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Sexing
Sexing or gender identification is the process of determining the sex of an individual animal. Through sexing, biologists and agricultural workers determine the sex of livestock and other animals they work with. The specialized trade of chicken sexing has a particular importance in the poultry industry. The sex of mammals can often be determined using sexually dimorphic characteristics. Assisted physical sexing is relevant in vertebrates with cloacae (e.g. birds, reptiles or amphibians) when there is no external sexual dimorphism. In veterinary practice, fibroscopy is used under general anaesthesia in birds such as parrots. Molecular sexing is a set of techniques that use DNA for determining sex in wild or domestic species (population studies, farming, genetics) or humans (archaeology, forensic medicine). Markers commonly used include amelogenin, SRY and ZFX/ ZFY. Various techniques have been developed using simple polymerase chain reaction The polymerase chain reacti ...
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Chick Sexing
Chick sexing is the method of distinguishing the sex of chickens and other hatchlings, usually by a trained person called a chick sexer or chicken sexer. Chicken sexing is practiced mostly by large commercial hatcheries to separate female chicks or "pullets" (destined to lay eggs for commercial sale) from the males or " cockerels" (most of which are killed within days of hatching because they are irrelevant to egg production). The females and a limited number of males kept for meat production are then put on different feeding programs appropriate for their commercial roles. Different segments of the poultry industry sex chickens for various reasons. In farms that produce eggs, males are unwanted; for meat production, separate male and female lines for breeding are maintained to produce the hybrid birds that are sold for the table, and chicks of the wrong sex in either line are unwanted. Chicks of an unwanted sex are killed almost immediately to reduce costs to the breeder. Meth ...
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Nuclear Sexing
Nuclear sexing is a technique for genetic sex determination in those species where XX chromosome pair is present. Nuclear sexing can be done by identifying Barr body, a drumstick like appendage located in the rim of the nucleus in somatic cells. Barr body is the inactive X chromosome which lies condensed in the nucleus of somatic cells. A typical human (or other XY-based organism) female has only one Barr body per somatic cell, while a typical human male has none. Though a Barr body can be sought in any human nucleated cell, circulating mononuclear cells are commonly used for this purpose. These cells are cultured, and treated with chemicals such as colcemid to arrest mitosis in metaphase. A minimum of 30 percent of sex chromatin indicates genetic female sex. See also * Sex-determination system A sex-determination system is a biological system that determines the development of sexual characteristics in an organism. Most organisms that create their offspring using sexual r ...
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Fiberscope
A fiberscope is a flexible optical fiber bundle with a lens on one end and an eyepiece or camera on the other. It is used to examine and inspect small, difficult-to-reach places such as the insides of machines, locks, and the human body. History Guiding of light by refraction, the principle that makes fiber optics possible, was first demonstrated by Daniel Colladon and Jacques Babinet in Paris in the early 1840s. Then in 1930, Heinrich Lamm, a German medical student, became the first person to put together a bundle of optical fibers to carry an image. These discoveries led to the invention of endoscopes and fiberscopes. In the 1960s the endoscope was upgraded with glass fiber, a flexible material that allowed light to transmit, even when bent. While this provided users with the capability of real-time observation, it did not provide them with the ability to take photographs. In 1964 the fiberscope, the first gastro camera, was invented. It was the first time an endoscope ...
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Restriction Enzyme
A restriction enzyme, restriction endonuclease, REase, ENase or'' restrictase '' is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes. Restriction enzymes are commonly classified into five types, which differ in their structure and whether they cut their DNA enzyme substrate (biology), substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. To cut DNA, all restriction enzymes make two incisions, once through each backbone chain, sugar-phosphate backbone (i.e. each strand) of the DNA double helix. These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses. Inside a prokaryote, the restriction enzymes selectively cut up ''foreign'' DNA in a process called ''restriction digestion''; meanwhile, host DNA is protected by a modification ...
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Sexual Dimorphism
Sexual dimorphism is the condition where sexes of the same species exhibit different Morphology (biology), morphological characteristics, including characteristics not directly involved in reproduction. The condition occurs in most dioecy, dioecious species, which consist of most animals and some plants. Differences may include secondary sex characteristics, size, weight, color, markings, or behavioral or cognitive traits. Male-male reproductive competition has evolved a diverse array of sexually dimorphic traits. Aggressive utility traits such as "battle" teeth and blunt heads reinforced as battering rams are used as weapons in aggressive interactions between rivals. Passive displays such as ornamental feathering or song-calling have also evolved mainly through sexual selection. These differences may be subtle or exaggerated and may be subjected to sexual selection and natural selection. The opposite of dimorphism is ''monomorphism'', when both biological sexes are phenotype, ...
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Polymerase Chain Reaction
The polymerase chain reaction (PCR) is a method widely used to make millions to billions of copies of a specific DNA sample rapidly, allowing scientists to amplify a very small sample of DNA (or a part of it) sufficiently to enable detailed study. PCR was invented in 1983 by American biochemist Kary Mullis at Cetus Corporation. Mullis and biochemist Michael Smith (chemist), Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993. PCR is fundamental to many of the procedures used in genetic testing and research, including analysis of Ancient DNA, ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications including biomedical research and forensic ...
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Amelogenin
Amelogenins are a group of protein isoforms produced by alternative splicing or proteolysis from the '' AMELX'' gene, on the X chromosome, and also the '' AMELY'' gene in males, on the Y chromosome. They are involved in amelogenesis, the development of enamel. Amelogenins are type of extracellular matrix protein, which, together with ameloblastins, enamelins and tuftelins, direct the mineralization of enamel to form a highly organized matrix of rods, interrod crystal and proteins. Although the precise role of amelogenin(s) in regulating the mineralization process is unknown, it is known that amelogenins are abundant during amelogenesis. Developing human enamel contains about 70% protein, 90% of which are amelogenins. Function Amelogenins are believed to be involved in the organizing of enamel rods during tooth development. The latest research indicates that these proteins regulate the initiation and growth of hydroxyapatite crystals during the mineralization of enamel. ...
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Parrots
Parrots (Psittaciformes), also known as psittacines (), are birds with a strong curved beak, upright stance, and clawed feet. They are classified in four families that contain roughly 410 species in 101 genus (biology), genera, found mostly in tropics, tropical and subtropics, subtropical regions. The four families are the Psittaculidae (Old World parrots), Psittacidae (African and New World parrots), Cacatuidae (cockatoos), and Strigopidae (New Zealand parrots). One-third of all parrot species are threatened by extinction, with a higher aggregate extinction risk (Red List Index, IUCN Red List Index) than any other comparable bird group. Parrots have a generally pantropical distribution with several species inhabiting temperateness, temperate regions as well. The greatest biodiversity, diversity of parrots is in South America and Australasia. Parrotsalong with Corvidae, ravens, crows, jays, and magpiesare among the most #Intelligence and learning, intelligent birds, and the abil ...
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General Anaesthesia
General anaesthesia (UK) or general anesthesia (US) is medically induced loss of consciousness that renders a patient unarousable even by painful stimuli. It is achieved through medications, which can be injected or inhaled, often with an analgesic and neuromuscular blocking agent. General anaesthesia is usually performed in an operating theatre to allow surgical procedures that would otherwise be intolerably painful for a patient, or in an intensive care unit or emergency department to facilitate endotracheal intubation and mechanical ventilation in critically ill patients. Depending on the procedure, general anaesthesia may be optional or required. No matter whether the patient prefers to be unconscious or not, certain pain stimuli can lead to involuntary responses from the patient, such as movement or muscle contractions, that make the operation extremely difficult. Thus, for many procedures, general anaesthesia is necessary from a practical point of view. The patient's n ...
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The Wilson Journal Of Ornithology
''The Wilson Journal of Ornithology'' (until 2006 ''The Wilson Bulletin'') is a quarterly peer-reviewed scientific journal published by the Wilson Ornithological Society. Both the society and its journal were named after American ornithologist Alexander Wilson. The journal consists of ornithological studies, short communications on bird observations, and book reviews. Most of the work disseminated through the journal is conducted in the Western Hemisphere. Abstracting and indexing The journal is abstracted and indexed in: * Science Citation Index Expanded * Scopus * Academic Search Ultimate * BIOSIS * GEOBASE * CAB Abstracts See also *List of ornithology journals The following is a list of notable journals and magazines relating to birding and ornithology. The continent and country columns give the location where the journal or magazine is published and may not correspond with its scope or content. See ... References External links * BioOne''The Wilson Bulletin'' Vol. ...
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Amphibians
Amphibians are ectothermic, anamniote, anamniotic, tetrapod, four-limbed vertebrate animals that constitute the class (biology), class Amphibia. In its broadest sense, it is a paraphyletic group encompassing all Tetrapod, tetrapods, but excluding the amniotes (tetrapods with an amniotic membrane, such as modern reptiles, birds and mammals). All extant taxon, extant (living) amphibians belong to the monophyletic subclass (biology), subclass Lissamphibia, with three living order (biology), orders: Anura (frogs and toads), Urodela (salamanders), and Gymnophiona (caecilians). Evolved to be mostly semiaquatic, amphibians have adapted to inhabit a wide variety of habitats, with most species living in freshwater ecosystem, freshwater, wetland or terrestrial ecosystems (such as riparian woodland, fossorial and even arboreal habitats). Their biological life cycle, life cycle typically starts out as aquatic animal, aquatic larvae with gills known as tadpoles, but some species have devel ...
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