Rosaniline Hydrochloride
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Rosaniline Hydrochloride
Fuchsine (sometimes spelled fuchsin) or rosaniline hydrochloride is a magenta dye with chemical formula C20H19N3·HCl."Basic chemical data"
''Discovery Series'' online database, Developmental Therapeutics Program, U.S. National Institutes of Health. Retrieved on 2007-10-08.
There are other similar chemical formulations of products sold as fuchsine, and several dozen other synonyms of this molecule. It becomes magenta when dissolved in ; as a , it forms dark

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Magenta
Magenta () is a purple-red color. On color wheels of the RGB color model, RGB (additive) and subtractive color, CMY (subtractive) color models, it is located precisely midway between blue and red. It is one of the four colors of ink used in color printing by most Color printing, color printers, along with yellow, cyan, and black to make all the other colors. Magenta is a color made by mixing red and blue. The tone of magenta used in printing, Shades of magenta, printer's magenta, is redder than the magenta of the RGB (additive) model, the former being closer to Rose (color), rose. Magenta took its name from an aniline dye made and patented in 1859 by the French chemist François-Emmanuel Verguin, who originally called it ''fuchsine''. It was renamed to celebrate the French-Sardinian victory under French Emperor Napoleon III at the Battle of Magenta against the larger army of the Austrian Empire on 4 June 1859 near the Italian town of Magenta, Lombardy, Magenta, at the time in Au ...
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Carbon Tetrachloride
Carbon tetrachloride, also known by many other names (such as carbon tet for short and tetrachloromethane, also IUPAC nomenclature of inorganic chemistry, recognised by the IUPAC), is a chemical compound with the chemical formula CCl4. It is a non-flammable, dense, colourless liquid with a "sweet" chloroform-like odour that can be detected at low levels. It was formerly widely used in fire extinguishers, as a precursor to refrigerants, an anthelmintic and a cleaning agent, but has since been phased out because of environmental and safety concerns. Exposure to high concentrations of carbon tetrachloride can affect the central nervous system and degenerate the liver and kidneys. Prolonged exposure can be fatal. Properties In the carbon tetrachloride molecule, four chlorine atoms are positioned symmetrically as corners in a tetrahedron, tetrahedral configuration joined to a central carbon atom by single covalent bonds. Because of this symmetric geometry, CCl4 is non-polar. methane, ...
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Ziehl–Neelsen Stain
The Ziehl-Neelsen stain, also known as the acid-fast stain, is a bacteriological staining technique used in cytopathology and microbiology to identify acid-fast bacteria under microscopy, particularly members of the ''Mycobacterium'' genus. This staining method was initially introduced by Paul Ehrlich (1854–1915) and subsequently modified by the German bacteriologists Franz Ziehl (1859–1926) and Friedrich Neelsen (1854–1898) during the late 19th century. The acid-fast staining method, in conjunction with auramine phenol staining, serves as the standard diagnostic tool and is widely accessible for rapidly diagnosing tuberculosis (caused by ''Mycobacterium tuberculosis'') and other diseases caused by atypical mycobacteria, such as leprosy (caused by ''Mycobacterium leprae'') and ''Mycobacterium avium-intracellulare'' infection (caused by ''Mycobacterium avium'' complex) in samples like sputum, gastric washing fluid, and bronchoalveolar lavage fluid. These acid-fast bact ...
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Carbol Fuchsin
Carbol fuchsin, carbol-fuchsin, carbolfuchsin, or Castellani's paint is a mixture of phenol and basic fuchsin that is used in bacterial staining procedures. It is commonly used in the staining of mycobacteria because it has an affinity for the mycolic acids found in their cell membranes. It is a component of Ziehl–Neelsen stain, a differential staining, differential stain. Carbol fuchsin is used as the primary stain dye to detect acid-fast, acid-fast bacteria because it is more soluble in the cells' wall lipids than in the acid alcohol. If the bacteria is acid-fast the bacteria will retain the initial red color of the dye because they are able to resist the destaining by acid alcohol (0.4–1% HCl in 70% EtOH). Additionally, it can be used for the staining of bacterial spores. Carbol-fuchsin is also used as a topical antiseptic and antifungal. References

{{Stains Microscopy Microbiology techniques Laboratory techniques Histopathology Histotechnology Staining dyes Staini ...
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Phenol
Phenol (also known as carbolic acid, phenolic acid, or benzenol) is an aromatic organic compound with the molecular formula . It is a white crystalline solid that is volatile and can catch fire. The molecule consists of a phenyl group () bonded to a hydroxy group (). Mildly acidic, it requires careful handling because it can cause chemical burns. It is acutely toxic and is considered a health hazard. Phenol was first extracted from coal tar, but today is produced on a large scale (about 7 million tonnes a year) from petroleum-derived feedstocks. It is an important industrial commodity as a precursor to many materials and useful compounds, and is a liquid when manufactured. It is primarily used to synthesize plastics and related materials. Phenol and its chemical derivatives are essential for production of polycarbonates, epoxies, explosives such as picric acid, Bakelite, nylon, detergents, herbicides such as phenoxy herbicides, and numerous pharmaceuti ...
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Schiff Reagent
: The Schiff test is an early organic chemistry named reaction developed by Hugo Schiff, and is a relatively general chemical test for detection of many organic aldehydes that has also found use in the staining of biological tissues. The Schiff reagent is the reaction product of a dye formulation such as fuchsin and sodium bisulfite; pararosaniline (which lacks an aromatic methyl group) and new fuchsin (which is uniformly mono-methylated ''ortho'' to the dye's amine functionalities) are not dye alternatives with comparable detection chemistry. In its use as a qualitative test for aldehydes, the unknown sample is added to the decolorized Schiff reagent; when aldehyde is present a characteristic magenta color develops. Schiff-type reagents are used for various biological tissue staining methods, e.g. Feulgen stain and periodic acid-Schiff stain. Human skin also contains aldehyde functional groups in the termini of saccharides and so is stained as well. Mechanism Fuchsin solutio ...
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New Fuchsine
New fuchsine is an organic compound with the formula H2N(CH3)C6H3)3Cl. It is a green-colored solid that is used as a dye of the triarylmethane class. It is one of the four components of basic fuchsine, and one of the two that are available as single dyes. The other is pararosaniline. It is prepared by condensation of ortho-toluidine with formaldehyde. This process initially gives the benzhydrol 4,4'-bis(dimethylamino)benzhydrol, which is further condensed to give the leuco (colorless) tertiary alcohol [(H2N(CH3)C6H3)3COH, which is oxidized in acid to give the dye. Use as dye and stain New fuchsine is used to dye polyacrylonitrile, paper, and leather. In biology, it can be used for staining (biology), staining acid-fast organisms, e.g. by Ziehl–Neelsen stain, and for making Schiff's reagent. As a primary amine, the dye can be diazotized in the laboratory, and the resulting diazonium salt used as a trapping agent in enzyme histochemistry.Lojda Z, Gossrau R, Schiebler TH (1 ...
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Pararosaniline
Pararosaniline, pararosaniline free base, Basic Red 9, or C.I. 42500 is an organic compound with the formula . It is the free base form of pararosaniline hydrochloride, , a magenta solid with a variety of uses as a dye. It is one of the four components of basic fuchsine. It is structurally related to other triarylmethane dyes called methyl violets (e.g. crystal violet) which feature methyl groups on nitrogen. It is prepared by the condensation of aniline and . Alternatively, it arises from the oxidation of 4,4'-bis(aminophenyl)methane in the presence of aniline. Uses *It is used to dye polyacrylonitrile fibers. *It is used to detect sulfur dioxide. *Pararosaniline is used as a colorimetric test for aldehydes, in the Schiff test. It is the only basic fuchsine component suitable for making the aldehyde-fuchsine stain for pancreatic islet beta cells. *It has use as an antischistosomal. Related compounds * 4,4'-Thiodianiline * 4,4'-Methylenedianiline * 4,4'-Oxydianiline * Dapso ...
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Sulfonic Acid
In organic chemistry, sulfonic acid (or sulphonic acid) refers to a member of the class of organosulfur compounds with the general formula , where R is an organic alkyl or aryl group and the group a sulfonyl hydroxide. As a substituent, it is known as a sulfo group. A sulfonic acid can be thought of as sulfuric acid with one hydroxyl group replaced by an organic substituent. The parent compound (with the organic substituent replaced by hydrogen) is the parent sulfonic acid, , a tautomer of sulfurous acid, . Salt (chemistry), Salts or esters of sulfonic acids are called sulfonates. Preparation Aryl sulfonic acids are produced by the process of sulfonation. Usually the sulfonating agent is sulfur trioxide. A large scale application of this method is the production of alkylbenzenesulfonic acids: : In this reaction, sulfur trioxide is an electrophile and the arene is the nucleophile. The reaction is an example of electrophilic aromatic substitution. In a related process, carboxyli ...
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Acid Fuchsine
Acid fuchsin or fuchsine acid, (also called Acid Violet 19 and C.I. 42685) is an acidic magenta dye with the chemical formula C20H17N3Na2O9S3. It is a sodium sulfonate derivative of fuchsine. Acid fuchsin has wide use in histology, and is one of the dyes used in Masson's trichrome stain. This method is commonly used to stain cytoplasm and nuclei of tissue sections in the histology laboratory in order to distinguish muscle from collagen. The muscle stains red with the acid fuchsin, and the collagen is stained green or blue with Light Green SF yellowish or methyl blue. It can also be used to identify growing bacteria. See also * New fuchsine * Pararosanilin * Verhoeff’s Stain Verhoeff's stain, also known as Verhoeff's elastic stain (VEG) or Verhoeff–Van Gieson stain (VVG), is a staining protocol used in histology, developed by American ophthalmic surgeon and pathologist Frederick Herman Verhoeff (1874–1968) in 190 ... * Pollen grain staining (Alexander's stain) Re ...
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Leonhart Fuchs
Leonhart Fuchs (; 17 January 1501 – 10 May 1566), sometimes spelled Leonhard Fuchs and cited in Latin as ''Leonhartus Fuchsius'', was a German physician and botanist. His chief notability is as the author of a large book about plants and their uses as medicines, a herbal, which was first published in 1542 in Latin. It has about 500 accurate and detailed drawings of plants, which were printed from woodcuts. The drawings are the book's most notable advance on its predecessors. Although drawings had been used in other herbal books, Fuchs's book proved and emphasized high-quality drawings as the most telling way to specify what a plant name stands for. Life Fuchs was born in 1501 in Wemding (Marktplatz 5), near Donauwörth in Donau-Ries in the then Duchy of Bavaria, as the youngest son of Johann (Hans) Fuchs and his wife Anna Denten. His father was the town Burgomaster, and both parents came from families of municipal councillors (''Ratsherr''). The exact date of his bir ...
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