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Gating (cytometry)
A gate in cytometry is a set of value limits (boundaries) that serve to isolate a specific group of cytometric events from a large set. Gates can be defined by discrimination analysis, or can simply be drawn around a given set of data points on a printout and then converted to a computer-useful form. Gates can be implemented with a physical blinder. Gates may be used either to selectively gather data or to segregate data for analysis. Division Gates are divided mathematically into inclusive gates and exclusive gates. Inclusive gates select data that falls within the limits set, while exclusive gates select data that falls outside the limits. Live gate A live gate is a term used for a process that prevents the acquisition by the computer of non-selected data from the flow cytometer Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of Cell (biology), cells or particles. In this process, a sample containing cells or ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Flow Cytometric Gating By Side Scatter And CD45
Flow may refer to: Science and technology * Fluid flow, the motion of a gas or liquid * Flow (geomorphology), a type of mass wasting or slope movement in geomorphology * Flow (mathematics), a group action of the real numbers on a set * Flow (psychology), a mental state of being fully immersed and focused * Flow, a spacecraft of NASA's GRAIL program Computing * Flow network, graph-theoretic version of a mathematical flow * Flow analysis * Calligra Flow, free diagramming software * Dataflow, a broad concept in computer systems with many different meanings * Microsoft Flow (renamed to Power Automate in 2019), a workflow toolkit in Microsoft Dynamics * Neos Flow, a free and open source web application framework written in PHP * webMethods Flow, a graphical programming language * FLOW (programming language), an educational programming language from the 1970s * Flow (web browser), a web browser with a proprietary rendering engine Arts, entertainment and media * ''Flow'' (journal ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cytometry
Cytometry is the measurement of number and characteristics of cells. Variables that can be measured by cytometric methods include cell size, cell count, cell morphology (shape and structure), cell cycle phase, DNA content, and the existence or absence of specific proteins on the cell surface or in the cytoplasm. Cytometry is used to characterize and count blood cells in common blood tests such as the complete blood count. In a similar fashion, cytometry is also used in cell biology research and in medical diagnostics to characterize cells in a wide range of applications associated with diseases such as cancer and AIDS. Cytometric devices Image cytometers Image cytometry is the oldest form of cytometry. Image cytometers operate by statically imaging a large number of cells using optical microscopy. Prior to analysis, cells are commonly stained to enhance contrast or to detect specific molecules by labeling these with fluorochromes. Traditionally, cells are viewed within a hemocyt ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Flow Cytometer
Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer. Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include: * Cell counting * Cell sorting * Determining cell characteristics and function * Detecting microorganisms * Biomarker detection * Protein engineering detection * Diagnosis of health disorders such as blood cancers * Measuring ge ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Flow Cytometry
Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. Cells are often labeled with fluorescent markers so light is absorbed and then emitted in a band of wavelengths. Tens of thousands of cells can be quickly examined and the data gathered are processed by a computer. Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include: * Cell counting * Cell sorting * Determining cell characteristics and function * Detecting microorganisms * Biomarker detection * Protein engineering detection * Diagnosis of health disorders such as blood cancers * Measur ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
