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Tcr-seq
TCR-Seq (T-cell Receptor Sequencing) is a method used to identify and track specific T cells and their clones. TCR-Seq utilizes the unique nature of a T-cell receptor, T-cell receptor (TCR) as a ready-made molecular barcode. This technology can apply to both single cell sequencing technologies and High-throughput screening, high throughput screens Background T-cell Receptor (TCR) T cells are a part of the adaptive immune system and play a critical role in protecting the body from foreign pathogens. T-cell receptor, T-cell receptors (TCRs) are a group of membrane proteins found on the surface of T cells which can bind to foreign antigens. TCRs interact with Major histocompatibility complex, major histocompatibility complexes (MHC) on cell surfaces to recognize antigens. They are heterodimers made up of predominantly α and β chains (or more rarely δ and γ chains) and consist of a variable region and a constant region. Variable regions are produced through a process called V( ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Single Cell Sequencing
Single-cell sequencing examines the nucleic acid Genome sequence, sequence information from individual Cell (biology), cells with optimized next generation sequencing, next-generation sequencing technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment. For example, in cancer, sequencing the DNA of individual cells can give information about mutations carried by small populations of cells. In development, sequencing the RNAs expressed by individual cells can give insight into the existence and behavior of different cell types. In microbial systems, a population of the same species can appear genetically clonal. Still, single-cell sequencing of RNA or Epigenetics, epigenetic modifications can reveal cell-to-cell variability that may help populations rapidly adapt to survive in changing environments. Background A typical human cell consists of about 2 x 3.3 billion base ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
