Middlebrook 7H10 Agar is a solid growth medium specially used for culture of ''Mycobacterium'', notably ''
Mycobacterium tuberculosis
''Mycobacterium tuberculosis'' (M. tb), also known as Koch's bacillus, is a species of pathogenic bacteria in the family Mycobacteriaceae and the causative agent of tuberculosis.
First discovered in 1882 by Robert Koch, ''M. tuberculosis'' ha ...
''. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.
Composition
Ingredients (g/L)
Middlebrook 7H10 Agar Base
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* Ammonium sulfate
Ammonium sulfate (American English and international scientific usage; ammonium sulphate in British English); (NH4)2SO4, is an inorganic salt with a number of commercial uses. The most common use is as a soil fertilizer. It contains 21% nitrogen a ...
, 0.50
* Monopotassium phosphate
Monopotassium phosphate (MKP) (also, potassium dihydrogen phosphate, KDP, or monobasic potassium phosphate) is the inorganic compound with the formula KH2PO4. Together with dipotassium phosphate (K2HPO4.(H2O)x) it is often used as a fertilizer, ...
, 1.50
* Disodium phosphate
Disodium phosphate (DSP), or disodium hydrogen phosphate, or sodium phosphate dibasic, is an inorganic compound with the chemical formula . It is one of several sodium phosphates. The salt is known in anhydrous form as well as hydrates , where ...
, 1.50
* Sodium citrate Sodium citrate may refer to any of the sodium salts of citric acid (though most commonly the third):
* Monosodium citrate
* Disodium citrate
* Trisodium citrate
The three forms of salt are collectively known by the E number E331.
Applications
...
, 0.40
* Magnesium sulfate
Magnesium sulfate or magnesium sulphate is a chemical compound, a salt with the formula , consisting of magnesium cations (20.19% by mass) and sulfate anions . It is a white crystalline solid, soluble in water but not in ethanol.
Magnesi ...
, 0.025
* Zinc sulfate
Zinc sulfate is an inorganic compound with the formula ZnSO4. It forms hydrates ZnSO4·''n''H2O, where ''n'' can range from 0 to 7. All are colorless solids. The most common form includes water of crystallization as the heptahydrate, with the che ...
, 0.001
* Copper sulfate Copper sulfate may refer to:
* Copper(II) sulfate, CuSO4, a common, greenish blue compound used as a fungicide and herbicide
* Copper(I) sulfate, Cu2SO4, an unstable white solid which is uncommonly used
{{chemistry index
Copper compounds ...
, 0.001
* L-Glutamic acid, 0.50
* Ferric ammonium citrate, 0.04
* Pyridoxine hydrochloride, 0.001
* Biotin
Biotin (also known as vitamin B7 or vitamin H) is one of the B vitamins. It is involved in a wide range of metabolic processes, both in humans and in other organisms, primarily related to the utilization of fats, carbohydrates, and amino acids. ...
, 0.0005
* Malachite green
Malachite green is an organic compound that is used as a dyestuff and controversially as an antimicrobial in aquaculture. Malachite green is traditionally used as a dye for materials such as silk, leather, and paper. Despite its name the dye is ...
, 0.00025
* Agar
Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from " ogonori" and " tengusa". As found in nature, agar is a mixture of two components, t ...
, 15.00
* Calcium chloride
Calcium chloride is an inorganic compound, a Salt (chemistry), salt with the chemical formula . It is a white crystalline solid at room temperature, and it is highly soluble in water. It can be created by neutralising hydrochloric acid with cal ...
, 0.0005
Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks.
See also
* Lowenstein-Jensen medium
* Middlebrook 7H9 Broth
References
External links
Middlebrook 7H10 Agar
Traditional Microbiology
{{Growth media
Microbiological media