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Middlebrook 7H11 Agar
Middlebrook 7H11 agar is identical to Middlebrook 7H10 Agar, Middlebrook 7H10 agar, with an addition of pancreatic digest of casein to facilitate the growth of fastidious cultures of ''M. tuberculosis''. Mycobactin, Mycobactin J may also be added to Middlebrook 7H11 agar to allow the recovery of ''M. genavense''. See also * Lowenstein-Jensen medium * Middlebrook 7H9 Broth, Middlebrook 7H9 broth References External links Middlebrook 7H11 Agar {{DEFAULTSORT:Middlebrook 7h11 Agar Microbiological media ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Middlebrook 7H10 Agar
Middlebrook 7H10 Agar is a solid growth medium specially used for culture of ''Mycobacterium'', notably '' Mycobacterium tuberculosis''. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria. Composition Ingredients (g/L) * Ammonium sulfate, 0.50 * Monopotassium phosphate, 1.50 * Disodium phosphate, 1.50 * Sodium citrate, 0.40 * Magnesium sulfate, 0.025 * Zinc sulfate, 0.001 * Copper sulfate, 0.001 * L-Glutamic acid, 0.50 * Ferric ammonium citrate, 0.04 * Pyridoxine hydrochloride, 0.001 * Biotin, 0.0005 * Malachite green, 0.00025 * Agar, 15.00 * Calcium chloride, 0.0005 Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks. See also * Lowenstein-Jensen medium * Middlebrook 7H9 Broth Middlebrook 7H9 broth is a liquid growth medium specially used for culture of'' Mycobacterium'' species, notably ''Mycobacterium tuberculosis ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pancreatic
The pancreas is an organ of the digestive system and endocrine system of vertebrates. In humans, it is located in the abdomen behind the stomach and functions as a gland. The pancreas is a mixed or heterocrine gland, i.e. it has both an endocrine and a digestive exocrine function. 99% of the pancreas is exocrine and 1% is endocrine. As an endocrine gland, it functions mostly to regulate blood sugar levels, secreting the hormones insulin, glucagon, somatostatin, and pancreatic polypeptide. As a part of the digestive system, it functions as an exocrine gland secreting pancreatic juice into the duodenum through the pancreatic duct. This juice contains bicarbonate, which neutralizes acid entering the duodenum from the stomach; and digestive enzymes, which break down carbohydrates, proteins, and fats in food entering the duodenum from the stomach. Inflammation of the pancreas is known as pancreatitis, with common causes including chronic alcohol use and gallstones. Because of it ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Casein
Casein ( , from Latin ''caseus'' "cheese") is a family of related phosphoproteins ( αS1, aS2, β, κ) that are commonly found in mammalian milk, comprising about 80% of the proteins in cow's milk and between 20% and 60% of the proteins in human milk. Sheep and buffalo milk have a higher casein content than other types of milk with human milk having a particularly low casein content. Casein has a wide variety of uses, from being a major component of cheese, to use as a food additive. The most common form of casein is sodium caseinate. In milk, casein undergoes phase separation to form colloidal casein micelles, a type of secreted biomolecular condensate. As a food source, casein supplies amino acids, carbohydrates, and two essential elements, calcium and phosphorus. Composition Casein contains a high number of proline amino acids which hinder the formation of common secondary structural motifs of proteins. There are also no disulfide bridges. As a result, it has relatively ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mycobactin
Mycobactin is a siderophore used by members of the genus '' Mycobacterium'' to shuttle free extracellular iron ions into the cytoplasm of mycobacterial cells. The pathogen ''M. tuberculosis'' can synthesize its own mycobactin for this purpose, however there are other mycobacteria such as ''M. avium'' subspecies ''paratuberculosis Paratuberculosis is a contagious, chronic and sometimes fatal infection that primarily affects the small intestine of ruminants. It is caused by the bacterium ''Mycobacterium avium'' subspecies ''paratuberculosis''. Infections normally affect ru ...'' that cannot produce this siderophore and thus it must be supplied to cultivate this organism in the lab. References {{Reflist Siderophores ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Middlebrook 7H9 Broth
Middlebrook 7H9 broth is a liquid growth medium specially used for culture of'' Mycobacterium'' species, notably ''Mycobacterium tuberculosis ''Mycobacterium tuberculosis'' (M. tb) is a species of pathogenic bacteria in the family Mycobacteriaceae and the causative agent of tuberculosis. First discovered in 1882 by Robert Koch, ''M. tuberculosis'' has an unusual, waxy coating on its c ...''. Composition * Ammonium sulfate * L-Glutamic acid * Sodium citrate * Pyridoxine * Biotin * Disodium phosphate * Monopotassium phosphate * Ferric ammonium citrate * Magnesium sulfate * Calcium chloride * Zinc sulfate * Copper Sulfate Middlebrook 7H9 broth supports the growth of mycobacterial species when supplemented with nutrients such as glycerol, oleic acid, albumin, and dextrose, except for '' Mycobacterium bovis'', which is inhibited by glycerol. Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks. Middlebrook broth is commonly used ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
