Campylobacter

''Campylobacter'' is a type of bacteria that can cause a diarrheal disease in people. Its name means "curved bacteria", as the germ typically appears in a comma or "s" shape. According to its scientific classification, it is a genus of gram-negative bacteria that is motile.

The germ is common in nature and in domestic animals. It is frequently found in raw food of vegetable and animal origin. Its numbers can be very high in some foods, like raw poultry. Due to their diverse natural reservoir, some ''Campylobacter'' can also be detected in the air, although not at an epidemiologically significant level. The disease that some of the species of the bacteria can cause is called campylobacteriosis.

At least a dozen species of ''Campylobacter'' have been implicated in human disease, with ''C. jejuni'' (80–90%) and '' C. coli'' (5–10%) being the most common. '' C. jejuni'' is recognized as one of the main causes of bacterial foodborne disease in many developed countries. It is the number one cause of bacterial gastroenteritis in Europe, with over 246,000 cases confirmed annually. '' C. jejuni'' infection can also cause bacteremia in immunocompromised people, while '' C. lari'' is a known cause of recurrent diarrhea in children. '' C. fetus'' can cause spontaneous abortions in cattle and sheep, and is an opportunistic pathogen in humans.

Morphology and phenotype

''Campylobacter'' spp. generally appear as curved or comma-shaped rods, and are able to move via unipolar or bipolar flagella. They grow best between 37–42 °C in a microaerophilic environment. When exposed to atmospheric oxygen, ''C. jejuni'' is able to change into a coccus form. Most species of ''Campylobacter'' are positive by the oxidase test and catalase test and are able to reduce nitrate. The number of known quinolone-resistant ''Campylobacter'' strains is growing. It is suggested that this is caused by the overuse of quinolone antibiotics in animal agriculture.

History

Theodor Escherich was the first to describe in 1886 what are known today as ''Campylobacters'' in the stool samples of infants, who perished from a disease he named "cholera infantum". In the following years until the end of the century, a number of publications appeared, describing the occurrence of such "spirilla" in cases of "cholera-like" and "dysenteric" disease. These organisms were mainly found in the colon or associated with mucus in diarrhoeal stool specimens. ''Vibrio''-like bacteria were also described by Sir John McFadyean and Stockman in 1913 in fetal tissues of aborted sheep. For several years Campylobacters were continuously referred to as ‘‘Vibrio-like organisms’’, until 1963 when Sebald and Veron gave the name "''Campylobacter''" to the genus based on their shape and microaerophilic growth requirement and after showing significant biological differences with ''Vibrio'' species.

Genomics

The genomes of several ''Campylobacter'' species have been sequenced, beginning with ''C. jejuni'' in 2000. These genome studies have identified molecular markers specific to members of ''Campylobacter''. ''Campylobacter'' ssp. genomes are rather small compared to those of other gastrointestinal pathogens, with sizes ranging between 1.60 and 1.90 Mbp. A characteristic of most ''Campylobacter'' genomes is the presence of hypervariable regions, which can differ greatly between different strains.

Studies have investigated the genes responsible for motility in ''Campylobacter'' species. Some ''Campylobacter'' species contain two flagellin genes in tandem for motility, ''flaA'' and ''flaB''. These genes undergo intergenic recombination, further contributing to their virulence. A single Type VI secretion system (T6SS) cluster was also predicted in approximately one-third of ''Campylobacter'' species, grouping into three distinct organisations and harbouring up to five ''vgrG'' genes.

Phylogeny

The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and National Center for Biotechnology Information (NCBI)

Species incertae sedis:
* ''C. devanensis'' Miller et al. 2024
* "''C. faecalis''" Kachler et al. 2000
* "''C. lawrenceae''" Foley et al. 2004
* ''C. magnus'' Gruntar et al. 2023
* ''C. porcelli'' Miller et al. 2024
* ''C. vicugnae'' Miller et al. 2024

''Campylobacter''-specific bacteriophages

''Campylobacter''-specific bacteriophages are natural viral predators of the organism. Bacteriophages specific to the species now known as ''C. coli'' and ''C. fetus ''(previously ''Vibrio coli'' and ''V. fetus''), were first isolated from cattle and pigs during the 1960s, and ''Campylobacter'' bacteriophage therapy is an ongoing area of research in the age of bacterial antibiotic resistance.

Pathogenesis

''Campylobacter'' can cause a gastrointestinal infection, campylobacteriosis. The incubation period is 24–72 hours after infection. This is characterized by an inflammatory, sometimes bloody diarrhea or dysentery syndrome, mostly including cramps, fever, and pain. The most common routes of transmission are fecal-oral, ingestion of contaminated food or water, and the eating of raw meat. Foods implicated in campylobacteriosis include raw or under-cooked poultry, raw dairy products, and contaminated produce. ''Campylobacter'' is sensitive to the stomach's normal production of hydrochloric acid: as a result, the infectious dose is relatively high, and the bacteria rarely cause illness when a person is exposed to less than 10,000 organisms. Nevertheless, people taking antacid medication (e. g. people with gastritis or stomach ulcers) are at higher risk of contracting disease from a smaller number of organisms, since this type of medication neutralizes normal gastric acid.

In humans, the sites of tissue injury include the jejunum, the ileum, and the colon. Most strains of ''C. jejuni'' produce cytolethal distending toxin, which inhibits cell division and impedes activation of the immune system. This helps the bacteria to evade the immune system and survive for a limited time inside intestinal cells. ''Campylobacter'' has, on rare occasions, been suggested to cause hemolytic uremic syndrome and thrombotic thrombocytopenic purpura, though no unequivocal case reports exist. ''Campylobacter'' infection is the most common trigger of Guillain–Barré syndrome. Gastrointestinal perforation is a rare complication of ileal infection.

''Campylobacter'' has also been associated with periodontitis.

Detection

Campylobacter testing needs to be done to manage the risk of foodborne ''Campylobacter'' and reducing the level of foodborne ''Campoboteriosis'', to protect people and to determine if a person is infected with ''Campylobacter.''

In humans

Usually, detection of ''Campylobacter'' in humans is done by laboratory culturing a stool sample or swab of the rectum collected by a healthcare provider. Results take about 48–72 hours for preliminary results. Confirmation test and testing to determine the species of ''Campylobacter'' or drug sensitivities of the organism require additional time.

In livestock

Usually, detection of Campylobacter in livestock is done by laboratory culturing a faecal sample. Results take about 48–72 hours.

In meat

Usually, detection of Campylobacter in meat is done by laboratory culturing a homogenised sample. Results takes about 48–72 hours.

Treatment

The infection is usually self-limiting and, in most cases, symptomatic treatment by liquid and electrolyte replacement is sufficient to treat human infections. Symptoms typically last 5–7 days. Treatment with antibiotics has only a minor effect on the typical duration of the infection in non-complex cases, and is discouraged except in high-risk patients.
Diagnosis of campylobacteriosis is made by testing a fecal specimen. Standard treatment in high-risk cases is azithromycin, a macrolide antibiotic, especially for ''Campylobacter'' infections in children, although other antibiotics, such as quinolones, tetracycline and other macrolides are sometimes used to treat gastrointestinal ''Campylobacter'' infections in adults. In case of systemic infection, other bactericidal antibiotics are used, such as ampicillin, amoxicillin/clavulanic acid, or aminoglycosides. Fluoroquinolone antibiotics, such as ciprofloxacin or levofloxacin, may no longer be effective in some cases, due to resistance. In addition to antibiotics, dehydrated patients may require intravenous fluid treatment in a hospital.

Epidemiology

Canada

FoodNet Canada has reported that ''Campylobacter'' was the most common pathogen found on packaged chicken breast, with nearly half of all samples testing positive. Additionally, ''Campylobacter'' and ''Salmonella'' were the most common causes of gastrointestinal illness in Canada.

Italy

In Italy, the annual prevalence of ''Campylobacter'' infections appears to be relatively stable based on findings from a national survey conducted on more than 5000 isolates. The survey revealed that the most common species of ''Campylobacter'' were ''C. jejuni'', accounting for 83.7% of isolates, followed by ''C. coli'' (13.5%) and ''C. fetus'' (0.6%). The mean age of affected patients was 34.61 years, with males constituting 57.1% of cases. Outpatients represented the majority of cases, comprising 54% of the total. ''Campylobacter'' infections were predominantly isolated from feces, accounting for 97.3% of cases, while a smaller proportion (2.7%) was isolated from blood. Notably, ''C. fetus'' was primarily isolated from blood samples, constituting 88.2% of cases. Regarding antibiotic resistance patterns, the survey found that resistance to ciprofloxacin and tetracyclines was relatively high, with rates of 75.5% and 54.8%, respectively. In contrast, resistance to macrolides, including erythromycin, clarithromycin, and azithromycin, was lower, with rates ranging from 2% to 4.8%. Additionally, approximately 50% of ''C. jejuni'' and ''C. coli'' isolates exhibited resistance to two or more antibiotics. There was a significant decrease in resistance to ciprofloxacin and tetracyclines over time, while resistance to macrolides remained stable.

New Zealand

In August 2016, an estimated 8,000+ residents of Havelock North, a town with around 13,000 residents, had gastric illness after the water supply was thought to be contaminated by ''Campylobacter''.

Norway

In June 2019, an estimated 2,000 residents of Askøy municipality got sick due to the presence of ''C. jejuni'' in the water supply. Two deaths were connected to the outbreak, and it was the largest outbreak of ''Campylobacter'' in Norway. The suspected source of the contamination was thought to be horse faeces, which leaked into a drinking water pool. A ''C. jejuni'' water isolate thought to be the cause of the outbreak was examined with human isolates, and showed the highest pathogenic potential in vitro, transcriptomic and genomic investigations. This could suggest why the isolate was able to cause an outbreak.

Sweden

During the period of August 2016 to June 2017 there was a large outbreak of ''C. jejuni'' in Sweden. It was the largest outbreak that has been reported so far. 5000 more cases than would be expected during this period were reported to the authorities. The source of the outbreak was contaminated chicken meat that came from the same producer. The reason for the increased incidence and elevated levels of ''Campylobacter'' was reported to be an improperly installed washing plant, where dirty water was accidentally used to wash transport cages.

United Kingdom

In January 2013, the UK's Food Standards Agency (FSA) warned that two-thirds of all raw chicken bought from UK shops was contaminated with ''Campylobacter'', affecting an estimated half a million people annually and killing about 100 of them. In June 2014, the FSA started a campaign against washing raw chicken, as washing can spread germs onto clean surfaces by splashing. In May 2015, cumulative results for samples taken from fresh chickens between February 2014 and February 2015 were published by the FSA and showed 73% of chickens tested positive for the presence of ''Campylobacter''.

United States

''Campylobacter'' infections increased 14% in the United States in 2012 compared to the rate from 2006 to 2008. This represents the highest reported number of infections since calendar year 2000.

High prevalence of ''Campylobacter'' (40% or more) has been reported in raw chicken meat in regional retail stores in the US, which remained steady from 2005 through 2011. The last USDA quarterly progress report on ''Salmonella'' and ''Campylobacter'' testing of meat and poultry, for July–September 2014, showed a low prevalence of ''Campylobacter'' spp. in ground chicken meat, but a larger prevalence (20%) in mechanically separated chicken meat (which is sold only for further processing).

See also

* '' Helicobacter''
* List of bacterial orders
* List of bacteria genera

References

External links

WHO fact sheet on ''Campylobacter''

''Campylobacter'' info from the CDC

{{Authority control

Bacteria genera
Campylobacterota
Capnophiles
Food microbiology
Pathogenic bacteria
Zoonoses