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Gene Transfer Agent-release Holin Family
Gene transfer agent-release holins (GTA-HolTC# 1.E.54 are holins which are believed to facilitate the lysis-dependent release of a gene transfer agent. Particularly the gene transfer agent of ''Rhodobacter capsulatus'' (RcGTA), which is known to be a bacteriophage-like genetic element that induces horizontal gene transfer. The promoter of the RcGTA gene was identified by Westbye ''et al''. in 2013. A representative list of members belonging to the GTA-Hol family can be found in the Transporter Classification Database with homologues found in Pseudomonadota (formerly proteobacteria) and caudovirales. See also * Gene transfer agent * Lysin Lysins, also known as endolysins or murein hydrolases, are hydrolase, hydrolytic enzymes produced by bacteriophages in order to cleave the host's cell wall during the final stage of the lytic cycle. Lysins are highly evolved enzymes that are able ... References Further reading * * * * * Holins {{membrane-protein-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Holin
Holins are a diverse group of small proteins produced by dsDNA bacteriophages in order to trigger and control the degradation of the host's cell wall at the end of the lytic cycle. Holins form pores in the host's cell membrane, allowing lysins to reach and degrade peptidoglycan, a component of bacterial cell walls. Holins have been shown to regulate the timing of lysis with great precision. Over 50 unrelated gene families encode holins, making them the most diverse group of proteins with common function. Together with lysins, holins are being studied for their potential use as antibacterial agents. While canonical holins act by forming large pores, pinholins such as the S protein of lambdoid phage 21 act by forming heptameric channels that depolarize the bacterial membrane. They are associated with SAR endolysins, which remain inactive in the periplasm prior to the depolarization of the membrane. Viruses that infect eukaryotic cells may use similar channel-forming proteins calle ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gene Transfer Agent
Gene transfer agents (GTAs) are DNA-containing virus-like particles that are produced by some bacteria and archaea and mediate horizontal gene transfer. Different GTA types have originated independently from viruses in several bacterial and archaeal lineages. These cells produce GTA particles containing short segments of the DNA present in the cell. After the particles are released from the producer cell, they can attach to related cells and inject their DNA into the cytoplasm. The DNA can then become part of the recipient cells' genome. GTAs are classified as viriforms in the ICTV taxonomy. Among the GTAs mentioned by the article, RcGTA and DsGTA are now in the family '' Rhodogtaviriformidae'', BaGTA in '' Bartogtaviriformidae'', and VSH-1 in '' Brachygtaviriformidae''. Dd1 and VTA do not yet have a classification. Discovery of gene transfer agents The first GTA system was discovered in 1974, when mixed cultures of ''Rhodobacter capsulatus'' strains produced a high frequenc ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rhodobacter Capsulatus
''Rhodobacter capsulatus'' is a species of purple bacteria, a group of bacteria that can obtain energy through photosynthesis. Its name is derived from the Latin adjective "capsulatus" ("with a chest", "encapsulated"), itself derived Latin noun "capsula" (meaning "a small box or chest"), and the associated Latin suffix for masculine nouns, "-atus" (denoting that something is "provided with" something else). Its complete genome has been sequenced and is available to the public. Discovery The discover Hans Molisch, a Czech-Austrian botanist. The microorganism, then named ''Rhodonostoc capsulatum'', was identified in 1907 in his book ''Die Purpurbakterien nach neuen Untersuchungen''. C. B. van Niel then characterized the species further in 1944 where it was renamed ''Rhodopseudomonas capsulata''. Van Niel initially described 16 strains of ''R. capsulata'' that he was able to culture from mud samples collected in California and Cuba. In 1984, the species would be reclassified as ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Horizontal Gene Transfer
Horizontal gene transfer (HGT) or lateral gene transfer (LGT) is the movement of genetic material between organisms other than by the ("vertical") transmission of DNA from parent to offspring (reproduction). HGT is an important factor in the evolution of many organisms. HGT is influencing scientific understanding of higher-order evolution while more significantly shifting perspectives on bacterial evolution. Horizontal gene transfer is the primary mechanism for the spread of antibiotic resistance in bacteria, and plays an important role in the evolution of bacteria that can degrade novel compounds such as human-created Bactericide, pesticides and in the evolution, maintenance, and transmission of virulence. It often involves Temperateness (virology), temperate bacteriophages and plasmids. Genes responsible for antibiotic resistance in one species of bacteria can be transferred to another species of bacteria through various mechanisms of HGT such as Transformation (genetics), tr ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Transporter Classification Database
The Transporter Classification Database (or TCDB) is an International Union of Biochemistry and Molecular Biology (IUBMB)-approved classification system for membrane transport proteins, including ion channels. Classification The upper level of classification and a few examples of proteins with known 3D structure: 1. Channels and pores 1.A α-type channels * 1.A.1 Voltage-gated ion channel superfamily * 1.A.2 Inward-rectifier K+ channel family * 1.A.3 Ryanodine-inositol-1,4,5-trisphosphate receptor Ca2+ channel family * 1.A.4 Transient receptor potential Ca2+ channel family * 1.A.5 Polycystin cation channel family * 1.A.6 Epithelial Na+ channel family * 1.A.7 ATP-gated P2X receptor cation channel family * 1.A.8 Major intrinsic protein superfamily * 1.A.9 Neurotransmitter receptor, Cys loop, ligand-gated ion channel family * 1.A.10 Glutamate-gated ion channel family of neurotransmitter receptors * 1.A.11 Ammonium channel transporter family * 1.A.12 Intracellular chlorid ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pseudomonadota
Pseudomonadota (synonym "Proteobacteria") is a major phylum of gram-negative bacteria. Currently, they are considered the predominant phylum within the domain of bacteria. They are naturally found as pathogenic and free-living (non- parasitic) genera. The phylum comprises six classes ''Acidithiobacillia, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Hydrogenophilia'', and '' Zetaproteobacteria.'' The Pseudomonadota are widely diverse, with differences in morphology, metabolic processes, relevance to humans, and ecological influence. Classification American microbiologist Carl Woese established this grouping in 1987, calling it informally the "purple bacteria and their relatives". The group was later formally named the 'Proteobacteria' after the Greek god Proteus, who was known to assume many forms. In 2021 the International Committee on Systematics of Prokaryotes designated the synonym Pseudomonadota, and renamed many other prokaryotic phyla as well. Th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Caudovirales
''Caudoviricetes'' is a class of viruses known as tailed viruses and head-tail viruses (''cauda'' is Latin for "tail"). It is the sole representative of its own phylum, ''Uroviricota'' (from ''ouros'' (ουρος), a Greek word for "tailed" + -viricota). Under the Baltimore classification scheme, the ''Caudoviricetes'' are group I viruses as they have double stranded DNA (dsDNA) genomes, which can be anywhere from 18,000 base pairs to 500,000 base pairs in length. The virus particles have a distinct shape; each virion has an icosahedral head that contains the viral genome, and is attached to a flexible tail by a connector protein. The order encompasses a wide range of viruses, many containing genes of similar nucleotide sequence and function. However, some tailed bacteriophage genomes can vary quite significantly in nucleotide sequence, even among the same genus. Due to their characteristic structure and possession of potentially homologous genes, it is believed these viruse ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gene Transfer Agent
Gene transfer agents (GTAs) are DNA-containing virus-like particles that are produced by some bacteria and archaea and mediate horizontal gene transfer. Different GTA types have originated independently from viruses in several bacterial and archaeal lineages. These cells produce GTA particles containing short segments of the DNA present in the cell. After the particles are released from the producer cell, they can attach to related cells and inject their DNA into the cytoplasm. The DNA can then become part of the recipient cells' genome. GTAs are classified as viriforms in the ICTV taxonomy. Among the GTAs mentioned by the article, RcGTA and DsGTA are now in the family '' Rhodogtaviriformidae'', BaGTA in '' Bartogtaviriformidae'', and VSH-1 in '' Brachygtaviriformidae''. Dd1 and VTA do not yet have a classification. Discovery of gene transfer agents The first GTA system was discovered in 1974, when mixed cultures of ''Rhodobacter capsulatus'' strains produced a high frequenc ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Lysin
Lysins, also known as endolysins or murein hydrolases, are hydrolase, hydrolytic enzymes produced by bacteriophages in order to cleave the host's cell wall during the final stage of the lytic cycle. Lysins are highly evolved enzymes that are able to target one of the five bonds in peptidoglycan (murein), the main component of bacterial cell walls, which allows the release of progeny virions from the lysed cell. Cell-wall-containing Archaea are also lysed by specialized pseudomurein-cleaving lysins, while most archaeal viruses employ alternative mechanisms. Similarly, not all bacteriophages synthesize lysins: some small single-stranded DNA and RNA phages produce membrane proteins that activate the host's Autolysis (biology), autolytic mechanisms such as autolysins. Lysins were first used therapeutically in 2001 by the Fischetti lab (see below) and are now being used as antibacterial agents due to their high effectiveness and specificity in comparison with antibiotics, which are sus ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
