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Colony Counter
In microbiology, a colony-forming unit (CFU, cfu or Cfu) is a unit which estimates the number of microbial cells (bacteria, fungi, viruses etc.) in a sample that are viable, able to multiply via binary fission under the controlled conditions. Counting with colony-forming units requires culturing the microbes and counts only viable cells, in contrast with microscopic examination which counts all cells, living or dead. The visual appearance of a colony in a cell culture requires significant growth, and when counting colonies, it is uncertain if the colony arose from a single cell or a group of cells. Expressing results as colony-forming units reflects this uncertainty. Theory The purpose of plate counting is to estimate the number of cells present based on their ability to give rise to colonies under specific conditions of temperature, time, and nutrient medium. Theoretically, one viable cell can give rise to a colony through replication. However, solitary cells are the exception i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microbiology
Microbiology () is the branches of science, scientific study of microorganisms, those being of unicellular organism, unicellular (single-celled), multicellular organism, multicellular (consisting of complex cells), or non-cellular life, acellular (lacking cells). Microbiology encompasses numerous sub-disciplines including virology, bacteriology, protistology, mycology, immunology, and parasitology. The organisms that constitute the microbial world are characterized as either prokaryotes or eukaryotes; eukaryote, Eukaryotic microorganisms possess membrane-bound organelles and include fungi and protists, whereas prokaryote, prokaryotic organisms are conventionally classified as lacking membrane-bound organelles and include Bacteria and Archaea. Microbiologists traditionally relied on culture, staining, and microscopy for the isolation and identification of microorganisms. However, less than 1% of the microorganisms present in common environments can be cultured in isolation using c ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Vortex Mixer
A vortex mixer, or vortexer, is a simple device used commonly in laboratories to mix small vials of liquid. It consists of an electric motor with the drive shaft oriented vertically and attached to a cupped rubber piece mounted slightly off-center. As the motor runs the rubber piece oscillates rapidly in a circular motion. When a test tube or other appropriate container is pressed into the rubber cup (or touched to its edge) the motion is transmitted to the liquid inside and a vortex is created. Most vortex mixers are designed with 2 or 4-plate formats, have variable speed settings ranging from 100 to 3,200 rpm, and can be set to run continuously, or to run only when downward pressure is applied to the rubber piece. Use Vortex mixers are quite commonplace in bioscience laboratories. In cell culture and microbiology laboratories they may be used to suspend cells. In a biochemical or analytical laboratory they may be used to mix the reagents of an assay or to mix an experime ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spiral Plater
A spiral plater is an instrument used to dispense a liquid sample onto a Petri dish in a spiral pattern. Commonly used as part of a Colony-forming unit, CFU count procedure for the purpose of determining the number of microbes in the sample.J. E. Gilchrist, J. E. Campbell, C. B. Donnelly, J. T. Peeler, and J. M. Delaney"Spiral Plate Method for Bacterial Determination" ''Appl Microbiol. '', 1973 February; 25(2): 244–252., In this setting, after spiral plating, the Petri dish is incubated for several hours after which the number of colony forming microbes (CFU) is determined. Spiral platers are also used for research, clinical diagnostics and as a method for covering a Petri dish with bacteria before placing antibiotic discs for Antibiotic sensitivity, AST. Mode of action The spiral plater rotates the dish while simultaneously dispensing the liquid and either linear motion, linearly moving the dish or the dispensing tip. This creates the common spiral pattern. If all movements ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Human Error
Human error is an action that has been done but that was "not intended by the actor; not desired by a set of rules or an external observer; or that led the task or system outside its acceptable limits".Senders, J.W. and Moray, N.P. (1991) Human Error: Cause, Prediction, and Reduction'. Lawrence Erlbaum Associates, p.25. . Human error has been cited as a primary cause and contributing factor in disasters and accidents in industries as diverse as Nuclear and radiation accidents and incidents, nuclear power (e.g., the Three Mile Island accident), Pilot error, aviation, List of spaceflight-related accidents and incidents, space exploration (e.g., the Space Shuttle Challenger disaster and Space Shuttle Columbia disaster), and Medical error, medicine. Prevention of human error is generally seen as a major contributor to Data integrity, reliability and safety of (complex) systems. Human error is one of the many contributing causes of risk events. Definition Human error refers to somethi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
CellProfiler
CellProfiler is free, open-source software designed to enable biologists without training in computer vision or programming to quantitatively measure phenotypes from thousands of images automatically. Advanced algorithms for image analysis are available as individual modules that can be placed in sequential order together to form a pipeline; the pipeline is then used to identify and measure biological objects and features in images, particularly those obtained through fluorescence microscopy. Distributions are available for Microsoft Windows, macOS, and Linux. The source code for CellProfiler is freely available. CellProfiler is developed by the Broad Institute's Imaging Platform. Features CellProfiler can read and analyze most common microscopy image formats. Biologists typically use CellProfiler to identify objects of interest (e.g. cells, colonies, ''C. elegans'' worms) and then measure their properties of interest. Specialized modules for illumination correction may be app ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
ImageJ
ImageJ is a Java (programming language), Java-based image processing program developed at the National Institutes of Health and the Laboratory for Optical and Computational Instrumentation (LOCI, University of Wisconsin). Its first version, ImageJ 1.x, is developed in the public domain, while ImageJ2 and the related projects SciJava, ImgLib2, and SCIFIO are licensed with a permissive BSD licenses, BSD-2 license. ImageJ was designed with an open architecture that provides extensibility via Java plug-in (computing), plugins and recordable macros. Custom acquisition, analysis and processing plugins can be developed using ImageJ's built-in editor and a Java compiler. User-written plugins make it possible to solve many image processing and analysis problems, from three-dimensional live-cell imaging to radiology, radiological image processing, multiple imaging system data comparisons to automated hematology systems. ImageJ's plugin architecture and built-in development environment has mad ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
MATLAB
MATLAB (an abbreviation of "MATrix LABoratory") is a proprietary multi-paradigm programming language and numeric computing environment developed by MathWorks. MATLAB allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces, and interfacing with programs written in other languages. Although MATLAB is intended primarily for numeric computing, an optional toolbox uses the MuPAD symbolic engine allowing access to symbolic computing abilities. An additional package, Simulink, adds graphical multi-domain simulation and model-based design for dynamic and embedded systems. , MATLAB has more than four million users worldwide. They come from various backgrounds of engineering, science, and economics. , more than 5000 global colleges and universities use MATLAB to support instruction and research. History Origins MATLAB was invented by mathematician and computer programmer Cleve Moler. The idea for MATLAB was base ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
OpenCV
OpenCV (Open Source Computer Vision Library) is a Library (computing), library of programming functions mainly for Real-time computing, real-time computer vision. Originally developed by Intel, it was later supported by Willow Garage, then Itseez (which was later acquired by Intel). The library is cross-platform and licensed as free and open-source software under Apache License 2. Starting in 2011, OpenCV features GPU acceleration for real-time operations. History Officially launched in 1999, the OpenCV project was initially an Intel Research Lablets, Intel Research initiative to advance central processing unit, CPU-intensive applications, part of a series of projects including Real-time computing, real-time ray tracing (graphics), ray tracing and 3D Display, 3D display walls. The main contributors to the project included a number of optimization experts in Intel Russia, as well as Intel's Performance Library Team. In the early days of OpenCV, the goals of the project were describ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Free And Open-source Software
Free and open-source software (FOSS) is software available under a license that grants users the right to use, modify, and distribute the software modified or not to everyone free of charge. FOSS is an inclusive umbrella term encompassing free software and open-source software. The rights guaranteed by FOSS originate from the "Four Essential Freedoms" of '' The Free Software Definition'' and the criteria of '' The Open Source Definition''. All FOSS can have publicly available source code, but not all source-available software is FOSS. FOSS is the opposite of proprietary software, which is licensed restrictively or has undisclosed source code. The historical precursor to FOSS was the hobbyist and academic public domain software ecosystem of the 1960s to 1980s. Free and open-source operating systems such as Linux distributions and descendants of BSD are widely used, powering millions of servers, desktops, smartphones, and other devices. Free-software licenses and open-so ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Manual CFU Counting
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Manual may refer to: Instructions * User guide * Owner's manual * Instruction manual (gaming) * Online help *Procedures manual *Handbook Other uses * Manual (music), a keyboard, as for an organ * Manual (band) * Manual transmission * Manual, a bicycle technique similar to a wheelie, but without the use of pedal torque * Manual, balancing on two wheels in freestyle skateboarding tricks * '' The Manual (How to Have a Number One the Easy Way)'' is a 1988 book by Bill Drummond and Jimmy Cauty See also * Instruction (other) * Tutorial In education, a tutorial is a method of transferring knowledge and may be used as a part of a learning process. More interactive and specific than a book or a lecture, a tutorial seeks to teach by example and supply the information to complete ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Miles And Misra Method
The Miles and Misra Method (or surface viable count) is a technique used in Microbiology to determine the number of colony forming units in a bacterial suspension or homogenate. The technique was first described in 1938 by Miles, Misra and Irwin who at the time were working at the LSHTM. The Miles and Misra method has been shown to be precise. Materials * A calibrated dropping pipette, or automatic pipette, delivering drops of 20μl. * Petri dishes containing nutrient agar or other appropriate medium. * Phosphate Buffered Saline (PBS) or other appropriate diluent. * Bacterial suspension or homogenate. Method *The inoculum / suspension is serially diluted by adding 1x of suspension to 9x of diluent. When the quantity of bacteria is unknown, dilutions should be made to at least 10−8. *Three plates are needed for each dilution series, for statistical reasons an average of at least 3 counts are needed. *The surface of the plates need to be sufficiently dry to allow a 20μl drop to ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
