Second Generation Multiplex is a

DNA profiling DNA profiling (also called DNA fingerprinting and genetic fingerprinting) is the process of determining an individual's deoxyribonucleic acid (DNA) characteristics. DNA analysis intended to identify a species, rather than an individual, is cal ...

system used in the United Kingdom to set up the UK National DNA Database in 1995. It is manufactured by ABI (Applied Biosystems). It contains primers for the following STR ( Short Tandem Repeat) loci: VWA (HUMVWF31/A), D8 (D8S1179), D21 (D21S11), D18 (D18S51), THO (HUMTHO1), and FGA (HUMFIBRA). It also contains primers for the

Amelogenin Amelogenins are a group of protein isoforms produced by alternative splicing or proteolysis from the '' AMELX'' gene, on the X chromosome, and also the '' AMELY'' gene in males, on the Y chromosome. They are involved in amelogenesis, the develo ...

sex indicating test. The primers are tagged with the following

fluorescent dyes A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with sev ...

for detection under electrophoresis: 5-FAM, JOE, and NED. Its use in the United kingdom as the DNA profiling system used by The UK National DNA Database was superseded by the Second Generation Multiplex Plus SGM+ DNA profiling system in 1998.

References

{{reflist Biometrics DNA profiling techniques Law enforcement in the United Kingdom Population genetics in the United Kingdom 1995 establishments in the United Kingdom