Interstate 335 may refer to:
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Interstate 335 (Iowa), a designation once proposed by city leaders in Carlisle and Des Moines, Iowa for the Des Moines Bypass
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Interstate 335 (Kansas), a spur to Topeka, Kansas and designation for part of the Kansas Turnpike
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Interstate 335 (Minnesota), a never-built spur in Minneapolis, Minnesota
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Interstate 335 (Oklahoma), the designation for the Kickapoo Turnpike
{{road disambiguation
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