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MIQE
The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting quantitative real-time PCR experiments and data, as devised by Bustin et al. in 2009. They were devised after a paper was published in 2002 that claimed to detect measles virus in children with autism through the use of RT-qPCR, but the results proved to be completely unreproducible by other scientists. The authors themselves also did not try to reproduce them and the raw data was found to have a large amount of errors and basic mistakes in analysis. This incident prompted Stephen Bustin to create the MIQE guidelines to provide a baseline level of quality for qPCR data published in scientific literature. Purpose The MIQE guidelines were created due to the low quality of qPCR data submitted to academic journals at the time, which was only becoming more common as Next Generation Sequencing machinery allowed for such experiments to be ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Stephen Bustin Stephen Andrew Bustin (born 1954) is a British scientist, former professor of molecular sciences at Queen Mary University of London from 2004 to 2012, as well as visiting professor at Middlesex University, beginning in 2006. In 2012 he was appointed Professor of Allied Health and Medicine at Anglia Ruskin University. He is known for his research into polymerase chain reaction, and has written a book on the topic, entitled ''A-Z of Quantitative PCR''. This book has been called "the bible of qPCR." Education Bustin obtained his B.A. and PhD from Trinity College, Dublin in molecular genetics. Career Following the merger with St Bartholomew's Medical College and Queen Mary University of London, Bustin was promoted to Reader in Molecular Medicine in 2002, followed by the award of a personal chair as Professor of Molecular Science in 2004 at Barts and The London School of Medicine and Dentistry. , Bustin held the position of Professor of Molecular Medicine at Anglia Ruskin Univer ... [...More Info...] [...Related Items...] |