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Fuchsin
Fuchsine (sometimes spelled fuchsin) or rosaniline hydrochloride is a magenta dye with chemical formula C20H19N3·HCl."Basic chemical data" ''Discovery Series'' online database, Developmental Therapeutics Program, U.S. National Institutes of Health. Retrieved on 2007-10-08. There are other similar chemical formulations of products sold as fuchsine, and several dozen other synonyms of this molecule. It becomes magenta when dissolved in ; as a , it forms dark |
Staining (biology)
Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues), in cytology (microscopic study of cells), and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level. Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells), or organelles within individual cells. In biochemistry, it involves adding a class-specific ( DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. Light microscopes are used for view ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Schiff Reagent
: The Schiff test is an early organic chemistry named reaction developed by Hugo Schiff, and is a relatively general chemical test for detection of many organic aldehydes that has also found use in the staining of biological tissues. The Schiff reagent is the reaction product of a dye formulation such as fuchsin and sodium bisulfite; pararosaniline (which lacks an aromatic methyl group) and new fuchsin (which is uniformly mono-methylated ''ortho'' to the dye's amine functionalities) are not dye alternatives with comparable detection chemistry. In its use as a qualitative test for aldehydes, the unknown sample is added to the decolorized Schiff reagent; when aldehyde is present a characteristic magenta color develops. Schiff-type reagents are used for various biological tissue staining methods, e.g. Feulgen stain and periodic acid-Schiff stain. Human skin also contains aldehyde functional groups in the termini of saccharides and so is stained as well. Mechanism Fuchsin s ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Carbol Fuchsin
Carbol fuchsin, carbol-fuchsin, carbolfuchsin, or Castellani's paint (CAS ) is a mixture of phenol and basic fuchsin that is used in bacterial staining procedures. It is commonly used in the staining of mycobacteria because it has an affinity for the mycolic acids found in their cell membranes. It is a component of Ziehl–Neelsen stain, a differential stain. Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells' wall lipids than in the acid alcohol. If the bacteria is acid-fast the bacteria will retain the initial red color of the dye because they are able to resist the destaining by acid alcohol (0.4–1% HCl in 70% EtOH). Additionally, it can be used for the staining of bacterial spores. Carbol-fuchsin is also used as a topical antiseptic and antifungal An antifungal medication, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Acid Fuchsine
Acid fuchsin or fuchsine acid, (also called Acid Violet 19 and C.I. 42685) is an acidic magenta dye with the chemical formula C20H17N3Na2O9S3. It is a sodium sulfonate derivative of fuchsine. Acid fuchsin has wide use in histology, and is one of the dyes used in Masson's trichrome stain. This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue with Light Green SF yellowish or methyl blue. It can also be used to identify growing bacteria. See also * New fuchsine New fuchsine is an organic compound with the formula H2N(CH3)C6H3)3Cl. It is a green-colored solid that is used as a dye of the triarylmethane class. It is one of the four components of basic fuchsine, and one of the two that are available as ... * Pararosanilin * Verhoeff’s Stain * Pollen grain staining (Alexander's stai ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ziehl–Neelsen Stain
Ziehl–Neelsen staining is a type of acid-fast stain, first introduced by Paul Ehrlich. Ziehl–Neelsen staining is a bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria. It is named for two German doctors who modified the stain: the bacteriologist Franz Ziehl (1859–1926) and the pathologist Friedrich Neelsen (1854–1898). Mycobacteria In anatomic pathology specimens, immunohistochemistry and modifications of Ziehl–Neelsen staining (such as the Fite-Faraco method) have comparable diagnostic utility. Both of them are superior to traditional Ziehl–Neelsen staining. The genus ''Mycobacterium'' is a slow growing bacteria, made up of small rods that are slightly curved or straight, and are considered to be gram positive. Some mycobacteria are free-living saprophytes, but many are pathogens that cause disease in animals and humans. '' Mycobacterium bovis'' causes tuberculosis in cattle. Since tuberculosis can be spread to humans, milk is pas ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
New Fuchsine
New fuchsine is an organic compound with the formula H2N(CH3)C6H3)3Cl. It is a green-colored solid that is used as a dye of the triarylmethane class. It is one of the four components of basic fuchsine, and one of the two that are available as single dyes. The other is pararosaniline. It is prepared by condensation of ortho-toluidine with formaldehyde. This process initially gives the benzhydrol 4,4'-bis(dimethylamino)benzhydrol, which is further condensed to give the leuco (colorless) tertiary alcohol [(H2N(CH3)C6H3)3COH, which is oxidized in acid to give the dye. Use as dye and stain New fuchsine is used to dye polyacrylonitrile, paper, and leather. In biology, it can be used for staining (biology), staining acid-fast organisms, e.g. by Ziehl–Neelsen stain, and for making Schiff's reagent. As a primary amine, the dye can be diazotized in the laboratory, and the resulting diazonium salt used as a trapping agent in enzyme histochemistry.Lojda Z, Gossrau R, Schiebler TH (19 ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pararosaniline
Pararosaniline, Basic Red 9, or C.I. 42500 is an organic compound with the formula H2NC6H4)3Cl. It is a magenta solid with a variety of uses as a dye. It is one of the four components of basic fuchsine. (The others are rosaniline, new fuchsine and magenta II.) It is structurally related to other triarylmethane dyes called methyl violets including crystal violet, which feature methyl groups on nitrogen. It is prepared by the condensation of aniline and para-aminobenzaldehyde. Alternatively, it arises from the oxidation of 4,4'-bis(aminophenyl)methane in the presence of aniline. Uses *It is used to dye polyacrylonitrile fibers. *Pararosaniline is used as a colorimetric test for aldehydes, in the Schiff test. It is the only basic fuchsine component suitable for making the aldehyde-fuchsine stain for pancreatic islet beta cells. *It has use as an Antischistosomal. Related compounds * 4,4'-Thiodianiline * 4,4'-Methylenedianiline * 4,4'-Oxydianiline * Dapsone Dapsone, als ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Leonhart Fuchs
Leonhart Fuchs (; 17 January 1501 – 10 May 1566), sometimes spelled Leonhard Fuchs and cited in Latin as ''Leonhartus Fuchsius'', was a German physician and botanist. His chief notability is as the author of a large book about plants and their uses as medicines, a herbal, which was first published in 1542 in Latin. It has about 500 accurate and detailed drawings of plants, which were printed from woodcuts. The drawings are the book's most notable advance on its predecessors. Although drawings had been used in other herbal books, Fuchs' book proved and emphasized high-quality drawings as the most telling way to specify what a plant name stands for. Life Fuchs was born in 1501 in Wemding (Marktplatz 5), near Donauwörth in Donau-Ries in the then Duchy of Bavaria, as the youngest son of Johann (Hans) Fuchs and his wife Anna Denten. His father was the town Burgomaster, and both parents came from families of municipal councillors (''Ratsherr''). The exact date of his b ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Aniline
Aniline is an organic compound with the formula C6 H5 NH2. Consisting of a phenyl group attached to an amino group, aniline is the simplest aromatic amine. It is an industrially significant commodity chemical, as well as a versatile starting material for fine chemical synthesis. Its main use is in the manufacture of precursors to polyurethane, dyes, and other industrial chemicals. Like most volatile amines, it has the odor of rotten fish. It ignites readily, burning with a smoky flame characteristic of aromatic compounds. It is toxic to humans. Relative to benzene, it is electron-rich. It thus participates more rapidly in electrophilic aromatic substitution reactions. Likewise, it is also prone to oxidation: while freshly purified aniline is an almost colorless oil, exposure to air results in gradual darkening to yellow or red, due to the formation of strongly colored, oxidized impurities. Aniline can be diazotized to give a diazonium salt, which can then undergo vario ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Magenta II
Magenta () is a color that is variously defined as pinkish- purplish- red, reddish-purplish-pink or mauvish-crimson. On color wheels of the RGB (additive) and CMY (subtractive) color models, it is located exactly midway between red and blue. It is one of the four colors of ink used in color printing by an inkjet printer, along with yellow, black, and cyan, to make all other colors. The tone of magenta used in printing is called "printer's magenta". It is also a shade of purple. Magenta took its name from an aniline dye made and patented in 1859 by the French chemist François-Emmanuel Verguin, who originally called it ''fuchsine''. It was renamed to celebrate the Italian-French victory at the Battle of Magenta fought between the French and Austrians on 4 June 1859 near the Italian town of Magenta in Lombardy. A virtually identical color, called roseine, was created in 1860 by two British chemists, Chambers Nicolson and George Maule. The web color magenta is also ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Magenta
Magenta () is a color that is variously defined as pinkish- purplish- red, reddish-purplish-pink or mauvish- crimson. On color wheels of the RGB (additive) and CMY (subtractive) color models, it is located exactly midway between red and blue. It is one of the four colors of ink used in color printing by an inkjet printer, along with yellow, black, and cyan, to make all other colors. The tone of magenta used in printing is called "printer's magenta". It is also a shade of purple. Magenta took its name from an aniline dye made and patented in 1859 by the French chemist François-Emmanuel Verguin, who originally called it ''fuchsine''. It was renamed to celebrate the Italian-French victory at the Battle of Magenta fought between the French and Austrians on 4 June 1859 near the Italian town of Magenta in Lombardy. A virtually identical color, called roseine, was created in 1860 by two British chemists, Chambers Nicolson and George Maule. The web color magenta is also ca ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Jakub Natanson
Jakub Natanson (20 August 1832 – 14 September 1884) was a Polish chemist and banker, one of the discoverers of Fuchsine. He wrote the first textbook on organic chemistry in the Polish language. Life He was born 20 August 1832 in Warsaw as the son of a banker. From 1852 to 1856 he studied chemistry at the Universität Dorpat (today Tartu, central Estonia) with a master’s degree in 1856, where he synthesized fuchsine in the master’s thesis (published in Liebigs Annalen). He then trained from 1858 to 1862 in Germany, France and Great Britain with leading chemists and in 1862 became Professor of Chemistry at the ''Szkoła Główna Warszawska'' in Warsaw. In 1856 he found two new urea syntheses. He gave up his professorship in 1866 to join the family bank ( Bank Handlowy, today after the merger citi-Handlowy.) He was in the management of various companies (with interests in coal mining, paper, sugar, railroad) and founded, among others, the industrial and agricultural ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
