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Xanthomonas SRNA
In molecular biology, ''Xanthomonas'' sRNA are small RNAs which have been identified in various species of the bacterium '' Xanthomonas''. Analysis of the plant pathogen '' Xanthomonas campestris pv. vesicatoria'' revealed expression of seven cis-encoded antisense RNAs (asX1-asX7) and 15 intergenic sRNAs (sX1 - sX15). Several sRNAs have also been identified in '' Xanthomonas campestris pathovar campestris''. Some of these ''X. campestris'' sRNAs are only found in ''Xanthomonas'', some are also expressed in other bacteria. Several of these sRNAs appear to contribute to virulence, including sX12, sX13 and sRNA-Xcc1. Computational analysis predicts 63 sRNAs in '' Xanthomonas oryzae pathovar oryzae'', expression of 8 of these has been experimentally confirmed. Expression of three of these is Hfq-dependent. See also * ''Bacillus subtilis'' BSR sRNAs *Bacterial small RNA Bacterial small RNAs (bsRNA) are small RNAs produced by bacteria; they are 50- to 500-nucleotide non-coding RNA m ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bacterial Small RNA
Bacterial small RNAs (bsRNA) are small RNAs produced by bacteria; they are 50- to 500-nucleotide non-coding RNA molecules, highly structured and containing several stem-loops. Numerous sRNAs have been identified using both computational analysis and laboratory-based techniques such as Northern blotting, microarrays and RNA-Seq in a number of bacterial species including ''Escherichia coli'', the model pathogen ''Salmonella'', the nitrogen-fixing alphaproteobacterium '' Sinorhizobium meliloti'', marine cyanobacteria, '' Francisella tularensis'' (the causative agent of tularaemia), ''Streptococcus pyogenes','' the pathogen ''Staphylococcus aureus'''','' and the plant pathogen '' Xanthomonas oryzae pathovar oryzae''. Bacterial sRNAs affect how genes are expressed within bacterial cells via interaction with mRNA or protein, and thus can affect a variety of bacterial functions like metabolism, virulence, environmental stress response, and structure. Origin In the 1960s, the abbreviatio ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bacterium
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic r ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Xanthomonas
''Xanthomonas'' (from greek: ''xanthos'' – “yellow”; ''monas'' – “entity”) is a genus of bacteria, many of which cause plant diseases. There are at least 27 plant associated ''Xanthomonas spp.'', that all together infect at least 400 plant species. Different species typically have specific host and/or tissue range and colonization strategies. Taxonomy The genus ''Xanthomonas'' has been subject of numerous taxonomic and phylogenetic studies and was first described as ''Bacterium vesicatorium'' as a pathogen of pepper and tomato in 1921. Dowson later reclassified the bacterium as ''Xanthomonas campestris'' and proposed the genus ''Xanthomonas''.''Xanthomonas'' was first described as a monotypic genus and further research resulted in the division into two groups, A and B. Later work using DNA:DNA hybridization has served as a framework for the general ''Xanthomonas'' species classification. Other tools, including multilocus sequence analysis and amplified fragment-length ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Plant Pathogen
Plant pathology (also phytopathology) is the scientific study of diseases in plants caused by pathogens (infectious organisms) and environmental conditions (physiological factors). Organisms that cause infectious disease include fungi, oomycetes, bacteria, viruses, viroids, virus-like organisms, phytoplasmas, protozoa, nematodes and parasitic plants. Not included are ectoparasites like insects, mites, vertebrate, or other pests that affect plant health by eating plant tissues. Plant pathology also involves the study of pathogen identification, disease etiology, disease cycles, economic impact, plant disease epidemiology, plant disease resistance, how plant diseases affect humans and animals, pathosystem genetics, and management of plant diseases. Overview Control of plant diseases is crucial to the reliable production of food, and it provides significant problems in agricultural use of land, water, fuel and other inputs. Plants in both natural and cultivated ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Xanthomonas Campestris Pv
''Xanthomonas'' (from greek: ''xanthos'' – “yellow”; ''monas'' – “entity”) is a genus of bacteria, many of which cause plant diseases. There are at least 27 plant associated ''Xanthomonas spp.'', that all together infect at least 400 plant species. Different species typically have specific host and/or tissue range and colonization strategies. Taxonomy The genus ''Xanthomonas'' has been subject of numerous taxonomic and phylogenetic studies and was first described as ''Bacterium vesicatorium'' as a pathogen of pepper and tomato in 1921. Dowson later reclassified the bacterium as ''Xanthomonas campestris'' and proposed the genus ''Xanthomonas''.''Xanthomonas'' was first described as a monotypic genus and further research resulted in the division into two groups, A and B. Later work using DNA:DNA hybridization has served as a framework for the general ''Xanthomonas'' species classification. Other tools, including multilocus sequence analysis and amplified fragment-lengt ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Antisense
In molecular biology and genetics, the sense of a nucleic acid molecule, particularly of a strand of DNA or RNA, refers to the nature of the roles of the strand and its complement in specifying a sequence of amino acids. Depending on the context, sense may have slightly different meanings. For example, negative-sense strand of DNA is equivalent to the template strand, whereas the positive-sense strand is the non-template strand whose nucleotide sequence is equivalent to the sequence of the mRNA transcript. DNA sense Because of the complementary nature of base-pairing between nucleic acid polymers, a double-stranded DNA molecule will be composed of two strands with sequences that are reverse complements of each other. To help molecular biologists specifically identify each strand individually, the two strands are usually differentiated as the "sense" strand and the "antisense" strand. An individual strand of DNA is referred to as positive-sense (also positive (+) or simply sense) ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Small RNA
Small RNA (sRNA) are polymeric RNA molecules that are less than 200 nucleotides in length, and are usually non-coding. RNA silencing is often a function of these molecules, with the most common and well-studied example being RNA interference (RNAi), in which endogenously expressed microRNA (miRNA) or exogenously derived small interfering RNA (siRNA) induces the degradation of complementary messenger RNA. Other classes of small RNA have been identified, including piwi-interacting RNA (piRNA) and its subspecies repeat associated small interfering RNA (rasiRNA). Small RNA "is unable to induce RNAi alone, and to accomplish the task it must form the core of the RNA–protein complex termed the RNA-induced silencing complex (RISC), specifically with Argonaute protein". Small RNA have been detected or sequenced using a range of techniques, including directly by MicroRNA sequencing on several sequencing platforms, or indirectly through genome sequencing and analysis. Identification of ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Virulence
Virulence is a pathogen's or microorganism's ability to cause damage to a host. In most, especially in animal systems, virulence refers to the degree of damage caused by a microbe to its host. The pathogenicity of an organism—its ability to cause disease—is determined by its virulence factors. In the specific context of gene for gene systems, often in plants, virulence refers to a pathogen's ability to infect a resistant host. The noun ''virulence'' derives from the adjective ''virulent'', meaning disease severity. The word ''virulent'' derives from the Latin word ''virulentus'', meaning "a poisoned wound" or "full of poison." From an ecological standpoint, virulence is the loss of fitness induced by a parasite upon its host. Virulence can be understood in terms of proximate causes—those specific traits of the pathogen that help make the host ill—and ultimate causes—the evolutionary pressures that lead to virulent traits occurring in a pathogen strain. Virulent ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
SRNA-Xcc1
sRNA-Xcc1 (small RNA identified from ''Xanthomonas campestris'' pv. ''campestris'') is a family of trans-acting non-coding RNA (also known as small RNA). Homologs of sRNA-Xcc1 are found in a few bacterial strains belonging to alpha-proteobacteria, beta-proteobacteria, gamma-proteobacteria, and delta-proteobacteria. In ''Xanthomonas'' ''campestris'' pv. ''campestris'', sRNA-Xcc1 is encoded by an integron gene cassette and is under the positive control of the virulence regulators HrpG and HrpX. Origin and phylogenetic distribution sRNA-Xcc1 is encoded by a gene cassette in the integron of ''Xanthomonas campestris'' pv. ''campestris'' and homologs of ''sRNA-Xcc1'' are frequently found in integron gene cassettes cloned from uncultured bacterium, it is possible that sRNA-Xcc1 is originally captured by integrons from natural environments. ''sRNA-Xcc1'' homologs are found in a few taxonomically far related strains across alpha-, beta- and gamma-proteobacteria but not in close relat ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Xanthomonas Oryzae Pv
''Xanthomonas'' (from greek: ''xanthos'' – “yellow”; ''monas'' – “entity”) is a genus of bacteria, many of which cause plant diseases. There are at least 27 plant associated ''Xanthomonas spp.'', that all together infect at least 400 plant species. Different species typically have specific host and/or tissue range and colonization strategies. Taxonomy The genus ''Xanthomonas'' has been subject of numerous taxonomic and phylogenetic studies and was first described as ''Bacterium vesicatorium'' as a pathogen of pepper and tomato in 1921. Dowson later reclassified the bacterium as ''Xanthomonas campestris'' and proposed the genus ''Xanthomonas''.''Xanthomonas'' was first described as a monotypic genus and further research resulted in the division into two groups, A and B. Later work using DNA:DNA hybridization has served as a framework for the general ''Xanthomonas'' species classification. Other tools, including multilocus sequence analysis and amplified fragment-length ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Hfq Protein
The Hfq protein (also known as HF-I protein) encoded by the ''hfq'' gene was discovered in 1968 as an ''Escherichia coli'' host factor that was essential for replication of the bacteriophage Qβ. It is now clear that Hfq is an abundant bacterial RNA binding protein which has many important physiological roles that are usually mediated by interacting with Hfq binding sRNA. In ''E. coli'', Hfq mutants show multiple stress response related phenotypes. The Hfq protein is now known to regulate the translation of two major stress transcription factors ( σS (RpoS) and σE (RpoE) ) in Enterobacteria. It also regulates sRNA in ''Vibrio cholerae'', a specific example being MicX sRNA. In ''Salmonella typhimurium'', Hfq has been shown to be an essential virulence factor as its deletion attenuates the ability of ''S.typhimurium'' to invade epithelial cells, secrete virulence factors or survive in cultured macrophages. In ''Salmonella'', Hfq deletion mutants are also non motile and exhibit ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bacillus Subtilis BSR SRNAs
In a screen of the ''Bacillus subtilis'' genome for genes encoding ncRNAs, Saito et al. focused on 123 intergenic regions (IGRs) over 500 base pairs in length, the authors analyzed expression from these regions. Seven IGRs termed bsrC, bsrD, bsrE, bsrF, bsrG, bsrH and bsrI expressed RNAs smaller than 380 nt. All the small RNAs except BsrD RNA were expressed in transformed ''Escherichia coli'' cells harboring a plasmid with PCR-amplified IGRs of ''B. subtilis'', indicating that their own promoters independently express small RNAs. Under non-stressed condition, depletion of the genes for the small RNAs did not affect growth. Although their functions are unknown, gene expression profiles at several time points showed that most of the genes except for bsrD were expressed during the vegetative phase (4–6 h), but undetectable during the stationary phase (8 h). Mapping the 5' ends of the 6 small RNAs revealed that the genes for BsrE, BsrF, BsrG, BsrH, and BsrI RNAs are preceded by a rec ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
