OLIGO Primer Analysis Software is a

software Software is a set of computer programs and associated software documentation, documentation and data (computing), data. This is in contrast to Computer hardware, hardware, from which the system is built and which actually performs the work. ...

for DNA primer design. The first paper describing this software was published in 1989. The program is a real time

PCR primer PCR or pcr may refer to: Science * Phosphocreatine, a phosphorylated creatine molecule * Principal component regression, a statistical technique Medicine * Polymerase chain reaction ** COVID-19 testing, often performed using the polymerase chai ...

and probe search and analysis tool, in addition to

siRNA Small interfering RNA (siRNA), sometimes known as short interfering RNA or silencing RNA, is a class of double-stranded RNA at first non-coding RNA molecules, typically 20-24 (normally 21) base pairs in length, similar to miRNA, and operating ...

and molecular beacon searches,

open reading frame In molecular biology, open reading frames (ORFs) are defined as spans of DNA sequence between the start and stop codons. Usually, this is considered within a studied region of a Prokaryote, prokaryotic DNA sequence, where only one of the #Six-fra ...

restriction enzyme A restriction enzyme, restriction endonuclease, REase, ENase or'' restrictase '' is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class ...

analysis. It was created and maintained by Wojciech Rychlik and Piotr Rychlik. OLIGO Primer Analysis Software has been used for: real time PCR, apoptosis studies, antigen typing, species identification, studies on species evolution, measuring mRNA expression levels, oligonucleotide-based array hybridization studies, degenerate primer studies, microsatellite analysis, DNA microarray detection, inverse PCR, genome walking, nucleotide polymorphisms studies, detection of microorganisms or viruses, genotyping, cloning, vector (gene) construction, genome sequencing, detection of mutants or intraspecific variability, genetic disease studies, siRNA and gene silencing, FISH analysis (single cell expression study), scorpion probes, and development of new DNA amplification methods.Chunsun Zhang and Da Xing (2010) Microfluidic gradient PCR (MG-PCR): a new method for microfluidic DNA amplification; Biomedical Microdevices 12, 1-12.

References

Oligo Primer Analysis Software by Molecular Biology Insights

Other Primer Design Software

* FastPCR * Gene Designer * Geneious * MacVector *

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* Vector NTI {{DEFAULTSORT:Oligo Primer Analysis Software Bioinformatics software