Fluorescamine is a

spiro compound In organic chemistry, spiro compounds are compounds that have at least two molecular rings with only one common atom. The simplest spiro compounds are bicyclic (having just two rings), or have a bicyclic portion as part of the larger ring sy ...

that is not fluorescent itself, but reacts with primary amines to form highly fluorescent products. It hence has been used as a reagent for the detection of

amine In chemistry, amines (, ) are compounds and functional groups that contain a basic nitrogen atom with a lone pair. Amines are formally derivatives of ammonia (), wherein one or more hydrogen atoms have been replaced by a substituent su ...

s and

peptide Peptides (, ) are short chains of amino acids linked by peptide bonds. Long chains of amino acids are called proteins. Chains of fewer than twenty amino acids are called oligopeptides, and include dipeptides, tripeptides, and tetrapeptides. ...

s. 1-100 µg of protein and down 10 pg protein can be detected.protocol
by

Fluoprobes The FluoProbes series of fluorescent dyes were developed by Interchim to improve performances of standard fluorophores. They are designed for labeling biomolecules, cells, tissues or beads in advanced fluorescent detection techniques. * FluoProbe ...

This method is found to suffer of high blanks resulting of high rate of hydrolysis due to used excess concentration. Alternative methods are based on ortho-Phthalaldehyde (OPA), Ellman's reagent (DTNB) and

epicocconone Epicocconone is a long Stokes' shift fluorescent dye found in the fungus '' Epicoccum nigrum''. Though weakly fluorescent in water (green emission, 520 nm) it reacts reversibly with proteins to yield a product with a strong orange-red emiss ...

References

Lactones Spiro compounds {{organic-compound-stub