Enzalutamide, sold under the brand name Xtandi, is a
nonsteroidal antiandrogen (NSAA) medication which is used in the treatment of
prostate cancer
Prostate cancer is cancer of the prostate. Prostate cancer is the second most common cancerous tumor worldwide and is the fifth leading cause of cancer-related mortality among men. The prostate is a gland in the male reproductive system that su ...
.
It is indicated for use in conjunction with
castration in the treatment of
metastatic castration-resistant prostate cancer (mCRPC),
nonmetastatic castration-resistant prostate cancer,
and metastatic castration-sensitive prostate cancer (mCSPC). It is taken
by mouth
Oral administration is a route of administration where a substance is taken through the mouth. Per os abbreviated to P.O. is sometimes used as a direction for medication to be taken orally. Many medications are taken orally because they are i ...
.
Side effects of enzalutamide when added to castration include
asthenia,
back pain,
diarrhea
Diarrhea, also spelled diarrhoea, is the condition of having at least three loose, liquid, or watery bowel movements each day. It often lasts for a few days and can result in dehydration due to fluid loss. Signs of dehydration often begin ...
,
arthralgia, and
hot flashes.
Rarely, it can cause
seizures.
It has a high potential for
drug interactions.
Enzalutamide is an
antiandrogen, and acts as an
antagonist of the
androgen receptor, the
biological target of
androgens like
testosterone and
dihydrotestosterone.
In doing so, it prevents the effects of these
hormones in the
prostate gland and elsewhere in the body.
Enzalutamide was first described in 2006, and was introduced for the treatment of prostate cancer in 2012.
It was the first
second-generation NSAA
A nonsteroidal antiandrogen (NSAA) is an antiandrogen with a nonsteroidal chemical structure. They are typically selective and full or silent antagonists of the androgen receptor (AR) and act by directly blocking the effects of androgens lik ...
to be introduced.
It is on the
World Health Organization's List of Essential Medicines.
Medical uses
Prostate cancer
There is good evidence that enzalutamide is an effective treatment for increasing overall survival among people with high-risk non-metastatic castration-resistant prostate cancer, particularly those with a
PSA doubling time ≤ 6 months.
Other uses
Enzalutamide can be used as an antiandrogen in
feminizing hormone therapy for
transgender women.
Available forms
Enzalutamide is provided in the form of a 40 mg Capsule.
It is taken orally at a dosage of 160 mg once per day (four capsules).
Contraindications
Enzalutamide is
contraindicated in women during
pregnancy.
It may cause
fetal harm.
Side effects
Notable
side effects of enzalutamide seen in
clinical trial
Clinical trials are prospective biomedical or behavioral research studies on human subject research, human participants designed to answer specific questions about biomedical or behavioral interventions, including new treatments (such as novel v ...
s have included
gynecomastia,
breast pain/tenderness,
fatigue,
diarrhea
Diarrhea, also spelled diarrhoea, is the condition of having at least three loose, liquid, or watery bowel movements each day. It often lasts for a few days and can result in dehydration due to fluid loss. Signs of dehydration often begin ...
,
hot flashes,
headache,
sexual dysfunction, and, less commonly,
seizures.
Other "common" side effects reported in clinical trials have included
neutropenia,
visual hallucinations,
anxiety
Anxiety is an emotion which is characterized by an unpleasant state of inner turmoil
Turmoil may refer to:
* ''Turmoil'' (1984 video game), a 1984 video game released by Bug-Byte
* ''Turmoil'' (2016 video game), a 2016 indie oil tycoon video ...
,
cognitive disorder,
memory impairment,
hypertension,
dry skin, and
pruritus (itching).
Enzalutamide monotherapy is regarded as having a moderate negative effect on sexual function and activity, significantly less than that of GnRH analogues but similar to that of other NSAAs such as bicalutamide.
Central adverse effects
Seizures have occurred in approximately 1% of patients treated with enzalutamide in clinical trials.
This is thought to be due to enzalutamide crossing the
blood–brain barrier and exerting off-target binding to and inhibition of the
GABAA receptor in the
central nervous system
The central nervous system (CNS) is the part of the nervous system consisting primarily of the brain and spinal cord. The CNS is so named because the brain integrates the received information and coordinates and influences the activity of all p ...
(it has been found to inhibit the GABA
A receptor ''
in vitro
''In vitro'' (meaning in glass, or ''in the glass'') studies are performed with microorganisms, cells, or biological molecules outside their normal biological context. Colloquially called "test-tube experiments", these studies in biology and ...
'' ( = 3.6 μM)
and induces
convulsion
A convulsion is a medical condition where the body muscles contract and relax rapidly and repeatedly, resulting in uncontrolled shaking. Because epileptic seizures typically include convulsions, the term ''convulsion'' is sometimes used as a ...
s in animals at high doses).
In addition to seizures, other potentially GABA
A receptor-related side effects observed with enzalutamide treatment in clinical trials have included
anxiety
Anxiety is an emotion which is characterized by an unpleasant state of inner turmoil
Turmoil may refer to:
* ''Turmoil'' (1984 video game), a 1984 video game released by Bug-Byte
* ''Turmoil'' (2016 video game), a 2016 indie oil tycoon video ...
,
insomnia,
vertigo
Vertigo is a condition where a person has the sensation of movement or of surrounding objects moving when they are not. Often it feels like a spinning or swaying movement. This may be associated with nausea, vomiting, sweating, or difficulties ...
,
paresthesia, and headache.
Due to its ability to lower the
seizure threshold, patients with known
seizure disorders or
brain injury should be closely monitored during enzalutamide treatment.
NSAA-induced seizures are responsive to
benzodiazepine treatment, and it has been suggested that GABA
A receptor inhibition by enzalutamide could be treated with these drugs.
In
dose-ranging studies, severe
fatigue was observed with enzalutamide at doses of 240 mg/day and above.
Rare adverse reactions
There is a single
case report In medicine, a case report is a detailed report of the symptoms, signs, diagnosis, treatment, and follow-up of an individual patient. Case reports may contain a demographic profile of the patient, but usually describe an unusual or novel occurr ...
of
posterior reversible encephalopathy syndrome (PRES) with enzalutamide treatment.
The
mechanism of action of the side effect is unknown, but it was proposed to a consequence of inhibition of the GABA
A receptor by enzalutamide.
Overdose
Enzalutamide may cause
seizures in
overdose.
Interactions
Enzalutamide is a moderate to strong inducer of multiple
cytochrome P450
Cytochromes P450 (CYPs) are a superfamily of enzymes containing heme as a cofactor that functions as monooxygenases. In mammals, these proteins oxidize steroids, fatty acids, and xenobiotics, and are important for the clearance of various compo ...
enzyme
Enzymes () are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrate (chemistry), substrates, and the enzyme converts the substrates into different molecule ...
s including
CYP3A4,
CYP2C9, and
CYP2C19 and hence has a high potential for clinically relevant
drug interactions.
Circulating concentrations of enzalutamide may be altered by
inhibitor
Inhibitor or inhibition may refer to:
In biology
* Enzyme inhibitor, a substance that binds to an enzyme and decreases the enzyme's activity
* Reuptake inhibitor, a substance that increases neurotransmission by blocking the reuptake of a neurotra ...
s and
inducers of
CYP2C8 and CYP3A4, and should be avoided if possible.
In a clinical study of enzalutamide for -positive
breast cancer
Breast cancer is cancer that develops from breast tissue. Signs of breast cancer may include a lump in the breast, a change in breast shape, dimpling of the skin, milk rejection, fluid coming from the nipple, a newly inverted nipple, or ...
in women, enzalutamide was found to decrease serum concentrations of the
aromatase inhibitors
anastrozole and
exemestane by 90% and 50%, respectively, which could reduce their effectiveness.
Pharmacology
Pharmacodynamics
Enzalutamide acts as a
selective silent antagonist of the
androgen receptor (AR), the
biological target of
androgens like
testosterone and
dihydrotestosterone (DHT). Unlike the first-generation NSAA
bicalutamide, enzalutamide does not promote
translocation
Translocation may refer to:
* Chromosomal translocation, a chromosome abnormality caused by rearrangement of parts
** Robertsonian translocation, a chromosomal rearrangement in pairs 13, 14, 15, 21, and 22
** Nonreciprocal translocation, transfer ...
of AR to the
cell nucleus and in addition prevents binding of AR to
deoxyribonucleic acid (DNA) and AR to
coactivator protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, respon ...
s.
As such, it has been described as an AR signaling inhibitor in addition to antagonist.
The drug is described as a "second-generation" NSAA because it has greatly increased efficacy as an antiandrogen relative to so-called "first-generation" NSAAs like
flutamide and bicalutamide. The drug has only 2-fold lower affinity for the AR than DHT, the endogenous ligand of the AR in the prostate gland.
When
LNCaP cells (a prostate cancer cell line) engineered to express elevated levels of AR (as found in patients with advanced prostate cancer) were treated with enzalutamide, the expression of androgen-dependent genes PSA and
TMPRSS2 was down regulated in contrast to bicalutamide where the expression was upregulated.
In VCaP cells which over-express the AR, enzalutamide induced
apoptosis whereas bicalutamide did not.
Furthermore, enzalutamide behaves as an antagonist of the W741C mutant AR in contrast to bicalutamide which behaves as a pure
agonist when bound to the W741C mutant.
Dose-ranging studies of enzalutamide in men with prostate cancer have been performed.
Changes in hormone levels
Enzalutamide monotherapy at a dosage of 160 mg/day has been found to increase circulating levels of
testosterone by 114.3%,
dihydrotestosterone (DHT) by 51.7%,
estradiol by 71.7%,
sex hormone-binding globulin (SHBG) by 100.6%,
dehydroepiandrosterone (DHEA) by 9.6%,
androstenedione
Androstenedione, or 4-androstenedione (abbreviated as A4 or Δ4-dione), also known as androst-4-ene-3,17-dione, is an endogenous weak androgen steroid hormone and intermediate in the biosynthesis of estrone and of testosterone from dehy ...
by 51.1%,
luteinizing hormone (LH) by 184.7%,
follicle-stimulating hormone (FSH) by 47.0%, and
prolactin by 16.8%.
These changes in hormone levels are similar to those with high-dose bicalutamide monotherapy.
The median maximum decrease in levels of
prostate-specific antigen (PSA) levels was 99.6%.
Comparison with other antiandrogens
Enzalutamide has approximately 8-fold higher
binding affinity for the
androgen receptor (AR) compared to
bicalutamide.
One study found an of 21 nM for enzalutamide and 160 nM for bicalutamide at the AR in the
LNCaP cell line (7.6-fold difference),
while another found respective IC
50 values of 36 nM and 159 nM (4.4-fold difference).
In accordance, clinical findings suggest that enzalutamide is a significantly more potent and effective antiandrogen in comparison to first-generation NSAAs such as bicalutamide, flutamide, and nilutamide.
Also, unlike with the first-generation NSAAs, there has been no evidence of
hepatotoxicity
Hepatotoxicity (from ''hepatic toxicity'') implies chemical-driven liver damage. Drug-induced liver injury is a cause of acute and chronic liver disease caused specifically by medications and the most common reason for a drug to be withdrawn fr ...
or
elevated liver enzymes in association with enzalutamide treatment in clinical trials.
Resistance mechanisms in prostate cancer
Enzalutamide is only effective for a certain period of time, after that the growth of the cancer is not inhibited by this antiandrogen. The mechanisms of resistance to Enzalutamide are being intensively studied.
Currently, several mechanisms have been found:
* AR mutations
* AR
splice variants
*
Glucocorticoid receptor bypass
* Increase in
flux
Flux describes any effect that appears to pass or travel (whether it actually moves or not) through a surface or substance. Flux is a concept in applied mathematics and vector calculus which has many applications to physics. For transport ...
of
glycolysis
*
Autophagy mediated resistance
*
Wnt signaling activation
* Increase in intra-tumoral androgen biosynthesis mediated by
AKR1C3
Aldo-keto reductase family 1 member C3 (AKR1C3), also known as 17β-hydroxysteroid dehydrogenase type 5 (17β-HSD5, HSD17B5) is a key steroidogenic enzyme that in humans is encoded by the ''AKR1C3'' gene.
Function
This gene encodes a member of t ...
enzyme
*
Interleukin 6 signaling mediated resistance
Cytochrome P450 modulation
Enzalutamide is reported to be a strong
inducer of the
enzyme
Enzymes () are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrate (chemistry), substrates, and the enzyme converts the substrates into different molecule ...
CYP3A4 and a moderate inducer of
CYP2C9 and
CYP2C19, and can affect the circulating concentrations of drugs that are metabolized by these enzymes.
Pharmacokinetics
The
bioavailability of enzalutamide in humans is unknown, but is at least 84.6% based on the amount recovered from
urine
Urine is a liquid by-product of metabolism in humans and in many other animals. Urine flows from the kidneys through the ureters to the urinary bladder. Urination results in urine being excreted from the body through the urethra.
Cellular ...
and
bile
Bile (from Latin ''bilis''), or gall, is a dark-green-to-yellowish-brown fluid produced by the liver of most vertebrates that aids the digestion of lipids in the small intestine. In humans, bile is produced continuously by the liver (liver b ...
in
excretion
Excretion is a process in which metabolic waste
is eliminated from an organism. In vertebrates this is primarily carried out by the lungs, kidneys, and skin. This is in contrast with secretion, where the substance may have specific tasks afte ...
studies.
Similarly, the bioavailability of enzalutamide in rats is 89.7%.
Steady-state concentrations of enzalutamide are achieved within 28 days of treatment initiation.
The
plasma protein binding of enzalutamide is 97 to 98%, while that of
''N''-desmethylenzalutamide (NDME), its major
metabolite, is 95%.
Enzalutamide is primarily bound to
albumin.
The medication is
metabolized in the
liver
The liver is a major organ only found in vertebrates which performs many essential biological functions such as detoxification of the organism, and the synthesis of proteins and biochemicals necessary for digestion and growth. In humans, it ...
, mainly by the
cytochrome P450
Cytochromes P450 (CYPs) are a superfamily of enzymes containing heme as a cofactor that functions as monooxygenases. In mammals, these proteins oxidize steroids, fatty acids, and xenobiotics, and are important for the clearance of various compo ...
enzyme
Enzymes () are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrate (chemistry), substrates, and the enzyme converts the substrates into different molecule ...
s
CYP2C8 and
CYP3A4.
CYP2C8 is primarily responsible for the formation of NDME.
Enzalutamide has a long
elimination half-life of 5.8 days on average, with a range of 2.8 to 10.2 days.
The elimination half-life of NDME is even longer, at about 7.8 to 8.6 days.
Enzalutamide is
eliminated 71.0% in
urine
Urine is a liquid by-product of metabolism in humans and in many other animals. Urine flows from the kidneys through the ureters to the urinary bladder. Urination results in urine being excreted from the body through the urethra.
Cellular ...
, 13.6% in
bile
Bile (from Latin ''bilis''), or gall, is a dark-green-to-yellowish-brown fluid produced by the liver of most vertebrates that aids the digestion of lipids in the small intestine. In humans, bile is produced continuously by the liver (liver b ...
, and 0.39% in
feces
Feces ( or faeces), known colloquially and in slang as poo and poop, are the solid or semi-solid remains of food that was not digested in the small intestine, and has been broken down by bacteria in the large intestine. Feces contain a rela ...
.
Chemistry
Enzalutamide is a
synthetic Synthetic things are composed of multiple parts, often with the implication that they are artificial. In particular, 'synthetic' may refer to:
Science
* Synthetic chemical or compound, produced by the process of chemical synthesis
* Synthetic o ...
di
aryl thiohydantoin derivative
In mathematics, the derivative of a function of a real variable measures the sensitivity to change of the function value (output value) with respect to a change in its argument (input value). Derivatives are a fundamental tool of calculus. ...
and is structurally related to the earlier first-generation NSAAs such as
flutamide,
nilutamide, and
bicalutamide as well as to newer second-generation NSAAs like
apalutamide and
proxalutamide.
History
Enzalutamide was discovered by
Charles Sawyers, now at the
Memorial Sloan–Kettering Cancer Center, and
Michael Jung at the
University of California, Los Angeles
The University of California, Los Angeles (UCLA) is a public university, public Land-grant university, land-grant research university in Los Angeles, California. UCLA's academic roots were established in 1881 as a Normal school, teachers colle ...
.
They and their colleagues
synthesized and evaluated nearly 200
thiohydantoin derivative
In mathematics, the derivative of a function of a real variable measures the sensitivity to change of the function value (output value) with respect to a change in its argument (input value). Derivatives are a fundamental tool of calculus. ...
s of
RU-59063, an
analogue of
nilutamide, for AR antagonism in human prostate cancer cells, and identified enzalutamide and
RD-162 as lead compounds.
These compounds were
patent
A patent is a type of intellectual property that gives its owner the legal right to exclude others from making, using, or selling an invention for a limited period of time in exchange for publishing an sufficiency of disclosure, enabling disclo ...
ed in 2006 and described in 2007.
[Sawyers, C., Jung, M., Chen, C., Ouk, S., Welsbie, D., Tran, C., ... & Yoo, D. (2006). U.S. Patent Application No. 11/433,829. https://www.google.com/patents/US20070004753] Enzalutamide was developed and marketed by
Medivation
Medivation was an American biopharmaceutical company focused on development of novel therapies to treat serious diseases for which there are limited treatment options. Medivation was headquartered in San Francisco, California, beginning operations ...
for the treatment of prostate cancer.
It was approved by the U.S.
Food and Drug Administration
The United States Food and Drug Administration (FDA or US FDA) is a federal agency of the Department of Health and Human Services. The FDA is responsible for protecting and promoting public health through the control and supervision of food ...
(FDA) for the treatment of mCRPC in the United States in August 2012, and for the treatment of nonmetastatic castration-resistant prostate cancer in July 2018.
Enzalutamide was the first new AR antagonist to be approved for the treatment of prostate cancer in over 15 years, following the introduction of the first-generation NSAA
bicalutamide in 1995.
It was the first second-generation NSAA to be introduced.
Research
Breast cancer
Research suggests that enzalutamide may be effective in the treatment of certain types of
breast cancer
Breast cancer is cancer that develops from breast tissue. Signs of breast cancer may include a lump in the breast, a change in breast shape, dimpling of the skin, milk rejection, fluid coming from the nipple, a newly inverted nipple, or ...
in women.
It has been tested for the treatment of triple-negative, AR-positive breast cancer in a
phase II Phase II, Phase 2 or Phase Two may refer to:
Media
* Marvel Cinematic Universe: Phase Two, six American superhero films from 2013–2015
* ''Star Trek: Phase II'', an unrealized television series based on the characters of Gene Roddenberry's ''S ...
clinical trial
Clinical trials are prospective biomedical or behavioral research studies on human subject research, human participants designed to answer specific questions about biomedical or behavioral interventions, including new treatments (such as novel v ...
.
Hirsutism
Enzalutamide has been suggested as a potential treatment for
hirsutism and
hyperandrogenism in women with
polycystic ovary syndrome.
References
External links
*
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