Bead Beating
Cell disruption, sometimes referred to as ''digestion'', is a method or process for releasing biological molecules from inside a cell. Methods The production of biologically interesting molecules using cloning and culturing methods allows the study and manufacture of relevant molecules. Except for excreted molecules, cells producing molecules of interest must be disrupted. This page discusses various methods. Another method of disruption is called cell unroofing. Bead method A common laboratory-scale mechanical method for cell disruption uses glass, ceramic, or steel beads, in diameter, mixed with a sample suspended in an aqueous solution. First developed by Tim Hopkins in the late 1970s, the sample and bead mix is subjected to high level agitation by stirring or shaking. Beads collide with the cellular sample, cracking open the cell to release the intracellular components. Unlike some other methods, mechanical shear is moderate during homogenization resulting in excellen ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Cell Disruptor-Genie
Cell most often refers to: * Cell (biology), the functional basic unit of life * Cellphone, a phone connected to a cellular network * Clandestine cell, a penetration-resistant form of a secret or outlawed organization * Electrochemical cell, a device used to convert chemical energy to electrical energy * Prison cell, a room used to hold people in prisons Cell may also refer to: Arts, entertainment, and media Fictional entities * Cell (comics), a Marvel comic book character * Cell (''Dragon Ball''), a character in the manga series ''Dragon Ball'' Literature * ''Cell'' (novel), a 2006 horror novel by Stephen King * "Cells", poem, about a hungover soldier in gaol, by Rudyard Kipling * ''The Cell'' (play), an Australian play by Robert Wales Music * Cell (music), a small rhythmic and melodic design that can be isolated, or can make up one part of a thematic context * Cell (American band) * Cell (Japanese band) * ''Cell'' (album), a 2004 album by Plastic Tree * ''Cells'', a 199 ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Plant Tissue
In biology, tissue is an assembly of similar cells and their extracellular matrix from the same embryonic origin that together carry out a specific function. Tissues occupy a biological organizational level between cells and a complete organ. Accordingly, organs are formed by the functional grouping together of multiple tissues. The English word "tissue" derives from the French word "", the past participle of the verb tisser, "to weave". The study of tissues is known as histology or, in connection with disease, as histopathology. Xavier Bichat is considered as the "Father of Histology". Plant histology is studied in both plant anatomy and physiology. The classical tools for studying tissues are the paraffin block in which tissue is embedded and then sectioned, the histological stain, and the optical microscope. Developments in electron microscopy, immunofluorescence, and the use of frozen tissue-sections have enhanced the detail that can be observed in tissues. With th ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Fluorocarbon
Fluorocarbons are chemical compounds with carbon-fluorine bonds. Compounds that contain many C-F bonds often have distinctive properties, e.g., enhanced stability, volatility, and hydrophobicity. Several fluorocarbons and their derivatives are commercial polymers, refrigerants, drugs, and anesthetics. Nomenclature Perfluorocarbons or PFCs, are organofluorine compounds with the formula CxFy, meaning they contain only carbon and fluorine. The terminology is not strictly followed and many fluorine-containing organic compounds are also called fluorocarbons. Compounds with the prefix perfluoro- are hydrocarbons, including those with heteroatoms, wherein all C-H bonds have been replaced by C-F bonds. Fluorocarbons includes perfluoroalkanes, fluoroalkenes, fluoroalkynes, and perfluoroaromatic compounds. Perfluoroalkanes Chemical properties Perfluoroalkanes are very stable because of the strength of the carbon–fluorine bond, one of the strongest in organic chemistry. Its st ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Gaseous Coolant
A coolant is a substance, typically liquid, that is used to reduce or regulate the temperature of a system. An ideal coolant has high thermal capacity, low viscosity, is low-cost, non-toxic, chemically inert and neither causes nor promotes corrosion of the cooling system. Some applications also require the coolant to be an electrical insulator. While the term "coolant" is commonly used in automotive and HVAC applications, in industrial processing heat-transfer fluid is one technical term more often used in high temperature as well as low-temperature manufacturing applications. The term also covers cutting fluids. Industrial cutting fluid has broadly been classified as water-soluble coolant and neat cutting fluid. Water-soluble coolant is oil in water emulsion. It has varying oil content from nil oil (synthetic coolant). This coolant can either keep its phase and stay liquid or gaseous, or can undergo a phase transition, with the latent heat adding to the cooling efficiency. The la ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Vial Rack
A vial (also known as a phial or flacon) is a small glass or plastic vessel or bottle, often used to store medication in the form of liquids, powders, or capsules. They can also be used as scientific sample vessels; for instance, in autosampler devices in analytical chromatography. Vial-like glass containers date back to classical antiquity; modern vials are often made of plastics such as polypropylene. There are different types of vials such as a single dose vial and multi-dose vials often used for medications. The single dose vial is only used once whereas a multi-dose vial can be used more than once. The CDC sets specific guidelines on multi-dose vials. History and etymology A vial can be tubular, or have a bottle-like shape with a neck. The volume defined by the neck is known as the headspace. The English word "vial" is derived from the Greek ''phiale'', meaning "a broad flat container". Comparable terms include the Latin ''phiala'', Late Latin ''fiola'' and Middle Engl ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Aluminum
Aluminium (or aluminum in North American English) is a chemical element; it has chemical symbol, symbol Al and atomic number 13. It has a density lower than that of other common metals, about one-third that of steel. Aluminium has a great affinity towards oxygen, passivation (chemistry), forming a protective layer of aluminium oxide, oxide on the surface when exposed to air. It visually resembles silver, both in its color and in its great ability to reflect light. It is soft, magnetism, nonmagnetic, and ductility, ductile. It has one stable isotope, 27Al, which is highly abundant, making aluminium the abundance of the chemical elements, 12th-most abundant element in the universe. The radioactive decay, radioactivity of aluminium-26, 26Al leads to it being used in radiometric dating. Chemically, aluminium is a post-transition metal in the boron group; as is common for the group, aluminium forms compounds primarily in the +3 oxidation state. The aluminium cation Al3+ ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Microtiter Plate
A microplate, also known as a microtiter plate, microwell plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 12, 24, 48, 96, 384 or 1536 sample wells arranged in a 2:3 rectangular matrix. Some microplates have been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape. Each well of a microplate typically holds somewhere between tens of nanolitres to several millilitres of liquid. They can also be used to store dry powder or as racks to support glass tube inserts. Wells can be either circular or square. For compound storage applications, square we ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Electric Motor
An electric motor is a machine that converts electrical energy into mechanical energy. Most electric motors operate through the interaction between the motor's magnetic field and electric current in a electromagnetic coil, wire winding to generate Laplace force in the form of torque applied on the motor's shaft. An electric generator is mechanically identical to an electric motor, but operates in reverse, converting mechanical energy into electrical energy. Electric motors can be powered by direct current (DC) sources, such as from batteries or rectifiers, or by alternating current (AC) sources, such as a power grid, Inverter (electrical), inverters or electrical generators. Electric motors may also be classified by considerations such as power source type, construction, application and type of motion output. They can be brushed motor, brushed or brushless motor, brushless, single-phase electric power, single-phase, two-phase electric power, two-phase, or three-phase electric p ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Centrifuge Tube
A laboratory centrifuge is a piece of laboratory equipment, driven by a motor, which spins liquid samples at high speed. There are various types of centrifuges, depending on the size and the sample capacity.Susan R. Mikkelsen & Eduardo Cortón. Bioanalytical Chemistry, Ch. 13. Centrifugation Methods. John Wiley & Sons, Mar 4, 2004, pp. 247-267. Like all other centrifuges, laboratory centrifuges work by the sedimentation principle, where the centripetal acceleration is used to separate substances of greater and lesser density. Types There are various types of centrifugation: * Differential centrifugation, often used to separate certain organelles from whole cells for further analysis of specific parts of cells * Isopycnic centrifugation, often used to isolate nucleic acids such as DNA * Sucrose gradient centrifugation, often used to purify enveloped viruses and ribosomes, and also to separate cell organelles from crude cellular extracts There are different types of laboratory ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Vial
A vial (also known as a phial or flacon) is a small glass or plastic vessel or bottle, often used to store medication in the form of liquids, powders, or capsules. They can also be used as scientific sample vessels; for instance, in autosampler devices in analytical chromatography. Vial-like glass containers date back to classical antiquity; modern vials are often made of plastics such as polypropylene. There are different types of vials such as a single dose vial and multi-dose vials often used for medications. The single dose vial is only used once whereas a multi-dose vial can be used more than once. The CDC sets specific guidelines on multi-dose vials. History and etymology A vial can be tubular, or have a bottle-like shape with a neck. The volume defined by the neck is known as the headspace. The English word "vial" is derived from the Greek ''phiale'', meaning "a broad flat container". Comparable terms include the Latin ''phiala'', Late Latin ''fiola'' and Middle Eng ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Shaker (laboratory)
A shaker is a piece of laboratory equipment used to mix, blend, or agitate substances in a tube or flask by shaking them. It is mainly used in the fields of chemistry and biology. A shaker contains an oscillating board that is used to place the flasks, beakers, or test tubes. Although the magnetic stirrer has lately come to replace the shaker, it is still the preferred choice of equipment when dealing with large volume substances, when simultaneous agitation is required or when stirring bar could destroy delicate content like living cells. Types Vortex shaker Invented by Jack A. Kraft and Harold D. Kraft in 1962, a vortex shaker is a usually small device used to shake or mix small vials of liquid substance. Its most standout characteristic is that it works by the user putting a vial on the shaking platform and turning it on; thus, the vial is shaken along with the platform. A vortex shaker is very variable in terms of speed adjustment, for the shaking speed can be continuou ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Vortex Mixer
A vortex mixer, or vortexer, is a simple device used commonly in laboratories to mix small vials of liquid. It consists of an electric motor with the drive shaft oriented vertically and attached to a cupped rubber piece mounted slightly off-center. As the motor runs the rubber piece oscillates rapidly in a circular motion. When a test tube or other appropriate container is pressed into the rubber cup (or touched to its edge) the motion is transmitted to the liquid inside and a vortex is created. Most vortex mixers are designed with 2 or 4-plate formats, have variable speed settings ranging from 100 to 3,200 rpm, and can be set to run continuously, or to run only when downward pressure is applied to the rubber piece. Use Vortex mixers are quite commonplace in bioscience laboratories. In cell culture and microbiology laboratories they may be used to suspend cells. In a biochemical or analytical laboratory they may be used to mix the reagents of an assay or to mix an experime ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |