Mikhail Tsvet
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Mikhail Semyonovich Tsvet (Михаил Семёнович Цвет, also spelled Tsvett, Tswett, Tswet, Zwet, and Cvet; 14 May 1872 – 26 June 1919) was a Russian-Italian botanist who invented
chromatography In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the ''mobile phase'', which carries it through a system ( ...
. His last name is Russian for "colour" and is also the root word of "
flower A flower, sometimes known as a bloom or blossom, is the reproductive structure found in flowering plants (plants of the division Angiospermae). The biological function of a flower is to facilitate reproduction, usually by providing a mechanism ...
."


Biography

Mikhail Tsvet was born 14 May 1872 in Asti, Italy. His mother was Italian, and his father was a Russian official. His mother died soon after his birth, and he was raised in
Geneva Geneva ( ; french: Genève ) frp, Genèva ; german: link=no, Genf ; it, Ginevra ; rm, Genevra is the second-most populous city in Switzerland (after Zürich) and the most populous city of Romandy, the French-speaking part of Switzerland. Situ ...
, Switzerland. He received his BS degree from the Department of Physics and Mathematics at the University of Geneva in 1893. However, he decided to dedicate himself to
botany Botany, also called plant science (or plant sciences), plant biology or phytology, is the science of plant life and a branch of biology. A botanist, plant scientist or phytologist is a scientist who specialises in this field. The term "bot ...
and received his PhD degree in 1896 for his work on cell
physiology Physiology (; ) is the scientific study of functions and mechanisms in a living system. As a sub-discipline of biology, physiology focuses on how organisms, organ systems, individual organs, cells, and biomolecules carry out the chemic ...
. He moved to
Saint Petersburg Saint Petersburg ( rus, links=no, Санкт-Петербург, a=Ru-Sankt Peterburg Leningrad Petrograd Piter.ogg, r=Sankt-Peterburg, p=ˈsankt pʲɪtʲɪrˈburk), formerly known as Petrograd (1914–1924) and later Leningrad (1924–1991), i ...
, Russia, in 1896 because his father was recalled from the foreign service. There he started to work at the Biological Laboratory of the
Russian Academy of Sciences The Russian Academy of Sciences (RAS; russian: Росси́йская акаде́мия нау́к (РАН) ''Rossíyskaya akadémiya naúk'') consists of the national academy of Russia; a network of scientific research institutes from across t ...
. His Geneva degrees were not recognized in Russia, and he had to earn Russian degrees. In 1897 he became a teacher of botany courses for women. In 1902 he became a laboratory assistant at the Institute of Plant Physiology of the
Warsaw University The University of Warsaw ( pl, Uniwersytet Warszawski, la, Universitas Varsoviensis) is a public university in Warsaw, Poland. Established in 1816, it is the largest institution of higher learning in the country offering 37 different fields of ...
(now in Poland). In 1903 he became an assistant professor and taught also at other Warsaw universities. After the beginning of
World War I World War I (28 July 1914 11 November 1918), often abbreviated as WWI, was List of wars and anthropogenic disasters by death toll, one of the deadliest global conflicts in history. Belligerents included much of Europe, the Russian Empire, ...
the
Warsaw University of Technology The Warsaw University of Technology ( pl, Politechnika Warszawska, lit=Varsovian Polytechnic) is one of the leading institutes of technology in Poland and one of the largest in Central Europe. It employs 2,453 teaching faculty, with 357 professor ...
was evacuated to Moscow, Russia, and in 1916 again to
Gorki Gorki may refer to: * Gorki Águila (b. 1968), Cuban rock musician * Gorki (band), a Belgian band of Luc De Vos * Gorki (Kazan Metro), a station of the Kazan Metro, Kazan, Russia * Gorki Ridge, a ridge in Antarctica * Gorki, Russia, several inh ...
near Moscow. In 1917 he became a Professor of Botany and the director of the
botanical garden A botanical garden or botanic gardenThe terms ''botanic'' and ''botanical'' and ''garden'' or ''gardens'' are used more-or-less interchangeably, although the word ''botanic'' is generally reserved for the earlier, more traditional gardens, an ...
s at the
University of Tartu The University of Tartu (UT; et, Tartu Ülikool; la, Universitas Tartuensis) is a university in the city of Tartu in Estonia. It is the national university of Estonia. It is the only classical university in the country, and also its biggest ...
(Yuryev) (now in
Estonia Estonia, formally the Republic of Estonia, is a country by the Baltic Sea in Northern Europe. It is bordered to the north by the Gulf of Finland across from Finland, to the west by the sea across from Sweden, to the south by Latvia, and t ...
). In 1918 when German troops occupied the city, the university was evacuated to
Voronezh Voronezh ( rus, links=no, Воро́неж, p=vɐˈronʲɪʂ}) is a city and the administrative centre of Voronezh Oblast in southwestern Russia straddling the Voronezh River, located from where it flows into the Don River. The city sits on ...
, a large city in the south of
Central Russia Central Russia is, broadly, the various areas in European Russia. Historically, the area of Central Russia varied based on the purpose for which it is being used. It may, for example, refer to European Russia (except the North Caucasus and ...
. Tsvet died of a chronic inflammation of the throat on 26 June 1919 at the age of 47.


Chromatography

Mikhail Tsvet invented chromatography in 1900 during his research on plant
pigment A pigment is a colored material that is completely or nearly insoluble in water. In contrast, dyes are typically soluble, at least at some stage in their use. Generally dyes are often organic compounds whereas pigments are often inorganic comp ...
s. He used liquid-adsorption
column chromatography Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential adsorption of compounds to the adsorbent; compounds move t ...
with calcium carbonate as
adsorbent Adsorption is the adhesion of atoms, ions or molecules from a gas, liquid or dissolved solid to a surface. This process creates a film of the ''adsorbate'' on the surface of the ''adsorbent''. This process differs from absorption, in which ...
and petrol ether/
ethanol Ethanol (abbr. EtOH; also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound. It is an alcohol with the chemical formula . Its formula can be also written as or (an ethyl group linked to a h ...
mixtures as
eluent In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent; as in washing of loaded ion-exchange resins to remove captured ions. In a liquid chromatography experiment, for exa ...
to separate
chlorophyll Chlorophyll (also chlorophyl) is any of several related green pigments found in cyanobacteria and in the chloroplasts of algae and plants. Its name is derived from the Greek words , ("pale green") and , ("leaf"). Chlorophyll allow plants to ...
s and
carotenoid Carotenoids (), also called tetraterpenoids, are yellow, orange, and red organic pigments that are produced by plants and algae, as well as several bacteria, and fungi. Carotenoids give the characteristic color to pumpkins, carrots, parsnips, cor ...
s. The method was described on 30 December 1901 at the XI Congress of Naturalists and Physicians (XI съезд естествоиспытателей и врачей) in St. Petersburg. The first printed description was in 1905, in the ''Proceedings of the Warsaw Society of Naturalists, biology section''. He first used the term "chromatography" in print in 1906 in his two papers about chlorophyll in the German botanical journal, ''Berichte der Deutschen botanischen Gesellschaft''. In 1907 he demonstrated his chromatograph for the German Botanical Society. For several reasons, Tsvet's work was long ignored: the violent political upheaval in Russia at the beginning of the 20th century, the fact that Tsvet originally published only in Russian (making his results largely inaccessible to western scientists), and an article denying Tsvet's findings. Richard Willstätter and Arthur Stoll tried to repeat Tsvet's experiments, but because they used an overly aggressive adsorbent (destroying the chlorophyll), were not able to do so. They published their results and Tsvet's chromatography method fell into obscurity. It was revived 10 years after his death thanks to Austrian
biochemist Biochemists are scientists who are trained in biochemistry. They study chemical processes and chemical transformations in living organisms. Biochemists study DNA, proteins and cell parts. The word "biochemist" is a portmanteau of "biological che ...
Richard Kuhn and his student, German scientist
Edgar Lederer Edgar is a commonly used English given name, from an Anglo-Saxon name ''Eadgar'' (composed of '' ead'' "rich, prosperous" and ''gar'' "spear"). Like most Anglo-Saxon names, it fell out of use by the later medieval period; it was, however, rev ...
as well as the work of
A. J. Martin A is the first letter of the Latin and English alphabet. A may also refer to: Science and technology Quantities and units * ''a'', a measure for the attraction between particles in the Van der Waals equation * ''A'' value, a measure o ...
and R. L. Synge.


Botanical author abbreviation

The standard
botanical author abbreviation In botanical nomenclature, author citation is the way of citing the person or group of people who validly published a botanical name, i.e. who first published the name while fulfilling the formal requirements as specified by the ''International Cod ...
Tswett is applied to plants that he described.


References


External links

*
R. Willstätter, A. Stoll, Untersuchungen über Chlorophyll
Springer, Berlin (1913)
Biography of Mikhail S. Tsvet
(pdf, in German)

Berichte der Deutschen botanischen Gesellschaft 24, 316–323 (1906) {{DEFAULTSORT:Tsvet, Mikhail 1872 births 1919 deaths 19th-century botanists from the Russian Empire 20th-century Russian botanists People from the Russian Empire of Italian descent University of Tartu faculty Inventors from the Russian Empire Botanists with author abbreviations Italian emigrants to the Russian Empire Chromatography