''Metasolibacillus'' is a genus of Gram-Positive rod-shaped bacteria in the family ''

Caryophanaceae The ''Caryophanaceae'' is a family of Gram-positive bacteria. In 2020, the now defunct family ''Planococcaceae'' was merged into ''Caryophanaceae'' to rectify a nomenclature anomaly. The type genus of this family is ''Caryophanon.'' The family ' ...

'' from the order Caryophanales''.'' The type species of this genus is ''Metasolibacillus meyeri.'' Members of ''Metasolibacillus'' are previously species belonging to '' Lysinibacillus'' and '' Bacillus.'' Instead of branching with species from their respective genus, these four species formed a monophyletic branch in various phylogenetic trees constructed based on conserved genome sequences, indicating their phylogenetic relatedness. The family ''

'' encompassed many branching anomalies such as this one, partially due to the reliance on 16S rRNA sequences as a method for classification, which is known to have low resolution power and give differing results depending on the algorithm used. In 2020, a comparative genomic study emended the family, resulting in the establishment of three new genera, including ''Metasolibacillus''. The name ''Metasolibacillus'' is derived from the Greek adjective ''meta'', translating into "besides" and the Latin term ''

Solibacillus ''Solibacillus'' is a genus of Gram positive, rod shaped, spore-forming bacteria. The first member of ''Solibacillus'' was first isolated in 1999, and was originally called ''Bacillus silvestris''. However, further studies on ''B. silvestris'' f ...

'', referring the genus name. Together, ''Metasolibacillus'' can be translated as a genus besides ''Solibacillus''.

Biochemical Characteristics and Molecular Signatures

Members of this genus are aerobic, motile and endospore-forming. ''Metasolibacillus'' can grow in temperatures ranging from 10°C to 45°C but the optimal growth temperature is in the range of 30-37°C. Studied species are reported to be positive for catalase and

Voges–Proskauer test Voges–Proskauer or VP is a test used to detect acetoin in a bacterial broth culture. The test is performed by adding 1-Naphthol, alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth, which is a Glucose phosphate broth, glucose-ph ...

s. 12 conserved signature indels (CSIs) were identified as exclusively present in this genus in the following proteins: DUF456 domain-containing protein, toxic anion resistance protein, undecaprenyldiphosphomuramoylpentapeptide beta-N-acetylglucosaminyltransferase, c-type cytochrome biogenesis protein CcsB, thiol-disulfide oxidoreductase ResA, hypothetical proteins,

arginase Arginase (, ''arginine amidinase'', ''canavanase'', ''L-arginase'', ''arginine transamidinase'') is a manganese-containing enzyme. The reaction catalyzed by this enzyme is: : arginine + H2O → ornithine + urea It is the final enzyme of the ure ...

, preprotein translocase subunit SecY, ATP-binding cassette domain-containing protein, and purine permease. These CSIs were identified through genomic analysis and serve as a reliable molecular means for identifying and distinguishing members of this genus from other genera within the family ''Caryophanaceae'' and all other bacteria.

Taxonomy

''Metasolibacillus'', as of 2021, contains one species with a validly published name. This genus was identified as a

monophyletic In cladistics for a group of organisms, monophyly is the condition of being a clade—that is, a group of taxa composed only of a common ancestor (or more precisely an ancestral population) and all of its lineal descendants. Monophyletic gro ...

clade and phylogenetically unrelated to other species in the family ''Caryophanaceae'' in studies examining the taxonomic relationships within the family. There are three species that are non-validly published, but are considered to be members of this genus due to shared molecular markers and grouping on phylogenetic trees with the type species.

References

{{reflist Bacillales Bacteria genera