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Natronococcus Amylolyticus
In taxonomy, ''Natronococcus'' is a genus of the Natrialbaceae. Description and significance This haloalkaliphilic archaeon is in the same family as microorganisms like ''Halobacterium''. Study of ''Natronococcus'' continues to explore what enzymes are present in order to survive in these conditions, especially since there has not been much literature about enzymes of haloalkaliphiles. Genome structure The G + C content for the major chromosome is 64.0% while the minor component has a 55.7% content. The approximate size of the plasmid is 144 kbp. Cell structure and metabolism ''Natronococcus'' is a heterotrophic, aerobic organism that can use sugars as an energy source to stimulate growth. It can fix nitrogen from casamino acids and reduces nitrates to nitrites. Cells are non- motile and occur in irregular clusters, pairs, and single cells. The cell is coccoid Coccoid means shaped like or resembling a coccus, that is, spherical.The noun coccoid or coccoids may re ...
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Archaea
Archaea ( ; singular archaeon ) is a domain of single-celled organisms. These microorganisms lack cell nuclei and are therefore prokaryotes. Archaea were initially classified as bacteria, receiving the name archaebacteria (in the Archaebacteria kingdom), but this term has fallen out of use. Archaeal cells have unique properties separating them from the other two domains, Bacteria and Eukaryota. Archaea are further divided into multiple recognized phyla. Classification is difficult because most have not been isolated in a laboratory and have been detected only by their gene sequences in environmental samples. Archaea and bacteria are generally similar in size and shape, although a few archaea have very different shapes, such as the flat, square cells of '' Haloquadratum walsbyi''. Despite this morphological similarity to bacteria, archaea possess genes and several metabolic pathways that are more closely related to those of eukaryotes, notably for the enzymes invo ...
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Microorganisms
A microorganism, or microbe,, ''mikros'', "small") and ''organism'' from the el, ὀργανισμός, ''organismós'', "organism"). It is usually written as a single word but is sometimes hyphenated (''micro-organism''), especially in older texts. The informal synonym ''microbe'' () comes from μικρός, mikrós, "small" and βίος, bíos, "life". is an organism of microscopic size, which may exist in its single-celled form or as a colony of cells. The possible existence of unseen microbial life was suspected from ancient times, such as in Jain scriptures from sixth century BC India. The scientific study of microorganisms began with their observation under the microscope in the 1670s by Anton van Leeuwenhoek. In the 1850s, Louis Pasteur found that microorganisms caused food spoilage, debunking the theory of spontaneous generation. In the 1880s, Robert Koch discovered that microorganisms caused the diseases tuberculosis, cholera, diphtheria, and anthrax. Because ...
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NaCl
Sodium chloride , commonly known as salt (although sea salt also contains other chemical salts), is an ionic compound with the chemical formula NaCl, representing a 1:1 ratio of sodium and chloride ions. With molar masses of 22.99 and 35.45 g/mol respectively, 100 g of NaCl contains 39.34 g Na and 60.66 g Cl. Sodium chloride is the salt most responsible for the salinity of seawater and of the extracellular fluid of many multicellular organisms. In its edible form, salt (also known as '' table salt'') is commonly used as a condiment and food preservative. Large quantities of sodium chloride are used in many industrial processes, and it is a major source of sodium and chlorine compounds used as feedstocks for further chemical syntheses. Another major application of sodium chloride is de-icing of roadways in sub-freezing weather. Uses In addition to the familiar domestic uses of salt, more dominant applications of the approximately 250 million tonnes per year production (2008 d ...
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Lake Magadi
Lake Magadi is the southernmost lake in the Kenyan Rift Valley, lying in a catchment of faulted volcanic rocks, north of Tanzania's Lake Natron. During the dry season, it is 80% covered by soda and is well known for its wading birds, including flamingos. Lake Magadi is a saline, alkaline lake, approximately 100 square kilometers in size, that lies in an endorheic basin formed by a graben. The lake is an example of a "saline pan". The lake water, which is a dense sodium carbonate brine, precipitates vast quantities of the mineral trona (sodium sesquicarbonate). In places, the salt is up to 40 m thick. The lake is recharged mainly by saline hot springs (temperatures up to 86 °C) that discharge into alkaline "lagoons" around the lake margins, there being little surface runoff in this arid region. Most hot springs lie along the northwestern and southern shorelines of the lake. During the rainy season, a thin (less than 1 m) layer of brine covers much of the saline pan, bu ...
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Coccus
A coccus (plural cocci) is any bacterium or archaeon that has a spherical, ovoid, or generally round shape. Bacteria are categorized based on their shapes into three classes: cocci (spherical-shaped), bacillus (rod-shaped) and spiral ( of which there are two types: spirillum and spirochete). Coccus refers to the shape of the bacteria, and can contain multiple genera, such as staphylococci or streptococci. Cocci can grow in pairs, chains, or clusters, depending on their orientation and attachment during cell division. In contrast to many bacilli-shaped bacteria, most cocci bacteria do not have flagella and are non-motile. Cocci is an English loanword of a modern or neo-Latin noun, which in turn stems from the Greek masculine noun () meaning 'berry'. Structure The cell wall structure for cocci may vary between gram-positive (thick peptidoglycan layers) and gram-negative (thin peptidoglycan layers). While living in their host organism, cocci can be pathogenic (e.g., strept ...
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Motility
Motility is the ability of an organism to move independently, using metabolic energy. Definitions Motility, the ability of an organism to move independently, using metabolic energy, can be contrasted with sessility, the state of organisms that do not possess a means of self-locomotion and are normally immobile. Motility differs from mobility, the ability of an object to be moved. The term vagility encompasses both motility and mobility; sessile organisms including plants and fungi often have vagile parts such as fruits, seeds, or spores which may be dispersed by other agents such as wind, water, or other organisms. Motility is genetically determined, but may be affected by environmental factors such as toxins. The nervous system and musculoskeletal system provide the majority of mammalian motility. In addition to animal locomotion, most animals are motile, though some are vagile, described as having passive locomotion. Many bacteria and other microorganisms, and multice ...
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Nitrites
The nitrite ion has the chemical formula . Nitrite (mostly sodium nitrite) is widely used throughout chemical and pharmaceutical industries. The nitrite anion is a pervasive intermediate in the nitrogen cycle in nature. The name nitrite also refers to organic compounds having the –ONO group, which are esters of nitrous acid. Production Sodium nitrite is made industrially by passing a mixture of nitrogen oxides into aqueous sodium hydroxide or sodium carbonate solution: : The product is purified by recrystallization. Alkali metal nitrites are thermally stable up to and beyond their melting point (441 °C for KNO2). Ammonium nitrite can be made from dinitrogen trioxide, N2O3, which is formally the anhydride of nitrous acid: :2 NH3 + H2O + N2O3 → 2 NH4NO2 Structure The nitrite ion has a symmetrical structure (C2v symmetry), with both N–O bonds having equal length and a bond angle of about 115°. In valence bond theory, it is described as a resonance hybrid with e ...
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Nitrates
Nitrate is a polyatomic ion with the chemical formula . Salts containing this ion are called nitrates. Nitrates are common components of fertilizers and explosives. Almost all inorganic nitrates are soluble in water. An example of an insoluble nitrate is bismuth oxynitrate. Structure The ion is the conjugate base of nitric acid, consisting of one central nitrogen atom surrounded by three identically bonded oxygen atoms in a trigonal planar arrangement. The nitrate ion carries a formal charge of −1. This charge results from a combination formal charge in which each of the three oxygens carries a − charge, whereas the nitrogen carries a +1 charge, all these adding up to formal charge of the polyatomic nitrate ion. This arrangement is commonly used as an example of resonance. Like the isoelectronic carbonate ion, the nitrate ion can be represented by resonance structures: Dietary nitrate A rich source of inorganic nitrate in the human diets come from leafy gre ...
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Casamino Acids
Casamino acids is a mixture of amino acids and some very small peptides obtained from acid hydrolysis of casein. It is typically used in microbial growth media. It has all the essential amino acids except tryptophan, which is destroyed by digestion with sulfuric or hydrochloric acid. Casamino acids is similar to tryptone, the latter differing by being an incomplete enzymatic hydrolysis with some oligopeptides present, while casamino acids is predominantly free amino acids. Uses Casamino acids supplies a completely hydrolyzed protein nitrogen source. It contains a small amount of cystine. Tryptophan and vitamins are destroyed by the acid treatment. The remaining amino acids (in varying amounts) are a source of nutrients for various microorganisms. Amino acids are highly soluble and suitable for use in tissue culture. Salt content is typically 30-40%. Casamino acids are either found in the Daptacel brand DTaP vaccine or used in its manufacture. Appearance *White to light tan, homog ...
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Aerobic Organism
Aerobic means "requiring air," in which "air" usually means oxygen. Aerobic may also refer to * Aerobic exercise, prolonged exercise of moderate intensity * Aerobics, a form of aerobic exercise * Aerobic respiration, the aerobic process of cellular respiration * Aerobic organism, a living thing with an oxygen-based metabolism See also * Anaerobic (other) Anaerobic means "living, active, occurring, or existing in the absence of free oxygen", as opposed to aerobic which means "living, active, or occurring only in the presence of oxygen." Anaerobic may also refer to: * Anaerobic adhesive, a bonding a ...
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Heterotrophic
A heterotroph (; ) is an organism that cannot produce its own food, instead taking nutrition from other sources of organic carbon, mainly plant or animal matter. In the food chain, heterotrophs are primary, secondary and tertiary consumers, but not producers. Living organisms that are heterotrophic include all animals and fungi, some bacteria and protists, and many parasitic plants. The term heterotroph arose in microbiology in 1946 as part of a classification of microorganisms based on their type of nutrition. The term is now used in many fields, such as ecology in describing the food chain. Heterotrophs may be subdivided according to their energy source. If the heterotroph uses chemical energy, it is a chemoheterotroph (e.g., humans and mushrooms). If it uses light for energy, then it is a photoheterotroph (e.g., green non-sulfur bacteria). Heterotrophs represent one of the two mechanisms of nutrition ( trophic levels), the other being autotrophs (''auto'' = self, ''tr ...
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Plasmid
A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that benefit the survival of the organism and confer selective advantage such as antibiotic resistance. While chromosomes are large and contain all the essential genetic information for living under normal conditions, plasmids are usually very small and contain only additional genes that may be useful in certain situations or conditions. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms. In the laboratory, plasmids may be introduced into a cell via transformation. Synthetic plasmids are available for procurement over the in ...
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