Agrobacterium Transformation
''Agrobacterium tumefaciens'' (also known as ''Rhizobium radiobacter'') is the causal agent of crown gall disease (the formation of tumours) in over 140 species of eudicots. It is a rod-shaped, Gram-negative soil bacterium. Symptoms are caused by the insertion of a small segment of DNA (known as T-DNA, for 'transfer DNA', not to be confused with tRNA that transfers amino acids during protein synthesis), from a plasmid into the plant cell, which is incorporated at a semi-random location into the plant genome. Plant genomes can be engineered by use of ''Agrobacterium'' for the delivery of sequences hosted in T-DNA binary vectors. ''Agrobacterium tumefaciens'' is an Alphaproteobacterium of the family Rhizobiaceae, which includes the nitrogen-fixing legume symbionts. Unlike the nitrogen-fixing symbionts, tumor-producing ''Agrobacterium'' species are pathogenic and do not benefit the plant. The wide variety of plants affected by ''Agrobacterium'' makes it of great concern to the agr ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Agrobacterium Radiobacter
''Agrobacterium radiobacter'' is the type species of the genus ''Agrobacterium'', a genus of Gram-negative bacteria that cause tumors in plants. It was incorrectly synonymized with ''Agrobacterium tumefaciens'' until 2022. This species is widely found in soil, in plant rhizospheres, and in human clinical specimens. Some members such as B6 harbor a tumor-inducing (Ti) plasmid and cause crown gall disease in plants. The type strain ATCC 19358 does not carry such a plasmid and is nonpathogenic. The incorrect synonimization with ''A. tumefaciens'' was due to a strain B6 (ATCC 23308) being assigned as the type strain of ''A. tumefaciens'' in 1980, when B6 should be classified as ''A. radiobacter''. One consequence is that this B6 strain is very well-studied in the lab. Another consequence is that research done on "''A. tumefaciens''" between 1980 and 2022 are possibly describing ''A. radiobacter'' instead. To determine the actual species, one should look up the 16S or genomic sequenc ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Legume
Legumes are plants in the pea family Fabaceae (or Leguminosae), or the fruit or seeds of such plants. When used as a dry grain for human consumption, the seeds are also called pulses. Legumes are grown agriculturally, primarily for human consumption, but also as livestock forage and silage, and as soil-enhancing green manure. Legumes produce a botanically unique type of fruit – a simple fruit, simple Dry fruits, dry fruit that develops from a simple carpel and usually Dehiscence (botany) , dehisces (opens along a seam) on two sides. Most legumes have Symbiosis , symbiotic nitrogen fixation , nitrogen-fixing bacteria, Rhizobia, in structures called root nodules. Some of the fixed nitrogen becomes available to later crops, so legumes play a key role in crop rotation. Terminology The term ''pulse'', as used by the United Nations' Food and Agriculture Organization (FAO), is reserved for legume crops harvested solely for the dry seed. This excludes green beans and Pea , green ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Virulent
Virulence is a pathogen's or microorganism's ability to cause damage to a host. In most cases, especially in animal systems, virulence refers to the degree of damage caused by a microbe to its host. The pathogenicity of an organism—its ability to cause disease—is determined by its virulence factors. In the specific context of gene for gene systems, often in plants, virulence refers to a pathogen's ability to infect a resistant host. Virulence can also be transferred using a plasmid. The noun ''virulence'' (Latin noun ) derives from the adjective ''virulent'', meaning disease severity. The word ''virulent'' derives from the Latin word ''virulentus'', meaning "a poisoned wound" or "full of poison". The term ''virulence'' does not only apply to viruses. From an ecological standpoint, virulence is the loss of fitness induced by a parasite upon its host. Virulence can be understood in terms of proximate causes—those specific traits of the pathogen that help make the host il ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Current Protocols In Microbiology
''Current Protocols'' is a series of laboratory manuals for life scientists. The first title, ''Current Protocols in Molecular Biology'', was established in 1987 by the founding editors Frederick M. Ausubel, Roger Brent, Robert Kingston, David Moore, Jon Seidman, Kevin Struhl, and John A. Smith of the Massachusetts General Hospital Department of Molecular Biology and the Harvard Medical School Departments of Genetics and Biological Chemistry, and Sarah Greene of Greene Publishing AssociatesAusubel, F. M., Brent, R., Kingston, R., Moore, D. D., Seiman, J., Smith, J. A., and Struhl, K. (1987-2016). ''Current Protocols in Molecular Biology''. New York, NY, John Wiley & Sons, . The Current Protocols series entered into a partnership with Wiley-Interscience, John Wiley and Sons, was acquired by Wiley in 1995, and continued to introduce additional titles. Scientists contribute methods that are peer-reviewed by one of 18 editorial board The editorial board is a group of editors, writers, ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Missouri Botanical Garden
The Missouri Botanical Garden is a botanical garden located at 4344 Shaw Boulevard in St. Louis, Missouri. It is also known informally as Shaw's Garden for founder and philanthropy, philanthropist Henry Shaw (philanthropist), Henry Shaw. Its herbarium, with more than 6.6 million specimens, is the second largest in North America, behind that of the New York Botanical Garden. Its Peter H. Raven Library contains 85% coverage of all literature ever published on systematic botany and plant taxonomy. The ''Index Herbariorum'' code assigned to the herbarium is MO and it is used when citing housed specimens. History The land that is currently the Missouri Botanical Garden was previously the land of businessman Henry Shaw. Founded in 1859, the Missouri Botanical Garden is one of the oldest botanical institutions in the United States and a National Historic Landmark. It is also listed in the National Register of Historic Places. In 1983, the botanical garden was added as the fourt ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Rhubarb
Rhubarb is the fleshy, edible stalks ( petioles) of species and hybrids (culinary rhubarb) of ''Rheum'' in the family Polygonaceae, which are cooked and used for food. The plant is a herbaceous perennial that grows from short, thick rhizomes. Historically, different plants have been called "rhubarb" in English. The large, triangular leaves contain high levels of oxalic acid and anthrone glycosides, making them inedible. The small flowers are grouped in large compound leafy greenish-white to rose-red inflorescences. The precise origin of culinary rhubarb is unknown. The species '' Rheum rhabarbarum'' (syn. ''R. undulatum'') and '' R. rhaponticum'' were grown in Europe before the 18th century and used for medicinal purposes. By the early 18th century, these two species and a possible hybrid of unknown origin, ''R.'' × ''hybridum'', were grown as vegetable crops in England and Scandinavia. They readily hybridize, and culinary rhubarb was developed by selecti ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Horse Radish
Horseradish (''Armoracia rusticana'', syn. ''Cochlearia armoracia'') is a perennial plant of the family Brassicaceae (which also includes mustard, wasabi, broccoli, cabbage, and radish). It is a root vegetable, cultivated and used worldwide as a spice and as a condiment. The species is probably native to Southeastern Europe and Western Asia. Description Horseradish grows up to tall, with hairless bright green unlobed leaves up to long that may be mistaken for docks (''Rumex''). It is cultivated primarily for its large, white, tapered root. The white four-petalled flowers are scented and are borne in dense panicles. Established plants may form extensive patches and may become invasive unless carefully managed. Intact horseradish root has little aroma. When cut or grated, enzymes from within the plant cells digest sinigrin (a glucosinolate) to produce allyl isothiocyanate (mustard oil), which irritates the mucous membranes of the sinuses and eyes. Once exposed to air or heat ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Sugar Beets
A sugar beet is a plant whose root contains a high concentration of sucrose and that is grown commercially for sugar production. In plant breeding, it is known as the Altissima cultivar group of the common beet (''Beta vulgaris''). Together with other beet cultivars, such as beetroot and chard, it belongs to the subspecies ''Beta vulgaris'' subsp. ''vulgaris'' but classified as ''var. saccharifera''. Its closest wild relative is the sea beet (''Beta vulgaris'' subsp. ''maritima''). Sugar beets are grown in climates that are too cold for sugarcane. In 2020, Russia, the United States, Germany, France and Turkey were the world's five largest sugar beet producers. In 2010–2011, Europe, and North America except Arctic territories failed to supply the overall domestic demand for sugar and were all net importers of sugar. The US harvested of sugar beets in 2008. In 2009, sugar beets accounted for 20% of the world's sugar production and nearly 30% by 2013. Sugarcane accounts for most ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Nut (fruit)
A nut is a fruit consisting of a hard or tough nutshell protecting a kernel which is usually edible. In general usage and in a culinary sense, many dry seeds are called nuts, but in a botanical context, "nut" implies that the shell does not open to release the seed (Dehiscence (botany), indehiscent). Most seeds come from fruits that naturally free themselves from the shell, but this is not the case in nuts such as hazelnuts, chestnuts, and acorns, which have hard shell walls and originate from a compound ovary. Definition A seed is the mature fertilised ovule of a plant; it consists of three parts, the embryo which will develop into a new plant, stored food for the embryo, and a protective seed coat. Botany, Botanically, a nut is a fruit with a woody pericarp developing from a syncarpous gynoecium. Nuts may be contained in an Bract#Involucral bracts, involucre, a cup-shaped structure formed from the flower bracts. The involucre may be scaly, spiny, leafy or tubular, depending ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Stone Fruit
In botany, a drupe (or stone fruit) is a type of fruit in which an outer fleshy part (exocarp, or skin, and mesocarp, or flesh) surrounds a single shell (the ''pip'' (UK), ''pit'' (US), ''stone'', or ''pyrena'') of hardened endocarp with a seed (''kernel'') inside. Drupes do not split open to release the seed, i.e., they are indehiscent. These fruits usually develop from a single carpel, and mostly from flowers with superior ovaries ( polypyrenous drupes are exceptions). The definitive characteristic of a drupe is that the hard, woody ( lignified) stone is derived from the ovary wall of the flower. In an aggregate fruit, which is composed of small, individual drupes (such as a raspberry), each individual is termed a drupelet, and may together form an aggregate fruit. Such fruits are often termed ''berries'', although botanists use a different definition of ''berry''. Other fleshy fruits may have a stony enclosure that comes from the seed coat surrounding the seed, but such f ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Grape Vine
''Vitis'' (grapevine) is a genus of 81 accepted species of vining plants in the flowering plant family Vitaceae. The genus consists of species predominantly from the Northern Hemisphere. It is economically important as the source of grapes, both for direct consumption of the fruit and for fermentation to produce wine. The study and cultivation of grapevines is called viticulture. Most cultivated ''Vitis'' varieties are wind-pollinated with hermaphroditic flowers containing both male and female reproductive structures, while wild species are dioecious. These flowers are grouped in bunches called inflorescences. In many species, such as ''Vitis vinifera'', each successfully pollinated flower becomes a grape berry with the inflorescence turning into a cluster of grapes. While the flowers of the grapevines are usually very small, the berries are often large and brightly colored with sweet flavors that attract birds and other animals to disperse the seeds contained within the berri ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Walnuts
A walnut is the edible seed of any tree of the genus ''Juglans'' (family Juglandaceae), particularly the Persian or English walnut, '' Juglans regia''. They are accessory fruit because the outer covering of the fruit is technically an involucre and thus not morphologically part of the carpel; this means it cannot be a drupe but is instead a drupe-like nut. After full ripening, the shell is discarded, and the kernel is eaten. Nuts of the eastern black walnut ('' Juglans nigra'') and butternuts ('' Juglans cinerea'') are less commonly consumed. Description Walnuts are the round, single-seed stone fruits of the walnut tree. They ripen between September and November in the northern hemisphere. The brown, wrinkly walnut shell is enclosed in a husk. Shells of walnuts available in commerce usually have two segments (but three or four-segment shells can also form). During the bumming process, the husk becomes brittle and the shell hard. The shell encloses the kernel or meat, wh ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |