In molecular biology ctRNA (counter-transcribed

RNA Ribonucleic acid (RNA) is a polymeric molecule that is essential for most biological functions, either by performing the function itself (non-coding RNA) or by forming a template for the production of proteins (messenger RNA). RNA and deoxyrib ...

) is a plasmid encoded

noncoding RNA A non-coding RNA (ncRNA) is a functional RNA molecule that is not translated into a protein. The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene. Abundant and functionally important types of non-co ...

that binds to the

mRNA In molecular biology, messenger ribonucleic acid (mRNA) is a single-stranded molecule of RNA that corresponds to the genetic sequence of a gene, and is read by a ribosome in the process of Protein biosynthesis, synthesizing a protein. mRNA is ...

of and causes translational inhibition. ctRNA is encoded by plasmids and functions in

rolling circle replication Rolling circle replication (RCR) is a process of unidirectional nucleic acid replication that can rapidly synthesize multiple copies of circular molecules of DNA or RNA, such as plasmids, the genomes of bacteriophages, and the circular RNA genom ...

to maintain a low copy number. In ''

Corynebacterium glutamicum ''Corynebacterium glutamicum'' is a Gram-positive, rod-shaped bacterium that is used industrially for large-scale production of amino acids, especially glutamic acid and lysine. While originally identified in a screen for organisms secreting L- ...

'', it achieves this by

antisense In molecular biology and genetics, the sense of a nucleic acid molecule, particularly of a strand of DNA or RNA, refers to the nature of the roles of the strand and its complement in specifying a sequence of amino acids. Depending on the context, ...

pairing with the mRNA of , a replication initiation protein. In ''

Enterococcus faecium ''Enterococcus faecium'' is a Gram-positive, gamma-hemolytic or non-hemolytic bacterium in the genus ''Enterococcus''. It can be commensal (innocuous, coexisting organism) in the gastrointestinal tract of humans and animals, but it may also be ...

'' the plasmid pJB01 contains three open reading frames, copA, , and . The pJB01 ctRNA is coded on the opposite strand from the / intergenic region and partially overlaps an atypical ribosome binding site for .

References

External links

* * * * Antisense RNA Genetics techniques {{molecular-cell-biology-stub

See also

References

External links