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Methanogens In Digestive Tract Of Ruminants
Methanogens are a group of microorganisms that produce methane as a byproduct of their metabolism. They play an important role in the digestive system of ruminants. The digestive tract of ruminants contains four major parts: rumen, reticulum, omasum and abomasum. The food with saliva first passes to the rumen for breaking into smaller particles and then moves to the reticulum, where the food is broken into further smaller particles. Any indigestible particles are sent back to the rumen for rechewing. The majority of anaerobic microbes assisting the cellulose breakdown occupy the rumen and initiate the fermentation process. The animal absorbs the fatty acids, vitamins and nutrient content on passing the partially digested food from the rumen to the omasum. This decreases the pH level and initiates the release of enzymes for further breakdown of the food which later passes to the abomasum to absorb remaining nutrients before excretion. This process takes about 9–12 hours. Some of t ...
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Methanogens
Methanogens are anaerobic archaea that produce methane as a byproduct of their energy metabolism, i.e., catabolism. Methane production, or methanogenesis, is the only biochemical pathway for ATP generation in methanogens. All known methanogens belong exclusively to the domain Archaea, although some bacteria, plants, and animal cells are also known to produce methane. However, the biochemical pathway for methane production in these organisms differs from that in methanogens and does not contribute to ATP formation. Methanogens belong to various phyla within the domain Archaea. Previous studies placed all known methanogens into the superphylum Euryarchaeota. However, recent phylogenomic data have led to their reclassification into several different phyla. Methanogens are common in various anoxic environments, such as marine and freshwater sediments, wetlands, the digestive tracts of animals, wastewater treatment plants, rice paddy soil, and landfills. While some methanogens are ex ...
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Butyrivibrio Fibrisolvens
''Butyrivibrio'' is a genus of bacteria in Class Clostridia. Bacteria of this genus are common in the gastrointestinal systems of many animals. Genus ''Butyrivibrio'' was first described by Bryant and Small (1956) as anaerobic, butyric acid-producing, curved rods (or vibroids). ''Butyrivibrio'' cells are small, typically 0.4 – 0.6 μm by 2 – 5 μm. They are motile, using a single polar or subpolar monotrichous flagellum. They are commonly found singly or in short chains but it is not unusual for them to form long chains. Despite historically being described as Gram-negative, their cell walls contain derivatives of teichoic acid, and electron microscopy indicates that bacteria of this genus have a Gram-positive cell wall type.Beveridge, 1990 It is thought that they appear Gram-negative when Gram stained because their cell walls thin to 12 to 18 nm as they reach stationary phase. ''Butyrivibrio'' species are common in the rumens of ruminant animals such as cows, d ...
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Selenomonas Ruminantium
''Selenomonas ruminantium'' is a species of ''Selenomonas'' bacteria which are closely associated with ruminants, aiding in digestion of their food. It is predominantly observed in the rumen of these animals, and is strictly anaerobic. Shape and classification The bacterium has a rod-shaped structure. Gram classification 16S rRNA gene identification ''Selenomonas ruminantium'' was originally placed with the Gram-positive Firmicutes phylum based on its 16S rRNA gene. It was believed to be correct until the flagellar basal test was performed. Flagellar basal test The observation of flagella under electron microscope revealed the presence of four rings, a typical sign of gram negative species. Observation of the flagellar basal structure confirms that ''S. ruminantium'' has an outer membrane, a characteristic of Gram-negative bacteria . Gram-positive outer membranes or mycomembranes were recently reported for the Gram-positive general ''Corynebacterium'' and ''Mycobacter ...
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Succinate
Succinic acid () is a dicarboxylic acid with the chemical formula (CH2)2(CO2H)2. In living organisms, succinic acid takes the form of an anion, succinate, which has multiple biological roles as a metabolic intermediate being converted into Fumaric acid, fumarate by the enzyme succinate dehydrogenase in complex 2 of the electron transport chain which is involved in making Adenosine triphosphate, ATP, and as a signaling molecule reflecting the cellular metabolic state. Succinate is generated in mitochondria via the citric acid cycle, tricarboxylic acid (TCA) cycle. Succinate can exit the mitochondrial matrix and function in the cytoplasm as well as the extracellular space, changing gene expression patterns, modulating epigenetic landscape or demonstrating hormone-like signaling. As such, succinate links cellular metabolism, especially ATP formation, to the regulation of cellular function. Dysregulation of succinate synthesis, and therefore ATP synthesis, happens in some genetic m ...
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Acetate
An acetate is a salt formed by the combination of acetic acid with a base (e.g. alkaline, earthy, metallic, nonmetallic, or radical base). "Acetate" also describes the conjugate base or ion (specifically, the negatively charged ion called an anion) typically found in aqueous solution and written with the chemical formula . The neutral molecules formed by the combination of the acetate ion and a ''positive'' ion (called a cation) are also commonly called "acetates" (hence, ''acetate of lead'', ''acetate of aluminium'', etc.). The simplest of these is hydrogen acetate (called acetic acid) with corresponding salts, esters, and the polyatomic anion , or . Most of the approximately 5 million tonnes of acetic acid produced annually in industry are used in the production of acetates, which usually take the form of polymers. In nature, acetate is the most common building block for biosynthesis. Nomenclature and common formula When part of a salt, the formula of the acetate i ...
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Prevotella Ruminocola
''Prevotella'' is a genus of Gram-negative bacteria. ''Prevotella'' species are widely distributed across varied ecological habitats, with 57 characterized species spanning both human and other mammalian hosts. In mammals, this genus is notably prevalent in the swine gut microbiome. In humans, ''Prevotella'' species are members of the oral, vaginal, and gut microbiota and are often recovered from anaerobic infections of the respiratory tract. These infections include aspiration pneumonia, lung abscess, pulmonary empyema, and chronic otitis media and sinusitis. They have been isolated from abscesses and burns in the vicinity of the mouth, bites, paronychia, urinary tract infection, brain abscesses, osteomyelitis, and bacteremia associated with upper respiratory tract infections. ''Prevotella'' spp. predominate in periodontal disease and periodontal abscesses. Role in gut microbiota The human gut is mainly inhabited by two phyla of bacteria—''Bacillota'' and ''Bacteroidota ...
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Formate
Formate (IUPAC name: methanoate) is the conjugate base of formic acid. Formate is an anion () or its derivatives such as ester of formic acid. The salts and esters are generally colorless. Fundamentals When dissolved in water, formic acid converts to formate: : Formate is a planar anion. The two oxygen atoms are equivalent and bear a partial negative charge. The remaining C-H bond is not acidic. Biochemistry : Formate is a common C-1 source in living systems. It is formed from many precursors including choline, serine, and sarcosine. It provides a C-1 source in the biosynthesis of some nucleic acids. Formate (or formic acid) is invoked as a leaving group in the demethylation of some sterols. These conversions are catalyzed by aromatase enzymes using O2 as the oxidant. Specific conversions include testosterone to estradiol and androstenedione to estrone. Formate is reversibly oxidized by the enzyme formate dehydrogenase from ''Desulfovibrio gigas'': : Formate esters ...
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Propionate
Propionic acid (, from the Greek words πρῶτος : ''prōtos'', meaning "first", and πίων : ''píōn'', meaning "fat"; also known as propanoic acid) is a naturally occurring carboxylic acid with chemical formula . It is a liquid with a pungent and unpleasant smell somewhat resembling body odor. The anion as well as the salts and esters of propionic acid are known as propionates or propanoates. About half of the world production of propionic acid is consumed as a preservative for both animal feed and food for human consumption. It is also useful as an intermediate in the production of other chemicals, especially polymers. History Propionic acid was first described in 1844 by Johann Gottlieb, who found it among the degradation products of sugar. Over the next few years, other chemists produced propionic acid by different means, none of them realizing they were producing the same substance. In 1847, French chemist Jean-Baptiste Dumas established all the acids to be the sa ...
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