Light green SF, also called C.I. 42095, light green SF yellowish, is a green

triarylmethane dye Triarylmethane dyes are synthetic organic compounds containing triphenylmethane backbones. As dyes, these compounds are intensely colored. They are produced industrially as dyes. Families Triarylmethane dyes can be grouped into families accordin ...

Uses

Biomedical

It is used in

histology Histology, also known as microscopic anatomy or microanatomy, is the branch of biology which studies the microscopic anatomy of biological tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures vi ...

for

staining Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues), in cytology (microscopic study of cells), and in ...

collagen; for that purpose it is a standard dye in North America. In Masson's trichrome it is used as a

counterstain A counterstain is a stain with colour contrasting to the principal stain, making the stained structure easily visible using a microscope. Examples include the malachite green counterstain to the fuchsine stain in the Gimenez staining techniqu ...

to acid

fuchsin Fuchsine (sometimes spelled fuchsin) or rosaniline hydrochloride is a magenta dye with chemical formula C20H19N3·HCl.

. It is a component of

Papanicolaou stain Papanicolaou stain (also Papanicolaou's stain and Pap stain) is a multichromatic (multicolored) cytological staining technique developed by George Papanicolaou in 1942. The Papanicolaou stain is one of the most widely used stains in cytology, whe ...

s together with

eosin Y Eosin Y, also called C.I. 45380 or C.I. Acid Red 87, is a member of the triarylmethane dyes. It is produced from fluorescein by bromination. Use Eosin Y is commonly used as the red dye in red inks. It is commonly used in histology, most notab ...

and bismarck brown Y. In pap smears, Light Green SF confers a blue staining for the

cytoplasm In cell biology, the cytoplasm is all of the material within a eukaryotic cell, enclosed by the cell membrane, except for the cell nucleus. The material inside the nucleus and contained within the nuclear membrane is termed the nucleoplasm. ...

of active cells such as columnar cells, parabasal squamous cells, and intermediate squamous cells. It usually comes as a disodium salt. Its maximum absorption is at 630 (422) nm. The dye is not very durable — it has a tendency to fade. When fading is to be avoided, it is replaced with

fast green FCF Fast Green FCF, also called Food green 3, FD&C Green No. 3, Green 1724, Solid Green FCF, and C.I. 42053, is a turquoise triarylmethane food dye. Its E number is E143. Fast Green FCF is recommended as a replacement of Light Green SF yellowish in ...

, which also has more brilliant color. Fast green FCF can also substitute light green SF in other procedures. Lissamine green dye can be used to check the health of the anterior surfaces of the eye. It is available on a swab, which is wet with saline and then the dye is dropped into the lower fornix. The dye shows up conjunctival staining similar to rose Bengal dye but it does not sting like rose Bengal does.

Food coloring

Light green SF was once used as a green food colorant. Its use in the U.S. was discontinued due to its low popularity.

References

{{DEFAULTSORT:Light Green Sf Yellowish Triarylmethane dyes Staining dyes Benzenesulfonates Anilines