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Calysta
Calysta is a multinational biotechnology firm based in San Mateo, California. The company develops industrial processes that utilize microorganisms to convert methane into protein for seafood, livestock feed and other food ingredients. It operates a demonstration plant in Teesside, England, that uses methanotroph bacteria to convert methane into single cell protein currently approved for use in fish and livestock feed in the European Union. The firm is a spinout of DNA 2.0, the largest US-based provider of synthetic genes for industrial and academic use. History Calysta was founded in 2012 in Menlo Park, California by Josh Silverman, and is led by CEO Alan Shaw. By June 2013, the firm began working with NatureWorks to use methane fermentation to produce lactic acid. However, its main technology is based on a similar method developed in the 1980s by Statoil, an unrelated and state-owned energy company in Norway. In 2014, Calysta purchased and further developed the technolo ...
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Methylococcus Capsulatus
''Methylococcus capsulatus'' is an obligately methanotrophic gram-negative, non-motile coccoid bacterium. ''M. capsulatus'' are thermotolerant; their cells are encapsulated and tend to have a diplococcoid shape. In addition to methane, ''M. capsulatus'' is able to oxidize some organic hydrogen containing compounds such as methanol. It has been used commercially to produce animal feed from natural gas. Metabolism and genetics ''Methylococcus capsulatus'' is a Type I methanotroph, meaning that it is a member of the Gammaproteobacteria and that it utilizes the ribulose monophosphate pathway (RuMP) for formaldehyde assimilation. Methane is first oxidized to methanol, which then gets converted into formaldehyde. Formaldehyde can then be (1) further oxidized to formate and carbon dioxide for energy production or (2) assimilated into biomass. Various strains of ''M. capsulatus'' have been isolated and studied, particularly ''M. capsulatus'' (Bath), ...
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Single Cell Protein
Single-cell proteins (SCP) or microbial proteins refer to edible unicellular microorganisms. The biomass or protein extract from pure or mixed cultures of algae, yeasts, fungi or bacteria may be used as an ingredient or a substitute for protein-rich foods, and is suitable for human consumption or as animal feeds. Industrial agriculture is marked by a high water footprint, high land use, biodiversity destruction, general environmental degradation and contributes to climate change by emission of a third of all greenhouse gases; production of SCP does not necessarily exhibit any of these serious drawbacks. As of today, SCP is commonly grown on agricultural waste products, and as such inherits the ecological footprint and water footprint of industrial agriculture. However, SCP may also be produced entirely independent of agricultural waste products through autotrophic growth. Thanks to the high diversity of microbial metabolism, autotrophic SCP provides several different modes of growth, ...
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Fish Feed
Manufactured feeds are an important part of modern commercial aquaculture, providing the balanced nutrition needed by farmed fish. The feeds, in the form of granules or pellets, provide the nutrition in a stable and concentrated form, enabling the fish to feed efficiently and grow to their full potential. Many of the fish farmed more intensively around the world today are carnivorous, for example Atlantic salmon, trout, sea bass, and turbot. In the development of modern aquaculture, starting in the 1970s, fishmeal and fish oil were key components of the feeds for these species. They are combined with other ingredients such as vegetable proteins, cereal grains, vitamins and minerals and formed into feed pellets. Wheat, for example, is widely used as it helps to bind the ingredients in the pellets. Other forms of fish feed being used include feeds made entirely with vegetable materials for species such as carp, moist feeds preferred by some species (easier to make but more dif ...
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Privately Held Company
A privately held company (or simply a private company) is a company whose shares and related rights or obligations are not offered for public subscription or publicly negotiated in the respective listed markets, but rather the company's stock is offered, owned, traded, exchanged privately, or Over-the-counter (finance), over-the-counter. In the case of a closed corporation, there are a relatively small number of shareholders or company members. Related terms are closely-held corporation, unquoted company, and unlisted company. Though less visible than their public company, publicly traded counterparts, private companies have major importance in the world's economy. In 2008, the 441 list of largest private non-governmental companies by revenue, largest private companies in the United States accounted for ($1.8 trillion) in revenues and employed 6.2 million people, according to ''Forbes''. In 2005, using a substantially smaller pool size (22.7%) for comparison, the 339 companies on ...
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Statoil
Equinor ASA (formerly Statoil and StatoilHydro) is a Norwegian state-owned multinational energy company headquartered in Stavanger. It is primarily a petroleum company, operating in 36 countries with additional investments in renewable energy. In the 2020 Forbes Global 2000, Equinor was ranked as the 169th-largest public company in the world. the company has 21,126 employees. The current company was formed by the 2007 merger of Statoil with the oil and gas division of Norsk Hydro. As of 2017, the Government of Norway is the largest shareholder with 67% of the shares, while the rest is public stock. The ownership interest is managed by the Norwegian Ministry of Petroleum and Energy. The company is headquartered and led from Stavanger, while most of their international operations are currently led from Fornebu, outside Oslo. The name ''Equinor'' was adopted in 2018 and is formed by combining ''equi'', the root for words such as ''equity'', ''equality'', and ''equilibrium'', a ...
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Fish Farming
upright=1.3, mariculture.html" ;"title="Salmon farming in the sea (mariculture">Salmon farming in the sea (mariculture) at Loch Ainort, Isle of Skye, Scotland Fish farming or pisciculture involves commercial animal husbandry, breeding of fish, usually for food, in fish tanks or artificial pen (enclosure), enclosures such as fish ponds. It is a particular type of aquaculture, which is the controlled cultivation and harvesting of aquatic animals such as fish, crustaceans, molluscs and so on, in natural or pseudo-natural environment. A facility that releases juvenile fish into the wild for recreational fishing or to supplement a species' natural numbers is generally referred to as a fish hatchery. Worldwide, the most important fish species produced in fish farming are carp, catfish, salmon and tilapia. Global demand is increasing for dietary fish protein, which has resulted in widespread overfishing in wild fisheries, resulting in significant decrease in fish stocks and ev ...
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Genetically Modified Food
Genetically modified foods (GM foods), also known as genetically engineered foods (GE foods), or bioengineered foods are foods produced from organisms that have had changes introduced into their DNA using the methods of genetic engineering. Genetic engineering techniques allow for the introduction of new traits as well as greater control over traits when compared to previous methods, such as selective breeding and mutation breeding. The discovery of DNA and the improvement of genetic technology in the 20th century played a crucial role in the development of transgenic technology. In 1988, genetically modified microbial enzymes were first approved for use in food manufacture. Recombinant rennet was used in few countries in the 1990s. Commercial sale of genetically modified foods began in 1994, when Calgene first marketed its unsuccessful Flavr Savr delayed-ripening tomato.Weasel, Lisa H. 2009. ''Food Fray''. Amacom Publishing Most food modifications have primarily focused on ca ...
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Methane Monooxygenase
Methane monooxygenase (MMO) is an enzyme capable of oxidizing the C-H bond in methane as well as other alkanes. Methane monooxygenase belongs to the class of oxidoreductase enzymes (). There are two forms of MMO: the well-studied soluble form (sMMO) and the particulate form (pMMO). The active site in sMMO contains a di-iron center bridged by an oxygen atom (Fe-O-Fe), whereas the active site in pMMO utilizes copper. Structures of both proteins have been determined by X-ray crystallography; however, the location and mechanism of the active site in pMMO is still poorly understood and is an area of active research. The particulate methane monooxygenase and related ammonia monooxygenase are integral membrane proteins, occurring in methanotrophs and ammonia oxidisers, respectively, which are thought to be related. These enzymes have a relatively wide substrate specificity and can catalyse the oxidation of a range of substrates including ammonia, methane, halogenated hydrocarbons, and ...
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Enzyme
Enzymes () are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrate (chemistry), substrates, and the enzyme converts the substrates into different molecules known as product (chemistry), products. Almost all metabolism, metabolic processes in the cell (biology), cell need enzyme catalysis in order to occur at rates fast enough to sustain life. Metabolic pathways depend upon enzymes to catalyze individual steps. The study of enzymes is called ''enzymology'' and the field of pseudoenzyme, pseudoenzyme analysis recognizes that during evolution, some enzymes have lost the ability to carry out biological catalysis, which is often reflected in their amino acid sequences and unusual 'pseudocatalytic' properties. Enzymes are known to catalyze more than 5,000 biochemical reaction types. Other biocatalysts are Ribozyme, catalytic RNA molecules, called ribozymes. Enzymes' Chemical specificity, specific ...
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Methanotroph
Methanotrophs (sometimes called methanophiles) are prokaryotes that metabolize methane as their source of carbon and chemical energy. They are bacteria or archaea, can grow aerobically or anaerobically, and require single-carbon compounds to survive. Methanotrophs are especially common in or near environments where methane is produced, although some methanotrophs can oxidize atmospheric methane. Their habitats include wetlands, soils, marshes, rice paddies, landfills, aquatic systems (lakes, oceans, streams) and more. They are of special interest to researchers studying global warming, as they play a significant role in the global methane budget, by reducing the amount of methane emitted to the atmosphere. Methanotrophy is a special case of methylotrophy, using single-carbon compounds that are more reduced than carbon dioxide. Some methylotrophs, however, can also make use of multi-carbon compounds; this differentiates them from methanotrophs, which are usually fastidious me ...
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Chongqing
Chongqing ( or ; ; Sichuanese pronunciation: , Standard Mandarin pronunciation: ), alternately romanized as Chungking (), is a municipality in Southwest China. The official abbreviation of the city, "" (), was approved by the State Council on 18 April 1997. This abbreviation is derived from the old name of a part of the Jialing River that runs through Chongqing and feeds into the Yangtze River. Administratively, it is one of the four municipalities under the direct administration of the central government of the People's Republic of China (the other three are Beijing, Shanghai, and Tianjin), and the only such municipality located deep inland. The municipality of Chongqing, roughly the size of Austria, includes the city of Chongqing as well as various discontiguous cities. Due to a classification technicality, Chongqing municipality can claim to be the largest city proper in the worldthough it does not have the world's largest urban area. Chongqing is the only city ...
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Temasek Holdings
Temasek Holdings (Private) Limited, or simply Temasek, is a Singaporean state holding company owned by the Government of Singapore. Incorporated on 25 June 1974, Temasek owns and manages a total of US$496.59 billion (S$671 billion) in assets under management (AUM) as of December 2022. Its net portfolio is US$287 billion (S$403 billion) as of 2022, with S$37 billion divested and S$61 billion invested during the year. Its one-year total shareholder return (TSR) was 5.8%, with longer term 10 and 20-year TSRs at 7% and 8% respectively, compounded annually. Its TSR since inception was 14%, compounded over 48 years. Headquartered in Singapore, it has 12 offices in 8 countries around the world, including in Beijing, Hanoi, Mumbai, Shanghai, Shenzhen; and London, Brussels, New York, San Francisco, Washington, D.C. and Mexico City outside Asia. It is an active shareholder and investor, with four key structural trends guiding its long term portfolio construction – Digitisation, Sus ...
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