''Metasolibacillus'' is a genus of

gram-positive In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. The Gram stain is ...

rod-shaped bacteria in the family ''

Caryophanaceae The ''Caryophanaceae'' is a family of Gram-positive bacteria. In 2020, the now defunct family ''Planococcaceae'' was merged into ''Caryophanaceae'' to rectify a nomenclature anomaly. The type genus of this family is ''Caryophanon.'' The family ' ...

'' from the order Caryophanales''.'' The type species of this genus is ''Metasolibacillus meyeri.'' Members of ''Metasolibacillus'' are previously species belonging to ''

Lysinibacillus ''Lysinibacillus'' is a genus of bacteria from the family of Bacillaceae. Members of this genus, in contrast to the type species of the genus ''Bacillus'', contains peptidoglycan with lysine, aspartic acid, alanine and glutamic acid. Phylogeny ...

'' and ''

Bacillus ''Bacillus'', from Latin "bacillus", meaning "little staff, wand", is a genus of Gram-positive, rod-shaped bacteria, a member of the phylum ''Bacillota'', with 266 named species. The term is also used to describe the shape (rod) of other so-sh ...

.'' Instead of branching with species from their respective genus, these four species formed a monophyletic branch in various phylogenetic trees constructed based on conserved genome sequences, indicating their phylogenetic relatedness. The family ''

'' encompassed many branching anomalies such as this one, partially due to the reliance on 16S rRNA sequences as a method for classification, which is known to have low resolution power and give differing results depending on the algorithm used. In 2020, a comparative genomic study emended the family, resulting in the establishment of three new genera, including ''Metasolibacillus''. The name ''Metasolibacillus'' is derived from the Greek adjective ''meta'', translating into "besides" and the Latin term ''

Solibacillus ''Solibacillus'' is a genus of Gram positive, rod shaped, spore-forming bacteria. The first member of ''Solibacillus'' was first isolated in 1999, and was originally called ''Bacillus silvestris''. However, further studies on ''B. silvestris'' f ...

'', referring the genus name. Together, ''Metasolibacillus'' can be translated as a genus besides ''Solibacillus''.

Biochemical characteristics and molecular signatures

Source: Members of this genus are

aerobic Aerobic means "requiring air," in which "air" usually means oxygen. Aerobic may also refer to * Aerobic exercise, prolonged exercise of moderate intensity * Aerobics, a form of aerobic exercise * Aerobic respiration, the aerobic process of cellu ...

, motile and endospore-forming. ''Metasolibacillus'' can grow in temperatures ranging from 10 °C to 45 °C but the optimal growth temperature is in the range of 30-37 °C. Studied species are reported to be positive for catalase and

Voges–Proskauer test Voges–Proskauer or VP is a test used to detect acetoin in a bacterial broth culture. The test is performed by adding alpha-naphthol and potassium hydroxide to the Voges-Proskauer broth, which is a glucose-phosphate broth that has been inocula ...

s. 12

conserved signature indels Conserved signature inserts and deletions (CSIs) in protein sequences provide an important category of molecular markers for understanding phylogenetic relationships. CSIs, brought about by rare genetic changes, provide useful phylogenetic markers ...

(CSIs) were identified as exclusively present in this genus in the following proteins: DUF456 domain-containing protein, toxic anion resistance protein, undecaprenyldiphosphomuramoylpentapeptide beta-N-acetylglucosaminyltransferase,

c-type cytochrome Cytochromes ''c'' (cyt ''c'', c-type cytochromes) cytochromes, or heme-containing proteins, that have heme C Covalent bond, covalently attached to the peptide backbone via one or two thioether bonds. These bonds are in most cases part of a specifi ...

biogenesis protein CcsB, thiol-disulfide oxidoreductase ResA, hypothetical proteins,

arginase Arginase (, ''arginine amidinase'', ''canavanase'', ''L-arginase'', ''arginine transamidinase'') is a manganese-containing enzyme. The reaction catalyzed by this enzyme is: : arginine + H2O → ornithine + urea It is the final enzyme of the ur ...

, preprotein translocase subunit SecY, ATP-binding cassette domain-containing protein, and purine permease. These CSIs were identified through genomic analysis and serve as a reliable molecular means for identifying and distinguishing members of this genus from other genera within the family ''Caryophanaceae'' and all other bacteria.

Classification

''Metasolibacillus'', as of 2021, contains one species with a validly published name. This genus was identified as a

monophyletic In biological cladistics for the classification of organisms, monophyly is the condition of a taxonomic grouping being a clade – that is, a grouping of organisms which meets these criteria: # the grouping contains its own most recent co ...

clade and phylogenetically unrelated to other species in the family ''Caryophanaceae'' in studies examining the taxonomic relationships within the family. There are three species that are non-validly published, but are considered to be members of this genus due to shared molecular markers and grouping on phylogenetic trees with the type species.

Phylogeny

The currently accepted taxonomy is based on the

List of Prokaryotic names with Standing in Nomenclature List of Prokaryotic names with Standing in Nomenclature (LPSN) is an online database that maintains information on the naming and taxonomy of prokaryotes, following the taxonomy requirements and rulings of the International Code of Nomenclatu ...

(LPSN) and

National Center for Biotechnology Information The National Center for Biotechnology Information (NCBI) is part of the National Library of Medicine (NLM), a branch of the National Institutes of Health (NIH). It is approved and funded by the government of the United States. The NCBI is lo ...

(NCBI)

References

{{Taxonbar, from=Q107299015 Bacillales Bacteria genera

Biochemical characteristics and molecular signatures

Classification

Phylogeny

See also

References