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(3-(2-furoyl)-quinoline-2 Carboxaldehyde)
3-(2-Furoyl)-quinoline-2-carboxaldehyde (FQ) is a fluorogenic amine labeling dye that is not fluorescent itself, but reacts with primary amines to form fluorescent products. It was first reported in 1990. Cyanide, typically provided via KCN or NaCN salts, is a required co-substrate in the fluorogenic reaction. It has been used for the detection of amines and peptides, largely in CE-SDS, where it is recognized to reach a silver stain-like high sensitivity via laser-induced fluorescence. Once bound to protein the excitation wavelength is 480 nm (blue) and the emission wavelength is ~600 nm (orange). Reaction See also * CBQCA * Fluorescamine Fluorescamine is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products, i.e. it is fluorogenic. It hence has been used as a reagent for the detection of amines and peptide Peptide ... * Py-1 References Dyes Quinolines Aldehydes Furanones {{organ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorogenic
Fluorogenic describes a property of chemical compounds which are initially not fluorescent, but become fluorescent through a chemical reaction, typically through an intermolecular covalent reaction which binds the now fluorescent compound to a target molecule. IUPAC uses a broader definition of fluorogenic, wherein an enhancement of fluorescence via a chemical reaction is not required, however in contrast to the IUPAC definition common use of fluorogenic does not refer to non-reaction effects like the enhancement of fluorescence from a fluorophore being in different solvents. Fluorogenic labeling reagents are often used in analytical chemistry procedures, particularly in HPLC or CE to derivative target compounds (e.g. labeling the primary amines of polypeptides), thereby allowing enhanced sensitivity through fluorescence based detection. Examples * OPA * Fluorescamine Fluorescamine is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Laser-induced Fluorescence
Laser-induced fluorescence (LIF) or laser-stimulated fluorescence (LSF) is a spectroscopic method in which an atom or molecule is excited to a higher energy level by the absorption of laser light followed by spontaneous emission of light. It was first reported by Zare and coworkers in 1968. LIF is used for studying structure of molecules, detection of selective species and flow visualization and measurements. The wavelength is often selected to be the one at which the species has its largest cross section. The excited species will after some time, usually in the order of few nanoseconds to microseconds, spontaneously decay and emit a photon at a wavelength longer than the excitation wavelength. This fluorescent light is typically recorded with a photomultiplier tube (PMT), charged-coupled device (CCD), or filtered photodiodes. Types Two different kinds of spectra exist, disperse spectra and excitation spectra. The disperse spectra are performed with a fixed lasing wavelength, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Quinolines
Quinoline is a heterocyclic aromatic organic compound with the chemical formula C9H7N. It is a colorless hygroscopic liquid with a strong odor. Aged samples, especially if exposed to light, become yellow and later brown. Quinoline is only slightly soluble in cold water but dissolves readily in hot water and most organic solvents. Quinoline itself has few applications, but many of its derivatives are useful in diverse applications. A prominent example is quinine, an alkaloid found in plants. Over 200 biologically active quinoline and quinazoline alkaloids are identified. 4-Hydroxy-2-alkylquinolines (HAQs) are involved in antibiotic resistance. Occurrence and isolation Quinoline was first extracted from coal tar in 1834 by German chemist Friedlieb Ferdinand Runge; he called quinoline ''leukol'' ("white oil" in Greek). Coal tar remains the principal source of commercial quinoline. In 1842, French chemist Charles Gerhardt obtained a compound by dry distilling quinine, str ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dyes
Juan de Guillebon, better known by his stage name DyE, is a French musician. He is known for the music video of the single "Fantasy (DyE song), Fantasy" from his first album ''Taki 183 (album), Taki 183''. This video became popular, attracting over 65 million views, 49 million of those within two years. Discography Albums *''Taki 183 (album), Taki 183'' (2011) *''Cocktail Citron'' (2014) *''Inside Out (2018)'' *''MySpace (2024)'' EPs *''Imperator'' (2009) *''Emo Machine'' (2017) References External linksDyE at Myspace French musicians Living people Year of birth missing (living people) {{France-musician-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pyrylium-1
Pyrylium-1 (Py-1) is a fluorogenic salt of a pyrylium derivative and tetrafluoroborate. It is an amine-labeling dye that is not fluorescent itself, but reacts with primary amines to form fluorescent products. It is within the "chameleon labels" class, so named due to their clear color-changing properties upon conjugation. Py-1 was first reported in 2004. It has been used for the detection of amines and peptides, largely in CE-SDS, where it is recognized to reach a high sensitivity via laser-induced fluorescence. Once bound to protein the excitation wavelength is 503 nm (green) and the emission wavelength is 603 nm (orange). Similar to FQ, these fluorescence wavelengths makes Py-1 suitable for excitation with a 488 nm argon-ion laser. Reaction Post conjugation, the Py-1 adduct (an addition of C21H21N) adds about 287.1674 Da to the target molecule. See also * FQ * CBQCA * Fluorescamine Fluorescamine is a spiro compound that is not fluorescent itself, but reacts with pri ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescamine
Fluorescamine is a spiro compound that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products, i.e. it is fluorogenic. It hence has been used as a reagent for the detection of amines and peptide Peptides are short chains of amino acids linked by peptide bonds. A polypeptide is a longer, continuous, unbranched peptide chain. Polypeptides that have a molecular mass of 10,000 Da or more are called proteins. Chains of fewer than twenty am ...s. 1-100 μg of protein and down to 10 pg of protein can be detected. Once bound to protein the excitation wavelength is 381 nm (near ultraviolet) and the emission wavelength is 470 nm (blue). This method is found to suffer from high blanks resulting from a high rate of hydrolysis due to requiring a large excess concentration. Alternative methods are based on Phthalaldehyde, ''ortho''-phthalaldehyde (OPA), Ellman's reagent (DTNB), or epicocconone. Reaction : See also * FQ References Lacto ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
CBQCA
3-(4-Carboxybenzoyl)quinoline-2-carboxaldehyde (CBQCA) is a fluorogenic amine labeling dye that is not fluorescent itself, but covalently reacts with primary amines to form fluorescent products. It was first reported in 1991. CBQCA is largely used in the context of quantifying peptides or proteins. Either cyanide or thiols In organic chemistry, a thiol (; ), or thiol derivative, is any organosulfur compound of the form , where R represents an alkyl or other organic substituent. The functional group itself is referred to as either a thiol group or a sulfhydryl grou ... are required as a co-substrate in the fluorogenic reaction, although thiols also react with and mask the CBQCA aldehyde thereby preventing the fluorogenic reaction against the targeted primary amines. Once bound to protein the excitation wavelength is 465 nm (blue) and the emission wavelength is ~550 nm (green). Reaction CBQCA, which is non-fluorescent, reacts with primary amines in the presence of cyanide to f ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
FQ Reaction Scheme , a fluorogenic amine labeling dye
{{disambig ...
FQ, Fq or fq may refer to: * Fenqing, a Chinese term for "angry youth" * Financial Quotient, a measure of financial ability * Fluoroquinolone, an antibiotic * Football Queensland, an Australian sports organisation * Baker Island (FIPS PUB 10-4 territory code FQ), an unincorporated and unorganized territory of the U.S. * Thomas Cook Airlines Belgium (former IATA code FQ), a Belgian leisure airline * (3-(2-furoyl)-quinoline-2 carboxaldehyde) 3-(2-Furoyl)-quinoline-2-carboxaldehyde (FQ) is a fluorogenic amine labeling dye that is not fluorescent itself, but reacts with primary amines to form fluorescent products. It was first reported in 1990. Cyanide, typically provided via KCN or ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Silver Staining
In pathology, silver staining is the use of silver to selectively alter the appearance of a target in microscopy of histological sections; in temperature gradient gel electrophoresis; and in polyacrylamide gels. In traditional stained glass, silver stain is a technique to produce yellow to orange or brown shades (or green on a blue glass base), by adding a mixture containing silver compounds (notably silver nitrate), and firing lightly. It was introduced soon after 1800, and is the "stain" in the term "stained glass". Silver compounds are mixed with binding substances, applied to the surface of glass, and then fired in a furnace or kiln. History Camillo Golgi perfected silver staining for the study of the nervous system. Although the exact chemical mechanism by which this occurs is unknown, Golgi's method stains a limited number of cells at random in their entirety. Silver staining was introduced by Kerenyi and Gallyas as a sensitive procedure to detect trace amounts of proteins ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Primary Amines
In chemistry, amines (, ) are organic compounds that contain carbon-nitrogen bonds. Amines are formed when one or more hydrogen atoms in ammonia are replaced by alkyl or aryl groups. The nitrogen atom in an amine possesses a lone pair of electrons. Amines can also exist as hetero cyclic compounds. Aniline is the simplest aromatic amine, consisting of a benzene ring bonded to an amino group. Amines are classified into three types: primary (1°), secondary (2°), and tertiary (3°) amines. Primary amines (1°) contain one alkyl or aryl substituent and have the general formula RNH2. Secondary amines (2°) have two alkyl or aryl groups attached to the nitrogen atom, with the general formula R2NH. Tertiary amines (3°) contain three substituent groups bonded to the nitrogen atom, and are represented by the formula R3N. The functional group present in primary amines is called the amino group. Classification of amines Amines can be classified according to the nature and number o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Peptide
Peptides are short chains of amino acids linked by peptide bonds. A polypeptide is a longer, continuous, unbranched peptide chain. Polypeptides that have a molecular mass of 10,000 Da or more are called proteins. Chains of fewer than twenty amino acids are called oligopeptides, and include dipeptides, tripeptides, and tetrapeptides. Peptides fall under the broad chemical classes of biological polymers and oligomers, alongside nucleic acids, oligosaccharides, polysaccharides, and others. Proteins consist of one or more polypeptides arranged in a biologically functional way, often bound to ligands such as coenzymes and cofactors, to another protein or other macromolecule such as DNA or RNA, or to complex macromolecular assemblies. Amino acids that have been incorporated into peptides are termed residues. A water molecule is released during formation of each amide bond.. All peptides except cyclic peptides have an N-terminal (amine group) and C-terminal (carboxyl g ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
