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Deinococcota
Deinococcota (synonym, Deinococcus-Thermus) is a phylum of bacteria with a single class, ''Deinococci'', that are highly resistant to environmental hazards, also known as extremophiles. These bacteria have thick cell walls that give them gram-positive stains, but they include a second membrane and so are closer in structure to those of gram-negative bacteria. Taxonomy The phylum Deinococcota consists of a single class ( Deinococci) and two orders: * The Deinococcales include two families ( Deinococcaceae and Trueperaceae), with three genera, '' Deinococcus'', '' Deinobacterium'' and '' Truepera''.Garrity GM, Holt JG. (2001) Phylum BIV. "Deinococcus–Thermus". In: Bergey’s manual of systematic bacteriology, pp. 395-420. Eds D. R. Boone, R. W. Castenholz. Springer-: New York.Garrity GM, Bell JA, Lilburn TG. (2005) Phylum BIV. The revised road map to the Manual. In: Bergey’s manual of systematic bacteriology, pp. 159-220. Eds Brenner DJ, Krieg NR, Staley JT, Garrity GM. Sprin ...
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Trueperaceae
''Truepera'' is the only genus of bacteria in the family Trueperaceae from the phylum Deinococcota. It has one species, ''Truepera radiovictrix''. The following points accounts for the characteristics of the only known species: * These strains form orange/red colonies and have spherical-shaped cells. * Optimum growth temperature of about 50 °C. * Optimum pH for growth between about 7.5 and 9.5, and do not grow at pH below 6.5 or above pH 11.2. * They are extremely resistant to ionizing radiation. For instance up to 60% of the cells can survive even after being exposed to 5.0 kGy * These strains are chemo-organotrophic and aerobic; do not grow in ''Thermus'' medium under anaerobic conditions with or without nitrate as electron acceptor and glucose as a source of carbon and energy, but ferment glucose to d-lactate without formation of gas. They assimilate a large variety of sugars, organic acids and amino acids. The sole species of this genus, ''Truepera radiovictrix,'' c ...
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Thermales
Thermaceae is a family of bacteria belonging to the phylum Deinococcota. It is the only family in the order Thermales. They are particularly resistant to heat, and live in the benthic zone of the Gulf of Mexico. Members of the order ''Thermales'' can be distinguished from all other bacteria through molecular signatures consisting of conserved signature indels (CSIs) and conserved signature proteins (CSPs) that are exclusively present in members of this order. Specifically, six CSIs were identified in the following proteins: DNA topoisomerase I, ABC transporter permease, citrate synthase, phosphoribosylformylglycinamidine synthase, pyruvate dehydrogenase and alpha-glucan phosphorylase. 51 CSPs were also found to be exclusively shared by members of this order. In addition, a 76-aminoacid CSI is present in the protein SecA preprotein translocase which is specific to all members of the order ''Thermales'' as well as ''Hydrogenibacillus schlegelii'' (also an thermophilic species). ...
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Thermus
''Thermus'' is a genus of thermophilic bacteria. It is one of several bacteria belonging to the ''Deinococcota'' phylum. According to comparative analysis of 16S rRNA, this is one of the most ancient group of bacteria. ''Thermus'' species can be distinguished from other genera in the family ''Thermaceae'' as well as all other bacteria by the presence of eight conserved signature indels found in proteins such as adenylate kinase and replicative DNA helicase as well as 14 conserved signature proteins that are exclusively shared by members of this genus. Phylogeny The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and the National Center for Biotechnology Information (NCBI). Between all its species, '' T. thermophilus'' has a special importance as a model organism for basic and applied research. Species incertae sedis: * "''T. anatoliensis''" Kacagan et al. 2016 * "''T. caldophilus''" Taguchi et al. 1983 * "''T. ...
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Meiothermus
''Meiothermus'' is a genus of '' Deinococcota'' bacteria. Members of ''Meiothermus'' can be reliably distinguished from other genera in the family '' Thermaceae'' as well as all other bacteria by the presence of three conserved signature indels (CSIs) found in the proteins: 5-methyltetrahydrofolate–homocysteine methyltransferase, cadmium transporter and polynucleotide phosphorylase and are exclusively shared by species of this genus. ''Meiothermus'' is also different than the '' Thermus'' genus, which it was originally a member of, in their optimum growth temperatures, with ''Meiothermus'' being able to grow in colder environments. ''Meiothermus'' was first isolated with ''Thermus'' in alkaline and neutral hot springs in Kamchatka, Russia and Yellowstone National Park, USA. Characteristics ''Meiothermus'' species of bacteria are gram-negative and are primarily chemoorganoheterotrophs, and some species can use nitrate as an electron acceptor. The ''Meiothermus'' genus was o ...
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Truepera Radiovictrix
''Truepera'' is the only genus of bacteria in the family Trueperaceae from the phylum Deinococcota. It has one species, ''Truepera radiovictrix''. The following points accounts for the characteristics of the only known species: * These strains form orange/red colonies and have spherical-shaped cells. * Optimum growth temperature of about 50 °C. * Optimum pH for growth between about 7.5 and 9.5, and do not grow at pH below 6.5 or above pH 11.2. * They are extremely resistant to ionizing radiation. For instance up to 60% of the cells can survive even after being exposed to 5.0 kGy * These strains are chemo-organotrophic and aerobic; do not grow in ''Thermus'' medium under anaerobic conditions with or without nitrate as electron acceptor and glucose as a source of carbon and energy, but ferment glucose to d-lactate without formation of gas. They assimilate a large variety of sugars, organic acids and amino acids. The sole species of this genus, ''Truepera radiovictrix,'' ca ...
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Truepera
''Truepera'' is the only genus of bacteria in the family Trueperaceae from the phylum Deinococcota. It has one species, ''Truepera radiovictrix''. The following points accounts for the characteristics of the only known species: * These strains form orange/red colonies and have spherical-shaped cells. * Optimum growth temperature of about 50 °C. * Optimum pH for growth between about 7.5 and 9.5, and do not grow at pH below 6.5 or above pH 11.2. * They are extremely resistant to ionizing radiation. For instance up to 60% of the cells can survive even after being exposed to 5.0 kGy * These strains are chemo-organotrophic and aerobic; do not grow in ''Thermus'' medium under anaerobic conditions with or without nitrate as electron acceptor and glucose as a source of carbon and energy, but ferment glucose to d-lactate without formation of gas. They assimilate a large variety of sugars, organic acids and amino acids. The sole species of this genus, ''Truepera radiovictrix,'' ...
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Thermus Aquaticus
''Thermus aquaticus'' is a species of bacteria that can tolerate high temperatures, one of several thermophile, thermophilic bacteria that belong to the ''Deinococcota'' phylum. It is the source of the heat-resistant enzyme Taq polymerase, ''Taq'' DNA polymerase, one of the most important enzymes in molecular biology because of its use in the polymerase chain reaction (PCR) DNA amplification technique. History When studies of biological organisms in hot springs began in the 1960s, scientists thought that the life of thermophilic bacteria could not be sustained in temperatures above about . Soon, however, it was discovered that many bacteria in different springs not only survived, but also thrived in higher temperatures. In 1969, Thomas D. Brock and Hudson Freeze of Indiana University Bloomington, Indiana University reported a new species of thermophile, thermophilic bacteria which they named ''Thermus aquaticus''. The bacterium was first isolated from Mushroom Spring in the Geot ...
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Thermaceae
Thermaceae is a family of bacteria belonging to the phylum Deinococcota. It is the only family in the order Thermales. They are particularly resistant to heat, and live in the benthic zone of the Gulf of Mexico. Members of the order ''Thermales'' can be distinguished from all other bacteria through molecular signatures consisting of conserved signature indels (CSIs) and conserved signature proteins (CSPs) that are exclusively present in members of this order. Specifically, six CSIs were identified in the following proteins: DNA topoisomerase I, ABC transporter permease, citrate synthase, phosphoribosylformylglycinamidine synthase, pyruvate dehydrogenase and alpha-glucan phosphorylase. 51 CSPs were also found to be exclusively shared by members of this order. In addition, a 76-aminoacid CSI is present in the protein SecA preprotein translocase which is specific to all members of the order ''Thermales'' as well as ''Hydrogenibacillus schlegelii'' (also an thermophilic species). Ba ...
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Gram-positive
In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. The Gram stain is used by microbiologists to place bacteria into two main categories, gram-positive (+) and gram-negative bacteria, gram-negative (−). Gram-positive bacteria have a thick layer of peptidoglycan within the cell wall, and gram-negative bacteria have a thin layer of peptidoglycan. Gram-positive bacteria retain the crystal violet stain used in the test, resulting in a purple color when observed through an optical microscope. The thick layer of peptidoglycan in the bacterial cell wall retains the Stain (biology), stain after it has been fixed in place by iodine. During the decolorization step, the decolorizer removes crystal violet from all other cells. Conversely, gram-negative bacteria cannot retain the violet stain after the decolorization ...
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Deinobacterium
''Deinobacterium'' is a genus in the '' Deinococcota'' phylum (Bacteria). Not to be confused with ''Deinobacter'', a disused name for '' Deinococcus''. Etymology The name ''Deinobacterium'' derives from: Greek adjective ''deinos (δεινός)'', dreadful, strange; Neo-Latin neuter gender noun ''bacterium'', nominally meaning "a rod", but in effect meaning a bacterium; Neo-Latin masculine gender noun ''Deinobacterium'', strange bacterium. Species The genus contains a single species, namely '' D. chartae'' ( Ekman ''et al''. 2011, (Type species of the genus).; Latin genitive case In grammar, the genitive case ( abbreviated ) is the grammatical case that marks a word, usually a noun, as modifying another word, also usually a noun—thus indicating an attributive relationship of one noun to the other noun. A genitive ca ... noun ''chartae'', of/from paper.) This bacterium is: * rod-shaped * non-spore-forming * non-motile, aerobic * oxidase and catalase-positive * radiation-r ...
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