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''Azadirachta indica'', commonly known as neem, margosa, nimtree or Indian lilac, is a tree in the mahogany family Meliaceae. It is one of the two species in the genus '' Azadirachta''. It is native to the
Indian subcontinent The Indian subcontinent is a physiographic region of Asia below the Himalayas which projects into the Indian Ocean between the Bay of Bengal to the east and the Arabian Sea to the west. It is now divided between Bangladesh, India, and Pakista ...
and to parts of
Southeast Asia Southeast Asia is the geographical United Nations geoscheme for Asia#South-eastern Asia, southeastern region of Asia, consisting of the regions that are situated south of China, east of the Indian subcontinent, and northwest of the Mainland Au ...
, but is naturalized and grown around the world in
tropical The tropics are the regions of Earth surrounding the equator, where the sun may shine directly overhead. This contrasts with the temperate or polar regions of Earth, where the Sun can never be directly overhead. This is because of Earth's ax ...
and
subtropical The subtropical zones or subtropics are geographical zone, geographical and Köppen climate classification, climate zones immediately to the Northern Hemisphere, north and Southern Hemisphere, south of the tropics. Geographically part of the Ge ...
areas. Its fruits and seeds are the source of
neem oil Neem oil, also known as margosa oil, is a vegetable oil pressed from the fruits and seeds of the neem (''Azadirachta indica''), a tree which is indigenous to the Indian subcontinent and has been introduced to many other areas in the tropics. It ...
. ''Nim'' is a Hindustani noun derived from
Sanskrit Sanskrit (; stem form ; nominal singular , ,) is a classical language belonging to the Indo-Aryan languages, Indo-Aryan branch of the Indo-European languages. It arose in northwest South Asia after its predecessor languages had Trans-cultural ...
''nimba'' ().


Description

Margosa is a fast-growing
tree In botany, a tree is a perennial plant with an elongated stem, or trunk, usually supporting branches and leaves. In some usages, the definition of a tree may be narrower, e.g., including only woody plants with secondary growth, only ...
that can reach a height of , and rarely . It is
evergreen In botany, an evergreen is a plant which has Leaf, foliage that remains green and functional throughout the year. This contrasts with deciduous plants, which lose their foliage completely during the winter or dry season. Consisting of many diffe ...
, shedding many of its leaves during the dry winter months. The branches are wide and spreading. The fairly dense crown is roundish and may reach a diameter of . The opposite, pinnate leaves are long, with 20 to 30 medium to dark green leaflets about long. The terminal leaflet often is missing. The petioles are short. White and fragrant
flower Flowers, also known as blooms and blossoms, are the reproductive structures of flowering plants ( angiosperms). Typically, they are structured in four circular levels, called whorls, around the end of a stalk. These whorls include: calyx, m ...
s are arranged in more-or-less drooping axillary
panicle In botany, a panicle is a much-branched inflorescence. (softcover ). Some authors distinguish it from a compound spike inflorescence, by requiring that the flowers (and fruit) be pedicellate (having a single stem per flower). The branches of a p ...
s which are up to long. The
inflorescence In botany, an inflorescence is a group or cluster of flowers arranged on a plant's Plant stem, stem that is composed of a main branch or a system of branches. An inflorescence is categorized on the basis of the arrangement of flowers on a mai ...
s, which branch up to the third degree, bear from 250 to 300 flowers. An individual flower is long and wide. Protandrous, bisexual flowers and male flowers exist on the same individual tree. The
fruit In botany, a fruit is the seed-bearing structure in flowering plants (angiosperms) that is formed from the ovary after flowering. Fruits are the means by which angiosperms disseminate their seeds. Edible fruits in particular have long propaga ...
is a smooth ( glabrous), olive-like
drupe In botany, a drupe (or stone fruit) is a type of fruit in which an outer fleshy part (exocarp, or skin, and mesocarp, or flesh) surrounds a single shell (the ''pip'' (UK), ''pit'' (US), ''stone'', or ''pyrena'') of hardened endocarp with a seed ...
which varies in shape from elongate oval to nearly roundish, and when ripe is by . The fruit skin (exocarp) is thin and the bitter-sweet pulp (mesocarp) is yellowish-white and very fibrous. The mesocarp is thick. The white, hard inner shell (endocarp) of the fruit encloses one, rarely two, or three, elongated
seed In botany, a seed is a plant structure containing an embryo and stored nutrients in a protective coat called a ''testa''. More generally, the term "seed" means anything that can be Sowing, sown, which may include seed and husk or tuber. Seeds ...
s (kernels) having a brown seed coat. The margosa tree is similar in appearance to its relative, the chinaberry or bakain, ''
Melia azedarach ''Melia azedarach'', commonly known as the chinaberry tree, pride of India, bead-tree, Cape lilac, syringa berrytree, Persian lilac, Indian lilac, or white cedar, is a species of deciduous tree in the mahogany family (biology), family, Meliace ...
'', with which it may be confused. ''Melia azedarach'' also has toothed leaflets and similar looking fruit. One difference is that margosa leaves are pinnate but chinaberry leaves are twice- and thrice-pinnate.


Taxonomy

The name ''Azadirachta indica'' was first published by Adrien-Henri de Jussieu in 1830. In 1753,
Carl Linnaeus Carl Linnaeus (23 May 1707 – 10 January 1778), also known after ennoblement in 1761 as Carl von Linné,#Blunt, Blunt (2004), p. 171. was a Swedish biologist and physician who formalised binomial nomenclature, the modern system of naming o ...
had described two species, ''
Melia azedarach ''Melia azedarach'', commonly known as the chinaberry tree, pride of India, bead-tree, Cape lilac, syringa berrytree, Persian lilac, Indian lilac, or white cedar, is a species of deciduous tree in the mahogany family (biology), family, Meliace ...
'' and ''Melia azadirachta''. De Jussieu considered ''Melia azadirachta'' to be sufficiently different from ''Melia azedarach'' to be placed in a new genus.p. 221
/ref> For both his species, Linnaeus referred to the name 'azedarach', which is derived from the French 'azédarac', which in turn is from the Persian 'āzād dirakht' (ازادرخت), meaning 'free or noble tree'. The Persian name of the tree, ''azad darakhat-e-hind'', meaning 'the free tree of India'', implies that it is free from disease and insect problems.


Distribution

''Azadirachta indica'' is considered to be native to the Assam region,
Pakistan Pakistan, officially the Islamic Republic of Pakistan, is a country in South Asia. It is the List of countries and dependencies by population, fifth-most populous country, with a population of over 241.5 million, having the Islam by country# ...
and
Bangladesh Bangladesh, officially the People's Republic of Bangladesh, is a country in South Asia. It is the List of countries and dependencies by population, eighth-most populous country in the world and among the List of countries and dependencies by ...
in the Indian subcontinent and to
Cambodia Cambodia, officially the Kingdom of Cambodia, is a country in Southeast Asia on the Mainland Southeast Asia, Indochinese Peninsula. It is bordered by Thailand to the northwest, Laos to the north, and Vietnam to the east, and has a coastline ...
,
Laos Laos, officially the Lao People's Democratic Republic (LPDR), is the only landlocked country in Southeast Asia. It is bordered by Myanmar and China to the northwest, Vietnam to the east, Cambodia to the southeast, and Thailand to the west and ...
,
Myanmar Myanmar, officially the Republic of the Union of Myanmar; and also referred to as Burma (the official English name until 1989), is a country in northwest Southeast Asia. It is the largest country by area in Mainland Southeast Asia and has ...
,
Thailand Thailand, officially the Kingdom of Thailand and historically known as Siam (the official name until 1939), is a country in Southeast Asia on the Mainland Southeast Asia, Indochinese Peninsula. With a population of almost 66 million, it spa ...
and
Vietnam Vietnam, officially the Socialist Republic of Vietnam (SRV), is a country at the eastern edge of mainland Southeast Asia, with an area of about and a population of over 100 million, making it the world's List of countries and depende ...
in Indochina. It has been widely introduced elsewhere in tropical and subtropical regions, from
South America South America is a continent entirely in the Western Hemisphere and mostly in the Southern Hemisphere, with a considerably smaller portion in the Northern Hemisphere. It can also be described as the southern Subregion#Americas, subregion o ...
to
Indonesia Indonesia, officially the Republic of Indonesia, is a country in Southeast Asia and Oceania, between the Indian Ocean, Indian and Pacific Ocean, Pacific oceans. Comprising over List of islands of Indonesia, 17,000 islands, including Sumatra, ...
.


Ecology

The margosa tree is noted for its drought resistance. Normally, it thrives in areas with sub-arid to sub-humid conditions, with an annual rainfall of . It can grow in regions with an annual rainfall below 400 mm, but in such cases it depends largely on ground water levels. Margosa can grow in many different types of
soil Soil, also commonly referred to as earth, is a mixture of organic matter, minerals, gases, water, and organisms that together support the life of plants and soil organisms. Some scientific definitions distinguish dirt from ''soil'' by re ...
, but it thrives best on well-drained deep and sandy soils. It is a typical tropical to subtropical tree and exists at annual mean temperatures of . It can tolerate high to very high temperatures and does not tolerate temperature below . Margosa is one of very few shade-giving trees that thrive in drought-prone areas such as the dry coastal, southern districts of
India India, officially the Republic of India, is a country in South Asia. It is the List of countries and dependencies by area, seventh-largest country by area; the List of countries by population (United Nations), most populous country since ...
and
Pakistan Pakistan, officially the Islamic Republic of Pakistan, is a country in South Asia. It is the List of countries and dependencies by population, fifth-most populous country, with a population of over 241.5 million, having the Islam by country# ...
. The trees are not at all delicate about water quality and thrive on the merest trickle of water, whatever the quality. In India and tropical countries where the
Indian diaspora Overseas Indians (ISO 15919, ISO: ), officially Non-Resident Indians (NRIs) and People of Indian Origin (PIOs) are people of Indian descent who reside or originate outside of India (Including those that were directly under the British Raj). Acc ...
has reached, it is very common to see margosa trees used for shade lining streets, around temples, schools and other such public buildings or in most people's backyards. In very dry areas, the trees are planted on large tracts of land.


Weed status

Margosa is considered as a
weed A weed is a plant considered undesirable in a particular situation, growing where it conflicts with human preferences, needs, or goals.Harlan, J. R., & deWet, J. M. (1965). Some thoughts about weeds. ''Economic botany'', ''19''(1), 16-24. Pla ...
in many areas, including some parts of the
Middle East The Middle East (term originally coined in English language) is a geopolitical region encompassing the Arabian Peninsula, the Levant, Turkey, Egypt, Iran, and Iraq. The term came into widespread usage by the United Kingdom and western Eur ...
, most of Sub-
Sahara The Sahara (, ) is a desert spanning across North Africa. With an area of , it is the largest hot desert in the world and the list of deserts by area, third-largest desert overall, smaller only than the deserts of Antarctica and the northern Ar ...
n
Africa Africa is the world's second-largest and second-most populous continent after Asia. At about 30.3 million km2 (11.7 million square miles) including adjacent islands, it covers 20% of Earth's land area and 6% of its total surfac ...
including
West Africa West Africa, also known as Western Africa, is the westernmost region of Africa. The United Nations geoscheme for Africa#Western Africa, United Nations defines Western Africa as the 16 countries of Benin, Burkina Faso, Cape Verde, The Gambia, Gha ...
and
Indian Ocean The Indian Ocean is the third-largest of the world's five oceanic divisions, covering or approximately 20% of the water area of Earth#Surface, Earth's surface. It is bounded by Asia to the north, Africa to the west and Australia (continent), ...
states, and some parts of
Australia Australia, officially the Commonwealth of Australia, is a country comprising mainland Australia, the mainland of the Australia (continent), Australian continent, the island of Tasmania and list of islands of Australia, numerous smaller isl ...
. Ecologically, it survives well in similar environments to its own, but its weed potential has not been fully assessed. In April 2015, ''A. indica'' was declared a class B and C weed in the
Northern Territory The Northern Territory (abbreviated as NT; known formally as the Northern Territory of Australia and informally as the Territory) is an states and territories of Australia, Australian internal territory in the central and central-northern regi ...
,
Australia Australia, officially the Commonwealth of Australia, is a country comprising mainland Australia, the mainland of the Australia (continent), Australian continent, the island of Tasmania and list of islands of Australia, numerous smaller isl ...
, meaning its growth and spread must be controlled and plants or propagules are not allowed to be brought into the territory. It is illegal to buy, sell, or transport the plants or seeds. Its declaration as a weed came in response to its invasion of waterways in the "
Top End The Top End of Australia's Northern Territory is a geographical region encompassing the northernmost section of the Northern Territory, which aside from the Cape York Peninsula is the northernmost part of the Australian continent. It covers a ...
" of the territory. After being introduced into Australia, possibly in the 1940s, ''A. indica'' was originally planted in the
Northern Territory The Northern Territory (abbreviated as NT; known formally as the Northern Territory of Australia and informally as the Territory) is an states and territories of Australia, Australian internal territory in the central and central-northern regi ...
to provide shade for cattle. Trial plantations were established between the 1960s and 1980s in Darwin,
Queensland Queensland ( , commonly abbreviated as Qld) is a States and territories of Australia, state in northeastern Australia, and is the second-largest and third-most populous state in Australia. It is bordered by the Northern Territory, South Austr ...
, and
Western Australia Western Australia (WA) is the westernmost state of Australia. It is bounded by the Indian Ocean to the north and west, the Southern Ocean to the south, the Northern Territory to the north-east, and South Australia to the south-east. Western Aust ...
, but the Australian margosa industry did not prove viable. The tree has now spread into the savanna, particularly around waterways, and naturalised populations exist in several areas.


Phytochemicals

Margosa fruit, seeds, leaves, stems, and bark contain diverse phytochemicals, some of which were first discovered in azadirachta seed
extract An extract (essence) is a substance made by extracting a part of a raw material, often by using a solvent such as ethanol, oil or water. Extracts may be sold as tinctures or absolutes or dried and powdered. The aromatic principles of ma ...
s, such as azadirachtin established in the 1960s as an insect antifeedant, growth disruptor, and
insecticide Insecticides are pesticides used to kill insects. They include ovicides and larvicides used against insect eggs and larvae, respectively. The major use of insecticides is in agriculture, but they are also used in home and garden settings, i ...
. The yield of azadirachtin from crushing 2 kg of seeds is about 5 g. In addition to azadirachtin and related limonoids, the seed oil contains glycerides, diverse
polyphenol Polyphenols () are a large family of naturally occurring phenols. They are abundant in plants and structurally diverse. Polyphenols include phenolic acids, flavonoids, tannic acid, and ellagitannin, some of which have been used historically as ...
s, nimbolide, triterpenes, and beta-sitosterol. The yellow, bitter oil has a
garlic Garlic (''Allium sativum'') is a species of bulbous flowering plants in the genus '' Allium''. Its close relatives include the onion, shallot, leek, chives, Welsh onion, and Chinese onion. Garlic is native to central and south Asia, str ...
-like odor and contains about 2% of limonoid compounds. The leaves contain quercetin,
catechin Catechin is a flavan-3-ol, a type of secondary metabolite providing antioxidant roles in plants. It belongs to the subgroup of polyphenols called flavonoids. The name of the catechin chemical family derives from ''catechu'', which is the tannic ...
s,
carotene The term carotene (also carotin, from the Latin ''carota'', "carrot") is used for many related unsaturated hydrocarbon substances having the formula C40Hx, which are synthesized by plants but in general cannot be made by animals (with the ex ...
s, and
vitamin C Vitamin C (also known as ascorbic acid and ascorbate) is a water-soluble vitamin found in citrus and other fruits, berries and vegetables. It is also a generic prescription medication and in some countries is sold as a non-prescription di ...
.


Uses

Margosa leaves are dried in India and placed in cupboards to prevent insects from eating clothes, and in containers in which
rice Rice is a cereal grain and in its Domestication, domesticated form is the staple food of over half of the world's population, particularly in Asia and Africa. Rice is the seed of the grass species ''Oryza sativa'' (Asian rice)—or, much l ...
and
wheat Wheat is a group of wild and crop domestication, domesticated Poaceae, grasses of the genus ''Triticum'' (). They are Agriculture, cultivated for their cereal grains, which are staple foods around the world. Well-known Taxonomy of wheat, whe ...
are stored. The flowers are also used in many Indian festivals like Ugadi. ''See below: #Association with Hindu festivals in India.''


Culinary

The tender shoots and flowers of the margosa tree are eaten as a vegetable in India. A soup-like dish called ' () in Tamil (translated as "margosa flower rasam") made of the flower of neem is prepared in
Tamil Nadu Tamil Nadu (; , TN) is the southernmost States and union territories of India, state of India. The List of states and union territories of India by area, tenth largest Indian state by area and the List of states and union territories of Indi ...
. In
Bengal Bengal ( ) is a Historical geography, historical geographical, ethnolinguistic and cultural term referring to a region in the Eastern South Asia, eastern part of the Indian subcontinent at the apex of the Bay of Bengal. The region of Benga ...
, young margosa leaves are fried in oil with tiny pieces of aubergine (brinjal). The dish is called ''nim bēgun bhājā'' () and is the first item during a Bengali meal, which acts as an appetizer. It is eaten with rice. Margosa is used in parts of mainland
Southeast Asia Southeast Asia is the geographical United Nations geoscheme for Asia#South-eastern Asia, southeastern region of Asia, consisting of the regions that are situated south of China, east of the Indian subcontinent, and northwest of the Mainland Au ...
, particularly in
Cambodia Cambodia, officially the Kingdom of Cambodia, is a country in Southeast Asia on the Mainland Southeast Asia, Indochinese Peninsula. It is bordered by Thailand to the northwest, Laos to the north, and Vietnam to the east, and has a coastline ...
, Laos (where it is called ''kadao'' []),
Thailand Thailand, officially the Kingdom of Thailand and historically known as Siam (the official name until 1939), is a country in Southeast Asia on the Mainland Southeast Asia, Indochinese Peninsula. With a population of almost 66 million, it spa ...
(where it is known as ''sadao'' or ''satao'' ,
Myanmar Myanmar, officially the Republic of the Union of Myanmar; and also referred to as Burma (the official English name until 1989), is a country in northwest Southeast Asia. It is the largest country by area in Mainland Southeast Asia and has ...
(where it is known as ''ta.ma'' []) and Vietnam (where it is known as and is used to cook the salad ). Even if lightly cooked, the flavour is quite bitter, and the food is not consumed by all inhabitants of these nations. In Myanmar, young margosa leaves and flower buds are boiled with
tamarind Tamarind (''Tamarindus indica'') is a Legume, leguminous tree bearing edible fruit that is indigenous to tropical Africa and naturalized in Asia. The genus ''Tamarindus'' is monotypic taxon, monotypic, meaning that it contains only this spe ...
fruit to soften its bitterness and eaten as a vegetable. Pickled margosa leaves are also eaten with tomato and fish paste sauce in Myanmar.


Traditional medicine

Products made from margosa trees have been used in the
traditional medicine Traditional medicine (also known as indigenous medicine or folk medicine) refers to the knowledge, skills, and practices rooted in the cultural beliefs of various societies, especially Indigenous groups, used for maintaining health and treatin ...
of India for centuries, for treating skin troubles and rheumatism, but there is insufficient clinical evidence to indicate any benefits of using margosa for medicinal purposes. In adults, no specific doses have been established, and short-term use of margosa appears to be safe, while long-term use may harm the
kidney In humans, the kidneys are two reddish-brown bean-shaped blood-filtering organ (anatomy), organs that are a multilobar, multipapillary form of mammalian kidneys, usually without signs of external lobulation. They are located on the left and rig ...
s or
liver The liver is a major metabolic organ (anatomy), organ exclusively found in vertebrates, which performs many essential biological Function (biology), functions such as detoxification of the organism, and the Protein biosynthesis, synthesis of var ...
; in small children, margosa oil is toxic and can lead to death. Margosa may also cause
miscarriage Miscarriage, also known in medical terms as a spontaneous abortion, is an end to pregnancy resulting in the loss and expulsion of an embryo or fetus from the womb before it can fetal viability, survive independently. Miscarriage before 6 weeks ...
s,
infertility In biology, infertility is the inability of a male and female organism to Sexual reproduction, reproduce. It is usually not the natural state of a healthy organism that has reached sexual maturity, so children who have not undergone puberty, whi ...
, and low blood sugar. In Southern India and the Middle-East, neem twigs are often used as a teeth-cleaning twig.


Pest and disease control

Margosa is a key ingredient in non-pesticidal management (NPM), providing a natural alternative to synthetic pesticides. Margosa seeds are ground into powder that is soaked overnight in water and sprayed on the crop. To be effective, it must be applied repeatedly, at least every ten days. Margosa does not directly kill
insect Insects (from Latin ') are Hexapoda, hexapod invertebrates of the class (biology), class Insecta. They are the largest group within the arthropod phylum. Insects have a chitinous exoskeleton, a three-part body (Insect morphology#Head, head, ...
s. It acts as an anti-feedant, repellent, and egg-laying deterrent and thus protects the crop from damage. The insects starve and die within a few days. Margosa also suppresses the subsequent hatching of their eggs. Margosa-based fertilizers have been effective against southern armyworm. Margosa cake may be used as a fertilizer. Margosa oil has been shown to avert
termite Termites are a group of detritivore, detritophagous Eusociality, eusocial cockroaches which consume a variety of Detritus, decaying plant material, generally in the form of wood, Plant litter, leaf litter, and Humus, soil humus. They are dist ...
attack as an ecofriendly and economical agent.


Other uses

* Tree: the margosa tree is of great importance for its anti-desertification properties and possibly as a good carbon dioxide sink. It is also used for maintaining soil fertility.Puhan, Sukumar, et al. "Mahua (Madhuca indica) seed oil: a source of renewable energy in India." (2005). *Fertilizer: neem extract is added to fertilizers (
urea Urea, also called carbamide (because it is a diamide of carbonic acid), is an organic compound with chemical formula . This amide has two Amine, amino groups (–) joined by a carbonyl functional group (–C(=O)–). It is thus the simplest am ...
) as a nitrification inhibitor. *Animal feed: margosa leaves can be occasionally used as forage for ruminants and rabbits. *Teeth cleaning: margosa has traditionally been used as a type of teeth-cleaning twig.


Toxicity

According to the American Journal of Neuroradiology, margosa oil has the ability to cause some forms of toxic encephalopathy and ophthalmopathy if consumed in quantities exceeding 150ml (5.07 us fluid ounces).


Genome and transcriptomes

Margosa genome and transcriptomes from various organs have been sequenced.
Expressed sequence tag In genetics, an expressed sequence tag (EST) is a short sub-sequence of a cDNA sequence. ESTs may be used to identify gene transcripts, and were instrumental in gene discovery and in gene-sequence determination. The identification of ESTs has pro ...
s were identified by generation of subtractive hybridization libraries of margosa fruit, leaf, fruit mesocarp, and fruit endocarp.


Cultural and social impact

The name Nimai ('born under a neem tree'), of the
Bhakti movement The Bhakti movement was a significant religious movement in medieval Hinduism that sought to bring religious reforms to all strata of society by adopting the method of Bhakti, devotion to achieve salvation. Originating in Tamilakam during 6t ...
Vaishnava saint and
Chaitanya Mahaprabhu Chaitanya Mahaprabhu (; ), born Vishvambhara Mishra () (18 February 1486 – 14 June 1534), was an Indian Hindus, Hindu saint from Bengal and the founder of Gaudiya Vaishnavism. Chaitanya Mahaprabhu's mode of worshipping Krishna with bha ...
(believed to be an incarnation of
Radha Krishna Radha-Krishna (IAST , ) is the combined form of the Hindu god Krishna with his chief consort and ''shakti'' Radha. They are regarded as the feminine as well as the masculine realities of God and gender in Hinduism, God, in several Krishnaism, Kr ...
in
Gaudiya Vaishnavism Gaudiya Vaishnavism (), also known as Chaitanya Vaishnavism, is a Vaishnavism, Vaishnava Hindu denominations, Hindu religious movement inspired by Chaitanya Mahaprabhu (1486–1534) in India. "Gaudiya" refers to the Gaura or Gauḍa region o ...
and
ISKCON The International Society for Krishna Consciousness (ISKCON), commonly known as the Hare Krishna movement, is a religious organization that follows the Gaudiya Vaishnavism, Gaudiya Vaishnava tradition of Hinduism. It was founded on 13 July 1966 ...
) is due to his birth under a neem tree. In 1995, the
European Patent Office The European Patent Office (EPO) is one of the two organs of the European Patent Organisation (EPOrg), the other being the Administrative Council. The EPO acts as executive body for the organisation
(EPO) granted a patent on an anti-fungal product derived from margosa to the
United States Department of Agriculture The United States Department of Agriculture (USDA) is an executive department of the United States federal government that aims to meet the needs of commercial farming and livestock food production, promotes agricultural trade and producti ...
and W. R. Grace and Company. The Indian government challenged the patent when it was granted, claiming that the process for which the patent had been granted had been in use in India for more than 2,000 years. In 2000, the EPO ruled in India's favour, but W. R. Grace appealed, claiming that prior art about the product had never been published. On 8 March 2005, that appeal was lost and the EPO revoked the Neem patent.


Biotechnology

The biopesticide produced by extraction from the tree seeds contains limonoid triterpenes. Currently, the extraction process has disadvantages such as contamination with fungi and heterogeneity in the content of limonoids due to genetic, climatic, and geographical variations. To overcome these problems, production of limonoids from plant cell suspension and hairy root cultures in bioreactors has been studied, including the development of a two-stage bioreactor process that enhances growth and production of limonoids with cell suspension cultures of ''A. indica''.


Gallery

File:Neem (Azadirachta indica) in Hyderabad W IMG 7006.jpg, Flowers File:Unripe Neem fruits.jpg, Unripe fruit File:Neem-Tree.jpg, Neem tree in a banana farm in India File:Ineem farm in virudhunagar.jpg, Neem tree farm File:Neem Twig sellers.JPG, Twigs for sale File:Ineem Fruit Drying process for cold pressed extraction.jpg, Fruit drying for oil extraction File:Jagnath Ineem Toothstick in tamilnadu.jpg, Cleaning teeth by chewing stick File:Hareli Festival.jpg, Native of Chhattisgarh with Neem branches and leaves for Hareli Festival File:Azadirachta indica 0001.jpg, A tree in
Gambia The Gambia, officially the Republic of The Gambia, is a country in West Africa. Geographically, The Gambia is the List of African countries by area, smallest country in continental Africa; it is surrounded by Senegal on all sides except for ...
File:Neem tree.JPG, A tree in Bangladesh File:Neem (Azadirachta indica) in Hyderabad W IMG 6976.jpg, Leaves and flowers


See also

* '' Azadirachta excelsa'' * Azadirachtin *
Ayurveda Ayurveda (; ) is an alternative medicine system with historical roots in the Indian subcontinent. It is heavily practised throughout India and Nepal, where as much as 80% of the population report using ayurveda. The theory and practice of ayur ...
*
Arid Forest Research Institute Arid Forest Research Institute (ICFRE-AFRI) is a research institute situated in Jodhpur, Rajasthan, India. The institute conducts scientific research in forestry in order to provide technologies to increase the Permanent vegetative cover, vege ...
(AFRI) * Neem cake *
Neem oil Neem oil, also known as margosa oil, is a vegetable oil pressed from the fruits and seeds of the neem (''Azadirachta indica''), a tree which is indigenous to the Indian subcontinent and has been introduced to many other areas in the tropics. It ...
* Babool (brand) of toothpaste * Teeth cleaning twig (datun)


References


External links

*
PDF Copy


* ttp://www.hear.org/species/azadirachta_indica/ Neem informationfrom the Hawaiian Ecosystems at Risk project (HEAR) * Contains a detailed monograph on ''Azadirachta indica'' (Neem; Nimba) as well as a discussion of health benefits and usage in clinical practice. {{EngvarB, date=March 2021 indica Flora of Assam (region) Flora of Indo-China Flora of Bangladesh Trees of the Indian subcontinent Plants in Hinduism Drought-tolerant trees