Microinject
Microinjection is the use of a glass micropipette to inject a liquid substance at a microscopic or borderline macroscopic level. The target is often a living cell but may also include intercellular space. Microinjection is a simple mechanical process usually involving an inverted microscope with a Optical power, magnification power of around 200x (though sometimes it is performed using a dissecting stereo microscope at 40–50x or a traditional Optical microscope#Compound microscope, compound upright microscope at similar power to an inverted model). For processes such as cellular or pronucleus, pronuclear injection the target cell is positioned under the microscope and two micromanipulation, micromanipulators—one holding the pipette and one holding a microcapillary needle usually between 0.5 and 5 μm in diameter (larger if injecting stem cells into an embryo)—are used to penetrate the cell membrane and/or the nuclear envelope. In this way the process can be used to int ...
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