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Kurzrock–Miller Test
The Kurzrock–Miller test is an in-vitro test of sperm–mucus interaction. It consists of establishing an interface between cervical mucus and liquefied semen. It is one of the tests used for investigating infertility. Etymology The test is named after Raphael Kurzrock and Thomas Grier Miller. Interpretation # Negative test: Spermatozoa congregate on semen side of the interface, but do not penetrate it. # Abnormal: Spermatozoa penetrate the mucus, but rapidly become immobile or acquire shaking movement. # Poor: after penetration, spermatozoa do not progress farther than 500 μm. # Normal: Spermatozoa penetrate the mucus and >90% are motile with definite progression, crossing 3 cm at 30 minutes.Padubidri; Daftary (2011). ''Shaw's Textbook of Gynaecology, 15e.'' p. 204. See also *Hamster egg penetration test *Postcoital test *Sperm–cervical mucus contact test References {{DEFAULTSORT:Kurzrock-Miller test Fertility medicine Medical tests ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
In-vitro
''In vitro'' (meaning ''in glass'', or ''in the glass'') studies are performed with cells or biological molecules outside their normal biological context. Colloquially called " test-tube experiments", these studies in biology and its subdisciplines are traditionally done in labware such as test tubes, flasks, Petri dishes, and microtiter plates. Studies conducted using components of an organism that have been isolated from their usual biological surroundings permit a more detailed or more convenient analysis than can be done with whole organisms; however, results obtained from ''in vitro'' experiments may not fully or accurately predict the effects on a whole organism. In contrast to ''in vitro'' experiments, ''in vivo'' studies are those conducted in living organisms, including humans, known as clinical trials, and whole plants. Definition ''In vitro'' (Latin for "in glass"; often not italicized in English usage) studies are conducted using components of an organism that ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cervical Mucus
The cervix (: cervices) or cervix uteri is a dynamic fibromuscular sexual organ of the female reproductive system that connects the vagina with the uterine cavity. The human female cervix has been documented anatomically since at least the time of Hippocrates, over 2,000 years ago. The cervix is approximately 4 cm long with a diameter of approximately 3 cm and tends to be described as a cylindrical shape, although the front and back walls of the cervix are contiguous. The size of the cervix changes throughout a woman's life cycle. For example, women in the fertile years of their reproductive cycle tend to have larger cervixes than postmenopausal women; likewise, women who have produced offspring have a larger cervix than those who have not. In relation to the vagina, the part of the cervix that opens to the uterus is called the ''internal os'' and the opening of the cervix in the vagina is called the ''external os''. Between them is a conduit commonly called the cerv ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Semen
Semen, also known as seminal fluid, is a bodily fluid that contains spermatozoon, spermatozoa which is secreted by the male gonads (sexual glands) and other sexual organs of male or hermaphrodite, hermaphroditic animals. In humans and placental mammals, seminal fluid is ejaculation, ejaculated through the penis and contains Proteolytic enzyme, proteolytic and other enzymes as well as fructose, which promote the survival of spermatozoa and provide a medium through which they can move or "swim" from the vagina into the uterus to fertilization, fertilize the female ovum and form a zygote. semen collection, Semen is collected from animals for artificial insemination or cryoconservation of genetic material. Cryoconservation of animal genetic resources is a practice that calls for the collection of semen in efforts for conservation of a particular breed. Physiology Fertilization Depending on the species, spermatozoa can fertilize ova externally or internally. In external fertiliz ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Infertility
In biology, infertility is the inability of a male and female organism to Sexual reproduction, reproduce. It is usually not the natural state of a healthy organism that has reached sexual maturity, so children who have not undergone puberty, which is the body's start of fertility, reproductive capacity, are excluded. It is also a normal state in women after menopause. In humans, ''infertility'' is defined as the inability to become pregnant after at least one year of unprotected and regular sexual intercourse involving a male and female partner. There are many causes of infertility, including some that Assisted reproductive technology, medical intervention can treat. Estimates from 1997 suggest that worldwide about five percent of all heterosexual couples have an unresolved problem with infertility. Many more couples, however, experience involuntary childlessness for at least one year, with estimates ranging from 12% to 28%. Male infertility is responsible for 20–30% of infert ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Hamster Egg Penetration Test
The hamster zona-free ovum test (HZFO test), or hamster egg-penetration test, or sometimes just hamster test, is an in-vitro test used to study physiological profile of spermatozoa. The primary application of the test is to diagnose male infertility caused by sperm unable to penetrate the ova. The test has limited value, due to expense and a high false negative rate. Procedure In this test, sperm are incubated with several hamster eggs. After seven to twenty hours, the number of sperm penetrations per egg is measured. The hamster eggs have had the zona pellucida, the outer membrane, removed—hence, ''zona-free''. Having all eggs penetrated by multiple sperm is considered to be a positive sign for fertility. Results suggest that men whose sperm fail the hamster test are a third as fertile than those whose sperm pass. Although medical professionals often present the procedure as unable to create an embryo, these claims are not technically correct. If the human sperm succeeds ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Postcoital Test
The postcoital test (PCT), also known as the Sims test, Hühner test ,Sims–Hühner test, or cervical mucous penetration assay is a test in the evaluation of infertility. The test examines interaction between sperm and mucus of the cervix. The PCT examines sperm survival in cervical mucus during the pre-ovulatory period and determines whether sperm are migrating into the female reproductive system. It does not predict whether pregnancy can occur. The test is performed 1 to 2 days before ovulation, when estrogen-stimulated cervical mucus is abundant. Basal body temperatures or the midcycle luteinizing hormone surge may be used to determine the timing of the PCT. Mucus is withdrawn from the endocervical canal within 8 hours of coitus and examined. The presence of any forwardly motile sperm in alkaline mucus suggests adequate coital technique and a normal cervical mucus–sperm interaction. Procedure The PCT is scheduled close to ovulation when mucus is abundant, and the infertile c ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sperm–cervical Mucus Contact Test
The sperm–cervical mucus contact test is an in-vitro slide test used for detecting antispermal antibodies. It is one of the investigations done for infertility. It should not be confused with Kurzrock–Miller test, where there is interface between the two materials; whereas in this test the materials are thoroughly mixed. Procedure Equal quantities of sperm and mucus are mixed, so there is no interface. If there are antispermal antibodies present, more than 25% sperms show shaky movements.Padubidri; Daftary (2011). ''Shaw's Textbook of Gynaecology, 15e.'' p. 204. Cross-checking the cervical mucus with another donor semen will confirm whether antibodies are cervical or seminal. See also * Hamster egg penetration test *Postcoital test The postcoital test (PCT), also known as the Sims test, Hühner test ,Sims–Hühner test, or cervical mucous penetration assay is a test in the evaluation of infertility. The test examines interaction between sperm and mucus of the cervix. The ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fertility Medicine
Reproductive endocrinology and infertility (REI) is a surgical subspecialty of obstetrics and gynecology that trains physicians in reproductive medicine addressing hormonal functioning as it pertains to reproduction as well as the issue of infertility. While most REI specialists primarily focus on the treatment of infertility, reproductive endocrinologists are trained to also test and treat hormonal dysfunctions in females and males outside infertility. Reproductive endocrinologists have specialty training (residency) in obstetrics and gynecology (ob-gyn) before they undergo sub-specialty training (fellowship) in REI. Reproductive surgery is a related specialty, where a physician in ob-gyn or urology further specializes to operate on anatomical disorders that affect fertility. Certification In a number of countries, the pathway to become a subspecialist in REI is regulated. Thus, in the United States, for instance, the American Board of Obstetrics and Gynecology (ABOG) and Amer ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
