Staphylococcus Carnosus
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Staphylococcus Carnosus
''Staphylococcus carnosus'' is a bacterium from the genus ''Staphylococcus'' that is both Gram-positive and coagulase-negative. It was originally identified in dry sausage and is an important starter culture for meat fermentation. Unlike other members of its genus, such as ''Staphylococcus aureus'' and ''Staphylococcus epidermidis'', ''S. carnosus'' is nonpathogenic and safely used in the food industry. Taxonomy ''Staphylococcus carnosus'' is classified under the domain Bacteria, phylum Bacillota, class Bacilli, order Bacillales, family Staphylococcaceae, genus ''Staphylococcus'', and species ''S. carnosus.'' The ''Staphylococcus'' genus currently comprises over 60 species and subspecies, including ''S. carnosus.'' Phylogeny PCR and DNA sequencing along with 16s rRNA sequencing are commonly used to differentiate among the staphylococcal species. Different methods of phylogenetic analysis have been used to examine the relationships between the different ''Staphylococcus'' ...
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Gram-positive Bacteria
In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. The Gram stain is used by microbiologists to place bacteria into two main categories, gram-positive (+) and gram-negative (−). Gram-positive bacteria have a thick layer of peptidoglycan within the cell wall, and gram-negative bacteria have a thin layer of peptidoglycan. Gram-positive bacteria retain the crystal violet stain used in the test, resulting in a purple color when observed through an optical microscope. The thick layer of peptidoglycan in the bacterial cell wall retains the stain after it has been fixed in place by iodine. During the decolorization step, the decolorizer removes crystal violet from all other cells. Conversely, gram-negative bacteria cannot retain the violet stain after the decolorization step; alcohol used in this stage ...
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Phylogenetics
In biology, phylogenetics () is the study of the evolutionary history of life using observable characteristics of organisms (or genes), which is known as phylogenetic inference. It infers the relationship among organisms based on empirical data and observed heritable traits of DNA sequences, protein amino acid sequences, and morphology. The results are a phylogenetic tree—a diagram depicting the hypothetical relationships among the organisms, reflecting their inferred evolutionary history. The tips of a phylogenetic tree represent the observed entities, which can be living taxa or fossils. A phylogenetic diagram can be rooted or unrooted. A rooted tree diagram indicates the hypothetical common ancestor of the taxa represented on the tree. An unrooted tree diagram (a network) makes no assumption about directionality of character state transformation, and does not show the origin or "root" of the taxa in question. In addition to their use for inferring phylogenetic pa ...
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Maltose
} Maltose ( or ), also known as maltobiose or malt sugar, is a disaccharide formed from two units of glucose joined with an α(1→4) bond. In the isomer isomaltose, the two glucose molecules are joined with an α(1→6) bond. Maltose is the two-unit member of the amylose homologous series, the key structural motif of starch. When beta-amylase breaks down starch, it removes two glucose units at a time, producing maltose. An example of this reaction is found in germinating seeds, which is why it was named after malt. Unlike sucrose, it is a reducing sugar. History Maltose was discovered by Augustin-Pierre Dubrunfaut, although this discovery was not widely accepted until it was confirmed in 1872 by Irish chemist and brewer Cornelius O'Sullivan. Its name comes from malt, combined with the suffix ' -ose' which is used in names of sugars. Structure and nomenclature Carbohydrates are generally divided into monosaccharides, oligosaccharides, and polysaccharides depending on th ...
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Sucrose
Sucrose, a disaccharide, is a sugar composed of glucose and fructose subunits. It is produced naturally in plants and is the main constituent of white sugar. It has the molecular formula . For human consumption, sucrose is extracted and refined from either sugarcane or sugar beet. Sugar mills – typically located in tropical regions near where sugarcane is grown – crush the cane and produce raw sugar which is shipped to other factories for refining into pure sucrose. Sugar beet factories are located in temperate climates where the beet is grown, and process the beets directly into refined sugar. The Sugar refinery, sugar-refining process involves washing the raw sugar crystals before dissolving them into a sugar syrup which is filtered and then passed over carbon to remove any residual colour. The sugar syrup is then concentrated by boiling under a vacuum and crystallized as the final purification process to produce crystals of pure sucrose that are clear, odorless, and sweet. ...
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Acetoin
Acetoin, also known as 3-hydroxybutanone or acetyl methyl carbinol, is an organic compound with the formula CH3CH(OH)C(O)CH3. It is a colorless liquid with a pleasant, buttery odor. It is chiral. The form produced by bacteria is (''R'')-acetoin.Albert Gossauer: ''Struktur und Reaktivität der Biomoleküle'', Verlag Helvetica Chimica Acta, Zürich, 2006, Seite 285, . Production in bacteria Acetoin is a neutral, four-carbon molecule used as an external energy store by a number of fermentative bacteria. It is produced by the decarboxylation of alpha- acetolactate, a common precursor in the biosynthesis of branched-chain amino acids. Owing to its neutral nature, production and excretion of acetoin during exponential growth prevents over-acidification of the cytoplasm and the surrounding medium that would result from accumulation of acidic metabolic products, such as acetic acid and citric acid. Once superior carbon sources are exhausted, and the culture enters stationary phase, ac ...
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Nitrate
Nitrate is a polyatomic ion with the chemical formula . salt (chemistry), Salts containing this ion are called nitrates. Nitrates are common components of fertilizers and explosives. Almost all inorganic nitrates are solubility, soluble in water. An example of an insoluble nitrate is bismuth oxynitrate. Chemical structure The nitrate anion is the conjugate acid, conjugate base of nitric acid, consisting of one central nitrogen atom surrounded by three identically bonded oxygen atoms in a trigonal planar arrangement. The nitrate ion carries a formal charge of −1. This charge results from a combination formal charge in which each of the three oxygens carries a − charge, whereas the nitrogen carries a +1 charge, all these adding up to formal charge of the polyatomic nitrate ion. This arrangement is commonly used as an example of Resonance (chemistry), resonance. Like the isoelectronic carbonate ion, the nitrate ion can be represented by three resonance structures: Che ...
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Phylogenetic Tree
A phylogenetic tree or phylogeny is a graphical representation which shows the evolutionary history between a set of species or taxa during a specific time.Felsenstein J. (2004). ''Inferring Phylogenies'' Sinauer Associates: Sunderland, MA. In other words, it is a branching diagram or a tree showing the evolutionary relationships among various biological species or other entities based upon similarities and differences in their physical or genetic characteristics. In evolutionary biology, all life on Earth is theoretically part of a single phylogenetic tree, indicating common ancestry. Phylogenetics is the study of phylogenetic trees. The main challenge is to find a phylogenetic tree representing optimal evolutionary ancestry between a set of species or taxa. Computational phylogenetics (also phylogeny inference) focuses on the algorithms involved in finding optimal phylogenetic tree in the phylogenetic landscape. Phylogenetic trees may be rooted or unrooted. In a ''rooted'' p ...
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Staphylococcus Pseudintermedius
''Staphylococcus pseudintermedius'' is a gram-positive spherically shaped bacterium of the genus ''Staphylococcus'' found worldwide. It is primarily a pathogen for domestic animals, but has been known to affect humans as well. ''S. pseudintermedius'' is an opportunistic pathogen that secretes immune-modulating virulence factors, has many adhesion factors, and the potential to create biofilms, all of which help to determine the pathogenicity of the bacterium. Diagnoses of ''S. pseudintermedius'' have traditionally been made using cytology, plating, and biochemical tests. More recently, molecular technologies like MALDI-TOF, DNA hybridization and PCR have become preferred over biochemical tests for their more rapid and accurate identifications. This includes the identification and diagnosis of antibiotic resistant strains. Morphology and classification Staphylococci spp. are a genus of gram positive cocci of 0.5 - 1 μm diameter. ''Staphylococcus pseudintermedius'' is a no ...
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Novobiocin
Novobiocin, also known as albamycin, is an aminocoumarin antibiotic that is produced by the actinomycete ''Streptomyces niveus'', which has recently been identified as a subjective synonym for ''S. spheroides'' a member of the class Actinomycetia. Other aminocoumarin antibiotics include clorobiocin and coumermycin A1. Novobiocin was first reported in the mid-1950s (then called streptonivicin). Clinical use It is active against ''Staphylococcus epidermidis'' and may be used to differentiate it from the other coagulase-negative ''Staphylococcus saprophyticus'', which is resistant to novobiocin, in culture. Novobiocin was licensed for clinical use under the tradename Albamycin (Upjohn) in the 1960s. Its efficacy#Pharmacology, efficacy has been demonstrated in Clinical trial#Pre clinical studies, preclinical and Clinical trial, clinical trials. The oral form of the drug has since been withdrawn from the market due to lack of efficacy. A combination product of novobiocin and tetrac ...
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Staphylococcus Piscifermentans
''Staphylococcus piscifermentans'' is a Gram-positive, coagulase-negative member of the bacterial genus'' Staphylococcus'' consisting of clustered cocci. This species was originally isolated from fermented fish in Thailand. A later study found a strain of ''S. piscifermentans'' in dog feces. The species is used in the preparation of fermented foods along with ''Staphylococcus carnosus''; both species reduce nitrate and produce ammonia Ammonia is an inorganic chemical compound of nitrogen and hydrogen with the chemical formula, formula . A Binary compounds of hydrogen, stable binary hydride and the simplest pnictogen hydride, ammonia is a colourless gas with a distinctive pu .... References External linksUniProt taxonomyType strain of ''Staphylococcu ...
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Staphylococcus Condimenti
''Staphylococcus condimenti'' is a Gram-positive, coagulase-negative member of the bacterial genus ''Staphylococcus'' consisting of single, paired, and clustered cocci. Strains of this species were originally isolated from fermenting soy sauce mash and are positive for catalase, urease, arginine dihydrolase, nitrate reductase, beta-galactosidase, and phosphatase activity. Unlike some clinical ''Staphylococcus'' isolates and some food-derived strains, ''S. condimenti'' has shown no noticeable resistance to antibiotics including lincomycin and penicillin Penicillins (P, PCN or PEN) are a group of beta-lactam antibiotic, β-lactam antibiotics originally obtained from ''Penicillium'' Mold (fungus), moulds, principally ''Penicillium chrysogenum, P. chrysogenum'' and ''Penicillium rubens, P. ru .... References External linksType strain of ''Staphylococcus condimenti'' at Bac''Dive'' - the Bacterial Diversity Metadatabase condimenti Bacteria described in 1998 { ...
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Staphylococcus Simulans
''Staphylococcus simulans'' is a Gram-positive, coagulase-negative member of the bacterial genus ''Staphylococcus'' consisting of single, paired, and clustered cocci Bacterial cellular morphologies are the shapes that are characteristic of various types of bacteria and often key to their identification. Their direct examination under a light microscope enables the classification of these bacteria (and archaea .... References External linksType strain of ''Staphylococcus simulans'' at Bac''Dive'' - the Bacterial Diversity Metadatabase simulans Bacteria described in 1975 {{Staphylococcaceae-stub ...
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