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MIQE
The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines are a set of protocols for conducting and reporting Real-time polymerase chain reaction, quantitative real-time PCR experiments and data, as devised by Bustin et al. in 2009. They were devised after a paper was published in 2002 that claimed to detect measles virus in children with autism through the use of RT-qPCR, but the results proved to be completely unreproducible by other scientists. The authors themselves also did not try to reproduce them and the raw data was found to have a large amount of errors and basic mistakes in analysis. This incident prompted Stephen Bustin to create the MIQE guidelines to provide a baseline level of quality for qPCR data published in scientific literature. Purpose The MIQE guidelines were created due to the low quality of qPCR data submitted to academic journals at the time, which was only becoming more common as Next Generation Sequencing machiner ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Stephen Bustin
Stephen Andrew Bustin (born 1954) is a British scientist, former professor of molecular sciences at Queen Mary University of London from 2004 to 2012, as well as visiting professor at Middlesex University, beginning in 2006. In 2012 he was appointed Professor of Allied Health and Medicine at Anglia Ruskin University. He is known for his research into polymerase chain reaction, and has written a book on the topic, entitled ''A-Z of Quantitative PCR''. This book has been called "the bible of qPCR." Education Bustin obtained his B.A. and PhD from Trinity College, Dublin in molecular genetics. Career In 2023, he was elected a member of the Academia Europaea. Research His research group’s general areas of interest are the small and large bowel, as well as colorectal cancer with particular emphasis on investigating the process of invasion and metastasis. An important aim is to translate molecular techniques into clinical practice by including molecular parameters into clinical tumor ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Real-time Polymerase Chain Reaction
A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively and semi-quantitatively (i.e., above/below a certain amount of DNA molecules). Two common methods for the detection of PCR products in real-time PCR are (1) non-specific fluorescent dyes that Intercalation (biochemistry), intercalate with any double-stranded DNA and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescence, fluorescent reporter, which permits detection only after nucleic acid hybridisation, hybridization of the probe with its complementary sequence. The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines propose that the abb ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Photobiomodulation
Low-level laser therapy (LLLT), cold laser therapy or photobiomodulation (PBM) is a medical treatment approach that applies low-level (low- power) lasers or light-emitting diodes (LEDs) to the surface of the body. Whereas high-power lasers are used in laser medicine to cut or destroy tissue, it is claimed that application of low-power lasers stimulates healing, relieves pain, and enhances cell function. Described sometimes as Low-level Red-light Therapy (LLRL), has effects that appear to be limited to a specified set of wavelengths. The effectiveness of red light therapy for treating various conditions is still under investigation. Repeated low level red light therapy may be effective for controlling myopia in children. Several such devices are cleared by the United States Food and Drug Administration (FDA), and low level red light therapy is being tested for treating a range of medical problems including rheumatoid arthritis and oral mucositis. Mechanism Research is on ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
COVID-19
Coronavirus disease 2019 (COVID-19) is a contagious disease caused by the coronavirus SARS-CoV-2. In January 2020, the disease spread worldwide, resulting in the COVID-19 pandemic. The symptoms of COVID‑19 can vary but often include fever, fatigue, cough, breathing difficulties, anosmia, loss of smell, and ageusia, loss of taste. Symptoms may begin one to fourteen days incubation period, after exposure to the virus. At least a third of people who are infected asymptomatic, do not develop noticeable symptoms. Of those who develop symptoms noticeable enough to be classified as patients, most (81%) develop mild to moderate symptoms (up to mild pneumonia), while 14% develop severe symptoms (dyspnea, hypoxia (medical), hypoxia, or more than 50% lung involvement on imaging), and 5% develop critical symptoms (respiratory failure, shock (circulatory), shock, or organ dysfunction, multiorgan dysfunction). Older people have a higher risk of developing severe symptoms. Some complicati ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Assay
An assay is an investigative (analytic) procedure in laboratory medicine, mining, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity. The measured entity is often called the analyte, the measurand, or the target of the assay. The analyte can be a drug, biochemical substance, chemical element or compound, or cell in an organism or organic sample. An assay usually aims to measure an analyte's intensive property and express it in the relevant measurement unit (e.g. molarity, density, functional activity in enzyme international units, degree of effect in comparison to a standard, etc.). If the assay involves exogenous reactants (the reagents), then their quantities are kept fixed (or in excess) so that the quantity and quality of the target are the only limiting factors. The difference in the assay outcome is used to deduce the unknown quality ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gene (journal)
''Gene'' is a peer-reviewed scientific journal in genetics and molecular biology, focusing on the cloning, structure, function, as well as the biomedical and biotechnological importance of genes. The scope of the journal includes all biological organisms including viruses, prokaryotes, and eukaryotes. It is organized into topics, which include Human Genetics, Cancer Genetics, Neurogenetics, Animal Genetics, Genome Editing, Molecular Ecology, Plant Genetics, Parasitology and Virology, as well as Microbiology. The journal is part of the Gene Family of journals and was established in 1976. It is published by Elsevier Elsevier ( ) is a Dutch academic publishing company specializing in scientific, technical, and medical content. Its products include journals such as ''The Lancet'', ''Cell (journal), Cell'', the ScienceDirect collection of electronic journals, ... and the editor-in-chief is Marianna Kruithof-de Julio ( University of Bern) The Gene Family of journals compris ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
BioTechniques
''BioTechniques: the International Journal of Life Science Methods'' is a peer-reviewed open-access scientific journal published by Future Science Group. It covers laboratory methods and techniques that are of broad interest to professional life scientists, as well as scientists from other disciplines (e.g. physics, chemistry, engineering, computer science Computer science is the study of computation, information, and automation. Computer science spans Theoretical computer science, theoretical disciplines (such as algorithms, theory of computation, and information theory) to Applied science, ...s) interested in life science applications of their technologies. The journal was established in 1983 by Eaton Associates, which was acquired in 2001 by Informa. The journal was then acquired by Future Science Group in 2018. It is distributed in both print and online form. The journal is supported by print and website advertising, and as of January 2019, began charging arti ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mobile App
A mobile application or app is a computer program or software application designed to run on a mobile device such as a smartphone, phone, tablet computer, tablet, or smartwatch, watch. Mobile applications often stand in contrast to desktop applications which are designed to run on desktop computers, and web applications which run in mobile web browsers rather than directly on the mobile device. Apps were originally intended for productivity assistance such as email, calendar, and contact databases, but the public demand for apps caused rapid expansion into other areas such as mobile games, factory automation, GPS and location-based services, order-tracking, and ticket purchases, so that there are now millions of apps available. Many apps require Internet access. Apps are generally downloaded from app stores, which are a type of digital distribution platforms. The term "app", short for "Application software, application", has since become very popular; in 2010, it was listed as " ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bio-Rad
Bio-Rad Laboratories, Inc. is an American developer and manufacturer of specialized technological products for the life science research and clinical diagnostics markets. The company was founded in 1952 in Berkeley, California, by husband and wife team David and Alice Schwartz, both graduates of the University of California, Berkeley. Bio-Rad is based in Hercules, California, and has operations worldwide. Business segments Bio-Rad’s life science products primarily include instruments, software, consumables, reagents, and content for the areas of cell biology, gene expression, protein purification, protein quantitation, drug discovery and manufacture, food safety, and science education. These products are based on technologies to separate, purify, identify, analyze, and amplify biological materials such as antibodies, proteins, nucleic acids, cells, and bacteria.Bio-Rad Annual Report 2016 , http://www.bio-rad.com/webroot/web/pdf/corporate/annualreport/Annual_Report_2016. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Genetic Engineering & Biotechnology News
Mary Ann Liebert, Inc. is a publishing company founded by its president, Mary Ann Liebert, in 1980 and a subsidiary of Sage Publishing. The company publishes peer-reviewed academic journals, books, and trade magazines in the areas of biotechnology, biomedical sciences, medical research, and life sciences; clinical medicine, surgery, and nursing; technology and engineering; environmental science; public health and policy; law, regulation, and education. The company's headquarters is in New Rochelle, New York. The company has been described as the first to establish a specialty in genetic engineering. Publications Eschewing traditional market research, the publisher seeks out niche topics overlooked by larger publishers. Its portfolio of more than ninety peer-reviewed journals includes: Publications focused on topics outside of the medical field include ''Westchester Wag'', which covers the social scene in Westchester County, New York, and ''Rinkmagazine'', a skating perio ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
New England Biolabs
New England Biolabs (NEB) is an American life sciences company which produces and supplies recombinant and native enzyme reagents for life science research. It also provides products and services supporting genome editing, synthetic biology and next-generation sequencing. NEB also provides free access to research tools such as REBASE, InBASE, and Polbase. About The company was founded in 1974 by Donald "Don" Comb, a Harvard Medical School professor, as a cooperative laboratory of experienced scientists and initially produced restriction enzymes on a commercial scale. Comb held the CEO title until 2005 when, at 78 years old, he moved from management back into research at the firm. NEB received approximately $1.7 million in Small Business Innovation Research (SBIR) grants between 2009 and 2013 for this research. NEB produces 230 recombinant and 30 native restriction enzymes for genomic research, as well as nicking enzymes and DNA methylases. It pursues research in areas r ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Scientific Reports
''Scientific Reports'' is a peer-reviewed open-access scientific mega journal published by Nature Portfolio, covering all areas of the natural sciences. The journal was established in 2011. The journal states that their aim is to assess solely the scientific validity of a submitted paper, rather than its perceived importance, significance, or impact. In September 2016, the journal became the largest in the world by number of articles, overtaking '' PLOS ONE''. Abstracting and indexing The journal is abstracted and indexed in the Chemical Abstracts Service, the Science Citation Index Expanded, and selectively in Index Medicus/MEDLINE/PubMed. According to the ''Journal Citation Reports'', the journal has a 2023 impact factor 3.8. Reviewing policy The ''Guide to Referees'' states that to be published, "a paper must be scientifically valid and technically sound in methodology and analysis", and reviewers have to ensure manuscripts "are not assessed based on their perceived impor ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
