Single-strand Conformation Polymorphism
Single-strand conformation polymorphism (SSCP), or single-strand ''chain'' polymorphism, is defined as a conformational difference of single-stranded nucleotide sequences of identical length as induced by differences in the sequences under certain experimental conditions. This property allows sequences to be distinguished by means of gel electrophoresis, which separates fragments according to their different conformations. Physical background A single nucleotide change in a particular sequence, as seen in a double-stranded DNA, cannot be distinguished by gel electrophoresis techniques, which can be attributed to the fact that; the physical properties of the double strands are almost identical for both alleles. After denaturation, single-stranded DNA undergoes a characteristic 3-dimensional folding and may assume a unique conformational state based on its DNA sequence. The difference in shape between two single-stranded DNA strands with different sequences can cause them to migr ...
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