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Soft X-ray Microscopy
An X-ray microscope uses electromagnetic radiation in the soft X-ray band to produce images of very small objects. Unlike visible light, X-rays do not reflect or refract easily, and they are invisible to the human eye. Therefore, the basic process of an X-ray microscope is to expose film or use a charge-coupled device (CCD) detector to detect X-rays that pass through the specimen. It is a contrast imaging technology using the difference in absorption of soft X-ray in the water window region (wavelength region: 2.34–4.4 nm, photon energy region: 280 – 530 eV) by the carbon atom (main element composing the living cell) and the oxygen atom (main element for water). Development Early X-ray microscopes by Paul Kirkpatrick and Albert Baez used grazing incidence reflective optics to focus the X-rays, which grazed X-rays off parabolic curved mirrors at a very high angle of incidence. An alternative method of focusing X-rays is to use a tiny fresnel zone plate of co ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Electromagnetic Radiation
In physics, electromagnetic radiation (EMR) is a self-propagating wave of the electromagnetic field that carries momentum and radiant energy through space. It encompasses a broad spectrum, classified by frequency or its inverse, wavelength, ranging from radio waves, microwaves, infrared, visible light, ultraviolet, X-rays, and gamma rays. All forms of EMR travel at the speed of light in a vacuum and exhibit wave–particle duality, behaving both as waves and as discrete particles called photons. Electromagnetic radiation is produced by accelerating charged particles such as from the Sun and other celestial bodies or artificially generated for various applications. Its interaction with matter depends on wavelength, influencing its uses in communication, medicine, industry, and scientific research. Radio waves enable broadcasting and wireless communication, infrared is used in thermal imaging, visible light is essential for vision, and higher-energy radiation, such ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
DESY
DESY, short for Deutsches Elektronen-Synchrotron (English: ''German Electron Synchrotron''), is a national research centre for fundamental science located in Hamburg and Zeuthen near Berlin in Germany. It operates particle accelerators used to investigate the structure, dynamics and function of matter, and conducts a broad spectrum of interdisciplinary scientific research in four main areas: particle physics, particle and high energy physics; photon science; astroparticle physics; and the development, construction and operation of particle accelerators. Its name refers to its first project, an electron synchrotron. DESY is publicly financed by the Federal Republic of Germany and the Federal States of Hamburg and Brandenburg and is a member of the Helmholtz Association. Functions and mission DESY's function is to conduct fundamental research for solely civil and peaceful purposes. It specialises in particle accelerator development, construction and operation, particle physics, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Crystal
A crystal or crystalline solid is a solid material whose constituents (such as atoms, molecules, or ions) are arranged in a highly ordered microscopic structure, forming a crystal lattice that extends in all directions. In addition, macroscopic single crystals are usually identifiable by their geometrical shape, consisting of flat faces with specific, characteristic orientations. The scientific study of crystals and crystal formation is known as crystallography. The process of crystal formation via mechanisms of crystal growth is called crystallization or solidification. The word ''crystal'' derives from the Ancient Greek word (), meaning both "ice" and " rock crystal", from (), "icy cold, frost". Examples of large crystals include snowflakes, diamonds, and table salt. Most inorganic solids are not crystals but polycrystals, i.e. many microscopic crystals fused together into a single solid. Polycrystals include most metals, rocks, ceramics, and ice. A third cat ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
X-ray Crystallography
X-ray crystallography is the experimental science of determining the atomic and molecular structure of a crystal, in which the crystalline structure causes a beam of incident X-rays to Diffraction, diffract in specific directions. By measuring the angles and intensities of the X-ray diffraction, a crystallography, crystallographer can produce a three-dimensional picture of the density of electrons within the crystal and the positions of the atoms, as well as their chemical bonds, crystallographic disorder, and other information. X-ray crystallography has been fundamental in the development of many scientific fields. In its first decades of use, this method determined the size of atoms, the lengths and types of chemical bonds, and the atomic-scale differences between various materials, especially minerals and alloys. The method has also revealed the structure and function of many biological molecules, including vitamins, drugs, proteins and nucleic acids such as DNA. X-ray crystall ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Diffraction
Diffraction is the deviation of waves from straight-line propagation without any change in their energy due to an obstacle or through an aperture. The diffracting object or aperture effectively becomes a secondary source of the Wave propagation, propagating wave. Diffraction is the same physical effect as Wave interference, interference, but interference is typically applied to superposition of a few waves and the term diffraction is used when many waves are superposed. Italian scientist Francesco Maria Grimaldi coined the word ''diffraction'' and was the first to record accurate observations of the phenomenon in 1660 in science, 1660. In classical physics, the diffraction phenomenon is described by the Huygens–Fresnel principle that treats each point in a propagating wavefront as a collection of individual spherical wavelets. The characteristic pattern is most pronounced when a wave from a Coherence (physics), coherent source (such as a laser) encounters a slit/aperture tha ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chemical Element
A chemical element is a chemical substance whose atoms all have the same number of protons. The number of protons is called the atomic number of that element. For example, oxygen has an atomic number of 8: each oxygen atom has 8 protons in its atomic nucleus, nucleus. Atoms of the same element can have different numbers of neutrons in their nuclei, known as isotopes of the element. Two or more atoms can combine to form molecules. Some elements form Homonuclear molecule, molecules of atoms of said element only: e.g. atoms of hydrogen (H) form Diatomic molecule, diatomic molecules (H). Chemical compounds are substances made of atoms of different elements; they can have molecular or non-molecular structure. Mixtures are materials containing different chemical substances; that means (in case of molecular substances) that they contain different types of molecules. Atoms of one element can be transformed into atoms of a different element in nuclear reactions, which change an atom's at ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescence
Fluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation. When exposed to ultraviolet radiation, many substances will glow (fluoresce) with colored visible light. The color of the light emitted depends on the chemical composition of the substance. Fluorescent materials generally cease to glow nearly immediately when the radiation source stops. This distinguishes them from the other type of light emission, phosphorescence. Phosphorescent materials continue to emit light for some time after the radiation stops. This difference in duration is a result of quantum spin effects. Fluorescence occurs when a photon from incoming radiation is absorbed by a molecule, exciting it to a higher energy level, followed by the emission of light as the molecule returns to a lower energy state. The emitted light may have a longer wavelength and, therefore, a lower photon energy than the absorbed radi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cryo-electron Microscopy
Cryogenic electron microscopy (cryo-EM) is a transmission electron microscopy technique applied to samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. An aqueous sample solution is applied to a grid-mesh and plunge-frozen in liquid ethane or a mixture of liquid ethane and propane. While development of the technique began in the 1970s, recent advances in detector technology and software algorithms have allowed for the determination of biomolecular structures at near-atomic resolution. This has attracted wide attention to the approach as an alternative to X-ray crystallography or NMR spectroscopy in the structural biology field. In 2017, the Nobel Prize in Chemistry was awarded to Jacques Dubochet, Joachim Frank, and Richard Henderson "for developing cryo-electron microscopy for the high-resolution structure determination of biomolecules in solution." '' Nature Methods'' also named cryo- ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Electron Microscopy
An electron microscope is a microscope that uses a beam of electrons as a source of illumination. It uses electron optics that are analogous to the glass lenses of an optical light microscope to control the electron beam, for instance focusing it to produce magnified images or electron diffraction patterns. As the wavelength of an electron can be up to 100,000 times smaller than that of visible light, electron microscopes have a much higher resolution of about 0.1 nm, which compares to about 200 nm for light microscopes. ''Electron microscope'' may refer to: * Transmission electron microscope (TEM) where swift electrons go through a thin sample * Scanning transmission electron microscope (STEM) which is similar to TEM with a scanned electron probe * Scanning electron microscope (SEM) which is similar to STEM, but with thick samples * Electron microprobe similar to a SEM, but more for chemical analysis * Low-energy electron microscope (LEEM), used to image surfaces * ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Electron Microscope
An electron microscope is a microscope that uses a beam of electrons as a source of illumination. It uses electron optics that are analogous to the glass lenses of an optical light microscope to control the electron beam, for instance focusing it to produce magnified images or electron diffraction patterns. As the wavelength of an electron can be up to 100,000 times smaller than that of visible light, electron microscopes have a much higher Angular resolution, resolution of about 0.1 nm, which compares to about 200 nm for optical microscope, light microscopes. ''Electron microscope'' may refer to: * Transmission electron microscopy, Transmission electron microscope (TEM) where swift electrons go through a thin sample * Scanning transmission electron microscopy, Scanning transmission electron microscope (STEM) which is similar to TEM with a scanned electron probe * Scanning electron microscope (SEM) which is similar to STEM, but with thick samples * Electron microprobe sim ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Brookhaven National Laboratory
Brookhaven National Laboratory (BNL) is a United States Department of Energy national laboratories, United States Department of Energy national laboratory located in Upton, New York, a hamlet of the Brookhaven, New York, Town of Brookhaven. It was formally established in 1947 at the site of Camp Upton, a former List of United States Army installations, U.S. Army base on Long Island. Located approximately 60 miles east of New York City, it is managed by Stony Brook University and Battelle Memorial Institute. Research at BNL includes nuclear and high energy physics, energy science and technology, environmental and bioscience, nanoscience, and national security. The 5,300 acre campus contains several large research facilities, including the Relativistic Heavy Ion Collider and National Synchrotron Light Source II. Seven Nobel Prizes have been awarded for work conducted at Brookhaven Lab. Overview BNL operations are overseen by a Department of Energy Site office, is staffed by approx ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
National Synchrotron Light Source
The National Synchrotron Light Source (NSLS) at Brookhaven National Laboratory (BNL) in Upton, New York was a national user research facility funded by the U.S. Department of Energy (DOE). Built from 1978 through 1984, and officially shut down on September 30, 2014, the NSLS was considered a second-generation synchrotron. The NSLS experimental floor consisted of two electron storage rings: an X-ray ring and a VUV ( vacuum ultraviolet) ring which provided intense, focused light spanning the electromagnetic spectrum from the infrared through X-rays. The properties of this light and the specially designed experimental stations, called beamlines, allowed scientists in many fields of research to perform experiments not otherwise possible at their own laboratories. History Ground was broken for the NSLS on September 28, 1978. The VUV ring began operations in late 1982 and the X-ray ring was commissioned in 1984. In 1986, a second phase of construction expanded the NSLS by , which add ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
