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Physode
Phlorotannins are a type of tannins found in brown algae such as kelps and rockweeds or sargassacean species, and in a lower amount also in some red algae. Contrary to hydrolysable or condensed tannins, these compounds are oligomers of phloroglucinol (polyphloroglucinols). As they are called tannins, they have the ability to precipitate proteins. It has been noticed that some phlorotannins have the ability to oxidize and form covalent bonds with some proteins. In contrast, under similar experimental conditions three types of terrestrial tannins (procyanidins, profisetinidins, and gallotannins) apparently did not form covalent complexes with proteins. These phenolic compounds are integral structural components of cell walls in brown algae, but they also seem to play many other secondary ecological roles such as protection from UV radiation and defense against grazing. Biosynthesis and localization Most of the phlorotannins' biosynthesis is still unknown, but it appears they ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Tetrafucol A
Tetrafucol A is a fucol-type phlorotannin found in the brown algae ''Ascophyllum nodosum'', ''Analipus japonicus ''Analipus japonicus'', or sea fir, is a brown alga species in the genus ''Analipus''. This species contains the phlorotannins difucol, trifucol, tetrafucol A and B, two pentafucols, four hexafucols, a heptafucol mixture and halogenated com ...'' and '' Scytothamnus australis''. In ''A. japonicus'', 5'-bromo- and 5'-chlorotetrafucol-A can also be detected. References Phlorotannins Natural phenol tetramers {{aromatic-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Vanillin–HCl Staining
Vanillin–HCl staining (10% vanillin and 90% of a mixture of ethanol and HCl, giving an orange color) can be used to visualize the localisation of tannins in cells. The localization of phlorotannins can be investigated by light microscopy after vanillin–HCl staining.Cytological studies on physodes in the vegetative cells of Cystoseira stricter Sauvagea (Phaeophyta, Fucales). Pellegrini L, J. Cell Sci., 1980, volume 41, pages 209–231 The phlorotannins can be seen this way in physodes in brown algae. The vanillin-HCl method can be used to estimate the proanthocyanidins content in plant cells, resulting in a red color of the test in the presence of catechin Catechin is a flavan-3-ol, a type of secondary metabolite providing antioxidant roles in plants. It belongs to the subgroup of polyphenols called flavonoids. The name of the catechin chemical family derives from '' catechu'', which is the ta ... or proanthocyanidins. References Staining {{cell-biology-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Eckol
Eckol is a phlorotannin isolated from brown algae in the family Lessoniaceae such as species in the genus ''Ecklonia'' such as '' E. cava'' or '' E. kurome'' or in the genus '' Eisenia'' such as '' Eisenia bicyclis''. The molecule possesses a dibenzo-''p''-dioxin skeleton and a phloroglucinol component. The molecule can also be viewed as a phloroglucinol trimer. It exhibits an antiplasmin inhibitory effect and a radioprotective effect in a mouse model. It also exhibits an ''in vitro'' cytoprotective effect against oxidative stress in Chinese hamster lung fibroblasts. It also shows antithrombotic An antithrombotic agent is a drug that reduces the formation of blood clots ( thrombi).http://cancerweb.ncl.ac.uk/cgi-bin/omd?antithrombotic Antithrombotics can be used therapeutically for prevention ( primary prevention, secondary prevention) or ... and profibrinolytic activities. References {{phlorotannin Phlorotannins Dibenzodioxins ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Prussian Blue Assay
Prussian blue (also known as Berlin blue, Brandenburg blue or, in painting, Parisian or Paris blue) is a dark blue pigment produced by oxidation of ferrous ferrocyanide salts. It has the chemical formula Fe Cyanide.html" ;"title="e(Cyanide">CN) Turnbull's blue is chemically identical, but is made from different reagents, and its slightly different color stems from different impurities and particle sizes. Prussian blue was the first modern synthetic pigment. It is prepared as a very fine colloidal dispersion, because the compound is not soluble in water. It contains variable amounts of other ions and its appearance depends sensitively on the size of the colloidal particles. The pigment is used in paints, and it is the traditional "blue" in blueprints, and became prominent in 19th-century () Japanese woodblock prints. In medicine, orally administered Prussian blue is used as an antidote for certain kinds of heavy metal poisoning, e.g., by thallium(I) and radioactive isotopes ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ascorbic Acid
Vitamin C (also known as ascorbic acid and ascorbate) is a water-soluble vitamin found in citrus and other fruits and vegetables, also sold as a dietary supplement and as a topical 'serum' ingredient to treat melasma (dark pigment spots) and wrinkles on the face. It is used to prevent and treat scurvy. Vitamin C is an essential nutrient involved in the repair of tissue, the formation of collagen, and the enzymatic production of certain neurotransmitters. It is required for the functioning of several enzymes and is important for immune system function. It also functions as an antioxidant. Most animals are able to synthesize their own vitamin C. However, apes (including humans) and monkeys (but not all primates), most bats, some rodents, and certain other animals must acquire it from dietary sources. There is some evidence that regular use of supplements may reduce the duration of the common cold, but it does not appear to prevent infection. It is unclear whether s ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pyridine
Pyridine is a basic (chemistry), basic heterocyclic compound, heterocyclic organic compound with the chemical formula . It is structurally related to benzene, with one methine group replaced by a nitrogen atom. It is a highly flammable, weakly alkaline, water-miscible liquid with a distinctive, unpleasant fish-like smell. Pyridine is colorless, but older or impure samples can appear yellow, due to the formation of extended, unsaturated polymeric chains, which show significant electrical conductivity. The pyridine ring occurs in many important compounds, including agrochemicals, pharmaceuticals, and vitamins. Historically, pyridine was produced from coal tar. As of 2016, it is synthesized on the scale of about 20,000 tons per year worldwide. Properties Physical properties The molecular electric dipole moment is 2.2 debyes. Pyridine is diamagnetism, diamagnetic and has a Magnetic susceptibility, diamagnetic susceptibility of −48.7 × 10−6 cm3·mol−1. Th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Acetic Anhydride
Acetic anhydride, or ethanoic anhydride, is the chemical compound with the formula (CH3CO)2O. Commonly abbreviated Ac2O, it is the simplest isolable anhydride of a carboxylic acid and is widely used as a reagent in organic synthesis. It is a colorless liquid that smells strongly of acetic acid, which is formed by its reaction with moisture in the air. Structure and properties Acetic anhydride, like most acid anhydrides, is a flexible molecule with a nonplanar structure. The pi system linkage through the central oxygen offers very weak resonance stabilization compared to the dipole-dipole repulsion between the two carbonyl oxygens. The energy barriers to bond rotation between each of the optimal aplanar conformations are quite low. Like most acid anhydrides, the carbonyl carbon atom of acetic anhydride has electrophilic character, as the leaving group is carboxylate. The internal asymmetry may contribute to acetic anhydride's potent electrophilicity as the asymmetric geomet ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Extraction (chemistry)
Extraction in chemistry is a separation process consisting of the separation of a substance from a matrix. Common examples include '' liquid-liquid extraction'', and '' solid phase extraction''. The distribution of a solute between two phases is an equilibrium condition described by partition theory. This is based on exactly how the analyte moves from the initial solvent into the extracting solvent. The term ''washing'' may also be used to refer to an extraction in which impurities are extracted from the solvent containing the desired compound. Types of extraction * Liquid–liquid extraction * Solid-phase extraction * Acid-base extraction * Supercritical fluid extraction * Ultrasound-assisted extraction * Heat reflux extraction * Mechanochemical-assisted extraction * Maceration * Microwave-assisted extraction * Instant controlled pressure drop extraction (DIC, from the French, Détente instantanée contrôlée) * Perstraction Laboratory applications and examples L ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Lead Citrate
Lead citrate is a compound of lead and citrate that is primarily used as an enhancer for heavy metal staining in electron microscopy. It binds to osmium and uranyl acetate Uranyl acetate is the acetate salt of uranium oxide, a toxic yellow-green powder useful in certain laboratory tests. Structurally, it is a coordination polymer with formula UO2(CH3CO2)2(H2O)·H2O. Structure In the polymer, uranyl (UO22+) ... and enhances contrast in many cellular structures. Lead citrate is highly reactive with carbon dioxide. References {{reflist Lead compounds Citrates ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Uranyl Acetate
Uranyl acetate is the acetate salt of uranium oxide, a toxic yellow-green powder useful in certain laboratory tests. Structurally, it is a coordination polymer with formula UO2(CH3CO2)2(H2O)·H2O. Structure In the polymer, uranyl (UO22+) centers are bridged by acetate ligands. The remainder of each (heptacoordinate) coordination sphere is provided by an aquo ligand and a bidentate acetate ligand. One water of crystallization occupies the lattice. Uses Uranyl acetate is extensively used as a negative stain in electron microscopy."Negative Staining" University of Oxford Most procedures in electron microscopy for biology require the use of uranyl acetate. Negative staining protocols typically treat the sample with 1% to 5% aqueous solution. Uranyl acetate staining is simple and q ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
