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Hemoglobinometer
A hemoglobinometer or haemoglobinometer (British English) is a medical device used to measure hemoglobin concentration in blood. It can operate by spectrophotometric measurement of hemoglobin concentration. Portable hemoglobinometers provide easy and convenient measurement of hematological variables, especially in areas where clinic laboratories are unavailable. As per guidelines of National AIDS Control Organisation (NACO) for accurate results & mass screening, analysis using hemoglobinometer is a recommended method used for absorbance measurement of whole blood at Hb/HbO2/ Isobestic point, based on microcuvette technology such as HemoCue 301 and Mokshit-Chanda-AM005A. Devices File:Haemoglobinometer (hemoglobinometer), United Kingdom, 1850-1950.jpg, Hemoglobinometry that compares a blood sample to a color chart, in use circa 1850 - 1950 File:American Optical Company hemoglobinometer.jpg, A hemoglobinometer made by the American Optical Company. The light of a battery-dri ...
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Hemoglobin
Hemoglobin (haemoglobin, Hb or Hgb) is a protein containing iron that facilitates the transportation of oxygen in red blood cells. Almost all vertebrates contain hemoglobin, with the sole exception of the fish family Channichthyidae. Hemoglobin in the blood carries oxygen from the respiratory organs (lungs or gills) to the other tissues of the body, where it releases the oxygen to enable aerobic respiration which powers an animal's metabolism. A healthy human has 12to 20grams of hemoglobin in every 100mL of blood. Hemoglobin is a metalloprotein, a chromoprotein, and a globulin. In mammals, hemoglobin makes up about 96% of a red blood cell's dry matter, dry weight (excluding water), and around 35% of the total weight (including water). Hemoglobin has an oxygen-binding capacity of 1.34mL of O2 per gram, which increases the total blood oxygen capacity seventy-fold compared to dissolved oxygen in blood plasma alone. The mammalian hemoglobin molecule can bind and transport up to four ...
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Haematology
Hematology ( spelled haematology in British English) is the branch of medicine concerned with the study of the cause, prognosis, treatment, and prevention of diseases related to blood. It involves treating diseases that affect the production of blood and its components, such as blood cells, hemoglobin, blood proteins, bone marrow, platelets, blood vessels, spleen, and the mechanism of coagulation. Such diseases might include hemophilia, sickle cell anemia, blood clots (thrombus), other bleeding disorders, and blood cancers such as leukemia, multiple myeloma, and lymphoma. The laboratory analysis of blood is frequently performed by a medical technologist or medical laboratory scientist. Specialization Physicians specialized in hematology are known as hematologists or haematologists. Their routine work mainly includes the care and treatment of patients with hematological diseases, although some may also work at the hematology laboratory viewing blood films and bone marrow s ...
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Absorbance
Absorbance is defined as "the logarithm of the ratio of incident to transmitted radiant power through a sample (excluding the effects on cell walls)". Alternatively, for samples which scatter light, absorbance may be defined as "the negative logarithm of one minus absorptance, as measured on a uniform sample". The term is used in many technical areas to quantify the results of an experimental measurement. While the term has its origin in quantifying the absorption of light, it is often entangled with quantification of light which is "lost" to a detector system through other mechanisms. What these uses of the term tend to have in common is that they refer to a logarithm of the ratio of a quantity of light incident on a sample or material to that which is detected after the light has interacted with the sample. The term absorption refers to the physical process of absorbing light, while absorbance does not always measure only absorption; it may measure attenuation (of transmitted ...
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Glucose Meter
A glucose meter, also referred to as a "glucometer", is a medical device for determining the approximate concentration of glucose in the blood. It can also be a strip of glucose paper dipped into a substance and measured to the glucose chart. It is a key element of glucose testing, including home blood glucose monitoring (HBGM) performed by people with diabetes mellitus or hypoglycemia. A small drop of blood, obtained from slightly piercing a fingertip with a Blood lancet, lancet, is placed on a disposable test strip that the meter reads and uses to calculate the blood glucose level. The meter then displays the level in units of mg/dL or mmol/L. Since approximately 1980, a primary goal of the management of diabetes mellitus type 1, type 1 diabetes and type 2 diabetes mellitus has been achieving closer-to-normal levels of glucose in the blood for as much of the time as possible, guided by HBGM several times a day. The benefits include a reduction in the occurrence rate and sev ...
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Cytometry
Cytometry is the measurement of number and characteristics of cell (biology), cells. Variables that can be measured by cytometric methods include cell size, cell counting, cell count, cell morphology (shape and structure), cell cycle phase, DNA content, and the existence or absence of specific proteins on the cell surface or in the cytoplasm. Cytometry is used to characterize and count blood cells in common blood tests such as the complete blood count. In a similar fashion, cytometry is also used in cell biology research and in medical diagnostics to characterize cells in a wide range of applications associated with diseases such as cancer and AIDS. Cytometric devices Image cytometers Image cytometry is the oldest form of cytometry. Image cytometers operate by statically imaging a large number of cells using optical microscopy. Prior to analysis, cells are commonly stained to enhance contrast or to detect specific molecules by labeling these with fluorochromes. Traditionally, cel ...
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Hemocytometer
The hemocytometer (or haemocytometer, or Burker's chamber) is a counting-chamber device originally designed and usually used for counting blood cells. The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a rectangular indentation that creates a precision volume chamber. This chamber is engraved with a laser-etched grid of perpendicular lines. The device is carefully crafted so that the area bounded by the lines is known, and the depth of the chamber is also known. By observing a defined area of the grid, it is therefore possible to count the number of cells or particles in a specific volume of fluid, and thereby calculate the concentration of cells in the fluid overall. A well used type of hemocytometer is the ''Neubauer'' counting chamber. Other types of hemocytometers with different rulings are in use for different applications. Fuchs-Rosenthal rulings, commonly used for spinal fluid counting, Howard Mold rulings us ...
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American Optical Company
The American Optical Company, also known as AO Eyewear, is a luxury American eyewear and sunglass company based in Vernon Hills, Illinois near Chicago. AO designs and manufactures in the United States. History Founded in 1833 by William Beecher, AO was later bought by fellow apprentice Robert H. Cole who became the largest shareholder and first president of the company. When he retired in 1891 and Beecher died in 1892, the volume of production was about two million frames and a million pairs of lenses per year. During World War I, American Optical had eight mobile units that supplied more than 2 million glasses to troops. It provided gun sights, aviation goggles, and sunglasses to troops during World War II. In 1952, Claire McCardell and American Optical released their own version of cat eye glasses, the first eyewear line by a fashion designer. After a visit by John F. Kennedy in 1958 to American Optical Company Historic District, its original manufacturing site in Southbri ...
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Cuvette
In laboratories, a cuvette () is a small tube-like container with straight sides and a circular or square cross-section. It is sealed at one end, and made of a clear, transparent material such as plastic, glass, or fused quartz. Cuvettes are designed to hold samples for spectroscopic measurement, where a beam of light is passed through the sample within the cuvette to measure the absorbance, transmittance, fluorescence intensity, fluorescence polarization, or fluorescence lifetime of the sample. This measurement is done with a spectrophotometer. Overview Traditional ultraviolet–visible spectroscopy or fluorescence spectroscopy uses samples that are liquid. Often the sample is a solution, with the substance of interest dissolved within. The sample is placed in a cuvette and the cuvette is placed in a spectrophotometer for testing. The cuvette can be made of any material that is transparent in the range of wavelengths used in the test. The smallest cuvettes can hold 70&n ...
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Isosbestic Point
In spectroscopy, an isosbestic point is a specific wavelength, wavenumber or frequency at which the total absorbance of a sample does not change during a chemical reaction or a physical change of the sample. The word derives from two Greek words: "iso", meaning "equal", and "sbestos", meaning "extinguishable". Interpretation An isosbestic point corresponds to an absorbance A_\lambda at a fixed wavelength \lambda that remains fixed. The absorbance can be written as sum of absorbances of each species (Beer–Lambert law) A_\lambda = \ell \sum_^n \epsilon_i(\lambda) c_i \,, where c_i the concentration of species i, \ell the optical path length. By definition, an isosbestic point can be interpreted as a fixed linear combination of species concentrations, L = \sum_^n b_i c_i, \ \ \ \frac = 0 \,, i.e. an isobestic point is a conservation law. The IUPAC gold book provides as an example the reaction A + B \rightarrow c C + d D + e E \,, which will lead to an isosbestic poin ...
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National AIDS Control Organisation
The National AIDS Control Organisation (NACO), established in 1992, is a division of India's Ministry of Health and Family Welfare that provides leadership to the HIV/AIDS control programme in India through 35 HIV/AIDS Prevention and Control Societies, and is "the nodal organisation for formulation of policy and implementation of programs for prevention and control of HIV/AIDS in India.". Along with drug control authorities, NACO provides joint surveillance of blood bank licensing, blood donation activities and transfusion-transmitted infection testing and reporting. NACO also undertakes HIV estimations biennially(every 2 years) in collaboration with the Indian Council of Medical Research (ICMR) – National Institute for Research in Digital Health and Data Sciences (NIRDHDS). The first round of HIV estimation in India was done in 1998, while the last round was done in 2017. In 2010, NACO approved the TeachAids curriculum for use in India, an innovation which represented the fi ...
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Pathology
Pathology is the study of disease. The word ''pathology'' also refers to the study of disease in general, incorporating a wide range of biology research fields and medical practices. However, when used in the context of modern medical treatment, the term is often used in a narrower fashion to refer to processes and tests that fall within the contemporary medical field of "general pathology", an area that includes a number of distinct but inter-related medical specialties that diagnose disease, mostly through analysis of tissue (biology), tissue and human cell samples. Idiomatically, "a pathology" may also refer to the predicted or actual progression of particular diseases (as in the statement "the many different forms of cancer have diverse pathologies", in which case a more proper choice of word would be "Pathophysiology, pathophysiologies"). The suffix ''pathy'' is sometimes used to indicate a state of disease in cases of both physical ailment (as in cardiomyopathy) and psych ...
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