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Excitation Filter
An excitation filter is a high quality optical-glass filter commonly used in fluorescence microscopy and spectroscopic applications for selection of the excitation wavelength of light from a light source. Most excitation filters select light of relatively short wavelengths from an excitation light source, as only those wavelengths would carry enough energy to cause the object the microscope is examining to fluoresce sufficiently. The excitation filters used may come in two main types — ''short pass filters'' and '' band pass filters''. Variations of these filters exist in the form of ''notch filters'' or deep blocking filters (commonly employed as emission filters). Other forms of excitation filters include the use of monochromators, wedge prisms coupled with a narrow slit (for selection of the excitation light) and the use of holographic diffraction grating In optics, a diffraction grating is an optical grating with a periodic structure that diffraction, diffracts light ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescence Microscope
A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. A fluorescence microscope is any microscope that uses fluorescence to generate an image, whether it is a simple setup like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image. Principle The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light). The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter. Typical components of a fluorescence microscope are a light source (xenon arc lamp or mercury-vapor lamp are com ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spectroscopy
Spectroscopy is the field of study that measures and interprets electromagnetic spectra. In narrower contexts, spectroscopy is the precise study of color as generalized from visible light to all bands of the electromagnetic spectrum. Spectroscopy, primarily in the electromagnetic spectrum, is a fundamental exploratory tool in the fields of astronomy, chemistry, materials science, and physics, allowing the composition, physical structure and electronic structure of matter to be investigated at the atomic, molecular and macro scale, and over astronomical distances. Historically, spectroscopy originated as the study of the wavelength dependence of the absorption by gas phase matter of visible light dispersed by a prism. Current applications of spectroscopy include biomedical spectroscopy in the areas of tissue analysis and medical imaging. Matter waves and acoustic waves can also be considered forms of radiative energy, and recently gravitational waves have been associa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Planck Relation
The Planck relationFrench & Taylor (1978), pp. 24, 55.Cohen-Tannoudji, Diu & Laloë (1973/1977), pp. 10–11. (referred to as Planck's energy–frequency relation,Schwinger (2001), p. 203. the Planck–Einstein relation, Planck equation, and Planck formula, though the latter might also refer to Planck's law) is a fundamental equation in quantum mechanics which states that the energy of a photon, known as photon energy, is proportional to its frequency : E = h \nu. The constant of proportionality, , is known as the Planck constant. Several equivalent forms of the relation exist, including in terms of angular frequency : E = \hbar \omega, where \hbar = h / 2 \pi. Written using the symbol for frequency, the relation is E = h f. The relation accounts for the quantized nature of light and plays a key role in understanding phenomena such as the photoelectric effect and black-body radiation (where the related Planck postulate can be used to derive Planck's law). Spectral form ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Band Pass Filter
A band-pass filter or bandpass filter (BPF) is a device that passes frequencies within a certain range and rejects (attenuates) frequencies outside that range. It is the inverse of a ''band-stop filter''. Description In electronics and signal processing, a filter is usually a two-port circuit or device which removes frequency components of a signal (an alternating voltage or current). A band-pass filter allows through components in a specified band of frequencies, called its ''passband'' but blocks components with frequencies above or below this band. This contrasts with a high-pass filter, which allows through components with frequencies above a specific frequency, and a low-pass filter, which allows through components with frequencies below a specific frequency. In digital signal processing, in which signals represented by digital numbers are processed by computer programs, a band-pass filter is a computer algorithm that performs the same function. The term band-pa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Notch Filter
In signal processing, a band-stop filter or band-rejection filter is a filter that passes most frequencies unaltered, but attenuates those in a specific range to very low levels. It is the inverse of a ''band-pass filter''. A notch filter is a band-stop filter with a narrow stopband (high Q factor). Narrow notch filters (optical) are used in Raman spectroscopy, live sound reproduction (public address systems, or PA systems) and in instrument amplifiers (especially amplifiers or preamplifiers for acoustic instruments such as acoustic guitar, mandolin, bass instrument amplifier, etc.) to reduce or prevent audio feedback, while having little noticeable effect on the rest of the frequency spectrum ( electronic or software filters). Other names include "band limit filter", "T-notch filter", "band-elimination filter", and "band-reject filter". Typically, the width of the stopband is 1 to 2 decades (that is, the highest frequency attenuated is 10 to 100 times the lowest frequency ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Emission Filters
Emission may refer to: Chemical products * Emission of air pollutants, notably: ** Flue gas, gas exiting to the atmosphere via a flue ** Exhaust gas, flue gas generated by fuel combustion ** Emission of greenhouse gases, which absorb and emit radiant energy within the thermal infrared range * Emission standards, limits on pollutants that can be released into the environment * Emissions trading, a market-based approach to pollution control Electromagnetic radiation * Emission spectrum, the spectrum of frequencies of electromagnetic radiation generated by molecular electrons making transitions to lower energy states * Thermal emission, electromagnetic radiation generated by the thermal motion of particles in matter * List of light sources, including both natural and artificial processes that emit light * Emission (radiocommunications), a radio signal (usually modulated) emitted from a radio transmitter * Emission coefficient, a coefficient in the power output per unit time of an el ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Monochromator
A monochromator is an optics, optical device that transmits a mechanically selectable narrow band of wavelengths of light or other radiation chosen from a wider range of wavelengths available at the input. The name is . Uses A device that can produce monochromatic light has many uses in science and in optics because many optical characteristics of a material are dependent on wavelength. Although there are a number of useful ways to select a narrow band of wavelengths (which, in the visible range, is perceived as a pure color), there are not as many other ways to easily select any wavelength band from a wide range. See #Applications, below for a discussion of some of the uses of monochromators. In hard X-ray and neutron radiation, neutron optics, crystal monochromators are used to define wave conditions on the instruments. Techniques A monochromator can use either the phenomenon of optical dispersion in a Prism (optics), prism, or that of diffraction using a diffraction gratin ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Wedge Prism
The wedge prism is a prism with a shallow angle between its input and output surfaces. This angle is usually 3 degrees or less. Refraction at the surfaces causes the prism to deflect light by a fixed angle. When viewing a scene through such a prism, objects will appear to be offset by an amount that varies with their distance from the prism. Deflection angle For a wedge prism in air, rays of light passing through the prism are deflected by the angle δ, which is approximately given by :\delta \approx (n - 1) \alpha\ , where ''n'' is the index of refraction of the prism material, and α is the angle between the prism's surfaces. Applications The term "optical wedge" refers to any shallow angle between two plane surfaces of a window. This wedge may range from a few millionths of a degree of perfect parallelism to as much as three degrees of angle. Even though high-precision optics, such as optical flats, may be lapped and polished to extremely high levels of parallelism ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Holographic
Holography is a technique that allows a wavefront to be recorded and later reconstructed. It is best known as a method of generating three-dimensional images, and has a wide range of other uses, including data storage, microscopy, and interferometry. In principle, it is possible to make a hologram for any type of Holography#Non-optical holography, wave. A hologram is a recording of an Wave interference, interference pattern that can reproduce a 3D light field using diffraction. In general usage, a hologram is a recording of any type of wavefront in the form of an interference pattern. It can be created by capturing light from a real scene, or it can be generated by a computer, in which case it is known as a computer-generated hologram, which can show virtual objects or scenes. Optical holography needs a laser light to record the light field. The reproduced light field can generate an image that has the depth and parallax of the original scene. A hologram is usually unintelligi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Diffraction Grating
In optics, a diffraction grating is an optical grating with a periodic structure that diffraction, diffracts light, or another type of electromagnetic radiation, into several beams traveling in different directions (i.e., different diffraction angles). The emerging coloration is a form of structural coloration. The directions or diffraction angles of these beams depend on the wave (light) Angle of incidence (optics), incident angle to the diffraction grating, the spacing or periodic distance between adjacent diffracting elements (e.g., parallel slits for a transmission grating) on the grating, and the wavelength of the incident light. The grating acts as a dispersion (optics), dispersive element. Because of this, diffraction gratings are commonly used in monochromators and spectrometers, but other applications are also possible such as optical encoders for high-precision motion control and wavefront measurement. For typical applications, a reflection (optics), reflective grati ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Beam Diffraction
Beam may refer to: Streams of particles or energy *Light beam, or beam of light, a directional projection of light energy **Laser beam *Radio beam *Particle beam, a stream of charged or neutral particles **Charged particle beam, a spatially localized group of electrically charged particles ***Cathode ray, or electron beam or e-beam, streams of electrons observed in discharge tubes ***X-ray beam, a penetrating form of high-energy electromagnetic radiation **Molecular beam, a beam of particles moving at approximately equal velocities People * Beam (rapper) (born 1995), American hip hop artist * Anong Beam, Canadian Ojibwe artist and curator * Carl Beam (1943–2005), Indigenous Canadian artist Arts, entertainment and media * Beam (music), a connection line in musical notation * ''Beam'' (single album), by Hoshi X Woozi, 2025 * ''The Beam'' (fairy tale), the Brothers Grimm tale 149 * BEAM.TV, an online digital delivery and content management platform * BEAM Channel 31, a Ph ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dichroic
In optics, a dichroic material is either one which causes visible light to be split up into distinct beams of different wavelengths (colours) (not to be confused with dispersion), or one in which light rays having different polarizations are absorbed by different amounts. In beam splitters The original meaning of ''dichroic'', from the Greek ''dikhroos'', two-coloured, refers to any optical device which can split a beam of light into two beams with differing wavelengths. Such devices include mirrors and filters, usually treated with optical coatings, which are designed to reflect light over a certain range of wavelengths and transmit light which is outside that range. An example is the dichroic prism, used in some camcorders, which uses several coatings to split light into red, green and blue components for recording on separate CCD arrays, however it is now more common to have a Bayer filter to filter individual pixels on a single CCD array. This kind of dichroic device does ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
