Structure-Based Assignment (SBA) is a technique to accelerate the resonance assignment which is a key bottleneck of NMR (
Nuclear magnetic resonance
Nuclear magnetic resonance (NMR) is a physical phenomenon in which nuclei in a strong constant magnetic field are disturbed by a weak oscillating magnetic field (in the near field) and respond by producing an electromagnetic signal with a ...
) structural biology. A homologous (similar) protein is used as a template to the target protein in SBA. This template protein provides prior structural information about the target protein and leads to faster resonance assignment . By analogy, in
X-ray Crystallography
X-ray crystallography is the experimental science of determining the atomic and molecular structure of a crystal, in which the crystalline structure causes a beam of incident X-rays to Diffraction, diffract in specific directions. By measuring th ...
, the molecular replacement technique allows solution of the crystallographic phase problem when a homologous structural model is
known, thereby facilitating rapid structure determination. Some of the SBA algorithms
are CAP which is an
RNA
Ribonucleic acid (RNA) is a polymeric molecule that is essential for most biological functions, either by performing the function itself (non-coding RNA) or by forming a template for the production of proteins (messenger RNA). RNA and deoxyrib ...
assignment algorithm which performs an exhaustive search over
all permutations, MARS which is a program for robust automatic backbone assignment and Nuclear Vector Replacement (NVR) which is a molecular replacement like approach for SBA of resonances and
sparse Nuclear Overhauser Effect (NOE)'s.
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References
Nuclear magnetic resonance
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