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Eadie–Hofstee Diagram
In biochemistry Biochemistry or biological chemistry is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology ..., an Eadie–Hofstee diagram (more usually called an Eadie–Hofstee plot) is a graphical representation of the Michaelis–Menten equation in enzyme kinetics. It has been known by various different names, including Eadie plot, Hofstee plot and Augustinsson plot. Attribution to Woolf is often omitted, because although Haldane and Stern credited Woolf with the underlying equation, it was just one of the three linear transformations of the Michaelis–Menten equation that they initially introduced. However, Haldane indicated latter that Woolf had indeed found the three linear forms: "''In 1932, Dr. Kurt Stern published a German translation of my book "Enzymes", with numerous additions to the English text. On pp. 119-120 ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biochemistry
Biochemistry or biological chemistry is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology and metabolism. Over the last decades of the 20th century, biochemistry has become successful at explaining living processes through these three disciplines. Almost all areas of the life sciences are being uncovered and developed through biochemical methodology and research.Voet (2005), p. 3. Biochemistry focuses on understanding the chemical basis which allows biological molecules to give rise to the processes that occur within living cells and between cells, Karp (2009), p. 2. in turn relating greatly to the understanding of tissues and organs, as well as organism structure and function. Miller (2012). p. 62. Biochemistry is closely related to molecular biology, which is the study of the molecular mechanisms of biological phenom ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Independent Variable
Dependent and independent variables are variables in mathematical modeling, statistical modeling and experimental sciences. Dependent variables receive this name because, in an experiment, their values are studied under the supposition or demand that they depend, by some law or rule (e.g., by a mathematical function), on the values of other variables. Independent variables, in turn, are not seen as depending on any other variable in the scope of the experiment in question. In this sense, some common independent variables are time, space, density, mass, fluid flow rate, and previous values of some observed value of interest (e.g. human population size) to predict future values (the dependent variable). Of the two, it is always the dependent variable whose variation is being studied, by altering inputs, also known as regressors in a statistical context. In an experiment, any variable that can be attributed a value without attributing a value to any other variable is called an in ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biochemistry
Biochemistry or biological chemistry is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology and metabolism. Over the last decades of the 20th century, biochemistry has become successful at explaining living processes through these three disciplines. Almost all areas of the life sciences are being uncovered and developed through biochemical methodology and research.Voet (2005), p. 3. Biochemistry focuses on understanding the chemical basis which allows biological molecules to give rise to the processes that occur within living cells and between cells, Karp (2009), p. 2. in turn relating greatly to the understanding of tissues and organs, as well as organism structure and function. Miller (2012). p. 62. Biochemistry is closely related to molecular biology, which is the study of the molecular mechanisms of biological phenom ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Enzyme Kinetics
Enzyme kinetics is the study of the rates of enzyme-catalysed chemical reactions. In enzyme kinetics, the reaction rate is measured and the effects of varying the conditions of the reaction are investigated. Studying an enzyme's kinetics in this way can reveal the catalytic mechanism of this enzyme, its role in metabolism, how its activity is controlled, and how a drug or a modifier (inhibitor or activator) might affect the rate. An enzyme (E) is typically a protein molecule that promotes a reaction of another molecule, its substrate (S). This binds to the active site of the enzyme to produce an enzyme-substrate complex ES, and is transformed into an enzyme-product complex EP and from there to product P, via a transition state ES*. The series of steps is known as the mechanism: : E + S ⇄ ES ⇄ ES* ⇄ EP ⇄ E + P This example assumes the simplest case of a reaction with one substrate and one product. Such cases exist: for example, a mutase such as phosphoglucomutase ca ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Diagrams
A diagram is a symbolic representation of information using visualization techniques. Diagrams have been used since prehistoric times on walls of caves, but became more prevalent during the Enlightenment. Sometimes, the technique uses a three-dimensional visualization which is then projected onto a two-dimensional surface. The word ''graph'' is sometimes used as a synonym for diagram. Overview The term "diagram" in its commonly used sense can have a general or specific meaning: * ''visual information device'' : Like the term "illustration", "diagram" is used as a collective term standing for the whole class of technical genres, including graphs, technical drawings and tables. * ''specific kind of visual display'' : This is the genre that shows qualitative data with shapes that are connected by lines, arrows, or other visual links. In science the term is used in both ways. For example, Anderson (1997) stated more generally: "diagrams are pictorial, yet abstract, representa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Hanes–Woolf Plot
In biochemistry, a Hanes–Woolf plot, Hanes plot, or plot of a/v against a, is a graphical representation of enzyme kinetics in which the ratio of the initial substrate concentration a to the reaction velocity v is plotted against a. It is based on the rearrangement of the Michaelis–Menten equation shown below: : = + where K_\mathrm is the Michaelis constant and V is the limiting rate. J B S Haldane stated, reiterating what he and K. G. Stern had written in their book, that this rearrangement was due to Barnet Woolf. However, it was just one of three transformations introduced by Woolf, who did not use it as the basis of a plot. There is therefore no strong reason for attaching his name to it. It was first published by C. S. Hanes, though he did not use it as plot either. Hanes said that the use of linear regression to determine kinetic parameters from this type of linear transformation is flawed, because it generates the best fit between observed and calculated ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Linear Regression
In statistics, linear regression is a linear approach for modelling the relationship between a scalar response and one or more explanatory variables (also known as dependent and independent variables). The case of one explanatory variable is called '' simple linear regression''; for more than one, the process is called multiple linear regression. This term is distinct from multivariate linear regression, where multiple correlated dependent variables are predicted, rather than a single scalar variable. In linear regression, the relationships are modeled using linear predictor functions whose unknown model parameters are estimated from the data. Such models are called linear models. Most commonly, the conditional mean of the response given the values of the explanatory variables (or predictors) is assumed to be an affine function of those values; less commonly, the conditional median or some other quantile is used. Like all forms of regression analysis, linear regressio ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Propagation Of Uncertainty
In statistics, propagation of uncertainty (or propagation of error) is the effect of variables' uncertainties (or errors, more specifically random errors) on the uncertainty of a function based on them. When the variables are the values of experimental measurements they have uncertainties due to measurement limitations (e.g., instrument precision) which propagate due to the combination of variables in the function. The uncertainty ''u'' can be expressed in a number of ways. It may be defined by the absolute error . Uncertainties can also be defined by the relative error , which is usually written as a percentage. Most commonly, the uncertainty on a quantity is quantified in terms of the standard deviation, , which is the positive square root of the variance. The value of a quantity and its error are then expressed as an interval . If the statistical probability distribution of the variable is known or can be assumed, it is possible to derive confidence limits to describ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Abscissa
In common usage, the abscissa refers to the (''x'') coordinate and the ordinate refers to the (''y'') coordinate of a standard two-dimensional graph. The distance of a point from the y-axis, scaled with the x-axis, is called abscissa or x coordinate of the point. The distance of a point from x-axis scaled with the y-axis is called ordinate. For example, if (x, y) is an ordered pair in the Cartesian plane, then the first coordinate in the plane (x) is called the abscissa and the second coordinate (y) is the ordinate. In mathematics, the abscissa (; plural ''abscissae'' or ''abscissas'') and the ordinate are respectively the first and second coordinate of a point in a Cartesian coordinate system: :abscissa \equiv x-axis (horizontal) coordinate :ordinate \equiv y-axis (vertical) coordinate Usually these are the horizontal and vertical coordinates of a point in plane, the rectangular coordinate system. An ordered pair consists of two terms—the abscissa (horizontal, usuall ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Michaelis–Menten Kinetics
In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. It is named after German biochemist Leonor Michaelis and Canadian physician Maud Menten. The model takes the form of an equation describing the rate of enzymatic reactions, by relating reaction rate v (rate of formation of product, ce P/math>) to ce S/math>, the concentration of a substrate ''S''. Its formula is given by : v = \frac = V_\max \frac This equation is called the Michaelis–Menten equation. Here, V_\max represents the maximum rate achieved by the system, happening at saturating substrate concentration for a given enzyme concentration. When the value of the Michaelis constant K_\mathrm is numerically equal to the substrate concentration, then the reaction rate is half of V_\max. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. Model In 1901, Fre ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ordinate
In common usage, the abscissa refers to the (''x'') coordinate and the ordinate refers to the (''y'') coordinate of a standard two-dimensional graph. The distance of a point from the y-axis, scaled with the x-axis, is called abscissa or x coordinate of the point. The distance of a point from x-axis scaled with the y-axis is called ordinate. For example, if (x, y) is an ordered pair in the Cartesian plane, then the first coordinate in the plane (x) is called the abscissa and the second coordinate (y) is the ordinate. In mathematics, the abscissa (; plural ''abscissae'' or ''abscissas'') and the ordinate are respectively the first and second coordinate of a point in a Cartesian coordinate system: :abscissa \equiv x-axis (horizontal) coordinate :ordinate \equiv y-axis (vertical) coordinate Usually these are the horizontal and vertical coordinates of a point in plane, the rectangular coordinate system. An ordered pair consists of two terms—the abscissa (horizontal, usuall ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
