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Rhodamine 6G
Rhodamine 6G is a highly fluorescent rhodamine family dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Forms Rhodamine 6G usually comes in three different forms. Rhodamine 6G chloride is a bronze/red powder with the chemical formula C28H31ClN2O3. Although highly soluble, this formulation is very corrosive to all metals except stainless steel. Other formulations are less soluble, but also less corrosive. Rhodamine 6G perchlorate (C28H31ClN2O7) comes in the form of red crystals, while rhodamine 6G tetrafluoroborate (C28H31BF4N2O3) appears as maroon crystals. Solubility Butanol (40 g/L), ethanol (80 g/L), methanol (400 g/L), propa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Methanol
Methanol (also called methyl alcohol and wood spirit, amongst other names) is an organic chemical and the simplest aliphatic alcohol, with the formula C H3 O H (a methyl group linked to a hydroxyl group, often abbreviated as MeOH). It is a light, volatile, colourless, flammable liquid with a distinctive alcoholic odour similar to that of ethanol (potable alcohol). A polar solvent, methanol acquired the name wood alcohol because it was once produced chiefly by the destructive distillation of wood. Today, methanol is mainly produced industrially by hydrogenation of carbon monoxide. Methanol consists of a methyl group linked to a polar hydroxyl group. With more than 20 million tons produced annually, it is used as a precursor to other commodity chemicals, including formaldehyde, acetic acid, methyl tert-butyl ether, methyl benzoate, anisole, peroxyacids, as well as a host of more specialised chemicals. Occurrence Small amounts of methanol are present in normal, h ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Isopropanol
Isopropyl alcohol (IUPAC name propan-2-ol and also called isopropanol or 2-propanol) is a colorless, flammable organic compound with a pungent alcoholic odor. As an isopropyl group linked to a hydroxyl group (chemical formula ) it is the simplest example of a secondary alcohol, where the alcohol carbon atom is attached to two other carbon atoms. It is a structural isomer of propan-1-ol and ethyl methyl ether. It is used in the manufacture of a wide variety of industrial and household chemicals and is a common ingredient in products such as antiseptics, disinfectants, hand sanitizer and detergents. Well over one million tonnes is produced worldwide annually. Properties Isopropyl alcohol is miscible in water, ethanol, and chloroform as, like these compounds, isopropyl is a polar molecule. It dissolves ethyl cellulose, polyvinyl butyral, many oils, alkaloids, and natural resins. Unlike ethanol or methanol, isopropyl alcohol is not miscible with salt solutions and ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Quantum Yield
The quantum yield (Φ) of a radiation-induced process is the number of times a specific event occurs per photon absorbed by the system. Applications Fluorescence spectroscopy The fluorescence quantum yield is defined as the ratio of the number of photons emitted to the number of photons absorbed.Lakowicz, Joseph R. ''Principles of Fluorescence Spectroscopy'' (Kluwer Academic / Plenum Publishers 1999) p.10. Fluorescence quantum yield is measured on a scale from 0 to 1.0, but is often represented as a percentage. A quantum yield of 1.0 (100%) describes a process where each photon absorbed results in a photon emitted. Substances with the largest quantum yields, such as rhodamines, display the brightest emissions; however, compounds with quantum yields of 0.10 are still considered quite fluorescent. Quantum yield is defined by the fraction of excited state fluorophores that decay through fluorescence: where \Phi_ is the fluorescence quantum yield, k_ is the rate constant ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Photobleaching
In optics, photobleaching (sometimes termed fading) is the photochemical alteration of a dye or a fluorophore molecule such that it is permanently unable to fluoresce. This is caused by cleaving of covalent bonds or non-specific reactions between the fluorophore and surrounding molecules. Such irreversible modifications in covalent bonds are caused by transition from a singlet state to the triplet state of the fluorophores. The number of excitation cycles to achieve full bleaching varies. In microscopy, photobleaching may complicate the observation of fluorescent molecules, since they will eventually be destroyed by the light exposure necessary to stimulate them into fluorescing. This is especially problematic in time-lapse microscopy. However, photobleaching may also be used prior to applying the (primarily antibody-linked) fluorescent molecules, in an attempt to quench autofluorescence. This can help improve the signal-to-noise ratio. Photobleaching may also be exploited to st ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Argon Ion Laser
An ion laser is a gas laser that uses an ionized gas as its lasing medium. Like other gas lasers, ion lasers feature a sealed cavity containing the laser medium and mirrors forming a Fabry–Pérot resonator. Unlike helium–neon lasers, the energy level transitions that contribute to laser action come from ions. Because of the large amount of energy required to excite the ionic transitions used in ion lasers, the required current is much greater, and as a result all but the smallest ion lasers are water-cooled. A small air-cooled ion laser might produce, for example, 130 milliwatts of output light with a tube current of about 10 amperes and a voltage of 105 volts. Since one ampere times one volt is one watt, this is an electrical power input of about one kilowatt. Subtracting the (desirable) light output of 130 mW from power input, this leaves the large amount of waste heat of nearly one kW. This has to be dissipated by the cooling system. In other words, the power efficiency i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nitrogen Laser
A nitrogen laser is a gas laser operating in the ultraviolet rangeC. S. Willett, ''Introduction to Gas Lasers: Population Inversion Mechanisms'' (Pergamon, New York,1974). (typically 337.1 nm) using molecular nitrogen as its gain medium, pumped by an electrical discharge. The wall-plug efficiency of the nitrogen laser is low, typically 0.1% or less, though nitrogen lasers with efficiency of up to 3% have been reported in the literature. The wall-plug efficiency is the product of the following three efficiencies: * electrical: TEA laser * gain medium: This is the same for all nitrogen lasers and thus has to be at least 3% ** inversion by electron impact is 10 to 1 due to Franck–Condon principle ** energy lost in the lower laser level: 40% * optical: More stimulated emission than spontaneous emission Gain medium The gain medium is nitrogen molecules in the gas phase. The nitrogen laser is a three-level laser. In contrast to more typical four-level lasers, the upper l ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dye Laser
A dye laser is a laser that uses an organic dye as the lasing medium, usually as a liquid solution. Compared to gases and most solid state lasing media, a dye can usually be used for a much wider range of wavelengths, often spanning 50 to 100 nanometers or more. The wide bandwidth makes them particularly suitable for tunable lasers and pulsed lasers. The dye rhodamine 6G, for example, can be tuned from 635 nm (orangish-red) to 560 nm (greenish-yellow), and produce pulses as short as 16 femtoseconds. Moreover, the dye can be replaced by another type in order to generate an even broader range of wavelengths with the same laser, from the near-infrared to the near-ultraviolet, although this usually requires replacing other optical components in the laser as well, such as dielectric mirrors or pump lasers. Dye lasers were independently discovered by P. P. Sorokin and F. P. Schäfer (and colleagues) in 1966. In addition to the usual liquid state, dye lasers are also avail ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gain Medium
The active laser medium (also called gain medium or lasing medium) is the source of optical gain within a laser. The gain results from the stimulated emission of photons through electronic or molecular transitions to a lower energy state from a higher energy state previously populated by a pump source. Examples of active laser media include: * Certain crystals, typically doped with rare-earth ions (e.g. neodymium, ytterbium, or erbium) or transition metal ions (titanium or chromium); most often yttrium aluminium garnet ( Y3 Al5 O12), yttrium orthovanadate (YVO4), or sapphire (Al2O3); and not often Caesium cadmium bromide ( Cs Cd Br3) (Solid-state lasers) * Glasses, e.g. silicate or phosphate glasses, doped with laser-active ions; * Gases, e.g. mixtures of helium and neon (HeNe), nitrogen, argon, krypton, carbon monoxide, carbon dioxide, or metal vapors; (Gas lasers) * Semiconductors, e.g. gallium arsenide (GaAs), indium gallium arsenide (InGaAs), or gallium nitride (GaN). * Li ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Laser Dye
Laser dyes are dyes used as laser medium in a dye laser. Laser dyes include the coumarins and the rhodamines. Coumarin dyes emit in the green region of the spectrum, whereas rhodamine dyes are used for emission in the yellow-red. The color emitted by the laser dyes depend upon the surrounding medium i.e.the medium in which they are dissolved. However, there are dozens of laser dyes that can be used to span continuously the emission spectrum from the near ultraviolet to the near infrared.F. J. Duarte, ''Tunable Laser Optics'' (Elsevier-Academic, New York, 2003) Appendix of Laser Dyes (includes more than 50 laser dyes) Laser dyes are also used to dope solid-state matrices, such as poly(methyl methacrylate) (PMMA), and ORMOSILs, to provide gain media for solid state dye lasers. Partial list of laser dyes *Coumarins (in various nomenclatures such as Coumarin 480, 490, 504, 521, 504T, 521T) *Fluorescein * polyphenyl ("polyphenyl 1") S. C. Guggenheimer, A. B. Petersen"High ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Coherent 899 Dye Laser
Coherence, coherency, or coherent may refer to the following: Physics * Coherence (physics), an ideal property of waves that enables stationary (i.e. temporally and spatially constant) interference * Coherence (units of measurement), a derived unit that, for a given system of quantities and for a chosen set of base units, is a product of powers of base units with no other proportionality factor than one * Coherence time, the time over which a propagating wave (especially a laser or maser beam) may be considered coherent; the time interval within which its phase is, on average, predictable Mathematics * Coherence (philosophical gambling strategy), a concept in Bayesian statistics * Coherence (signal processing), a statistic that can be used to examine the relation between two signals or data sets * Coherence (statistics), a property of self-consistency across a set of assessments, or the strength of association between two series * Coherence condition in category the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Polyethylene glycol
Polyethylene glycol (PEG; ) is a polyether compound derived from petroleum with many applications, from industrial manufacturing to medicine. PEG is also known as polyethylene oxide (PEO) or polyoxyethylene (POE), depending on its molecular weight. The structure of PEG is commonly expressed as H−(O−CH2−CH2)n−OH. Uses Medical uses * Pharmaceutical-grade PEG is used as an excipient in many pharmaceutical products, in oral, topical, and parenteral dosage forms. * PEG is the basis of a number of laxatives (as ''MiraLax, RestoraLAX, etc.''). Whole bowel irrigation with polyethylene glycol and added electrolytes is used for bowel preparation before surgery or colonoscopy or for children with constipation. Macrogol (with brand names such as Laxido, GoLytely and Miralax) is the generic name for polyethylene glycol used as a laxative. The name may be followed by a number which represents the average molecular weight (e.g. macrogol 3350, macrogol 4000 or macrogol 6000). ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
