''Rhizobium binae'' is a

gram-negative bacterium Gram-negative bacteria are bacteria that do not retain the crystal violet stain used in the Gram staining method of bacterial differentiation. They are characterized by their cell envelopes, which are composed of a thin peptidoglycan cell wall ...

which was isolated from

root nodules Root nodules are found on the roots of plants, primarily legumes, that form a symbiosis with nitrogen-fixing bacteria. Under nitrogen-limiting conditions, capable plants form a symbiotic relationship with a host-specific strain of bacteria known a ...

lentil The lentil (''Lens culinaris'' or ''Lens esculenta'') is an edible legume. It is an annual plant known for its lens-shaped seeds. It is about tall, and the seeds grow in pods, usually with two seeds in each. As a food crop, the largest pro ...

s in

Bangladesh Bangladesh (}, ), officially the People's Republic of Bangladesh, is a country in South Asia. It is the eighth-most populous country in the world, with a population exceeding 165 million people in an area of . Bangladesh is among the mos ...

Description

''Rhizobium binae'' are

rod-shaped A bacillus (), also called a bacilliform bacterium or often just a rod (when the context makes the sense clear), is a rod-shaped bacterium or archaeon. Bacilli are found in many different taxonomic groups of bacteria. However, the name ''Bacillu ...

bacteria found in the soil. They require oxygen and do not form spores. ''Rhizobium binae'' grow well on YEMA medium

agar Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from ogonori (''Gracilaria'') and "tengusa" (''Gelidiaceae''). As found in nature, agar is ...

, where they form

colonies In modern parlance, a colony is a territory subject to a form of foreign rule. Though dominated by the foreign colonizers, colonies remain separate from the administration of the original country of the colonizers, the '' metropolitan state'' ...

which are circular, convex and creamy white. Strains survive at pH values between 5.5 and 10. They are very sensitive to

ampicillin Ampicillin is an antibiotic used to prevent and treat a number of bacterial infections, such as respiratory tract infections, urinary tract infections, meningitis, salmonellosis, and endocarditis. It may also be used to prevent group B strepto ...

and resistant to kanamycin and nalidixic acid. Strains do not tolerate

tetracycline Tetracycline, sold under various brand names, is an oral antibiotic in the tetracyclines family of medications, used to treat a number of infections, including Acne vulgaris, acne, cholera, brucellosis, plague (disease), plague, malaria, and sy ...

and do not grow on LB medium. ''Rhizobium binae'' can utilize a variety of nutrients, including dextrin, D-maltose, D-trehalose, D-cellobiose,

gentiobiose Gentiobiose is a disaccharide composed of two units of D-glucose joined with a β(1->6) linkage. It is a white crystalline solid that is soluble in water or hot methanol. Gentiobiose is incorporated into the chemical structure of crocin, the che ...

sucrose Sucrose, a disaccharide, is a sugar composed of glucose and fructose subunits. It is produced naturally in plants and is the main constituent of white sugar. It has the molecular formula . For human consumption, sucrose is extracted and refined ...

, D-raffinose, α-D-glucose,

D-turanose Turanose is a reducing disaccharide. The -isomer is naturally occurring. Its systematic name is α--glucopyranosyl-(1→3)-α--fructofuranose. It is an analog of sucrose not metabolized by higher plants, but rather acquired through the action ...

, α-D lactose,

D-fructose Fructose, or fruit sugar, is a ketonic simple sugar found in many plants, where it is often bonded to glucose to form the disaccharide sucrose. It is one of the three dietary monosaccharides, along with glucose and galactose, that are absorbed ...

, D-melibiose, β-methyl-D-glucoside,

salicin Salicin is an alcoholic β-glucoside. Salicin is produced in (and named after) willow (''Salix'') bark. It is a biosynthetic precursor to salicylaldehyde. Medicinal aspects Salicin is found in the bark of and leaves of willows, poplars and va ...

, N-acetyl-D-galactosamine,

D-mannose Mannose is a sugar monomer of the aldohexose series of carbohydrates. It is a C-2 epimer of glucose. Mannose is important in human metabolism, especially in the glycosylation of certain proteins. Several congenital disorders of glycosylation are ...

, D-galactose,

D-mannitol Mannitol is a type of sugar alcohol used as a sweetener and medication. It is used as a low calorie sweetener as it is poorly absorbed by the intestines. As a medication, it is used to decrease pressure in the eyes, as in glaucoma, and to lower ...

, D-sorrbitol, D-arabitol,

glycerol Glycerol (), also called glycerine in British English and glycerin in American English, is a simple triol compound. It is a colorless, odorless, viscous liquid that is sweet-tasting and non-toxic. The glycerol backbone is found in lipids known ...

, D-glucose-6-phosphate, D-fructose-6-phosphate,

D-alanine Alanine (symbol Ala or A), or α-alanine, is an α-amino acid that is used in the biosynthesis of proteins. It contains an amine group and a carboxylic acid group, both attached to the central carbon atom which also carries a methyl group side c ...

L-aspartic acid Aspartic acid (symbol Asp or D; the ionic form is known as aspartate), is an α-amino acid that is used in the biosynthesis of proteins. Like all other amino acids, it contains an amino group and a carboxylic acid. Its α-amino group is in the pro ...

L-histidine Histidine (symbol His or H) is an essential amino acid that is used in the biosynthesis of proteins. It contains an α-amino group (which is in the protonated –NH3+ form under biological conditions), a carboxylic acid group (which is in the d ...

, l-pyroglutamic acid,

quinic acid Quinic acid is a cyclitol, a cyclic polyol, and a cyclohexanecarboxylic acid. It is a colorless solid that can be extracted from plant sources. Quinic acid is implicated in the perceived acidity of coffee. Occurrence and preparation The compound ...

, D-saccharic acid, methyl pyruvate, L-lactic acid,

citric acid Citric acid is an organic compound with the chemical formula HOC(CO2H)(CH2CO2H)2. It is a colorless weak organic acid. It occurs naturally in citrus fruits. In biochemistry, it is an intermediate in the citric acid cycle, which occurs in t ...

, D-malic acid, L-malic acid, bromo-succinic acid, β-hydroxy-d,l-butyric acid and

acetic acid Acetic acid , systematically named ethanoic acid , is an acidic, colourless liquid and organic compound with the chemical formula (also written as , , or ). Vinegar is at least 4% acetic acid by volume, making acetic acid the main component ...

. ''R. binae'' can not use the nutrients N-acetyle-D-mannosamine, 3-methyle glucose, inosine, glycyl-L-proline,

L-arginine Arginine is the amino acid with the formula (H2N)(HN)CN(H)(CH2)3CH(NH2)CO2H. The molecule features a guanidino group appended to a standard amino acid framework. At physiological pH, the carboxylic acid is deprotonated (−CO2−) and both the am ...

D-galacturonic acid -Galacturonic acid is a sugar acid, an oxidized form of -galactose. It is the main component of pectin, in which it exists as the polymer polygalacturonic acid.Debra Mohnen "Pectin structure and biosynthesis" Current Opinion in Plant Biology 20 ...

D-glucuronic acid Glucuronic acid (from Greek γλεῦκος "''wine, must''" and οὖρον "''urine''") is a uronic acid that was first isolated from urine (hence the name). It is found in many gums such as gum arabic (c. 18%), xanthan, and kombucha tea and ...

, glucuronamide, p-hydroxy-phenylacetic acid, D-lactic acid methyl ester, α-keto-glutaric acid,

tween 40 Polysorbates are a class of emulsifiers used in some pharmaceuticals and food preparation. They are commonly used in oral and topical pharmaceutical dosage forms. They are also often used in cosmetics to solubilize essential oils into water-based ...

propionic acid Propionic acid (, from the Greek words πρῶτος : ''prōtos'', meaning "first", and πίων : ''píōn'', meaning "fat"; also known as propanoic acid) is a naturally occurring carboxylic acid with chemical formula CH3CH2CO2H. It is a liq ...

formic acid Formic acid (), systematically named methanoic acid, is the simplest carboxylic acid, and has the chemical formula HCOOH and structure . It is an important intermediate in chemical synthesis and occurs naturally, most notably in some ants. Es ...

. ''Rhizobium binae'' can grow in the presence of the antibiotic compounds lincomycin and

potassium tellurite Potassium tellurite, K2TeO3, is an inorganic potassium-tellurium Tellurium is a chemical element with the symbol Te and atomic number 52. It is a brittle, mildly toxic, rare, silver-white metalloid. Tellurium is chemically related to seleniu ...

, but not in the presence of 1%

sodium lactate Sodium lactate is the sodium salt of lactic acid, and has a mild saline taste. It is produced by fermentation of a sugar source, such as corn or beets, and then, by neutralizing the resulting lactic acid to create a compound having the formula NaC ...

troleandomycin Troleandomycin (TAO for short) is a macrolide antibiotic. It was sold in Italy (branded Triocetin) and Turkey (branded Tekmisin). It is no longer sold in Italy as of 2018. The drug's mode of action is to bind to the ribosome, specifically in th ...

, lithium chloride or

sodium butyrate Sodium butyrate is a compound with formula Na(C3H7COO). It is the sodium salt of butyric acid. It has various effects on cultured mammalian cells including inhibition of proliferation, induction of differentiation and induction or repression of ...

. The type strain of ''R. binae'' is strain BLR195T (=LMG 28443T = DSM 29288T).

Applications

Different strains of this species can form effective nodules and enhance growth of lentil, peas and lathyrus, and are useful for bio-fertilizer production.

Genetics

''Rhizobiu binae'' is genetically very similar to its close relatives ''

Rhizobium etli ''Rhizobium etli'' is a Gram-negative root-nodule bacterium Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorgani ...

'' and '' Rhizobum phaseoli''. The

GC-content In molecular biology and genetics, GC-content (or guanine-cytosine content) is the percentage of nitrogenous bases in a DNA or RNA molecule that are either guanine (G) or cytosine (C). This measure indicates the proportion of G and C bases out o ...

of the DNA of the type strain of ''R. binae'' is 61.5%. The genome sequence is available in

NCBI The National Center for Biotechnology Information (NCBI) is part of the United States National Library of Medicine (NLM), a branch of the National Institutes of Health (NIH). It is approved and funded by the government of the United States. The ...

and the

European nucleotide archive The European Nucleotide Archive (ENA) is a repository providing free and unrestricted access to annotated DNA and RNA sequences. It also stores complementary information such as experimental procedures, details of sequence assembly and other me ...

History

''Rhizobium binae'' was first described in 2015 by M. Harun-or Rashid and others. It was isolated from the root nodules of ''

Lens culinaris The lentil (''Lens culinaris'' or ''Lens esculenta'') is an edible legume. It is an annual plant known for its lens-shaped seeds. It is about tall, and the seeds grow in pods, usually with two seeds in each. As a food crop, the largest pro ...

'' in the Feni district of Bangladesh. It was named "binae" as an abbreviation for

Bangladesh Institute of Nuclear Agriculture Bangladesh Institute of Nuclear Agriculture (BINA) is a research institute in Mymensingh, specialized in using nuclear and radiation technology in agricultural research. History BINA was established in 1961 by the Pakistan Atomic Energy Commis ...

, the research institute where the bacteria was originally studied.

References

External links