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HeLa
HeLa () is an immortalized cell line used in scientific research. It is the oldest human cell line and one of the most commonly used. HeLa cells are durable and prolific, allowing for extensive applications in scientific study. The line is derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a 31-year-old African American mother of five, after whom the line is named. Lacks died of cancer on October 4, 1951. The cells from Lacks's cancerous cervical tumor were taken without her knowledge, which was common practice in the United States at the time. Cell biologist George Otto Gey found that they could be kept alive, and developed a cell line. Previously, cells cultured from other human cells would survive for only a few days, but cells from Lacks's tumor behaved differently. History Origin In 1951, Henrietta Lacks was admitted to the Johns Hopkins Hospital with symptoms of irregular vaginal bleeding; she was subsequently treated for cerv ...
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HeLa Cells Stained With Antibody To Actin (green) , Vimentin (red) And DNA (blue)
HeLa () is an immortalized cell line used in scientific research. It is the oldest human cell line and one of the most commonly used. HeLa cells are durable and prolific, allowing for extensive applications in scientific study. The line is derived from cervical cancer cells taken on February 8, 1951, from Henrietta Lacks, a 31-year-old African American mother of five, after whom the line is named. Lacks died of cancer on October 4, 1951. The cells from Lacks's cancerous cervical tumor were taken without her knowledge, which was common practice in the United States at the time. Cell biologist George Otto Gey found that they could be kept alive, and developed a cell line. Previously, cells cultured from other human cells would survive for only a few days, but cells from Lacks's tumor behaved differently. History Origin In 1951, Henrietta Lacks was admitted to the Johns Hopkins Hospital with symptoms of irregular vaginal bleeding; she was subsequently treated for cervical ...
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Henrietta Lacks
Henrietta Lacks (born Loretta Pleasant; August 1, 1920 – October 4, 1951) Note: Some sources report her birthday as August 2, 1920, vs. August 1, 1920. was an African-American woman whose cancer cells are the source of the HeLa cell line, the first immortalized human cell line and one of the most important cell lines in medical research. An immortalized cell line reproduces indefinitely under specific conditions, and the HeLa cell line continues to be a source of invaluable medical data to the present day. Lacks was the unwitting source of these cells from a tumor biopsied during treatment for cervical cancer at Johns Hopkins Hospital in Baltimore, Maryland, in 1951. These cells were then cultured by George Otto Gey, who created the cell line known as HeLa, which is still used for medical research. As was then the practice, no consent was required to culture the cells obtained from Lacks's treatment. Neither she nor her family were compensated for the extraction or u ...
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Cell Culture
Cell culture or tissue culture is the process by which cell (biology), cells are grown under controlled conditions, generally outside of their natural environment. After cells of interest have been Cell isolation, isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator. These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or rich growth medium, medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals), growth factors, hormones, and gases (Carbon dioxide, CO2, Oxygen, O2), and regulates the physio-chemical environment (Buffer solution, pH buffer, osmotic pressure, temperature). Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. T ...
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Human Cell Line
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. After cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator. These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or rich medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals), growth factors, hormones, and gases ( CO2, O2), and regulates the physio-chemical environment (pH buffer, osmotic pressure, temperature). Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. This is typically facilitated via use of a liquid, semi-solid, or solid growth medi ...
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George Otto Gey
George Otto Gey ( ; July 6, 1899 – November 8, 1970) was the cell biologist at Johns Hopkins Hospital who is credited with propagating the HeLa cell line from Henrietta Lacks' cervical tumor. He spent over 35 years developing numerous scientific breakthroughs under the Johns Hopkins Medical School and Hospital. Early life and education Gey was born in Pittsburgh, Pennsylvania, on July 6, 1899, the son of German immigrants Frank and Emma Gey. He had an older brother and younger sister. Gey's parents immigrated from Germany, and according to the 1910 United States Census, they lived in suburban Pittsburgh. Gey graduated Peabody High School, and received undergraduate degree in biology from the University of Pittsburgh in 1920. He worked as a carpenter and a mason to help pay his way through college. Around 1926, he married Margaret K. (1900–1989), and they later moved to Baltimore where he would earn his medical degree from Johns Hopkins University. Gey was in medical school ...
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Cell Line
An immortalised cell line is a population of cells from a multicellular organism that would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells can therefore be grown for prolonged periods ''in vitro''. The mutations required for immortality can occur naturally or be intentionally induced for experimental purposes. Immortal cell lines are a very important tool for research into the biochemistry and cell biology of multicellular organisms. Immortalised cell lines have also found uses in biotechnology. An immortalised cell line should not be confused with stem cells, which can also divide indefinitely, but form a normal part of the development of a multicellular organism. Relation to natural biology and pathology There are various immortal cell lines. Some of them are normal cell lines (e.g. derived from stem cells). Other immortalised cell lines are the ''in vitro'' equivalent of ...
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Informed Consent
Informed consent is an applied ethics principle that a person must have sufficient information and understanding before making decisions about accepting risk. Pertinent information may include risks and benefits of treatments, alternative treatments, the patient's role in treatment, and their Right to refuse medical treatment, right to refuse treatment. In most systems, healthcare providers have a legal and ethical responsibility to ensure that a patient's consent is informed. This principle applies more broadly than healthcare intervention, for example to conduct research, to disclose a person's medical information, or to participate in high risk sporting and recreational activities. Within the United States, definitions of informed consent vary, and the standard required is generally determined by the state. As of 2016, nearly half of the states adopted a reasonable patient standard, in which the informed consent process is viewed from the patient's perspective. These standards ...
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Cervical Cancer
Cervical cancer is a cancer arising from the cervix or in any layer of the wall of the cervix. It is due to the abnormal growth of cells that can invade or spread to other parts of the body. Early on, typically no symptoms are seen. Later symptoms may include abnormal vaginal bleeding, pelvic pain or pain during sexual intercourse. While bleeding after sex may not be serious, it may also indicate the presence of cervical cancer. Virtually all cervical cancer cases (99%) are linked to genital human papillomavirus infection (HPV); most who have had HPV infections, however, do not develop cervical cancer. HPV 16 and 18 strains are responsible for approximately 70% of cervical cancer cases globally and nearly 50% of high grade cervical pre-cancers. Minor risk factors include smoking, a weak immune system, birth control pills, starting sex at a young age, and having many sexual partners. Genetic factors also contribute to cervical cancer risk. Cervical cancer typically develo ...
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Thermo Fisher Scientific
Thermo Fisher Scientific Inc. is an American life science and clinical research company. It is a global supplier of analytical instruments, clinical development solutions, specialty diagnostics, laboratory, pharmaceutical and biotechnology services. Based in Waltham, Massachusetts, Thermo Fisher was formed through the merger of Thermo Electron and Fisher Scientific in 2006. Thermo Fisher Scientific has acquired other reagent, consumable, instrumentation, and service providers, including Life Technologies Corporation (2013), Alfa Aesar (2015), Affymetrix (2016), FEI Company (2016), BD Advanced Bioprocessing (2018), and PPD (2021). , the company had a market capitalization of $202 billion. It ranked 97th on the ''Fortune'' 500 list based on its 2022 annual revenue of US$44.92 billion. History Predecessors and merger Thermo Electron was co-founded in 1956 by George N. Hatsopoulos and Peter M Nomikos. Hatsopoulos received a PhD from MIT in mechanical engineering ...
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Theodore Puck
Theodore Thomas Puck (September 24, 1916 – November 6, 2005) was an American geneticist born in Chicago, Illinois. He attended Chicago public schools and obtained his bachelors, masters, and doctoral degree from the University of Chicago. His PhD work was on the laws governing the impact of an electron upon an atom and his doctoral adviser was James Franck. During WW II Puck stayed at the University of Chicago. There he worked in the laboratory of Oswald H. Robertson on the study of how bacteria and viruses can spread through the air and on dust particles. After a postdoc position in the laboratory of Renato Dulbecco, Puck was recruited in 1948 to establish and chair the University of Colorado Medical School's department of biophysics. He retired from the University of Colorado Medical School in 1995 as professor emeritus, but continued to do laboratory work there until a few weeks before his death. Puck was an early pioneer of "somatic cell genetics" and single-cell plat ...
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Rebecca Skloot
Rebecca L. Skloot (born September 19, 1972) is an American science writer who specializes in science and medicine.Jessica Teisch, "Floyd Skloot & Rebecca Skloot", in '' Bookmarks'', May/June 2010. Her first book, '' The Immortal Life of Henrietta Lacks'' (2010), was one of the best-selling new books of 2010, staying on ''The New York Times'' Bestseller list for over 6 years and eventually reaching #1. It was adapted into a movie by George C. Wolfe, which premiered on HBO on April 22, 2017, and starred Rose Byrne as Skloot, and Oprah Winfrey as Lacks's daughter Deborah. Early life and education Rebecca was born in Springfield, Illinois. She is the daughter of poet, novelist, and essayist Floyd Skloot and Betsy McCarthy, a professional knitter and pattern book author. Skloot said, "in the Pacific Northwest, erroots rehalf New York Jew and half Midwestern Protestant." She received her high school diploma from Metropolitan Learning Center in Portland, Oregon. After attending ...
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