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Ptk2 Cells
PtK2 cells are a cell line derived from male long-nosed potoroo (''Potorous tridactylis'') epithelial kidney cells. This cell line is used for a variety of applications in biomedical research but is particularly popular as a model for mitosis. Origin The PtK2 cell line was established by Kirsten Walen and Spencer Brown in 1962. Cells from the rat kangaroo were selected as the source of a cell line because this species has only a small number of chromosomes and these chromosomes are easily visualized under a microscope; mitosis becomes easy to observe, and the structural aspects of cell division can be closely examined. Characteristics PtK2 cells are relatively large, and when grown in a monolayer, stay flat throughout the cell cycle - unlike many cells that round up during mitosis. PtK2 cells are resistant to adenovirus 5, coxsackievirus B5, and poliovirus 2. They are susceptible to coxsackievirus A9, herpes simplex, vaccinia, and vesicular stomatitis (Ogden strain). PtK2 cells c ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cell Line
An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells can therefore be grown for prolonged periods ''in vitro''. The mutations required for immortality can occur naturally or be intentionally induced for experimental purposes. Immortal cell lines are a very important tool for research into the biochemistry and cell biology of multicellular organisms. Immortalised cell lines have also found uses in biotechnology. An immortalised cell line should not be confused with stem cells, which can also divide indefinitely, but form a normal part of the development of a multicellular organism. Relation to natural biology and pathology There are various immortal cell lines. Some of them are normal cell lines (e.g. derived from stem cells). Other immortalised cell lines are the ''in vitro'' equivalen ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Long-nosed Potoroo
The long-nosed potoroo (''Potorous tridactylus'') is a small, hopping, gerbil-like mammal native to forests and shrubland of southeastern Australia and Tasmania. A member of the rat-kangaroo family (Potoroidae), it lives alone and digs at night for fungi, roots, or small insects. It is also a marsupial (like kangaroos) and carries its young in a pouch. The long-nosed potoroo is threatened by habitat loss and introduced species such as cats or foxes. There are two subspecies: ''P. t. tridactylus'' on mainland Australia, and ''P. t. apicalis'' on Tasmania, with lighter fur. At first glance, the long-nosed potoroo with its pointed nose and grey-brown fur looks very much like a bandicoot — that is, until it hops away with its front feet tucked into its chest, revealing its close relationship with the kangaroo family. The long-nosed potoroo exhibits many morphological specializations such as an elongated pointed rostral region (nose), erect ears, large eyes, claws for digging, and l ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mitosis
In cell biology, mitosis () is a part of the cell cycle in which replicated chromosomes are separated into two new nuclei. Cell division by mitosis gives rise to genetically identical cells in which the total number of chromosomes is maintained. Therefore, mitosis is also known as equational division. In general, mitosis is preceded by S phase of interphase (during which DNA replication occurs) and is often followed by telophase and cytokinesis; which divides the cytoplasm, organelles and cell membrane of one cell into two new cells containing roughly equal shares of these cellular components. The different stages of mitosis altogether define the mitotic (M) phase of an animal cell cycle—the division of the mother cell into two daughter cells genetically identical to each other. The process of mitosis is divided into stages corresponding to the completion of one set of activities and the start of the next. These stages are preprophase (specific to plant cells), p ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Keratin
Keratin () is one of a family of structural fibrous proteins also known as ''scleroproteins''. Alpha-keratin (α-keratin) is a type of keratin found in vertebrates. It is the key structural material making up scales, hair, nails, feathers, horns, claws, hooves, and the outer layer of skin among vertebrates. Keratin also protects epithelial cells from damage or stress. Keratin is extremely insoluble in water and organic solvents. Keratin monomers assemble into bundles to form intermediate filaments, which are tough and form strong unmineralized epidermal appendages found in reptiles, birds, amphibians, and mammals. Excessive keratinization participate in fortification of certain tissues such as in horns of cattle and rhinos, and armadillos' osteoderm. The only other biological matter known to approximate the toughness of keratinized tissue is chitin. Keratin comes in two types, the primitive, softer forms found in all vertebrates and harder, derived forms found only ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
