MMN medium or Modified Melin-Norkrans medium is a type of

agar Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from " ogonori" and " tengusa". As found in nature, agar is a mixture of two components, t ...

growth medium, used to grow cultures of

mycorrhiza A mycorrhiza (; , mycorrhiza, or mycorrhizas) is a symbiotic association between a fungus and a plant. The term mycorrhiza refers to the role of the fungus in the plant's rhizosphere, the plant root system and its surroundings. Mycorrhizae play ...

fungi A fungus (: fungi , , , or ; or funguses) is any member of the group of eukaryotic organisms that includes microorganisms such as yeasts and mold (fungus), molds, as well as the more familiar mushrooms. These organisms are classified as one ...

, such as ''

Boletus edulis ''Boletus edulis'' (English: cep, penny bun, porcino) is a basidiomycete fungus, and the type species of the genus ''Boletus''. It is prized as an edible mushroom. The fungus produces Basidiospore, spore-bearing basidiocarp, fruit bodies ...

'' and ''

Tricholoma matsutake , ''Tricholoma matsutake'', is a species of choice edible mycorrhizal mushroom that grows in Eurasia and North America. It is prized in Japanese cuisine for its distinct odor. Etymology The common name and specific epithet, ''matsutake'', i ...

''. It was first described by DH. Marx in ''The influence of ectotrophic mycorrhizal fungi on the resistance of pine roots to pathogenic infections. I. Antagonism of mycorrhizal fungi to root pathogenic fungi and soil bacteria'' in 1969. The acidic pH (5.6) of MMN agar inhibits bacterial growth.

Typical composition

MMN agar typically contains: * 10 g/L

glucose Glucose is a sugar with the Chemical formula#Molecular formula, molecular formula , which is often abbreviated as Glc. It is overall the most abundant monosaccharide, a subcategory of carbohydrates. It is mainly made by plants and most algae d ...

* 3 g/L

malt extract Malt is any cereal grain that has been made to germinate by soaking in water and then stopped from germinating further by drying with hot air, a process known as " malting". Malted grain is used to make beer, whisky, malted milk, malt vinegar ...

* 0.25 g/L (NH₄)₂HPO₄ * 0.025 g/L

NaCl Sodium chloride , commonly known as edible salt, is an ionic compound with the chemical formula NaCl, representing a 1:1 ratio of sodium and chloride ions. It is transparent or translucent, brittle, hygroscopic, and occurs as the mineral hali ...

* 0.5 g/L KH₂PO₄ * 0.05 g/L CaCl₂ * 0.15 g/L MgSO₄.7 H₂O * 0.012g/L FeCl₃. 6 H₂O * 0.003 g/L

thiamine Thiamine, also known as thiamin and vitamin B1, is a vitamin – an Nutrient#Micronutrients, essential micronutrient for humans and animals. It is found in food and commercially synthesized to be a dietary supplement or medication. Phosp ...

* 15 g/L

References

Microbiological media {{Microbiology-stub