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Joshua R. Sanes
Joshua R Sanes (born 1949) is an American neurobiologist who is known for his contributions to the understanding of synapse development. Throughout his career, Sanes has been the recipient of various awards and honors, including membership to the U.S. National Academy of Sciences. His research involves an interdisciplinary approach which focuses mainly on the formation of synapses at the neuromuscular junction by combining the sciences of psychology, chemistry, biology, and engineering to study these circuits and employ molecular and genetic imaging to understand their function. Sanes currently lives in Boston, Massachusetts with his wife, Susan, and their two children. Early life and education Sanes was born in Buffalo, New York in 1949. Sanes' father owned an automobile parts store, and his mother attended school to become a speech pathologist. His father and mother both loved to read and so they kept plenty of books around the house. This is what sparked Sanes interest in the b ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Buffalo, New York
Buffalo is a Administrative divisions of New York (state), city in the U.S. state of New York (state), New York and county seat of Erie County, New York, Erie County. It lies in Western New York at the eastern end of Lake Erie, at the head of the Niagara River on the Canada–United States border, Canadian border. With a population of 278,349 according to the 2020 census, Buffalo is the List of municipalities in New York, second-most populous city in New York State after New York City, and the List of United States cities by population, 82nd-most populous city in the U.S. Buffalo is the primary city of the Buffalo–Niagara Falls metropolitan area, which had an estimated population of 1.1 million in 2020, making it the List of metropolitan statistical areas, 49th-largest metro area in the U.S. Before the 17th century, the region was inhabited by nomadic Paleo-Indians who were succeeded by the Neutral Confederacy, Neutral, Erie people, Erie, and Iroquois nations. In the early 1 ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Harvard
Harvard University is a private Ivy League research university in Cambridge, Massachusetts, United States. Founded in 1636 and named for its first benefactor, the Puritan clergyman John Harvard, it is the oldest institution of higher learning in the United States. Its influence, wealth, and rankings have made it one of the most prestigious universities in the world. Harvard was founded and authorized by the Massachusetts General Court, the governing legislature of colonial-era Massachusetts Bay Colony. While never formally affiliated with any denomination, Harvard trained Congregational clergy until its curriculum and student body were gradually secularized in the 18th century. By the 19th century, Harvard emerged as the most prominent academic and cultural institution among the Boston elite. Following the American Civil War, under Harvard president Charles William Eliot's long tenure from 1869 to 1909, Harvard developed multiple professional schools, which transfo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Embryological
Embryology (from Greek ἔμβρυον, ''embryon'', "the unborn, embryo"; and -λογία, ''-logia'') is the branch of animal biology that studies the prenatal development of gametes (sex cells), fertilization, and development of embryos and fetuses. Additionally, embryology encompasses the study of congenital disorders that occur before birth, known as teratology. Early embryology was proposed by Marcello Malpighi, and known as preformationism, the theory that organisms develop from pre-existing miniature versions of themselves. Aristotle proposed the theory that is now accepted, epigenesis. Epigenesis is the idea that organisms develop from seed or egg in a sequence of steps. Modern embryology developed from the work of Karl Ernst von Baer, though accurate observations had been made in Italy by anatomists such as Aldrovandi and Leonardo da Vinci in the Renaissance. Comparative embryology Preformationism and epigenesis As recently as the 18th century, the prevail ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Brainbow
Brainbow is a process by which individual neurons in the brain can be distinguished from neighboring neurons using fluorescent proteins. By randomly expressing different ratios of red, green, and blue derivatives of green fluorescent protein in individual neurons, it is possible to flag each neuron with a distinctive color. This process has been a major contribution to the field of neural connectomics. The technique was originally developed in 2007 by a team led by Jeff W. Lichtman and Joshua R. Sanes, both at Harvard University. The original technique has been adapted for use with other model research organisms including the fruit fly (''Drosophila melanogaster''), zebrafish (''Danio rerio''), and ''Arabidopsis thaliana''. While earlier labeling techniques allowed for the mapping of only a few neurons, this new method allows more than 100 differently mapped neurons to be simultaneously and differentially illuminated in this manner. This leads to its characteristic multicolored ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Extracellular Matrix
In biology, the extracellular matrix (ECM), also called intercellular matrix (ICM), is a network consisting of extracellular macromolecules and minerals, such as collagen, enzymes, glycoproteins and hydroxyapatite that provide structural and biochemical support to surrounding cells. Because multicellularity evolved independently in different multicellular lineages, the composition of ECM varies between multicellular structures; however, cell adhesion, cell-to-cell communication and differentiation are common functions of the ECM. The animal extracellular matrix includes the interstitial matrix and the basement membrane. Interstitial matrix is present between various animal cells (i.e., in the intercellular spaces). Gels of polysaccharides and fibrous proteins fill the interstitial space and act as a compression buffer against the stress placed on the ECM. Basement membranes are sheet-like depositions of ECM on which various epithelial cells rest. Each type of connective tissue ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Knockout Mice
A knockout mouse, or knock-out mouse, is a genetically modified mouse (''Mus musculus'') in which researchers have inactivated, or " knocked out", an existing gene by replacing it or disrupting it with an artificial piece of DNA. They are important animal models for studying the role of genes which have been sequenced but whose functions have not been determined. By causing a specific gene to be inactive in the mouse, and observing any differences from normal behaviour or physiology, researchers can infer its probable function. Mice are currently the laboratory animal species most closely related to humans for which the knockout technique can easily be applied. They are widely used in knockout experiments, especially those investigating genetic questions that relate to human physiology. Gene knockout in rats is much harder and has only been possible since 2003. The first recorded knockout mouse was created by Mario R. Capecchi, Martin Evans, and Oliver Smithies in 1989, fo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Agrin
Agrin is a large proteoglycan whose best-characterised role is in the development of the neuromuscular junction during embryogenesis. Agrin is named based on its involvement in the aggregation of acetylcholine receptors during synaptogenesis. In humans, this protein is encoded by the ''AGRN'' gene. This protein has nine domains homologous to protease inhibitors. It may also have functions in other tissues and during other stages of development. It is a major proteoglycan component in the glomerular basement membrane and may play a role in the renal filtration and cell-matrix interactions. Agrin functions by activating the MuSK protein (for Muscle-Specific Kinase), which is a receptor tyrosine kinase required for the formation and maintenance of the neuromuscular junction. Agrin is required to activate MuSK. Agrin is also required for neuromuscular junction formation. Discovery Agrin was first identified by the U.J. McMahan laboratory, Stanford University. Mechani ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Neuromuscular Junction
A neuromuscular junction (or myoneural junction) is a chemical synapse between a motor neuron and a muscle fiber. It allows the motor neuron to transmit a signal to the muscle fiber, causing muscle contraction. Muscles require innervation to function—and even just to maintain muscle tone, avoiding atrophy. In the neuromuscular system, nerves from the central nervous system and the peripheral nervous system are linked and work together with muscles. Synaptic transmission at the neuromuscular junction begins when an action potential reaches the presynaptic terminal of a motor neuron, which activates voltage-gated calcium channels to allow calcium ions to enter the neuron. Calcium ions bind to sensor proteins (synaptotagmins) on synaptic vesicles, triggering vesicle fusion with the cell membrane and subsequent neurotransmitter release from the motor neuron into the synaptic cleft. In vertebrates, motor neurons release acetylcholine (ACh), a small molecule neurotransmitter, which ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Brainbow (Lichtman 2008)
Brainbow is a process by which individual neurons in the brain can be distinguished from neighboring neurons using fluorescent proteins. By randomly expressing different ratios of red, green, and blue derivatives of green fluorescent protein in individual neurons, it is possible to flag each neuron with a distinctive color. This process has been a major contribution to the field of neural connectomics. The technique was originally developed in 2007 by a team led by Jeff W. Lichtman and Joshua R. Sanes, both at Harvard University. The original technique has been adapted for use with other model research organisms including the fruit fly (''Drosophila melanogaster''), zebrafish (''Danio rerio''), and ''Arabidopsis thaliana''. While earlier labeling techniques allowed for the mapping of only a few neurons, this new method allows more than 100 differently mapped neurons to be simultaneously and differentially illuminated in this manner. This leads to its characteristic multicolored a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cellular Biology
Cell biology (also cellular biology or cytology) is a branch of biology that studies the Anatomy, structure, Physiology, function, and behavior of cell (biology), cells. All living organisms are made of cells. A cell is the basic unit of life that is responsible for the living and functioning of organisms. Cell biology is the study of the structural and functional units of cells. Cell biology encompasses both Prokaryote, prokaryotic and Eukaryote, eukaryotic cells and has many subtopics which may include the study of Metabolism, cell metabolism, Signaling pathway, cell communication, cell cycle, biochemistry, and Cell (biology), cell composition. The study of cells is performed using several microscopy techniques, cell culture, and cell fractionation. These have allowed for and are currently being used for discoveries and research pertaining to how cells function, ultimately giving insight into understanding larger organisms. Knowing the components of cells and how cells work is f ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Physiology
Physiology (; ) is the science, scientific study of function (biology), functions and mechanism (biology), mechanisms in a life, living system. As a branches of science, subdiscipline of biology, physiology focuses on how organisms, organ systems, individual organ (biology), organs, cell (biology), cells, and biomolecules carry out chemistry, chemical and physics, physical functions in a living system. According to the classes of organisms, the field can be divided into clinical physiology, medical physiology, Zoology#Physiology, animal physiology, plant physiology, cell physiology, and comparative physiology. Central to physiological functioning are biophysics, biophysical and biochemical processes, homeostasis, homeostatic control mechanisms, and cell signaling, communication between cells. ''Physiological state'' is the condition of normal function. In contrast, ''pathology, pathological state'' refers to abnormality (behavior), abnormal conditions, including human diseases. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Washington University School Of Medicine
Washington University School of Medicine (WashU Medicine) is the medical school of Washington University in St. Louis, located in the Central West End neighborhood of St. Louis, Missouri. Founded in 1891, the School of Medicine shares a campus with Barnes-Jewish Hospital, St. Louis Children's Hospital, and the Alvin J. Siteman Cancer Center. The clinical service is provided by Washington University Physicians, a comprehensive medical and surgical practice providing treatment in more than 75 medical specialties. Washington University Physicians are the medical staff of the school's two teaching hospitals – Barnes-Jewish Hospital and St. Louis Children's Hospital. They also provide inpatient and outpatient care at the St. Louis Veteran's Administration Hospital, hospitals of the BJC HealthCare system, and 35 other office locations throughout the greater St. Louis region. History Medical classes were first held at Washington University in 1891 after the St. Louis Medi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
