea. Viruses are ...
es,
insects Insects (from Latin ') are hexapod invertebrates of the class Insecta. They are the largest group within the arthropod phylum. Insects have a chitinous exoskeleton, a three-part body (head, thorax and abdomen), three pairs of jointed ...
, and
fungi A fungus (: fungi , , , or ; or funguses) is any member of the group of eukaryotic organisms that includes microorganisms such as yeasts and mold (fungus), molds, as well as the more familiar mushrooms. These organisms are classified as one ...
with the intent to kill, harm or incapacitate humans, animals or plants as an act of war. Biological weapons (often termed "bio-weapons", "biological threat agents", or "bio-agents") are living
organism An organism is any life, living thing that functions as an individual. Such a definition raises more problems than it solves, not least because the concept of an individual is also difficult. Many criteria, few of them widely accepted, have be ...
s or replicating entities (i.e. 
virus A virus is a submicroscopic infectious agent that replicates only inside the living Cell (biology), cells of an organism. Viruses infect all life forms, from animals and plants to microorganisms, including bacteria and archaea. Viruses are ...
es, which are not universally considered "alive"). Entomological (insect) warfare is a subtype of biological warfare. Biological warfare is subject to a forceful normative prohibition. Offensive biological warfare in international armed conflicts is a
war crime A war crime is a violation of the laws of war that gives rise to individual criminal responsibility for actions by combatants in action, such as intentionally killing civilians or intentionally killing prisoners of war, torture, taking hostage ...
under the 1925 Geneva Protocol and several
international humanitarian law International humanitarian law (IHL), also referred to as the laws of armed conflict or the laws of war, is the law that regulates the conduct of war (''wikt:jus in bello, jus in bello''). It is a branch of international law that seeks to limit ...
treaties A treaty is a formal, legally binding written agreement between sovereign states and/or international organizations that is governed by international law. A treaty may also be known as an international agreement, protocol, covenant, convention ...
. In particular, the 1972
Biological Weapons Convention The Biological Weapons Convention (BWC), or Biological and Toxin Weapons Convention (BTWC), is a disarmament treaty that effectively bans Biological weapons, biological and toxin weapons by prohibiting their development, production, acquisition, ...
(BWC) bans the development, production, acquisition, transfer, stockpiling and use of biological weapons. In contrast, defensive biological research for prophylactic, protective or other peaceful purposes is not prohibited by the BWC. Biological warfare is distinct from warfare involving other types of
weapons of mass destruction A weapon of mass destruction (WMD) is a Biological agent, biological, chemical weapon, chemical, Radiological weapon, radiological, nuclear weapon, nuclear, or any other weapon that can kill or significantly harm many people or cause great dam ...
(WMD), including
nuclear warfare Nuclear warfare, also known as atomic warfare, is a War, military conflict or prepared Policy, political strategy that deploys nuclear weaponry. Nuclear weapons are Weapon of mass destruction, weapons of mass destruction; in contrast to conven ...
, chemical warfare, and radiological warfare. None of these are considered conventional weapons, which are deployed primarily for their
explosive An explosive (or explosive material) is a reactive substance that contains a great amount of potential energy that can produce an explosion if released suddenly, usually accompanied by the production of light, heat, sound, and pressure. An ex ...
, kinetic, or incendiary potential. Biological weapons may be employed in various ways to gain a strategic or tactical advantage over the enemy, either by threats or by actual deployments. Like some chemical weapons, biological weapons may also be useful as area denial weapons. These agents may be lethal or non-lethal, and may be targeted against a single individual, a group of people, or even an entire population. They may be developed, acquired, stockpiled or deployed by
nation state A nation state, or nation-state, is a political entity in which the State (polity), state (a centralized political organization ruling over a population within a territory) and the nation (a community based on a common identity) are (broadly ...
s or by non-national groups. In the latter case, or if a nation-state uses it clandestinely, it may also be considered bioterrorism. Biological warfare and chemical warfare overlap to an extent, as the use of
toxin A toxin is a naturally occurring poison produced by metabolic activities of living cells or organisms. They occur especially as proteins, often conjugated. The term was first used by organic chemist Ludwig Brieger (1849–1919), derived ...
s produced by some living organisms is considered under the provisions of both the BWC and the Chemical Weapons Convention. Toxins and psychochemical weapons are often referred to as ''midspectrum agents''. Unlike bioweapons, these midspectrum agents do not reproduce in their host and are typically characterized by shorter incubation periods.


Overview

A biological attack could conceivably result in large numbers of civilian casualties and cause severe disruption to
economic An economy is an area of the Production (economics), production, Distribution (economics), distribution and trade, as well as Consumption (economics), consumption of Goods (economics), goods and Service (economics), services. In general, it is ...
and societal infrastructure. A nation or group that can pose a credible threat of mass casualty has the ability to alter the terms under which other nations or groups interact with it. When indexed to weapon mass and cost of development and storage, biological weapons possess destructive potential and loss of life far in excess of nuclear, chemical or conventional weapons. Accordingly, biological agents are potentially useful as strategic deterrents, in addition to their utility as offensive weapons on the battlefield. As a tactical weapon for military use, a significant problem with biological warfare is that it would take days to be effective, and therefore might not immediately stop an opposing force. Some biological agents (
smallpox Smallpox was an infectious disease caused by Variola virus (often called Smallpox virus), which belongs to the genus '' Orthopoxvirus''. The last naturally occurring case was diagnosed in October 1977, and the World Health Organization (W ...
, pneumonic plague) have the capability of person-to-person transmission via aerosolized respiratory droplets. This feature can be undesirable, as the agent(s) may be transmitted by this mechanism to unintended populations, including neutral or even friendly forces. Worse still, such a weapon could "escape" the laboratory where it was developed, even if there was no intent to use it – for example by infecting a researcher who then transmits it to the outside world before realizing that they were infected. Several cases are known of researchers becoming infected and dying of
Ebola Ebola, also known as Ebola virus disease (EVD) and Ebola hemorrhagic fever (EHF), is a viral hemorrhagic fever in humans and other primates, caused by ebolaviruses. Symptoms typically start anywhere between two days and three weeks after in ...
, which they had been working with in the lab (though nobody else was infected in those cases) – while there is no evidence that their work was directed towards biological warfare, it demonstrates the potential for accidental infection even of careful researchers fully aware of the dangers. While containment of biological warfare is less of a concern for certain criminal or terrorist organizations, it remains a significant concern for the military and civilian populations of virtually all nations.


History


Antiquity and Middle Ages

Rudimentary forms of biological warfare have been practiced since antiquity. The earliest documented incident of the intention to use biological weapons is recorded in Hittite texts of 1500–1200 BC, in which victims of an unknown plague (possibly tularemia) were driven into enemy lands, causing an epidemic. The Assyrians poisoned enemy wells with the fungus ergot, though with unknown results. Scythian archers dipped their arrows and Roman soldiers their swords into excrements and cadavers – victims were commonly infected by tetanus as result. In 1346, the bodies of Mongol warriors of the Golden Horde who had died of plague were thrown over the walls of the besieged Crimean city of Kaffa. Specialists disagree about whether this operation was responsible for the spread of the
Black Death The Black Death was a bubonic plague pandemic that occurred in Europe from 1346 to 1353. It was one of the list of epidemics, most fatal pandemics in human history; as many as people perished, perhaps 50% of Europe's 14th century population. ...
into Europe, Near East and North Africa, resulting in the deaths of approximately 25 million Europeans. Biological agents were extensively used in many parts of Africa from the sixteenth century AD, most of the time in the form of poisoned arrows, or powder spread on the war front as well as poisoning of horses and water supply of the enemy forces. In Borgu, there were specific mixtures to kill, hypnotize, make the enemy bold, and to act as an antidote against the poison of the enemy as well. The creation of biologicals was reserved for a specific and professional class of medicine-men.


18th to 19th century

During the
French and Indian War The French and Indian War, 1754 to 1763, was a colonial conflict in North America between Kingdom of Great Britain, Great Britain and Kingdom of France, France, along with their respective Native Americans in the United States, Native American ...
, in June 1763 a group of Native Americans laid
siege A siege () . is a military blockade of a city, or fortress, with the intent of conquering by attrition, or by well-prepared assault. Siege warfare (also called siegecrafts or poliorcetics) is a form of constant, low-intensity conflict charact ...
to British-held Fort Pitt. Following instructions of his superior, Colonel Henry Bouquet, the commander of Fort Pitt, Swiss-born Captain Simeon Ecuyer, ordered his men to take smallpox-infested blankets from the infirmary and give it to a Lenape delegation during the siege. A reported outbreak that began the spring before left as many as one hundred Native Americans dead in Ohio Country from 1763 to 1764. It is not clear whether the smallpox was a result of the Fort Pitt incident or the virus was already present among the Delaware people as outbreaks happened on their own every dozen or so years and the delegates were met again later and seemingly had not contracted smallpox. During the
American Revolutionary War The American Revolutionary War (April 19, 1775 – September 3, 1783), also known as the Revolutionary War or American War of Independence, was the armed conflict that comprised the final eight years of the broader American Revolution, in which Am ...
,
Continental Army The Continental Army was the army of the United Colonies representing the Thirteen Colonies and later the United States during the American Revolutionary War. It was formed on June 14, 1775, by a resolution passed by the Second Continental Co ...
officer
George Washington George Washington (, 1799) was a Founding Fathers of the United States, Founding Father and the first president of the United States, serving from 1789 to 1797. As commander of the Continental Army, Washington led Patriot (American Revoluti ...
mentioned to the Continental Congress that he had heard a rumor from a sailor that his opponent during the Siege of Boston, General William Howe, had deliberately sent civilians out of the city in the hopes of spreading the ongoing smallpox epidemic to American lines; Washington, remaining unconvinced, wrote that he "could hardly give credit to" the claim. Washington had already inoculated his soldiers, diminishing the effect of the epidemic. Some historians have claimed that a detachment of the Corps of Royal Marines stationed in
New South Wales New South Wales (commonly abbreviated as NSW) is a States and territories of Australia, state on the Eastern states of Australia, east coast of :Australia. It borders Queensland to the north, Victoria (state), Victoria to the south, and South ...
, Australia, deliberately used
smallpox Smallpox was an infectious disease caused by Variola virus (often called Smallpox virus), which belongs to the genus '' Orthopoxvirus''. The last naturally occurring case was diagnosed in October 1977, and the World Health Organization (W ...
there in 1789. Dr Seth Carus states: "Ultimately, we have a strong circumstantial case supporting the theory that someone deliberately introduced smallpox in the Aboriginal population."


World War I

By 1900 the
germ theory The germ theory of disease is the currently accepted scientific theory for many diseases. It states that microorganisms known as pathogens or "germs" can cause disease. These small organisms, which are too small to be seen without magnification, ...
and advances in
bacteriology Bacteriology is the branch and specialty of biology that studies the Morphology (biology), morphology, ecology, genetics and biochemistry of bacteria as well as many other aspects related to them. This subdivision of microbiology involves the iden ...
brought a new level of sophistication to the techniques for possible use of bio-agents in war. Biological sabotage in the form of
anthrax Anthrax is an infection caused by the bacterium '' Bacillus anthracis'' or ''Bacillus cereus'' biovar ''anthracis''. Infection typically occurs by contact with the skin, inhalation, or intestinal absorption. Symptom onset occurs between one ...
and
glanders Glanders is a contagious, zoonotic infectious disease caused by the bacterium '' Burkholderia mallei'', which primarily occurs in horses, mules, and donkeys, but can also be contracted by dogs and cats, pigs, goats, and humans. The term ''glan ...
was undertaken on behalf of the Imperial German government during
World War I World War I or the First World War (28 July 1914 – 11 November 1918), also known as the Great War, was a World war, global conflict between two coalitions: the Allies of World War I, Allies (or Entente) and the Central Powers. Fighting to ...
(1914–1918), with indifferent results. The Geneva Protocol of 1925 prohibited the first use of chemical and biological weapons against enemy nationals in international armed conflicts.


World War II

With the onset of
World War II World War II or the Second World War (1 September 1939 – 2 September 1945) was a World war, global conflict between two coalitions: the Allies of World War II, Allies and the Axis powers. World War II by country, Nearly all of the wo ...
, the Ministry of Supply in the
United Kingdom The United Kingdom of Great Britain and Northern Ireland, commonly known as the United Kingdom (UK) or Britain, is a country in Northwestern Europe, off the coast of European mainland, the continental mainland. It comprises England, Scotlan ...
established a biological warfare program at Porton Down, headed by the microbiologist Paul Fildes. The research was championed by
Winston Churchill Sir Winston Leonard Spencer Churchill (30 November 1874 – 24 January 1965) was a British statesman, military officer, and writer who was Prime Minister of the United Kingdom from 1940 to 1945 (Winston Churchill in the Second World War, ...
and soon tularemia,
anthrax Anthrax is an infection caused by the bacterium '' Bacillus anthracis'' or ''Bacillus cereus'' biovar ''anthracis''. Infection typically occurs by contact with the skin, inhalation, or intestinal absorption. Symptom onset occurs between one ...
,
brucellosis Brucellosis is a zoonosis spread primarily via ingestion of raw milk, unpasteurized milk from infected animals. It is also known as undulant fever, Malta fever, and Mediterranean fever. The bacteria causing this disease, ''Brucella'', are small ...
, and
botulism Botulism is a rare and potentially fatal illness caused by botulinum toxin, which is produced by the bacterium ''Clostridium botulinum''. The disease begins with weakness, blurred vision, Fatigue (medical), feeling tired, and trouble speaking. ...
toxins had been effectively weaponized. In particular, Gruinard Island in Scotland, was contaminated with anthrax during a series of extensive tests for the next 56 years. Although the UK never offensively used the biological weapons it developed, its program was the first to successfully weaponize a variety of deadly pathogens and bring them into industrial production. Other nations, notably France and Japan, had begun their own biological weapons programs. When the United States entered the war, Allied resources were pooled at the request of the British. The US then established a large research program and industrial complex at Fort Detrick, Maryland, in 1942 under the direction of George W. Merck. The biological and chemical weapons developed during that period were tested at the Dugway Proving Grounds in
Utah Utah is a landlocked state in the Mountain states, Mountain West subregion of the Western United States. It is one of the Four Corners states, sharing a border with Arizona, Colorado, and New Mexico. It also borders Wyoming to the northea ...
. Soon there were facilities for the mass production of anthrax spores,
brucellosis Brucellosis is a zoonosis spread primarily via ingestion of raw milk, unpasteurized milk from infected animals. It is also known as undulant fever, Malta fever, and Mediterranean fever. The bacteria causing this disease, ''Brucella'', are small ...
, and
botulism Botulism is a rare and potentially fatal illness caused by botulinum toxin, which is produced by the bacterium ''Clostridium botulinum''. The disease begins with weakness, blurred vision, Fatigue (medical), feeling tired, and trouble speaking. ...
toxins, although the war was over before these weapons could be of much operational use. The most notorious program of the period was run by the secret
Imperial Japanese Army The Imperial Japanese Army (IJA; , ''Dai-Nippon Teikoku Rikugun'', "Army of the Greater Japanese Empire") was the principal ground force of the Empire of Japan from 1871 to 1945. It played a central role in Japan’s rapid modernization during th ...
Unit 731 , short for Manchu Detachment 731 and also known as the Kamo Detachment and the Ishii Unit, was a covert biological and chemical warfare research and development unit of the Imperial Japanese Army that engaged in lethal human experimentat ...
during the
war War is an armed conflict between the armed forces of states, or between governmental forces and armed groups that are organized under a certain command structure and have the capacity to sustain military operations, or between such organi ...
, based at Pingfan in
Manchuria Manchuria is a historical region in northeast Asia encompassing the entirety of present-day northeast China and parts of the modern-day Russian Far East south of the Uda (Khabarovsk Krai), Uda River and the Tukuringra-Dzhagdy Ranges. The exact ...
and commanded by Lieutenant General
Shirō Ishii Surgeon General was a Japanese microbiologist and Military medicine, army medical officer, who served as the director of Unit 731, a biological warfare unit of the Imperial Japanese Army. Ishii led the development and application of biological ...
. This biological warfare research unit conducted often fatal human experiments on prisoners, and produced biological weapons for combat use. Although the Japanese effort lacked the technological sophistication of the American or British programs, it far outstripped them in its widespread application and indiscriminate brutality. Biological weapons were used against Chinese soldiers and civilians in several military campaigns. In 1940, the Japanese Army Air Force bombed
Ningbo Ningbo is a sub-provincial city in northeastern Zhejiang province, People's Republic of China. It comprises six urban districts, two satellite county-level cities, and two rural counties, including several islands in Hangzhou Bay and the Eas ...
with ceramic bombs full of fleas carrying the bubonic plague. Many of these operations were ineffective due to inefficient delivery systems, although up to 200,000 people may have died. During the Zhejiang-Jiangxi Campaign in 1942, around 1,700 Japanese troops died out of a total 10,000 Japanese soldiers who fell ill with disease when their own biological weapons attack rebounded on their own forces. During the final months of World War II, Japan planned to use plague as a biological weapon against US civilians in
San Diego San Diego ( , ) is a city on the Pacific coast of Southern California, adjacent to the Mexico–United States border. With a population of over 1.4 million, it is the List of United States cities by population, eighth-most populous city in t ...
,
California California () is a U.S. state, state in the Western United States that lies on the West Coast of the United States, Pacific Coast. It borders Oregon to the north, Nevada and Arizona to the east, and shares Mexico–United States border, an ...
, during Operation Cherry Blossoms at Night. The plan was set to launch on 22 September 1945, but it was not executed because of Japan's surrender on 15 August 1945.


1948 Arab–Israeli War

According to historians
Benny Morris Benny Morris (; born 8 December 1948) is an Israeli historian. He was a professor of history in the Middle East Studies department of Ben-Gurion University of the Negev in the city of Beersheba, Israel. Morris was initially associated with the ...
and Benjamin Kedar, Israel conducted a biological warfare operation codenamed Operation Cast Thy Bread during the
1948 Arab–Israeli War The 1948 Arab–Israeli War, also known as the First Arab–Israeli War, followed the 1947–1948 civil war in Mandatory Palestine, civil war in Mandatory Palestine as the second and final stage of the 1948 Palestine war. The civil war becam ...
. The Haganah initially used typhoid bacteria to contaminate water wells in newly cleared Arab villages to prevent the population including militiamen from returning. Later, the biological warfare campaign expanded to include Jewish settlements that were in imminent danger of being captured by Arab troops and inhabited Arab towns not slated for capture. There was also plans to expand the biological warfare campaign into other Arab states including Egypt, Lebanon and Syria, but they were not carried out. Some British soldiers were also poisoned: causing the event to gain international attention.


Cold War

In Britain, the 1950s saw the weaponization of plague,
brucellosis Brucellosis is a zoonosis spread primarily via ingestion of raw milk, unpasteurized milk from infected animals. It is also known as undulant fever, Malta fever, and Mediterranean fever. The bacteria causing this disease, ''Brucella'', are small ...
, tularemia and later equine encephalomyelitis and
vaccinia The vaccinia virus (VACV or VV) is a large, complex, enveloped virus belonging to the poxvirus family. It has a linear, double-stranded DNA genome approximately 190 kbp in length, which encodes approximately 250 genes. The dimensions of the ...
viruses, but the programme was unilaterally cancelled in 1956. The United States Army Biological Warfare Laboratories weaponized
anthrax Anthrax is an infection caused by the bacterium '' Bacillus anthracis'' or ''Bacillus cereus'' biovar ''anthracis''. Infection typically occurs by contact with the skin, inhalation, or intestinal absorption. Symptom onset occurs between one ...
, tularemia,
brucellosis Brucellosis is a zoonosis spread primarily via ingestion of raw milk, unpasteurized milk from infected animals. It is also known as undulant fever, Malta fever, and Mediterranean fever. The bacteria causing this disease, ''Brucella'', are small ...
, Q-fever and others. In 1969, US President
Richard Nixon Richard Milhous Nixon (January 9, 1913April 22, 1994) was the 37th president of the United States, serving from 1969 until Resignation of Richard Nixon, his resignation in 1974. A member of the Republican Party (United States), Republican ...
decided to unilaterally terminate the offensive biological weapons program of the US, allowing only scientific research for defensive measures. This decision increased the momentum of the negotiations for a ban on biological warfare, which took place from 1969 to 1972 in the United Nation's Conference of the Committee on Disarmament in Geneva. These negotiations resulted in the
Biological Weapons Convention The Biological Weapons Convention (BWC), or Biological and Toxin Weapons Convention (BTWC), is a disarmament treaty that effectively bans Biological weapons, biological and toxin weapons by prohibiting their development, production, acquisition, ...
, which was opened for signature on 10 April 1972 and entered into force on 26 March 1975 after its ratification by 22 states. Despite being a party and depositary to the BWC, the
Soviet Union The Union of Soviet Socialist Republics. (USSR), commonly known as the Soviet Union, was a List of former transcontinental countries#Since 1700, transcontinental country that spanned much of Eurasia from 1922 until Dissolution of the Soviet ...
continued and expanded its massive offensive biological weapons program, under the leadership of the allegedly civilian institution Biopreparat. The Soviet Union attracted international suspicion after the 1979 Sverdlovsk anthrax leak killed approximately 65 to 100 people.


International law

International restrictions on biological warfare began with the 1925 Geneva Protocol, which prohibits the use but not the possession or development of biological and chemical weapons in international armed conflicts. Upon ratification of the Geneva Protocol, several countries made reservations regarding its applicability and use in retaliation. Due to these reservations, it was in practice a " no-first-use" agreement only. The 1972
Biological Weapons Convention The Biological Weapons Convention (BWC), or Biological and Toxin Weapons Convention (BTWC), is a disarmament treaty that effectively bans Biological weapons, biological and toxin weapons by prohibiting their development, production, acquisition, ...
(BWC) supplements the Geneva Protocol by prohibiting the development, production, acquisition, transfer, stockpiling and use of biological weapons. Having entered into force on 26 March 1975, the BWC was the first multilateral disarmament treaty to ban the production of an entire category of weapons of mass destruction. As of March 2021, 183 states have become party to the treaty. The BWC is considered to have established a strong global norm against biological weapons, which is reflected in the treaty's preamble, stating that the use of biological weapons would be "repugnant to the conscience of mankind". The BWC's effectiveness has been limited due to insufficient institutional support and the absence of any formal verification regime to monitor compliance. In 1985, the Australia Group was established, a multilateral export control regime of 43 countries aiming to prevent the proliferation of chemical and biological weapons. In 2004, the
United Nations Security Council The United Nations Security Council (UNSC) is one of the six principal organs of the United Nations (UN) and is charged with ensuring international peace and security, recommending the admission of new UN members to the General Assembly, an ...
passed Resolution 1540, which obligates all UN Member States to develop and enforce appropriate legal and regulatory measures against the proliferation of
chemical A chemical substance is a unique form of matter with constant chemical composition and characteristic properties. Chemical substances may take the form of a single element or chemical compounds. If two or more chemical substances can be combin ...
, biological,
radiological In physics, radiation is the emission or transmission of energy in the form of waves or particles through space or a material medium. This includes: * ''electromagnetic radiation'' consisting of photons, such as radio waves, microwaves, infr ...
, and
nuclear weapon A nuclear weapon is an explosive device that derives its destructive force from nuclear reactions, either fission (fission or atomic bomb) or a combination of fission and fusion reactions (thermonuclear weapon), producing a nuclear exp ...
s and their means of delivery, in particular, to prevent the spread of weapons of mass destruction to
non-state actor A non-state actor (NSA) is an individual or organization that has significant political influence but is not allied to any particular country or state. The interests, structure, and influence of NSAs vary widely. For example, among NSAs are non-pr ...
s.


Bioterrorism

Biological weapons are difficult to detect, economical and easy to use, making them appealing to terrorists. The cost of a biological weapon is estimated to be about 0.05 percent the cost of a conventional weapon in order to produce similar numbers of mass casualties per kilometer square. Moreover, their production is very easy as common technology can be used to produce biological warfare agents, like that used in production of vaccines, foods, spray devices, beverages and antibiotics. A major factor in biological warfare that attracts terrorists is that they can easily escape before the government agencies or secret agencies have even started their investigation. This is because the potential organism has an incubation period of 3 to 7 days, after which the results begin to appear, thereby giving terrorists a lead. A technique called Clustered, Regularly Interspaced, Short Palindromic Repeat ( CRISPR-Cas9) is now so cheap and widely available that scientists fear that amateurs will start experimenting with them. In this technique, a DNA sequence is cut off and replaced with a new sequence, e.g. one that codes for a particular protein, with the intent of modifying an organism's traits. Concerns have emerged regarding do-it-yourself biology research organizations due to their associated risk that a rogue amateur DIY researcher could attempt to develop dangerous bioweapons using genome editing technology. In 2002, when CNN went through Al-Qaeda's (AQ's) experiments with crude poisons, they found out that AQ had begun planning ricin and cyanide attacks with the help of a loose association of terrorist cells. The associates had infiltrated many countries like Turkey, Italy, Spain, France and others. In 2015, to combat the threat of bioterrorism, a National Blueprint for Biodefense was issued by the Blue-Ribbon Study Panel on Biodefense. Also, 233 potential exposures of select biological agents outside of the primary barriers of the biocontainment in the US were described by the annual report of the Federal Select Agent Program. Though a verification system can reduce bioterrorism, an employee, or a lone terrorist having adequate knowledge of a bio-technology company's facilities, can cause potential danger by using, without proper oversight and supervision, that company's resources. Moreover, it has been found that about 95% of accidents that have occurred due to low security have been done by employees or those who had a security clearance.


Entomology

Entomological warfare (EW) is a type of biological warfare that uses insects to attack the enemy. The concept has existed for centuries and research and development have continued into the modern era. EW has been used in battle by Japan and several other nations have developed and been accused of using an entomological warfare program. EW may employ insects in a direct attack or as vectors to deliver a
biological agent Biological agents, also known as biological weapons or bioweapons, are pathogens used as weapons. In addition to these living or replicating pathogens, toxins and Toxin#Biotoxins, biotoxins are also included among the bio-agents. More than 1,2 ...
, such as plague. Essentially, EW exists in three varieties. One type of EW involves infecting insects with a
pathogen In biology, a pathogen (, "suffering", "passion" and , "producer of"), in the oldest and broadest sense, is any organism or agent that can produce disease. A pathogen may also be referred to as an infectious agent, or simply a Germ theory of d ...
and then dispersing the insects over target areas.An Introduction to Biological Weapons, Their Prohibition, and the Relationship to Biosafety
", '' The Sunshine Project'', April 2002. Retrieved 25 December 2008.
The insects then act as a
vector Vector most often refers to: * Euclidean vector, a quantity with a magnitude and a direction * Disease vector, an agent that carries and transmits an infectious pathogen into another living organism Vector may also refer to: Mathematics a ...
, infecting any person or animal they might bite. Another type of EW is a direct insect attack against crops; the insect may not be infected with any pathogen but instead represents a threat to agriculture. The final method uses uninfected insects, such as bees or wasps, to directly attack the enemy.


Genetics

Theoretically, novel approaches in biotechnology, such as synthetic biology could be used in the future to design novel types of biological warfare agents. # Would demonstrate how to render a vaccine ineffective; # Would confer resistance to therapeutically useful antibiotics or antiviral agents; # Would enhance the virulence of a pathogen or render a nonpathogen virulent; # Would increase the transmissibility of a pathogen; # Would alter the host range of a pathogen; # Would enable the evasion of diagnostic/detection tools; # Would enable the weaponization of a biological agent or toxin. Most of the biosecurity concerns in synthetic biology are focused on the role of DNA synthesis and the risk of producing genetic material of lethal viruses (e.g. 1918 Spanish flu, polio) in the lab. Recently, the CRISPR/Cas system has emerged as a promising technique for gene editing. It was hailed by The Washington Post as "the most important innovation in the synthetic biology space in nearly 30 years." While other methods take months or years to edit gene sequences, CRISPR speeds that time up to weeks. Due to its ease of use and accessibility, it has raised a number of ethical concerns, especially surrounding its use in the biohacking space.


By target


Anti-personnel

Ideal characteristics of a biological agent to be used as a weapon against humans are high
infectivity In epidemiology, infectivity is the ability of a pathogen In biology, a pathogen (, "suffering", "passion" and , "producer of"), in the oldest and broadest sense, is any organism or agent that can produce disease. A pathogen may also be refer ...
, high
virulence Virulence is a pathogen's or microorganism's ability to cause damage to a host. In most cases, especially in animal systems, virulence refers to the degree of damage caused by a microbe to its host. The pathogenicity of an organism—its abili ...
, non-availability of
vaccine A vaccine is a biological Dosage form, preparation that provides active acquired immunity to a particular infectious disease, infectious or cancer, malignant disease. The safety and effectiveness of vaccines has been widely studied and verifi ...
s and availability of an effective and efficient delivery system. Stability of the weaponized agent (the ability of the agent to retain its infectivity and virulence after a prolonged period of storage) may also be desirable, particularly for military applications, and the ease of creating one is often considered. Control of the spread of the agent may be another desired characteristic. The primary difficulty is not the production of the biological agent, as many biological agents used in weapons can be manufactured relatively quickly, cheaply and easily. Rather, it is the weaponization, storage, and delivery in an effective vehicle to a vulnerable target that pose significant problems. For example, ''
Bacillus anthracis ''Bacillus anthracis'' is a gram-positive and rod-shaped bacterium that causes anthrax, a deadly disease to livestock and, occasionally, to humans. It is the only permanent (obligate) pathogen within the genus ''Bacillus''. Its infection is a ty ...
'' is considered an effective agent for several reasons. First, it forms hardy
spore In biology, a spore is a unit of sexual reproduction, sexual (in fungi) or asexual reproduction that may be adapted for biological dispersal, dispersal and for survival, often for extended periods of time, in unfavourable conditions. Spores fo ...
s, perfect for dispersal aerosols. Second, this organism is not considered transmissible from person to person, and thus rarely if ever causes secondary infections. A pulmonary anthrax infection starts with ordinary
influenza Influenza, commonly known as the flu, is an infectious disease caused by influenza viruses. Symptoms range from mild to severe and often include fever, runny nose, sore throat, muscle pain, headache, coughing, and fatigue. These sympto ...
-like symptoms and progresses to a lethal hemorrhagic mediastinitis within 3–7 days, with a fatality rate that is 90% or higher in untreated patients. Finally, friendly personnel and civilians can be protected with suitable
antibiotic An antibiotic is a type of antimicrobial substance active against bacteria. It is the most important type of antibacterial agent for fighting pathogenic bacteria, bacterial infections, and antibiotic medications are widely used in the therapy ...
s. Agents considered for weaponization, or known to be weaponized, include bacteria such as ''Bacillus anthracis'', ''
Brucella ''Brucella'' is a genus of Gram-negative bacterium, bacteria, named after David Bruce (microbiologist), David Bruce (1855–1931). They are small (0.5 to 0.7 by 0.6 to 1.5 μm), non-Bacterial capsule, encapsulated, non-motile, facultatively ...
'' spp., ''
Burkholderia mallei ''Burkholderia mallei'' is a Gram-negative, bipolar, aerobic bacterium, a human and animal pathogen of genus '' Burkholderia'' causing glanders; the Latin name of this disease (''malleus'') gave its name to the species causing it. It is closely ...
'', '' Burkholderia pseudomallei'', '' Chlamydophila psittaci'', ''
Coxiella burnetii ''Coxiella burnetii'' is an obligate intracellular bacterial pathogen, and is the causative agent of Q fever. The genus ''Coxiella'' is morphologically similar to '' Rickettsia'', but with a variety of physiological differences genetically cla ...
'', ''
Francisella tularensis ''Francisella tularensis'' is a pathogenic species of Gram-negative coccobacillus, an aerobic bacterium. It is nonspore-forming, nonmotile, and the causative agent of tularemia, the pneumonic form of which is often lethal without treatment. It i ...
'', some of the Rickettsiaceae (especially '' Rickettsia prowazekii'' and '' Rickettsia rickettsii''), ''
Shigella ''Shigella'' is a genus of bacteria that is Gram negative, facultatively anaerobic, non–spore-forming, nonmotile, rod shaped, and is genetically nested within ''Escherichia''. The genus is named after Kiyoshi Shiga, who discovered it in 1 ...
'' spp., ''
Vibrio cholerae ''Vibrio cholerae'' is a species of Gram-negative bacteria, Gram-negative, Facultative anaerobic organism, facultative anaerobe and Vibrio, comma-shaped bacteria. The bacteria naturally live in Brackish water, brackish or saltwater where they att ...
'', and ''
Yersinia pestis ''Yersinia pestis'' (''Y. pestis''; formerly ''Pasteurella pestis'') is a Gram-negative bacteria, gram-negative, non-motile bacteria, non-motile, coccobacillus Bacteria, bacterium without Endospore, spores. It is related to pathogens ''Yer ...
''. Many viral agents have been studied and weaponized, including some of the
Bunyaviridae ''Bunyaviricetes'' is a class of segmented negative-strand RNA viruses with mainly tripartite genomes. Member viruses infect arthropods, plants, protozoans, and vertebrates. The name ''Bunyaviricetes'' derives from Bunyamwera, where the virus B ...
(especially Rift Valley fever virus),
Ebola Ebola, also known as Ebola virus disease (EVD) and Ebola hemorrhagic fever (EHF), is a viral hemorrhagic fever in humans and other primates, caused by ebolaviruses. Symptoms typically start anywhere between two days and three weeks after in ...
virus, many of the
Flaviviridae ''Flaviviridae'' is a family of Viral envelope, enveloped positive-strand RNA viruses which mainly infect mammals and birds. They are primarily spread through arthropod vector (epidemiology), vectors (mainly ticks and mosquitoes). The family gets ...
(especially Japanese encephalitis virus), Machupo virus,
Coronaviruses Coronaviruses are a group of related RNA viruses that cause diseases in mammals and birds. In humans and birds, they cause respiratory tract infections that can range from mild to lethal. Mild illnesses in humans include some cases of the comm ...
,
Marburg virus Marburg virus (MARV) is a hemorrhagic fever virus of the '' Filoviridae'' family of viruses and a member of the species '' Marburg marburgvirus'', genus '' Marburgvirus''. It causes Marburg virus disease in primates, a form of viral hemorrhag ...
, Variola virus, and yellow fever virus. Fungal agents that have been studied include '' Coccidioides'' spp. Toxins that can be used as weapons include
ricin Ricin ( ) is a lectin (a carbohydrate-binding protein) and a highly potent toxin produced in the seeds of the castor oil plant, ''Ricinus communis''. The median lethal dose (LD50) of ricin for mice is around 22 micrograms per kilogram of body ...
, staphylococcal enterotoxin B,
botulinum toxin Botulinum toxin, or botulinum neurotoxin (commonly called botox), is a neurotoxic protein produced by the bacterium ''Clostridium botulinum'' and related species. It prevents the release of the neurotransmitter acetylcholine from axon en ...
,
saxitoxin Saxitoxin (STX) is a potent neurotoxin and the best-known paralytic shellfish toxin. Ingestion of saxitoxin by humans, usually by consumption of shellfish contaminated by toxic algal blooms, is responsible for the illness known as paralytic she ...
, and many
mycotoxin A mycotoxin (from the Greek μύκης , "fungus" and τοξικός , "poisonous") is a toxic secondary metabolite produced by fungi and is capable of causing disease and death in both humans and other animals. The term 'mycotoxin' is usually rese ...
s. These toxins and the organisms that produce them are sometimes referred to as select agents. In the United States, their possession, use, and transfer are regulated by the
Centers for Disease Control and Prevention The Centers for Disease Control and Prevention (CDC) is the National public health institutes, national public health agency of the United States. It is a Federal agencies of the United States, United States federal agency under the United S ...
's Select Agent Program. The former US biological warfare program categorized its weaponized anti-personnel bio-agents as either Lethal Agents (''Bacillus anthracis'', ''Francisella tularensis'', Botulinum toxin) or Incapacitating Agents (''Brucella suis'', ''Coxiella burnetii'', Venezuelan equine encephalitis virus, Staphylococcal enterotoxin B).


Anti-agriculture


Anti-crop/anti-vegetation/anti-fisheries

The United States developed an anti-crop capability during the
Cold War The Cold War was a period of global Geopolitics, geopolitical rivalry between the United States (US) and the Soviet Union (USSR) and their respective allies, the capitalist Western Bloc and communist Eastern Bloc, which lasted from 1947 unt ...
that used plant diseases ( bioherbicides, or mycoherbicides) for destroying enemy agriculture. Biological weapons also target fisheries as well as water-based vegetation. It was believed that the destruction of enemy agriculture on a strategic scale could thwart Sino-Soviet aggression in a general war. Diseases such as wheat blast and
rice blast ''Magnaporthe grisea'', also known as rice blast fungus, rice rotten neck, rice seedling blight, blast of rice, oval leaf spot of graminea, pitting disease, ryegrass blast, Johnson spot, neck blast, wheat blast and , is a plant-pathogenic fungus ...
were weaponized in aerial spray tanks and cluster bombs for delivery to enemy watersheds in agricultural regions to initiate epiphytotic (epidemics among plants). On the other hand, some sources report that these agents were ''stockpiled'' but never ''weaponized''. When the United States renounced its offensive biological warfare program in 1969 and 1970, the vast majority of its biological arsenal was composed of these plant diseases. Enterotoxins and Mycotoxins were not affected by Nixon's order. Though herbicides are chemicals, they are often grouped with biological warfare and chemical warfare because they may work in a similar manner as biotoxins or bioregulators. The Army Biological Laboratory tested each agent and the Army's Technical Escort Unit was responsible for the transport of all chemical, biological, radiological (nuclear) materials. Biological warfare can also specifically target plants to destroy crops or defoliate vegetation. The United States and Britain discovered plant growth regulators (i.e.,
herbicide Herbicides (, ), also commonly known as weed killers, are substances used to control undesired plants, also known as weeds.EPA. February 201Pesticides Industry. Sales and Usage 2006 and 2007: Market Estimates. Summary in press releasMain page f ...
s) during the Second World War, which were then used by the UK in the counterinsurgency operations of the
Malayan Emergency The Malayan Emergency, also known as the Anti–British National Liberation War, was a guerrilla warfare, guerrilla war fought in Federation of Malaya, Malaya between communist pro-independence fighters of the Malayan National Liberation Arm ...
. Inspired by the use in Malaysia, the US military effort in the
Vietnam War The Vietnam War (1 November 1955 – 30 April 1975) was an armed conflict in Vietnam, Laos, and Cambodia fought between North Vietnam (Democratic Republic of Vietnam) and South Vietnam (Republic of Vietnam) and their allies. North Vietnam w ...
included a mass dispersal of a variety of herbicides, famously
Agent Orange Agent Orange is a chemical herbicide and defoliant, one of the tactical uses of Rainbow Herbicides. It was used by the U.S. military as part of its herbicidal warfare program, Operation Ranch Hand, during the Vietnam War from 1962 to 1971. T ...
, with the aim of destroying farmland and defoliating forests used as cover by the
Viet Cong The Viet Cong (VC) was an epithet and umbrella term to refer to the communist-driven armed movement and united front organization in South Vietnam. It was formally organized as and led by the National Liberation Front of South Vietnam, and ...
. Sri Lanka deployed military defoliants in its prosecution of the Eelam War against Tamil insurgents.


Anti-livestock

During World War I, German saboteurs used
anthrax Anthrax is an infection caused by the bacterium '' Bacillus anthracis'' or ''Bacillus cereus'' biovar ''anthracis''. Infection typically occurs by contact with the skin, inhalation, or intestinal absorption. Symptom onset occurs between one ...
and
glanders Glanders is a contagious, zoonotic infectious disease caused by the bacterium '' Burkholderia mallei'', which primarily occurs in horses, mules, and donkeys, but can also be contracted by dogs and cats, pigs, goats, and humans. The term ''glan ...
to sicken cavalry horses in US and France, sheep in Romania, and livestock in Argentina intended for the Entente forces. One of these German saboteurs was
Anton Dilger Anton Casimir Dilger (13 February 1884 – 17 October 1918) was a German-American medical doctor and a main actor in the German biological warfare sabotage program during World War I. Dilger eventually fled to Madrid, Spain, where he died durin ...
. Also, Germany itself became a victim of similar attacks – horses bound for Germany were infected with Burkholderia by French operatives in Switzerland. During World War II, the US and Canada secretly investigated the use of
rinderpest Rinderpest (also cattle plague or steppe murrain) was an infectious viral disease of cattle, domestic water buffalo, and many other species of even-toed ungulates, including gaurs, African Buffalo, buffaloes, large antelope, deer, giraffes, wilde ...
, a highly lethal disease of cattle, as a bioweapon. In the 1980s Soviet Ministry of Agriculture had successfully developed variants of
foot-and-mouth disease Foot-and-mouth disease (FMD) or hoof-and-mouth disease (HMD) is an infectious disease, infectious and sometimes fatal virus (biology), viral disease that primarily affects even-toed ungulates, including domestic and wild Bovidae, bovids. The vir ...
, and
rinderpest Rinderpest (also cattle plague or steppe murrain) was an infectious viral disease of cattle, domestic water buffalo, and many other species of even-toed ungulates, including gaurs, African Buffalo, buffaloes, large antelope, deer, giraffes, wilde ...
against cows, African swine fever for pigs, and psittacosis for chickens. These agents were prepared to spray them down from tanks attached to airplanes over hundreds of miles. The secret program was code-named "Ecology". During the
Mau Mau Uprising The Mau Mau rebellion (1952–1960), also known as the Mau Mau uprising, Mau Mau revolt, or Kenya Emergency, was a war in the British Kenya Colony (1920–1963) between the Kenya Land and Freedom Army (KLFA), also known as the Mau Mau, and the ...
in 1952, the poisonous
latex Latex is an emulsion (stable dispersion) of polymer microparticles in water. Latices are found in nature, but synthetic latices are common as well. In nature, latex is found as a wikt:milky, milky fluid, which is present in 10% of all floweri ...
of the African milk bush was used to kill cattle.


Defensive operations


Medical countermeasures

In 2010 at The Meeting of the States Parties to the Convention on the Prohibition of the Development, Production and Stockpiling of Bacteriological (Biological) and Toxin Weapons and Their Destruction in
Geneva Geneva ( , ; ) ; ; . is the List of cities in Switzerland, second-most populous city in Switzerland and the most populous in French-speaking Romandy. Situated in the southwest of the country, where the Rhône exits Lake Geneva, it is the ca ...
the sanitary epidemiological reconnaissance was suggested as well-tested means for enhancing the monitoring of infections and parasitic agents, for the practical implementation of the
International Health Regulations The International Health Regulations (IHR), first adopted by the World Health Assembly in 1969 and last revised in 2005, are legally binding rules that only apply to the WHO that is an instrument that aims for international collaboration "to prev ...
(2005). The aim was to prevent and minimize the consequences of natural outbreaks of dangerous infectious diseases as well as the threat of alleged use of biological weapons against BTWC States Parties. Many countries require their active-duty
military A military, also known collectively as armed forces, is a heavily armed, highly organized force primarily intended for warfare. Militaries are typically authorized and maintained by a sovereign state, with their members identifiable by a d ...
personnel to get vaccinated for certain diseases that may potentially be used as a bioweapon such as anthrax, smallpox, and various other vaccines depending on the Area of Operations of the individual military units and commands.


Public health and disease surveillance

Most classical and modern biological weapons' pathogens can be obtained from a plant or an animal which is naturally infected. In the largest biological weapons accident known—the anthrax outbreak in Sverdlovsk (now
Yekaterinburg Yekaterinburg (, ; ), alternatively Romanization of Russian, romanized as Ekaterinburg and formerly known as Sverdlovsk ( ; 1924–1991), is a city and the administrative centre of Sverdlovsk Oblast and the Ural Federal District, Russia. The ci ...
) in the
Soviet Union The Union of Soviet Socialist Republics. (USSR), commonly known as the Soviet Union, was a List of former transcontinental countries#Since 1700, transcontinental country that spanned much of Eurasia from 1922 until Dissolution of the Soviet ...
in 1979—sheep became ill with anthrax as far as from the release point of the organism from a military facility in the southeastern portion of the city and still off-limits to visitors today, (see Sverdlovsk Anthrax leak). Thus, a robust surveillance system involving human clinicians and veterinarians may identify a bioweapons attack early in the course of an epidemic, permitting the prophylaxis of disease in the vast majority of people (and animals) exposed but not yet ill. For example, in the case of anthrax, it is likely that by 24–36 hours after an attack, some small percentage of individuals (those with the compromised immune system or who had received a large dose of the organism due to proximity to the release point) will become ill with classical symptoms and signs (including a virtually unique
chest X-ray A chest radiograph, chest X-ray (CXR), or chest film is a Projectional radiography, projection radiograph of the chest used to diagnose conditions affecting the chest, its contents, and nearby structures. Chest radiographs are the most common fi ...
finding, often recognized by public health officials if they receive timely reports). The incubation period for humans is estimated to be about 11.8 days to 12.1 days. This suggested period is the first model that is independently consistent with data from the largest known human outbreak. These projections refine previous estimates of the distribution of early-onset cases after a release and support a recommended 60-day course of prophylactic antibiotic treatment for individuals exposed to low doses of anthrax. By making these data available to local public health officials in real time, most models of anthrax epidemics indicate that more than 80% of an exposed population can receive antibiotic treatment before becoming symptomatic, and thus avoid the moderately high mortality of the disease.


Common epidemiological warnings

From most specific to least specific: #Single cause of a certain disease caused by an uncommon agent, with lack of an epidemiological explanation. #Unusual, rare, genetically engineered strain of an agent. #High morbidity and mortality rates in regards to patients with the same or similar symptoms. #Unusual presentation of the disease. #Unusual geographic or seasonal distribution. #Stable endemic disease, but with an unexplained increase in relevance. #Rare transmission (aerosols, food, water). #No illness presented in people who were/are not exposed to "common ventilation systems (have separate closed ventilation systems) when illness is seen in persons in close proximity who have a common ventilation system." #Different and unexplained diseases coexisting in the same patient without any other explanation. #Rare illness that affects a large, disparate population (respiratory disease might suggest the pathogen or agent was inhaled). #Illness is unusual for a certain population or age-group in which it takes presence. #Unusual trends of death and illness in animal populations, previous to or accompanying illness in humans. #Many affected reaching out for treatment at the same time. #Similar genetic makeup of agents in affected individuals. #Simultaneous collections of similar illness in non-contiguous areas, domestic, or foreign. #An abundance of cases of unexplained diseases and deaths.


Bioweapon identification

The goal of
biodefense Biodefense refers to measures to counter biological agent, biological threats, reduce biological risks, and prepare for, respond to, and recover from bioincidents, whether naturally occurring, accidental, or deliberate in origin and whether impa ...
is to integrate the sustained efforts of the national and homeland security, medical, public health, intelligence, diplomatic, and law enforcement communities. Health care providers and public health officers are among the first lines of defense. In some countries private, local, and provincial (state) capabilities are being augmented by and coordinated with federal assets, to provide layered defenses against biological weapon attacks. During the first Gulf War the United Nations activated a biological and chemical response team, Task Force Scorpio, to respond to any potential use of weapons of mass destruction on civilians. The traditional approach toward protecting agriculture, food, and water: focusing on the natural or unintentional introduction of a disease is being strengthened by focused efforts to address current and anticipated future biological weapons threats that may be deliberate, multiple, and repetitive. The growing threat of biowarfare agents and bioterrorism has led to the development of specific field tools that perform on-the-spot analysis and identification of encountered suspect materials. One such technology, being developed by researchers from the
Lawrence Livermore National Laboratory Lawrence Livermore National Laboratory (LLNL) is a Federally funded research and development centers, federally funded research and development center in Livermore, California, United States. Originally established in 1952, the laboratory now i ...
(LLNL), employs a "sandwich immunoassay", in which fluorescent dye-labeled antibodies aimed at specific
pathogen In biology, a pathogen (, "suffering", "passion" and , "producer of"), in the oldest and broadest sense, is any organism or agent that can produce disease. A pathogen may also be referred to as an infectious agent, or simply a Germ theory of d ...
s are attached to silver and gold nanowires. In the
Netherlands , Terminology of the Low Countries, informally Holland, is a country in Northwestern Europe, with Caribbean Netherlands, overseas territories in the Caribbean. It is the largest of the four constituent countries of the Kingdom of the Nether ...
, the company TNO has designed Bioaerosol Single Particle Recognition eQuipment (BiosparQ). This system would be implemented into the national response plan for bioweapon attacks in the Netherlands. Researchers at Ben Gurion University in Israel are developing a different device called the BioPen, essentially a "Lab-in-a-Pen", which can detect known biological agents in under 20 minutes using an adaptation of the
ELISA The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay is a solid-phase type of enzyme immunoassay (EIA) to detect the presence of ...
, a similar widely employed immunological technique, that in this case incorporates fiber optics.


List of programs, projects and sites by country


United States

* Fort Detrick, Maryland ** US Army Biological Warfare Laboratories (1943–69) *** Building 470 *** One-Million-Liter Test Sphere *** Operation Sea-Spray *** Operation Whitecoat (1954–73) ** US entomological warfare program *** Operation Big Itch *** Operation Big Buzz *** Operation Drop Kick *** Operation May Day * Project Bacchus * Project Clear Vision * Project SHAD * Project 112 * Horn Island Testing Station * Fort Terry * Granite Peak Installation * Vigo Ordnance Plant


United Kingdom

* Porton Down * Gruinard Island * Nancekuke * Operation Vegetarian (1942–1944) *Open-air field tests: ** Operation Harness off
Antigua Antigua ( ; ), also known as Waladli or Wadadli by the local population, is an island in the Lesser Antilles. It is one of the Leeward Islands in the Caribbean region and the most populous island of the country of Antigua and Barbuda. Antigua ...
, 1948–1950. ** Operation Cauldron off
Stornoway Stornoway (; ) is the main town, and by far the largest, of the Outer Hebrides (or Western Isles), and the capital of Lewis and Harris in Scotland. The town's population is around 6,953, making it the third-largest island town in Scotlan ...
, 1952. ** Operation Hesperus off
Stornoway Stornoway (; ) is the main town, and by far the largest, of the Outer Hebrides (or Western Isles), and the capital of Lewis and Harris in Scotland. The town's population is around 6,953, making it the third-largest island town in Scotlan ...
, 1953. ** Operation Ozone off Nassau, 1954. ** Operation Negation off Nassau, 1954–5.


Soviet Union and Russia

* Biopreparat (18 labs and production centers) ** Stepnogorsk Scientific and Technical Institute for Microbiology, Stepnogorsk, northern
Kazakhstan Kazakhstan, officially the Republic of Kazakhstan, is a landlocked country primarily in Central Asia, with a European Kazakhstan, small portion in Eastern Europe. It borders Russia to the Kazakhstan–Russia border, north and west, China to th ...
** Institute of Ultra Pure Biochemical Preparations,
Leningrad Saint Petersburg, formerly known as Petrograd and later Leningrad, is the List of cities and towns in Russia by population, second-largest city in Russia after Moscow. It is situated on the Neva, River Neva, at the head of the Gulf of Finland ...
, a weaponized plague center ** Vector State Research Center of Virology and Biotechnology (VECTOR), a weaponized smallpox center ** Institute of Applied Biochemistry, Omutninsk ** Kirov bioweapons production facility,
Kirov, Kirov Oblast Kirov (, ), formerly known as Vyatka ( rus, Вя́тка, links=no, a=, p= �vʲatka until 1934 and as Khlynov () from 1457 to 1780, is the largest types of inhabited localities in Russia, city and administrative center of Kirov Oblast, Russia ...
** Zagorsk smallpox production facility, Zagorsk ** Berdsk bioweapons production facility, Berdsk ** Bioweapons research facility, Obolensk ** Sverdlovsk bioweapons production facility (Military Compound 19), Sverdlovsk, a weaponized anthrax center * Institute of Virus Preparations * Poison laboratory of the Soviet secret services *
Vozrozhdeniya Vozrozhdeniya Island (; ; ) was an List of islands in lakes, island in the Aral Sea. The former island's territory is split between Uzbekistan and Kazakhstan. In 1954, the Soviet Union constructed a biological weapons test site called Aralsk-7 ...
* Project Bonfire * Project Factor


Japan

*
Unit 731 , short for Manchu Detachment 731 and also known as the Kamo Detachment and the Ishii Unit, was a covert biological and chemical warfare research and development unit of the Imperial Japanese Army that engaged in lethal human experimentat ...
* Zhongma Fortress * Kaimingjie germ weapon attack * Khabarovsk War Crime Trials * Epidemic Prevention and Water Purification Department


Iraq

* Al Hakum * Salman Pak facility * Al Manal facility


South Africa

* Project Coast * Delta G Scientific Company * Roodeplaat Research Laboratories * Protechnik


Rhodesia


Canada

*
Grosse Isle, Quebec Grosse Isle (, , "big island") is an island located in the St. Lawrence River in Quebec, Canada. It is one of the islands of the 21-island Isle-aux-Grues archipelago. It is part of the municipality of Saint-Antoine-de-l'Isle-aux-Grues, locate ...
, site (1939–45) of research into anthrax and other agents * DRDC Suffield, Suffield, Alberta


List of associated people

''Bioweaponeers:'' :''Includes scientists and administrators'' * Shyh-Ching Lo * Kanatjan Alibekov, known as Ken Alibek * Ira Baldwin * Wouter Basson * Kurt Blome *Eugen von Haagen *
Anton Dilger Anton Casimir Dilger (13 February 1884 – 17 October 1918) was a German-American medical doctor and a main actor in the German biological warfare sabotage program during World War I. Dilger eventually fled to Madrid, Spain, where he died durin ...
* Paul Fildes * Arthur Galston (unwittingly) *Kurt Gutzeit * Riley D. Housewright *
Shirō Ishii Surgeon General was a Japanese microbiologist and Military medicine, army medical officer, who served as the director of Unit 731, a biological warfare unit of the Imperial Japanese Army. Ishii led the development and application of biological ...
* Elvin A. Kabat * George W. Merck * Frank Olson * Vladimir Pasechnik * William C. Patrick III * Sergei Popov * Theodor Rosebury * Rihab Rashid Taha * Prince Tsuneyoshi Takeda * Huda Salih Mahdi Ammash * Nassir al-Hindawi * Erich Traub * Auguste Trillat * Baron Otto von Rosen * Yujiro Wakamatsu * Yazid Sufaat ''Writers and activists:'' * Jack Trudel * Daniel Barenblatt * Leonard A. Cole *Stephen Endicott * Arthur Galston * Jeanne Guillemin * Edward Hagerman * Sheldon H. Harris *
Nicholas D. Kristof Nicholas Donabet Kristof (born April 27, 1959) is an American journalist and political commentator. A winner of two Pulitzer Prizes, he is a regular CNN contributor and an op-ed columnist for ''The New York Times''. Born in Chicago, Kristof was ...
*
Joshua Lederberg Joshua Lederberg (May 23, 1925 – February 2, 2008) was an American molecular biology, molecular biologist known for his work in microbial genetics, artificial intelligence, and the United States space program. He was 33 years old when he won t ...
* Matthew Meselson * Toby Ord *
Richard Preston Richard Preston (born August 5, 1954) is a writer for ''The New Yorker'' and bestselling author who has written books about infectious disease, bioterrorism, redwoods and other subjects, as well as fiction. Biography Preston was born in Cambr ...
* Ed Regis * Mark Wheelis * David Willman * Aaron Henderson


In popular culture


See also

* Animal-borne bomb attacks *
Antibiotic resistance Antimicrobial resistance (AMR or AR) occurs when microbes evolve mechanisms that protect them from antimicrobials, which are drugs used to treat infections. This resistance affects all classes of microbes, including bacteria (antibiotic resis ...
*
Asymmetric warfare Asymmetric warfare (or asymmetric engagement) is a type of war between belligerents whose relative military power, strategy or tactics differ significantly. This type of warfare often, but not necessarily, involves insurgents, terrorist grou ...
*
Baker Island Baker Island, once known as New Nantucket in the early 19th century, is a small, uninhabited atoll located just north of the Equator in the central Pacific Ocean, approximately southwest of Honolulu. Positioned almost halfway between Hawaii a ...
*
Bioaerosol Bioaerosols (short for biological aerosols) are a subcategory of particles released from terrestrial and marine ecosystems into the atmosphere. They consist of both living and non-living components, such as fungi, pollen, bacteria and viruses. Comm ...
*
Biological contamination Biology is the scientific study of life and living organisms. It is a broad natural science that encompasses a wide range of fields and unifying principles that explain the structure, function, growth, origin, evolution, and distribution o ...
*
Biological pest control Biological control or biocontrol is a method of controlling pests, whether pest animals such as insects and mites, weeds, or pathogens affecting animals or plants by using other organisms. It relies on predation, parasitism, herbivory, or ot ...
*
Biosecurity Biosecurity refers to measures aimed at preventing the introduction or spread of harmful organisms (e.g. viruses, bacteria, plants, animals etc.) intentionally or unintentionally outside their native range or within new environments. In agricult ...
*
Chemical weapon A chemical weapon (CW) is a specialized munition that uses chemicals formulated to inflict death or harm on humans. According to the Organisation for the Prohibition of Chemical Weapons (OPCW), this can be any chemical compound intended as ...
*
Counterinsurgency Counterinsurgency (COIN, or NATO spelling counter-insurgency) is "the totality of actions aimed at defeating irregular forces". The Oxford English Dictionary defines counterinsurgency as any "military or political action taken against the ac ...
* Discredited AIDS origins theories * Enterotoxin *
Entomological warfare Entomological warfare (EW) is a type of biological warfare that uses insects to interrupt supply lines by damaging crops, or to directly harm enemy combatants and civilian populations. There have been several programs which have attempted to instit ...
* Ethnic bioweapon *
Herbicidal warfare Herbicidal warfare is the use of substances primarily designed to destroy the plant-based ecosystem of an area. Although herbicidal warfare use chemical substances, its main purpose is to disrupt agricultural food production and/or to destroy p ...
* Hittite plague *
Human experimentation in the United States Numerous human subject research, experiments which were performed on human test subjects in the United States in the past are now considered to have been Unethical human experimentation, unethical, because they were performed without the knowled ...
* John W. Powell * Johnston Atoll Chemical Agent Disposal System * List of CBRN warfare forces * McNeill's law * Military animal *
Mycotoxin A mycotoxin (from the Greek μύκης , "fungus" and τοξικός , "poisonous") is a toxic secondary metabolite produced by fungi and is capable of causing disease and death in both humans and other animals. The term 'mycotoxin' is usually rese ...
* Plum Island Animal Disease Center * Project 112 * Project AGILE * Project SHAD * Rhodesia and weapons of mass destruction *
Trichothecene Trichothecenes constitute a large group of chemically related mycotoxins. They are produced by Fungus, fungi of the genera ''Fusarium'', ''Myrothecium'', ''Trichoderma'', ''Podostroma'', ''Trichothecium'', ''Cephalosporium'', ', ''Stachybotrys'' ...
*
Well poisoning Well poisoning is the act of malicious manipulation of potable water resources in order to cause illness or death, or to deny an opponent access to fresh water resources. Well poisoning has been historically documented as a strategy during war ...
* Yellow rain


References


Further reading

* * Counterproliferation Paper No. 53, USAF Counterproliferation Center, Air University, Maxwell Air Force Base, Alabama, USA. * * * * * * * * * * * * * * * * * * * * * * * * * * * *


External links


Biological weapons and international humanitarian law
, ICRC
WHO: Health Aspects of Biological and Chemical Weapons
*
USAMRIID
()—U.S. Army Medical Research Institute of Infectious Diseases {{DEFAULTSORT:Biological Warfare Bioethics Warfare by type