Middlebrook 7H9 broth is a liquid growth medium specially used for culture of'' Mycobacterium'' species, notably ''
Mycobacterium tuberculosis
''Mycobacterium tuberculosis'' (M. tb), also known as Koch's bacillus, is a species of pathogenic bacteria in the family Mycobacteriaceae and the causative agent of tuberculosis.
First discovered in 1882 by Robert Koch, ''M. tuberculosis'' ha ...
''.
Composition
*
Ammonium sulfate
Ammonium sulfate (American English and international scientific usage; ammonium sulphate in British English); (NH4)2SO4, is an inorganic salt with a number of commercial uses. The most common use is as a soil fertilizer. It contains 21% nitrogen a ...
*
L-Glutamic acid
*
Sodium citrate Sodium citrate may refer to any of the sodium salts of citric acid (though most commonly the third):
* Monosodium citrate
* Disodium citrate
* Trisodium citrate
The three forms of salt are collectively known by the E number E331.
Applications
...
*
Pyridoxine
Pyridoxine (PN) is a form of vitamin B6 found commonly in food and used as a dietary supplement. As a supplement it is used to treat and prevent pyridoxine deficiency, sideroblastic anaemia, pyridoxine-dependent epilepsy, certain metaboli ...
*
Biotin
Biotin (also known as vitamin B7 or vitamin H) is one of the B vitamins. It is involved in a wide range of metabolic processes, both in humans and in other organisms, primarily related to the utilization of fats, carbohydrates, and amino acids. ...
*
Disodium phosphate
Disodium phosphate (DSP), or disodium hydrogen phosphate, or sodium phosphate dibasic, is an inorganic compound with the chemical formula . It is one of several sodium phosphates. The salt is known in anhydrous form as well as hydrates , where ...
*
Monopotassium phosphate
Monopotassium phosphate (MKP) (also, potassium dihydrogen phosphate, KDP, or monobasic potassium phosphate) is the inorganic compound with the formula KH2PO4. Together with dipotassium phosphate (K2HPO4.(H2O)x) it is often used as a fertilizer, ...
*
Ferric ammonium citrate
Ammonium ferric citrate (also known as ferric ammonium citrate or ammoniacal ferrous citrate) has the formula . The iron in this compound is trivalent. All three carboxyl groups and the central hydroxyl group of citric acid are deprotonated. A di ...
*
Magnesium sulfate
Magnesium sulfate or magnesium sulphate is a chemical compound, a salt with the formula , consisting of magnesium cations (20.19% by mass) and sulfate anions . It is a white crystalline solid, soluble in water but not in ethanol.
Magnesi ...
*
Calcium chloride
Calcium chloride is an inorganic compound, a Salt (chemistry), salt with the chemical formula . It is a white crystalline solid at room temperature, and it is highly soluble in water. It can be created by neutralising hydrochloric acid with cal ...
*
Zinc sulfate
Zinc sulfate is an inorganic compound with the formula ZnSO4. It forms hydrates ZnSO4·''n''H2O, where ''n'' can range from 0 to 7. All are colorless solids. The most common form includes water of crystallization as the heptahydrate, with the che ...
*
Copper sulfate Copper sulfate may refer to:
* Copper(II) sulfate, CuSO4, a common, greenish blue compound used as a fungicide and herbicide
* Copper(I) sulfate, Cu2SO4, an unstable white solid which is uncommonly used
{{chemistry index
Copper compounds ...
Middlebrook 7H9 broth supports the growth of mycobacterial species when supplemented with nutrients such as glycerol, oleic acid, albumin, and dextrose, except for ''
Mycobacterium bovis
''Mycobacterium bovis'' is a slow-growing (16- to 20-hour generation time) Aerobic organism, aerobic bacterium and the causative agent of tuberculosis in cattle (known as bovine TB). It is related to ''Mycobacterium tuberculosis'', the bacterium ...
'', which is inhibited by glycerol. Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks.
Middlebrook broth is commonly used in the preparation of inocula for antimicrobial assays, biochemical tests (arylsufatase and tellurite reduction), and maintenance of stock strains.
Additionally, 7H9 broth is used as a medium in the
mycobacteria growth indicator tube.
See also
*
Lowenstein-Jensen medium
*
Middlebrook 7H10 agar
Middlebrook 7H10 Agar is a solid growth medium specially used for culture of ''Mycobacterium'', notably ''Mycobacterium tuberculosis''. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly use ...
References
External links
Middlebrook 7H9 Broths
{{DEFAULTSORT:Middlebrook 7h9 Broth
Microbiological media