R-banding is a
cytogenetics technique that produces the reverse of the
G-band stain on
chromosomes. R-banding is obtained by incubating the slides in hot phosphate buffer, then a subsequent treatment of
giemsa dye. Resulting chromosome patterns shows darkly stained R bands, the complement to G-bands. Darkly colored R bands are
guanine-cytosine rich, and
adenine-
thymine rich regions are more readily denatured by heat. The technique is useful for analyzing
genetic deletions
In genetics, a deletion (also called gene deletion, deficiency, or deletion mutation) (sign: delta (letter), Δ) is a mutation (a genetic aberration) in which a part of a chromosome or a sequence of DNA is left out during DNA replication. Any numb ...
or
chromosomal translocations that involve the telomeres of
chromosomes.
{{DEFAULTSORT:R-banding
Cytogenetics