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Elutriation
Elutriation is a process for separating particles based on their size, shape and density, using a stream of gas or liquid flowing in a direction usually opposite to the direction of sedimentation. This method is mainly used for particles smaller than 1 μm. The smaller or lighter particles rise to the top (overflow) because their terminal sedimentation velocities are lower than the velocity of the rising fluid. The terminal velocity of any particle in any medium can be calculated using Stokes' law if the particle's Reynolds number is below 0.2. Counterflow centrifugation elutriation is a related technique to separate cells. Air classification An air elutriator is a simple device which can separate particles into two or more groups. Material may be separated by means of an elutriator, which consists of a vertical tube up which fluid is passed at a controlled velocity. When the particles are introduced, often through a side tube, the smaller particles are carried over in ...
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Counterflow Centrifugation Elutriation
Counterflow centrifugal elutriation (CCE) is a liquid clarification technique. This method enables scientists to separate different cells with different sizes. Since cell size is correlated with cell cycle stages this method also allows the separation of cells at different stages of the cell cycle. Principle The key concept is that larger cells tend to stay within the flowing buffer solution while smaller cells will be washed away follow the buffer solution (different sedimentation property within the buffer solution), and cells will have different sedimentation properties in different cell cycle stages. The basic principle of separating the cells inside CCE is the balance between centripetal and the counter flow drag force. When the cells enter the elutriation chamber, all the cells will stay at the outer edge of the chamber due to centrifugal force. Then when the flow rate of the buffer solution increases, the solution tends to push the cells towards the middle of the CCE. When ...
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1900 Circa Avis Original Askania-Sichter H
Nineteen or 19 may refer to: * 19 (number) * One of the years 19 BC, AD 19, 1919, 2019 Films * ''19'' (film), a 2001 Japanese film * ''Nineteen'' (1987 film), a 1987 science fiction film * ''19-Nineteen'', a 2009 South Korean film * ''Diciannove'', a 2024 Italian drama film informally referred to as "Nineteen" in some sources Science * Potassium, an alkali metal * 19 Fortuna, an asteroid Music * 19 (band), a Japanese pop music duo Albums * ''19'' (Adele album), 2008 * ''19'', a 2003 album by Alsou * ''19'', a 2006 album by Evan Yo * ''19'', a 2018 album by MHD * ''19'', one half of the double album ''63/19'' by Kool A.D. * ''Number Nineteen'', a 1971 album by American jazz pianist Mal Waldron * ''XIX'' (EP), a 2019 EP by 1the9 Songs * "19" (song), a 1985 song by British musician Paul Hardcastle * "Stone in Focus", officially "#19", a composition by Aphex Twin * "Nineteen", a song from the 1992 album ''Refugee'' by Bad4Good * "Nineteen", a song from the 2001 alb ...
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Liquid
Liquid is a state of matter with a definite volume but no fixed shape. Liquids adapt to the shape of their container and are nearly incompressible, maintaining their volume even under pressure. The density of a liquid is usually close to that of a solid, and much higher than that of a gas. Therefore, liquid and solid are classified as condensed matter. Meanwhile, since both liquids and gases can flow, they are categorized as fluids. A liquid is composed of atoms or molecules held together by intermolecular bonds of intermediate strength. These forces allow the particles to move around one another while remaining closely packed. In contrast, solids have particles that are tightly bound by strong intermolecular forces, limiting their movement to small vibrations in fixed positions. Gases, on the other hand, consist of widely spaced, freely moving particles with only weak intermolecular forces. As temperature increases, the molecules in a liquid vibrate more intensely, causi ...
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Terminal Velocity
Terminal velocity is the maximum speed attainable by an object as it falls through a fluid (air is the most common example). It is reached when the sum of the drag force (''Fd'') and the buoyancy is equal to the downward force of gravity (''FG'') acting on the object. Since the net force on the object is zero, the object has zero acceleration. For objects falling through air at normal pressure, the buoyant force is usually dismissed and not taken into account, as its effects are negligible. As the speed of an object increases, so does the drag force acting on it, which also depends on the substance it is passing through (for example air or water). At some speed, the drag or force of resistance will be equal to the gravitational pull on the object. At this point the object stops accelerating and continues falling at a constant speed called the terminal velocity (also called settling velocity). An object moving downward faster than the terminal velocity (for example because it ...
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Stokes' Law
In fluid dynamics, Stokes' law gives the frictional force – also called drag force – exerted on spherical objects moving at very small Reynolds numbers in a viscous fluid. It was derived by George Gabriel Stokes in 1851 by solving the Stokes flow limit for small Reynolds numbers of the Navier–Stokes equations.Batchelor (1967), p. 233. Statement of the law The force of viscosity on a small sphere moving through a viscous fluid is given by: :_ = - 6 \pi \mu R where (in SI units): * _ is the frictional force – known as Stokes' drag – acting on the interface between the fluid and the particle (newtons, kg m s−2); * (some authors use the symbol ) is the dynamic viscosity ( Pascal-seconds, kg m−1 s−1); * is the radius of the spherical object (meters); * is the flow velocity relative to the object (meters per second). Note the minus sign in the equation, the drag force points in the opposite direction to the relative velocity: drag opposes the motion. Stokes' ...
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Reynolds Number
In fluid dynamics, the Reynolds number () is a dimensionless quantity that helps predict fluid flow patterns in different situations by measuring the ratio between Inertia, inertial and viscous forces. At low Reynolds numbers, flows tend to be dominated by laminar flow, laminar (sheet-like) flow, while at high Reynolds numbers, flows tend to be turbulence, turbulent. The turbulence results from differences in the fluid's speed and direction, which may sometimes intersect or even move counter to the overall direction of the flow (Eddy (fluid dynamics), eddy currents). These eddy currents begin to churn the flow, using up energy in the process, which for liquids increases the chances of cavitation. The Reynolds number has wide applications, ranging from liquid flow in a pipe to the passage of air over an aircraft wing. It is used to predict the transition from laminar–turbulent transition, laminar to turbulent flow and is used in the scaling of similar but different-sized fl ...
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Pyroclastic Flow
A pyroclastic flow (also known as a pyroclastic density current or a pyroclastic cloud) is a fast-moving current of hot gas and volcanic matter (collectively known as tephra) that flows along the ground away from a volcano at average speeds of but is capable of reaching speeds up to . The gases and tephra can reach temperatures of about . Pyroclastic flows are the deadliest of all volcanic hazards and are produced as a result of certain explosive eruptions; they normally touch the ground and hurtle downhill or spread laterally under gravity. Their speed depends upon the density of the current, the volcanic output rate, and the gradient of the slope. Origin of term The word ''pyroclast'' is derived from the Greek (''pýr''), meaning "fire", and (''klastós''), meaning "broken in pieces". A name for pyroclastic flows that glow red in the dark is (French, "burning cloud"); this was notably used to describe the disastrous 1902 eruption of Mount Pelée on Martinique, a Frenc ...
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Volcano
A volcano is commonly defined as a vent or fissure in the crust of a planetary-mass object, such as Earth, that allows hot lava, volcanic ash, and gases to escape from a magma chamber below the surface. On Earth, volcanoes are most often found where tectonic plates are diverging or converging, and because most of Earth's plate boundaries are underwater, most volcanoes are found underwater. For example, a mid-ocean ridge, such as the Mid-Atlantic Ridge, has volcanoes caused by divergent tectonic plates whereas the Pacific Ring of Fire has volcanoes caused by convergent tectonic plates. Volcanoes resulting from divergent tectonic activity are usually non-explosive whereas those resulting from convergent tectonic activity cause violent eruptions."Mid-ocean ridge tectonics, volcanism and geomorphology." Geology 26, no. 455 (2001): 458. https://macdonald.faculty.geol.ucsb.edu/papers/Macdonald%20Mid-Ocean%20Ridge%20Tectonics.pdf Volcanoes can also form where there is str ...
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Fission Yeast
''Schizosaccharomyces pombe'', also called "fission yeast", is a species of yeast used in traditional brewing and as a model organism in molecular and cell biology. It is a unicellular eukaryote, whose cells are rod-shaped. Cells typically measure 3 to 4 micrometres in diameter and 7 to 14 micrometres in length. Its genome, which is approximately 14.1 million base pairs, is estimated to contain 4,970 protein-coding genes and at least 450 non-coding RNAs. These cells maintain their shape by growing exclusively through the cell tips and divide by medial fission to produce two daughter cells of equal size, which makes them a powerful tool in cell cycle research. Fission yeast was isolated in 1893 by Paul Lindner from East African millet beer. The species name ''pombe'' is the Swahili word for beer. It was first developed as an experimental model in the 1950s: by Urs Leupold for studying genetics, and by Murdoch Mitchison for studying the cell cycle. Paul Nurse, a fission yeast ...
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Cell Cycle
The cell cycle, or cell-division cycle, is the sequential series of events that take place in a cell (biology), cell that causes it to divide into two daughter cells. These events include the growth of the cell, duplication of its DNA (DNA replication) and some of its organelles, and subsequently the partitioning of its cytoplasm, chromosomes and other components into two daughter cells in a process called cell division. In eukaryotic cells (having a cell nucleus) including animal, plant, fungal, and protist cells, the cell cycle is divided into two main stages: interphase, and the M phase that includes mitosis and cytokinesis. During interphase, the cell grows, accumulating nutrients needed for mitosis, and replicates its DNA and some of its organelles. During the M phase, the replicated Chromosome, chromosomes, organelles, and cytoplasm separate into two new daughter cells. To ensure the proper replication of cellular components and division, there are control mechanisms kno ...
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Meiofauna
Meiobenthos, also called meiofauna, are small benthic invertebrates that live in marine or freshwater environments, or both. The term ''meiofauna'' loosely defines a group of organisms by their sizelarger than microfauna but smaller than macrofaunarather than by their taxonomy. This fauna includes both animals that turn into macrofauna later in life, and those small enough to belong to the meiobenthos their entire life. In marine environments there can be thousands of individuals in 10 cubic centimeters of sediment, and counts animals like nematodes, copepods, rotifers, tardigrades and ostracods, but protists like ciliates and foraminifers within the size range of the meiobenthos are also often included. In practice, the term usually includes organisms that can pass through a 1 mm mesh but are retained by a 45 μm mesh, though exact dimensions may vary. Whether an organism will pass through a 1 mm mesh also depends upon whether it is alive or dead at the time of so ...
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Sediment
Sediment is a solid material that is transported to a new location where it is deposited. It occurs naturally and, through the processes of weathering and erosion, is broken down and subsequently sediment transport, transported by the action of wind, water, or ice or by the force of gravity acting on the particles. For example, sand and silt can be carried in suspension (chemistry), suspension in river water and on reaching the sea bed deposited by sedimentation; if buried, they may eventually become sandstone and siltstone (sedimentary rocks) through lithification. Sediments are most often transported by water (fluvial, fluvial processes), but also wind (aeolian processes) and glaciers. Beach sands and stream channel, river channel deposits are examples of fluvial transport and deposition (geology), deposition, though sediment also often settles out of slow-moving or standing water in lakes and oceans. Desert sand dunes and loess are examples of aeolian transport and deposition. ...
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