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Filler DNA
Carrier DNA is chemically inert, fragmented DNA used in molecular biology during extraction and precipitation procedures to improve the recovery of trace amounts of specific DNA or RNA sequences.Sambrook, Joseph; Russell, David W. (2001). ''Molecular Cloning: A Laboratory Manual'' (3rd ed.). Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press. pp. A1.20–A1.21. . It is typically added to a solution containing the target nucleic acid before precipitation. The carrier DNA acts as a co-precipitant, adding bulk to the sample and forming a visible pellet that helps to trap the desired, low-concentration nucleic acid molecules, thus preventing their loss. Since carrier DNA is used as a reagent, it is not intended to be replicated or expressed by a cell. Carrier DNA applications include the extraction of DNA from forensic samples like hair or trace evidence; the purification of viral DNA or RNA from clinical samples with low viral titers, such as plasma; the isolation of cell-fr ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cloning Vector
A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium. The vector contains features that allow for the convenient insertion of a DNA fragment into the vector or its removal from the vector, for example through the presence of restriction sites. The vector and the foreign DNA may be treated with a restriction enzyme that cuts the DNA, and DNA fragments thus generated contain either blunt ends or overhangs known as sticky ends, and vector DNA and foreign DNA with compatible ends can then be joined by molecular ligation. After a DNA fragment has been cloned into a cloning vector, it may be further subcloned into another vector designed for more specific use. There are many types of cloning vectors, but the most commonly used ones are genetically engineer ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
